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The map label for this gene is ispB [H]

Identifier: 218930527

GI number: 218930527

Start: 3922471

End: 3923442

Strand: Direct

Name: ispB [H]

Synonym: YPO3513

Alternate gene names: 218930527

Gene position: 3922471-3923442 (Clockwise)

Preceding gene: 218930522

Following gene: 218930531

Centisome position: 84.29

GC content: 48.97

Gene sequence:

>972_bases
ATGAACCTAGAAAAGATTATCGAGTTAACCGCGCCAGATATGGCGGCAGTAAATACAACAATTCTCGACCAATTGAACTC
TGATGTTGTTCTTATCAATCAATTGGGTCATTACATTATCAGCGGTGGTGGAAAACGTATTCGCCCAATGATCTCGGTCT
TGGTGGCCCGGGCCCTTAACTATCAGGGTAGCAAGCATATCACCGTCGCGGCACTCATCGAGTTTATCCATACGGCGACA
CTGCTACACGATGATGTTGTCGATGAATCCGATATGCGGCGTGGCAAAGCGACCGCCAATGCCGCATTTGGCAACGCTGC
CAGTGTATTGGTCGGCGATTACATTTATACGCGCTCATTCCAGATGATGACAGGGCTGCAATCAATGCGAGTGCTGACAT
TGATGTCTGAAGCGACAAACGTTATCGCCGAAGGTGAGGTATTACAGTTAATGAACTGTAATAACCCAGACATCACTGAA
GAAAGCTATATGCAGGTCATTTACAGCAAAACAGCACGCTTATTCGAGGCCGCTGCACAATCTGCCGCAATATTGTCAGA
TGCCAGTGAGGAGCAGGAACTGGCATTACAGAACTATGGTCGTTATCTGGGGACTGCCTTCCAGCTTATTGATGATCTAC
TGGATTACAGCGCAGAGGGTTCCACGTTAGGCAAAAATACGGGGGATGACCTGAATGAAGGTAAACCGACGTTGCCACTG
CTACATGCCATGCATAATGGCACCCCAGAACAAGCCGCAATGATCCGGCAAGCGATTGAACAGGGGAATGGCCGTCATTT
ACTCGAACCAGTGCTGGCTGCCATGGAGCATTGTGGCTCTCTGGTCTATACCCGTCAGCGTGCTGAAGAAGAAGCAGATA
AAGCGATTGCCGCATTGCACGCGTTACCTGAAAGTGATTATCGTCTGGCCCTTGAAGGCTTGGCACATATCTCAGTGCAC
CGCCGTTTTTGA

Upstream 100 bases:

>100_bases
GATGCATTTTTCAAGTACAATCATGGTCTCAGAGAACGTCATGTAATAATGTGAGCGGTAATCTCATCGTAGTTAAAGAG
AAGAACAGCTGAATAAAACA

Downstream 100 bases:

>100_bases
TTATAGTTTATGGAAATTATTTAGAAACCCGGATTCACCATCCGGGTTTATCCCATTAATGAGTAAGCGCGGTCATATCC
TATCTTTACCCACGGGTTAA

Product: octaprenyl diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MNLEKIIELTAPDMAAVNTTILDQLNSDVVLINQLGHYIISGGGKRIRPMISVLVARALNYQGSKHITVAALIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDYIYTRSFQMMTGLQSMRVLTLMSEATNVIAEGEVLQLMNCNNPDITE
ESYMQVIYSKTARLFEAAAQSAAILSDASEEQELALQNYGRYLGTAFQLIDDLLDYSAEGSTLGKNTGDDLNEGKPTLPL
LHAMHNGTPEQAAMIRQAIEQGNGRHLLEPVLAAMEHCGSLVYTRQRAEEEADKAIAALHALPESDYRLALEGLAHISVH
RRF

Sequences:

>Translated_323_residues
MNLEKIIELTAPDMAAVNTTILDQLNSDVVLINQLGHYIISGGGKRIRPMISVLVARALNYQGSKHITVAALIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDYIYTRSFQMMTGLQSMRVLTLMSEATNVIAEGEVLQLMNCNNPDITE
ESYMQVIYSKTARLFEAAAQSAAILSDASEEQELALQNYGRYLGTAFQLIDDLLDYSAEGSTLGKNTGDDLNEGKPTLPL
LHAMHNGTPEQAAMIRQAIEQGNGRHLLEPVLAAMEHCGSLVYTRQRAEEEADKAIAALHALPESDYRLALEGLAHISVH
RRF
>Mature_323_residues
MNLEKIIELTAPDMAAVNTTILDQLNSDVVLINQLGHYIISGGGKRIRPMISVLVARALNYQGSKHITVAALIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDYIYTRSFQMMTGLQSMRVLTLMSEATNVIAEGEVLQLMNCNNPDITE
ESYMQVIYSKTARLFEAAAQSAAILSDASEEQELALQNYGRYLGTAFQLIDDLLDYSAEGSTLGKNTGDDLNEGKPTLPL
LHAMHNGTPEQAAMIRQAIEQGNGRHLLEPVLAAMEHCGSLVYTRQRAEEEADKAIAALHALPESDYRLALEGLAHISVH
RRF

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=309, Percent_Identity=31.7152103559871, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI4758430, Length=268, Percent_Identity=26.4925373134328, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI83700220, Length=268, Percent_Identity=26.4925373134328, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1789578, Length=321, Percent_Identity=79.4392523364486, Blast_Score=527, Evalue=1e-151,
Organism=Escherichia coli, GI1786623, Length=252, Percent_Identity=28.1746031746032, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17505681, Length=304, Percent_Identity=31.9078947368421, Blast_Score=155, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6319475, Length=303, Percent_Identity=33.993399339934, Blast_Score=146, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6325188, Length=269, Percent_Identity=25.2788104089219, Blast_Score=90, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24651612, Length=300, Percent_Identity=32.6666666666667, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI281365769, Length=287, Percent_Identity=26.8292682926829, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24660002, Length=287, Percent_Identity=26.8292682926829, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35313; Mature: 35313

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLEKIIELTAPDMAAVNTTILDQLNSDVVLINQLGHYIISGGGKRIRPMISVLVARALN
CCHHHHHHHCCCCHHHHHHHHHHHCCCCEEEHHHHCHHEECCCCHHHHHHHHHHHHHHHC
YQGSKHITVAALIEFIHTATLLHDDVVDESDMRRGKATANAAFGNAASVLVGDYIYTRSF
CCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHH
QMMTGLQSMRVLTLMSEATNVIAEGEVLQLMNCNNPDITEESYMQVIYSKTARLFEAAAQ
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SAAILSDASEEQELALQNYGRYLGTAFQLIDDLLDYSAEGSTLGKNTGDDLNEGKPTLPL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
LHAMHNGTPEQAAMIRQAIEQGNGRHLLEPVLAAMEHCGSLVYTRQRAEEEADKAIAALH
HHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALPESDYRLALEGLAHISVHRRF
HCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLEKIIELTAPDMAAVNTTILDQLNSDVVLINQLGHYIISGGGKRIRPMISVLVARALN
CCHHHHHHHCCCCHHHHHHHHHHHCCCCEEEHHHHCHHEECCCCHHHHHHHHHHHHHHHC
YQGSKHITVAALIEFIHTATLLHDDVVDESDMRRGKATANAAFGNAASVLVGDYIYTRSF
CCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHH
QMMTGLQSMRVLTLMSEATNVIAEGEVLQLMNCNNPDITEESYMQVIYSKTARLFEAAAQ
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
SAAILSDASEEQELALQNYGRYLGTAFQLIDDLLDYSAEGSTLGKNTGDDLNEGKPTLPL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
LHAMHNGTPEQAAMIRQAIEQGNGRHLLEPVLAAMEHCGSLVYTRQRAEEEADKAIAALH
HHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALPESDYRLALEGLAHISVHRRF
HCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]