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The map label for this gene is purD

Identifier: 218930734

GI number: 218930734

Start: 4182767

End: 4184053

Strand: Direct

Name: purD

Synonym: YPO3729

Alternate gene names: 218930734

Gene position: 4182767-4184053 (Clockwise)

Preceding gene: 218930733

Following gene: 218930742

Centisome position: 89.88

GC content: 51.67

Gene sequence:

>1287_bases
ATGAATATTTTGATAATTGGTAACGGCGGTCGTGAACACGCTCTGGGCTGGAAAGCCGCCCAATCTCCTTTAGCGGACAA
AATTTATGTTGCACCAGGTAATGCGGGTACAGCACTGGAACCGACCTTAGAAAATGTTGATATCGCCGCCACTGATATTG
CCGGTTTACTGGCCTTTGCTCAAAGTCATGATATCGGCCTGACGATTGTTGGCCCAGAAGCCCCTTTGGTGATCGGCGTG
GTTGATGCGTTCCGCGCTGCTGGTTTAGCTATTTTTGGCCCGACTCAGGCTGCGGCTCAATTAGAGGGTTCTAAAGCCTT
CACCAAAGATTTCCTGGCCCGTCACAACATTCCCTCTGCGGAATACCAAAACTTTACAGATGTCGAGGCCGCATTGGCCT
ATGTGCGTCAAAAAGGTGCGCCAATCGTTATCAAAGCCGATGGTCTGGCCGCCGGTAAAGGCGTGATTGTTGCGATGACG
CAGGAAGAAGCCGAAACCGCCGTGAATGATATGTTGGCCGGTAACGCTTTTGGTGATGCAGGGCACCGTATCGTGGTGGA
AGAGTTCCTTGATGGCGAAGAAGCCAGCTTTATCGTGATGGTTGATGGCGAAAATGTTTTGCCAATGGCGACCAGTCAGG
ATCATAAGCGAGTTGGCGATGGTGATACCGGGCCAAATACCGGCGGAATGGGTGCTTATTCCCCAGCCCCCGTGGTAACA
GATGATGTTCACCAACGGGTCATGGATCAGGTTATTTGGCCGACCGTGCGTGGTATGGCGGCGGAAGGTAATATTTACAC
CGGTTTCCTCTATGCTGGCCTGATGATTTCAGCCGATGGGCAACCCAAAGTCATTGAGTTCAACTGCCGCTTTGGCGATC
CAGAAACGCAGCCAATCATGTTGCGTATGCGCTCCGATTTGGTCGAACTGTGTTTAGCCGGTACACAAGGCAAACTAAAT
GAAAAAACCTCAGACTGGGATGAGCGCCCATCACTGGGGGTCGTTTTAGCCGCTGGCGGTTATCCAGCAGATTACCGCCA
GGGTGATGTTATTCATGGCTTACCACAGCAAGAAGTCAAGGATGGAAAAGTCTTCCACGCGGGGACCAAGCTGAATGGGA
ATCATGAAGTTGTCACCAATGGTGGCCGCGTCTTGTGTGTCACTGCACTCGGTGAAACCGTCGCGCAGGCGCAACAATAT
GCCTATCAGTTAGCTGAGGGGATCCAGTGGGAAGGGGTTTTCTGCCGTAAAGATATTGGTTATCGAGCGATTGCTCGCGG
TAAGTAA

Upstream 100 bases:

>100_bases
TTGCCATGATCTTCACCGACATGCGCCATTTCCGTCATTAATTAACCCTCTTATATAGGAAGGGGCGCTTGATGATGTGC
CCCTTCACGGAGTCATCCAG

Downstream 100 bases:

>100_bases
AGGCTCACTGTCTTTAAAAGGGTACAGTCTAGTGCGCTGTGCCCTACTTATTGCGGATCATAATCGATTACTGGCTATCT
TATGACGCTGCACCGCTGAT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MNILIIGNGGREHALGWKAAQSPLADKIYVAPGNAGTALEPTLENVDIAATDIAGLLAFAQSHDIGLTIVGPEAPLVIGV
VDAFRAAGLAIFGPTQAAAQLEGSKAFTKDFLARHNIPSAEYQNFTDVEAALAYVRQKGAPIVIKADGLAAGKGVIVAMT
QEEAETAVNDMLAGNAFGDAGHRIVVEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
DDVHQRVMDQVIWPTVRGMAAEGNIYTGFLYAGLMISADGQPKVIEFNCRFGDPETQPIMLRMRSDLVELCLAGTQGKLN
EKTSDWDERPSLGVVLAAGGYPADYRQGDVIHGLPQQEVKDGKVFHAGTKLNGNHEVVTNGGRVLCVTALGETVAQAQQY
AYQLAEGIQWEGVFCRKDIGYRAIARGK

Sequences:

>Translated_428_residues
MNILIIGNGGREHALGWKAAQSPLADKIYVAPGNAGTALEPTLENVDIAATDIAGLLAFAQSHDIGLTIVGPEAPLVIGV
VDAFRAAGLAIFGPTQAAAQLEGSKAFTKDFLARHNIPSAEYQNFTDVEAALAYVRQKGAPIVIKADGLAAGKGVIVAMT
QEEAETAVNDMLAGNAFGDAGHRIVVEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
DDVHQRVMDQVIWPTVRGMAAEGNIYTGFLYAGLMISADGQPKVIEFNCRFGDPETQPIMLRMRSDLVELCLAGTQGKLN
EKTSDWDERPSLGVVLAAGGYPADYRQGDVIHGLPQQEVKDGKVFHAGTKLNGNHEVVTNGGRVLCVTALGETVAQAQQY
AYQLAEGIQWEGVFCRKDIGYRAIARGK
>Mature_428_residues
MNILIIGNGGREHALGWKAAQSPLADKIYVAPGNAGTALEPTLENVDIAATDIAGLLAFAQSHDIGLTIVGPEAPLVIGV
VDAFRAAGLAIFGPTQAAAQLEGSKAFTKDFLARHNIPSAEYQNFTDVEAALAYVRQKGAPIVIKADGLAAGKGVIVAMT
QEEAETAVNDMLAGNAFGDAGHRIVVEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
DDVHQRVMDQVIWPTVRGMAAEGNIYTGFLYAGLMISADGQPKVIEFNCRFGDPETQPIMLRMRSDLVELCLAGTQGKLN
EKTSDWDERPSLGVVLAAGGYPADYRQGDVIHGLPQQEVKDGKVFHAGTKLNGNHEVVTNGGRVLCVTALGETVAQAQQY
AYQLAEGIQWEGVFCRKDIGYRAIARGK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI28416899, Length=426, Percent_Identity=50, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI4503915, Length=426, Percent_Identity=50, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI209869995, Length=426, Percent_Identity=50, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI209869993, Length=426, Percent_Identity=50, Blast_Score=406, Evalue=1e-113,
Organism=Escherichia coli, GI1790438, Length=426, Percent_Identity=80.7511737089202, Blast_Score=712, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17567511, Length=427, Percent_Identity=40.9836065573771, Blast_Score=315, Evalue=4e-86,
Organism=Saccharomyces cerevisiae, GI6321203, Length=439, Percent_Identity=42.369020501139, Blast_Score=347, Evalue=3e-96,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=44.1860465116279, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=44.1860465116279, Blast_Score=336, Evalue=1e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR2_YERPE (Q8ZAR2)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AI0453
- RefSeq:   NP_667839.1
- RefSeq:   NP_994385.1
- RefSeq:   YP_002348609.1
- PDB:   3MJF
- PDBsum:   3MJF
- ProteinModelPortal:   Q8ZAR2
- SMR:   Q8ZAR2
- IntAct:   Q8ZAR2
- GeneID:   1145448
- GeneID:   1176555
- GeneID:   2765674
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO3729
- KEGG:   ypk:y0501
- KEGG:   ypm:YP_3092
- HOGENOM:   HBG404060
- OMA:   GVPIVIK
- ProtClustDB:   PRK00885
- BioCyc:   YPES187410:Y0501-MONOMER
- BioCyc:   YPES214092:YPO3729-MONOMER
- BRENDA:   6.3.4.13
- HAMAP:   MF_00138
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.90.600.10
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR00877

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.13

Molecular weight: Translated: 45318; Mature: 45318

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS50975 ATP_GRASP; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILIIGNGGREHALGWKAAQSPLADKIYVAPGNAGTALEPTLENVDIAATDIAGLLAFA
CEEEEEECCCCCCCCCCHHHCCCCCCEEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHH
QSHDIGLTIVGPEAPLVIGVVDAFRAAGLAIFGPTQAAAQLEGSKAFTKDFLARHNIPSA
CCCCCEEEEECCCCCEEEEEHHHHHHCCEEEECCCHHHHHCCCCHHHHHHHHHHCCCCCH
EYQNFTDVEAALAYVRQKGAPIVIKADGLAAGKGVIVAMTQEEAETAVNDMLAGNAFGDA
HCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
GHRIVVEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
CCEEEEHHHCCCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DDVHQRVMDQVIWPTVRGMAAEGNIYTGFLYAGLMISADGQPKVIEFNCRFGDPETQPIM
HHHHHHHHHHHHHHHHCCEEECCCEEEEEEEECEEEECCCCCCEEEEEECCCCCCCCHHH
LRMRSDLVELCLAGTQGKLNEKTSDWDERPSLGVVLAAGGYPADYRQGDVIHGLPQQEVK
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEECCCCHHHCC
DGKVFHAGTKLNGNHEVVTNGGRVLCVTALGETVAQAQQYAYQLAEGIQWEGVFCRKDIG
CCEEEEECCEECCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHCCCEECEEEEECCCC
YRAIARGK
CEEECCCC
>Mature Secondary Structure
MNILIIGNGGREHALGWKAAQSPLADKIYVAPGNAGTALEPTLENVDIAATDIAGLLAFA
CEEEEEECCCCCCCCCCHHHCCCCCCEEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHH
QSHDIGLTIVGPEAPLVIGVVDAFRAAGLAIFGPTQAAAQLEGSKAFTKDFLARHNIPSA
CCCCCEEEEECCCCCEEEEEHHHHHHCCEEEECCCHHHHHCCCCHHHHHHHHHHCCCCCH
EYQNFTDVEAALAYVRQKGAPIVIKADGLAAGKGVIVAMTQEEAETAVNDMLAGNAFGDA
HCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCC
GHRIVVEEFLDGEEASFIVMVDGENVLPMATSQDHKRVGDGDTGPNTGGMGAYSPAPVVT
CCEEEEHHHCCCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
DDVHQRVMDQVIWPTVRGMAAEGNIYTGFLYAGLMISADGQPKVIEFNCRFGDPETQPIM
HHHHHHHHHHHHHHHHCCEEECCCEEEEEEEECEEEECCCCCCEEEEEECCCCCCCCHHH
LRMRSDLVELCLAGTQGKLNEKTSDWDERPSLGVVLAAGGYPADYRQGDVIHGLPQQEVK
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEECCCCHHHCC
DGKVFHAGTKLNGNHEVVTNGGRVLCVTALGETVAQAQQYAYQLAEGIQWEGVFCRKDIG
CCEEEEECCEECCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHCCCEECEEEEECCCC
YRAIARGK
CEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430