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The map label for this gene is basS [H]

Identifier: 218930522

GI number: 218930522

Start: 3918214

End: 3919281

Strand: Direct

Name: basS [H]

Synonym: YPO3508

Alternate gene names: 218930522

Gene position: 3918214-3919281 (Clockwise)

Preceding gene: 218930521

Following gene: 218930527

Centisome position: 84.2

GC content: 46.82

Gene sequence:

>1068_bases
ATGATCAGCATGCGCCGTCGTTTGCTGTTAATGCTGGCGCTGATTTTACTGGTGACCCAGTTGATCAGCGCATTCTGGCT
ATGGCATGAAAGTCAGGAACAAATAAGTTTTCTGGTCGATGAAACTCTTAGCGCAAAAGCCCGTAATGAGCAGGTTGATA
AAGAAATAGCTGAGGCGATTGCCTCCCTGCTTGCACCGTCGCTAATCATGATGACAATCACTCTGCTGCTGTCTTTCTGG
GCAATCAGTTGGATTATTCGTCCACTTGATCAATTACAACAAAAGTTGGCAGAACGCTCCGCAGATAACCTTTCCCCCTT
GGTTGTTAACAGTGAAATGCAAGAAATTGTCTCTGTTACCTCAACACTCAATCAATTGTTATTGCGACTTTCTAATACCA
TTCAGCAAGAACGGTTGTTTACCGCCGATGCCGCCCATGAACTGCGTACACCACTCGCAGGAATACGCCTCCACCTAGAA
TTAATGGAAAAGCAGGGAATTGCCGAGAGTAAATCGCTGATTAATCGCATCGATCTGCTAATGCATACGATTGAACAGTT
ATTAATGCTATCGAGGGCGGGACAAAATTTTGCTAGTGGCCATTATCAAACCCTAGATTGGGTTAACGATGTCGTCCCCC
CCTTGCAGGAAGAGCTGGCTGAAATGTGTGAGTTGCGTCGGCAAACAATACAGTGGGAATTACCGCCTCATGCAAAAATG
GAAGGTGACGCCACGCTACTACGCTTGATGCTGCGTAATTTGGTTGAAAATGCCCACCGCTATAGCCCGGTTGGTAGCCA
AATCATCGTCACTCTTTCCACTGAAAATCAGGGTGTATTACTGCAAGTCACCGATGAAGGCCCAGGGATAAAACAAAATC
AGGCTGGTGAACTAACCCAAGCATTTAGGCGCATGGACCAACGCTATGGTGGCAGTGGCTTAGGATTGAATATCGTGATC
CGCATTGCCCAGTTACATCAAGGTAAACTTACGTTGGAAAACCGCCTTGATCGTACTGGTCTGAAGGCACAATGCTGGCT
ACCCGCGACCACTTACAGCCAGAAATAG

Upstream 100 bases:

>100_bases
CTAATACGCTGGAAGTTCATATTCATAATTTGCGACGCAAACTGGGTAAAGATCGTATCCGAACCGTTCGAGGTATTGGT
TATCGATTGGAAGCCTTATC

Downstream 100 bases:

>100_bases
ATATGCTCACCGCCCCCAATAAGGGCCACCCTGCCAAAAAATAAGGGCCACCACGAAAAAATGGCAGCCCTTATTCAAAG
AATAACTGGAGAATTTAACG

Product: sensor protein BasS/PmrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MISMRRRLLLMLALILLVTQLISAFWLWHESQEQISFLVDETLSAKARNEQVDKEIAEAIASLLAPSLIMMTITLLLSFW
AISWIIRPLDQLQQKLAERSADNLSPLVVNSEMQEIVSVTSTLNQLLLRLSNTIQQERLFTADAAHELRTPLAGIRLHLE
LMEKQGIAESKSLINRIDLLMHTIEQLLMLSRAGQNFASGHYQTLDWVNDVVPPLQEELAEMCELRRQTIQWELPPHAKM
EGDATLLRLMLRNLVENAHRYSPVGSQIIVTLSTENQGVLLQVTDEGPGIKQNQAGELTQAFRRMDQRYGGSGLGLNIVI
RIAQLHQGKLTLENRLDRTGLKAQCWLPATTYSQK

Sequences:

>Translated_355_residues
MISMRRRLLLMLALILLVTQLISAFWLWHESQEQISFLVDETLSAKARNEQVDKEIAEAIASLLAPSLIMMTITLLLSFW
AISWIIRPLDQLQQKLAERSADNLSPLVVNSEMQEIVSVTSTLNQLLLRLSNTIQQERLFTADAAHELRTPLAGIRLHLE
LMEKQGIAESKSLINRIDLLMHTIEQLLMLSRAGQNFASGHYQTLDWVNDVVPPLQEELAEMCELRRQTIQWELPPHAKM
EGDATLLRLMLRNLVENAHRYSPVGSQIIVTLSTENQGVLLQVTDEGPGIKQNQAGELTQAFRRMDQRYGGSGLGLNIVI
RIAQLHQGKLTLENRLDRTGLKAQCWLPATTYSQK
>Mature_355_residues
MISMRRRLLLMLALILLVTQLISAFWLWHESQEQISFLVDETLSAKARNEQVDKEIAEAIASLLAPSLIMMTITLLLSFW
AISWIIRPLDQLQQKLAERSADNLSPLVVNSEMQEIVSVTSTLNQLLLRLSNTIQQERLFTADAAHELRTPLAGIRLHLE
LMEKQGIAESKSLINRIDLLMHTIEQLLMLSRAGQNFASGHYQTLDWVNDVVPPLQEELAEMCELRRQTIQWELPPHAKM
EGDATLLRLMLRNLVENAHRYSPVGSQIIVTLSTENQGVLLQVTDEGPGIKQNQAGELTQAFRRMDQRYGGSGLGLNIVI
RIAQLHQGKLTLENRLDRTGLKAQCWLPATTYSQK

Specific function: Member of the two-component regulatory system pmrB/pmrA involved in regulation of virulence. Functions probably as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phosphate group to pmrA in response to external

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790551, Length=354, Percent_Identity=49.7175141242938, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI1789403, Length=302, Percent_Identity=32.4503311258278, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789808, Length=288, Percent_Identity=29.1666666666667, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1786600, Length=270, Percent_Identity=30.7407407407407, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788393, Length=258, Percent_Identity=27.5193798449612, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1786783, Length=302, Percent_Identity=29.4701986754967, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790346, Length=244, Percent_Identity=29.9180327868852, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788279, Length=238, Percent_Identity=31.0924369747899, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787894, Length=272, Percent_Identity=28.6764705882353, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI87082128, Length=321, Percent_Identity=27.7258566978193, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 40223; Mature: 40223

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISMRRRLLLMLALILLVTQLISAFWLWHESQEQISFLVDETLSAKARNEQVDKEIAEAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASLLAPSLIMMTITLLLSFWAISWIIRPLDQLQQKLAERSADNLSPLVVNSEMQEIVSVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHH
STLNQLLLRLSNTIQQERLFTADAAHELRTPLAGIRLHLELMEKQGIAESKSLINRIDLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
MHTIEQLLMLSRAGQNFASGHYQTLDWVNDVVPPLQEELAEMCELRRQTIQWELPPHAKM
HHHHHHHHHHHHCCCCHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
EGDATLLRLMLRNLVENAHRYSPVGSQIIVTLSTENQGVLLQVTDEGPGIKQNQAGELTQ
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCHHHHH
AFRRMDQRYGGSGLGLNIVIRIAQLHQGKLTLENRLDRTGLKAQCWLPATTYSQK
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCCCCCCC
>Mature Secondary Structure
MISMRRRLLLMLALILLVTQLISAFWLWHESQEQISFLVDETLSAKARNEQVDKEIAEAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASLLAPSLIMMTITLLLSFWAISWIIRPLDQLQQKLAERSADNLSPLVVNSEMQEIVSVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHH
STLNQLLLRLSNTIQQERLFTADAAHELRTPLAGIRLHLELMEKQGIAESKSLINRIDLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
MHTIEQLLMLSRAGQNFASGHYQTLDWVNDVVPPLQEELAEMCELRRQTIQWELPPHAKM
HHHHHHHHHHHHCCCCHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
EGDATLLRLMLRNLVENAHRYSPVGSQIIVTLSTENQGVLLQVTDEGPGIKQNQAGELTQ
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEECCCCCCCCCCCCHHHHH
AFRRMDQRYGGSGLGLNIVIRIAQLHQGKLTLENRLDRTGLKAQCWLPATTYSQK
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 14617142 [H]