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The map label for this gene is dusB [H]

Identifier: 218930665

GI number: 218930665

Start: 4073470

End: 4074435

Strand: Reverse

Name: dusB [H]

Synonym: YPO3655

Alternate gene names: 218930665

Gene position: 4074435-4073470 (Counterclockwise)

Preceding gene: 218930666

Following gene: 218930664

Centisome position: 87.55

GC content: 51.86

Gene sequence:

>966_bases
ATGCGTATTGGACACTTCCAGCTTACAAATTGCCTGATTGCCGCCCCGATGGCGGGTATCACAGATCGCCCCTTCAGAGC
GCTATGTCATGGTATGGGGGCTGGGATGGCTGTATCTGAAATGCTCTCCTCTAATCCAGAGGTTTGGCGAACGGATAAGT
CGCGTTTACGCATGGTCCATGTTGATGAACCCGGGATTCGTAACGTGCAAATTGCCGGTAACGATCCAGATGAGATGGCA
GCAGCCGCCAGAATCAATGTGGCGAGTGGTGCCCAAATCATCGATATCAATATGGGATGTCCAGCCAAGAAAGTGAACCG
CAAACTGGCGGGTTCTGCATTATTGCAGCATCCTGATCTGGTCAAACAGATCCTCTCCGCAGTGGTTAATGCGGTAGATG
TGCCGGTGACGTTGAAGATCCGGACGGGTTGGTCACCAGAACACCGTAACTGTATAGAAATTGCCCAACTGGCCGAAAAT
TGTGGTATACAAGCCCTGACGATTCATGGCCGAACCCGTTCTTGCCTGTTTAATGGTGAGGCGGAATACGACAGTATTCG
AGCAGTTAAGCAGACTGTTTCCATTCCCGTTATCGCGAATGGTGACATCACTGACCCGCATAAAGCCAGAGCGGTACTCG
ACTACACTGGAGCTGATGCTCTGATGATAGGCCGTGCCGCTCAGGGAAGACCGTGGATCTTCCGGGAAATCCAGCATTAT
CTGGACACTGGGGAACTGCTGCCACCGATGCCACTTGGCGAAGTGCAGCGTTTGTTAGACGGGCATATACGGGAATTGCA
CGACTTTTATGGTCCAGGCAAGGGATTTCGTATTGCACGTAAGCACGTCTCTTGGTATCTCCAAGAGCACGCCCCTAACG
ACCAGTTTCGGCGCACATTCAACGCCATTGAGGATGCCAGCGAACAGCTGGAGGCGTTGGAGGCATATTTCGAAAATCTT
GCGTAA

Upstream 100 bases:

>100_bases
GCTCTTTTTTCATCAATTGGTCAAAGTTTGGCCTTTCATCTGGGCGCAAAAATGCGTAATATACGCGCCCTTACGGACAC
AGTGTGGTCATTCTTTGTCT

Downstream 100 bases:

>100_bases
ACAGAAAAAAGAGCTGACAGAACTATGTTCGAACAACGCGTAAATTCTGACGTACTGACCGTTGCAACCGTAAACTCACA
AGATCAGGTGACTCAAAAGC

Product: tRNA-dihydrouridine synthase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MRIGHFQLTNCLIAAPMAGITDRPFRALCHGMGAGMAVSEMLSSNPEVWRTDKSRLRMVHVDEPGIRNVQIAGNDPDEMA
AAARINVASGAQIIDINMGCPAKKVNRKLAGSALLQHPDLVKQILSAVVNAVDVPVTLKIRTGWSPEHRNCIEIAQLAEN
CGIQALTIHGRTRSCLFNGEAEYDSIRAVKQTVSIPVIANGDITDPHKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
LDTGELLPPMPLGEVQRLLDGHIRELHDFYGPGKGFRIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENL
A

Sequences:

>Translated_321_residues
MRIGHFQLTNCLIAAPMAGITDRPFRALCHGMGAGMAVSEMLSSNPEVWRTDKSRLRMVHVDEPGIRNVQIAGNDPDEMA
AAARINVASGAQIIDINMGCPAKKVNRKLAGSALLQHPDLVKQILSAVVNAVDVPVTLKIRTGWSPEHRNCIEIAQLAEN
CGIQALTIHGRTRSCLFNGEAEYDSIRAVKQTVSIPVIANGDITDPHKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
LDTGELLPPMPLGEVQRLLDGHIRELHDFYGPGKGFRIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENL
A
>Mature_321_residues
MRIGHFQLTNCLIAAPMAGITDRPFRALCHGMGAGMAVSEMLSSNPEVWRTDKSRLRMVHVDEPGIRNVQIAGNDPDEMA
AAARINVASGAQIIDINMGCPAKKVNRKLAGSALLQHPDLVKQILSAVVNAVDVPVTLKIRTGWSPEHRNCIEIAQLAEN
CGIQALTIHGRTRSCLFNGEAEYDSIRAVKQTVSIPVIANGDITDPHKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
LDTGELLPPMPLGEVQRLLDGHIRELHDFYGPGKGFRIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENL
A

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=310, Percent_Identity=30.3225806451613, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI239788483, Length=231, Percent_Identity=31.1688311688312, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI239788462, Length=235, Percent_Identity=31.9148936170213, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI8923374, Length=251, Percent_Identity=31.8725099601594, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI40807366, Length=228, Percent_Identity=31.140350877193, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1789660, Length=321, Percent_Identity=87.8504672897196, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI1788462, Length=199, Percent_Identity=35.678391959799, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI145693211, Length=246, Percent_Identity=26.0162601626016, Blast_Score=64, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17507177, Length=244, Percent_Identity=30.327868852459, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17543114, Length=234, Percent_Identity=32.0512820512821, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17510279, Length=244, Percent_Identity=31.5573770491803, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI25144369, Length=231, Percent_Identity=31.6017316017316, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6323433, Length=262, Percent_Identity=30.5343511450382, Blast_Score=94, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323560, Length=231, Percent_Identity=29.4372294372294, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323437, Length=228, Percent_Identity=28.5087719298246, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24585320, Length=234, Percent_Identity=29.0598290598291, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24580595, Length=231, Percent_Identity=32.9004329004329, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI19920448, Length=231, Percent_Identity=32.9004329004329, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI45549423, Length=245, Percent_Identity=32.2448979591837, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19921524, Length=312, Percent_Identity=25.9615384615385, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 35467; Mature: 35467

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGHFQLTNCLIAAPMAGITDRPFRALCHGMGAGMAVSEMLSSNPEVWRTDKSRLRMVH
CCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEECCHHHEEEEE
VDEPGIRNVQIAGNDPDEMAAAARINVASGAQIIDINMGCPAKKVNRKLAGSALLQHPDL
CCCCCCCEEEECCCCHHHHHHHHEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHH
VKQILSAVVNAVDVPVTLKIRTGWSPEHRNCIEIAQLAENCGIQALTIHGRTRSCLFNGE
HHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCEEEECCC
AEYDSIRAVKQTVSIPVIANGDITDPHKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
CCHHHHHHHHHHHCCEEEECCCCCCCHHHCEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LDTGELLPPMPLGEVQRLLDGHIRELHDFYGPGKGFRIARKHVSWYLQEHAPNDQFRRTF
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
NAIEDASEQLEALEAYFENLA
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIGHFQLTNCLIAAPMAGITDRPFRALCHGMGAGMAVSEMLSSNPEVWRTDKSRLRMVH
CCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEECCHHHEEEEE
VDEPGIRNVQIAGNDPDEMAAAARINVASGAQIIDINMGCPAKKVNRKLAGSALLQHPDL
CCCCCCCEEEECCCCHHHHHHHHEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHCCHHH
VKQILSAVVNAVDVPVTLKIRTGWSPEHRNCIEIAQLAENCGIQALTIHGRTRSCLFNGE
HHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCEEEECCC
AEYDSIRAVKQTVSIPVIANGDITDPHKARAVLDYTGADALMIGRAAQGRPWIFREIQHY
CCHHHHHHHHHHHCCEEEECCCCCCCHHHCEEEEECCCCEEEECCCCCCCCHHHHHHHHH
LDTGELLPPMPLGEVQRLLDGHIRELHDFYGPGKGFRIARKHVSWYLQEHAPNDQFRRTF
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHH
NAIEDASEQLEALEAYFENLA
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9811652 [H]