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The map label for this gene is nusA [H]

Identifier: 218930511

GI number: 218930511

Start: 3906825

End: 3908312

Strand: Reverse

Name: nusA [H]

Synonym: YPO3497

Alternate gene names: 218930511

Gene position: 3908312-3906825 (Counterclockwise)

Preceding gene: 218930512

Following gene: 218930510

Centisome position: 83.98

GC content: 49.13

Gene sequence:

>1488_bases
ATGAACAAAGAGATTCTGGCTGTTGTAGAAGCAGTTTCCAATGAGAAATCCCTTCCGCGCGAGAAGATTTTTGAGGCGTT
GGAAACCGCTCTAGCGACAGCAACCAAGAAAAAATACGAACAAGAGATTGAAGTCCGCGTCAGTATTGACCGTAAAACCG
GTGATTTTGATACTTTCCGCCGTTGGGTCGCTGTTGACGAAGTGACTATGCCAACCCGTGAAATCACGTTGGATGCGGCT
CAATTTGAAGATCCTTCTCTCCAATTGGGTGATTATGTCGAAGACCAGATTGAATCGGTGACTTTTGACCGCATTACCAC
CCAAACAGCCAAGCAAGTCATCGTACAAAAAGTACGTGAAGCTGAACGGGCGATGGTTGTTGAGCAGTTCCGTCAATATC
TGGGGCAAATTGTCACTGGTATTGTTAAGAAAGTTAGCCGTGACAGTATTGCACTGGATCTGGGCCACAATGCGGAAGCT
GTTATTGGTCGTGAAGATATGCTCCCGCGTGAAAATTTCCGCCCAGGTGACCGTATCCGTGGTGTTCTGTATGACGTGCG
TCCAGAAGCTCGTGGCGCACAGTTGTTTGTCAGCCGTTCACGTTCTGAAATGTTGGTCGAACTGTTCCGCATTGAAGTAC
CAGAAATTGGTGAAGAGCTGATCGAAATTAAAGCCGCTGCCCGTGATCCTGGCTCTCGTGCTAAAATTGCGGTCAAAACC
AATGACAAGCGTATCGATCCGGTTGGTGCTTGCGTTGGTATGCGTGGTGCCCGTGTTCAGGCTGTGTCCAGCGAGCTTGG
CGGCGAGCGCATTGATATTGTATTGTGGGATGATAATCCAGCCCAGTTTGTTATTAACGCTATGGCGCCAGCTGATGTTG
CGTCTATTGTGGTTGATGAAGACAAACACACGATGGATGTTGCCGTTGAAGCCAGTAATTTGGCCCAGGCAATTGGTCGT
AATGGCCAGAACGTACGTTTAGCCGCGCAGCTTAGTGGCTGGGAACTGAACGTAATGACGGCGGACGATCTTCAGGCGAA
GCATCAGGCCGAGGCTCATGCCGCTATTGATACCTTCACTAAATATCTTGATATCGATGAAGACTTTGCCACCGTATTGG
TAGAAGAAGGTTTCTCTTCTCTGGAAGAGTTGGCTTATGTGCCAATGAAAGAACTTCTGGAAATCGATGGTCTTGACGAA
GATACGGTTGAAGCGCTGCGTGATCGCGCCAAAGCTGCATTGACCACGCTGGCCCTGGCACAAGAAGAAAGTTTTGGCGA
CCAGAAACCCGCTGATGACCTGTTGAATTTAGCGGGTCTGGAACGTAGCATGGCATTCAAATTGGCTGCGCGCGGTGTAT
GTACGCTGGAAGATCTTGCCGAGCAGGGTATCGACGATTTGGCAGATATTGAAGGGCTTAGCGATGAGCAAGCCGGTGAG
CTGATTATGGCCGCACGTAATATCTGTTGGTTTGGCGATAATGCGTAA

Upstream 100 bases:

>100_bases
CACGGTTACTGTGGATGGAAAAGATGAAGTGTTCGCGCTGAGCAACATCCAGAAAGCGAACCTGGTACCCCACTTTTAAA
GTTTGGATGAGGCAACTAGG

Downstream 100 bases:

>100_bases
TAAACTGTAGCAGGAAGGAACAGCATGACAGATGTAACCGTAAAATCGCTGGCAGCAGAGATTCAGACCCCGGTTGATCG
CCTGGTACAGCAGTTTGCTG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: L factor; N utilization substance protein A [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MNKEILAVVEAVSNEKSLPREKIFEALETALATATKKKYEQEIEVRVSIDRKTGDFDTFRRWVAVDEVTMPTREITLDAA
QFEDPSLQLGDYVEDQIESVTFDRITTQTAKQVIVQKVREAERAMVVEQFRQYLGQIVTGIVKKVSRDSIALDLGHNAEA
VIGREDMLPRENFRPGDRIRGVLYDVRPEARGAQLFVSRSRSEMLVELFRIEVPEIGEELIEIKAAARDPGSRAKIAVKT
NDKRIDPVGACVGMRGARVQAVSSELGGERIDIVLWDDNPAQFVINAMAPADVASIVVDEDKHTMDVAVEASNLAQAIGR
NGQNVRLAAQLSGWELNVMTADDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSSLEELAYVPMKELLEIDGLDE
DTVEALRDRAKAALTTLALAQEESFGDQKPADDLLNLAGLERSMAFKLAARGVCTLEDLAEQGIDDLADIEGLSDEQAGE
LIMAARNICWFGDNA

Sequences:

>Translated_495_residues
MNKEILAVVEAVSNEKSLPREKIFEALETALATATKKKYEQEIEVRVSIDRKTGDFDTFRRWVAVDEVTMPTREITLDAA
QFEDPSLQLGDYVEDQIESVTFDRITTQTAKQVIVQKVREAERAMVVEQFRQYLGQIVTGIVKKVSRDSIALDLGHNAEA
VIGREDMLPRENFRPGDRIRGVLYDVRPEARGAQLFVSRSRSEMLVELFRIEVPEIGEELIEIKAAARDPGSRAKIAVKT
NDKRIDPVGACVGMRGARVQAVSSELGGERIDIVLWDDNPAQFVINAMAPADVASIVVDEDKHTMDVAVEASNLAQAIGR
NGQNVRLAAQLSGWELNVMTADDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSSLEELAYVPMKELLEIDGLDE
DTVEALRDRAKAALTTLALAQEESFGDQKPADDLLNLAGLERSMAFKLAARGVCTLEDLAEQGIDDLADIEGLSDEQAGE
LIMAARNICWFGDNA
>Mature_495_residues
MNKEILAVVEAVSNEKSLPREKIFEALETALATATKKKYEQEIEVRVSIDRKTGDFDTFRRWVAVDEVTMPTREITLDAA
QFEDPSLQLGDYVEDQIESVTFDRITTQTAKQVIVQKVREAERAMVVEQFRQYLGQIVTGIVKKVSRDSIALDLGHNAEA
VIGREDMLPRENFRPGDRIRGVLYDVRPEARGAQLFVSRSRSEMLVELFRIEVPEIGEELIEIKAAARDPGSRAKIAVKT
NDKRIDPVGACVGMRGARVQAVSSELGGERIDIVLWDDNPAQFVINAMAPADVASIVVDEDKHTMDVAVEASNLAQAIGR
NGQNVRLAAQLSGWELNVMTADDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSSLEELAYVPMKELLEIDGLDE
DTVEALRDRAKAALTTLALAQEESFGDQKPADDLLNLAGLERSMAFKLAARGVCTLEDLAEQGIDDLADIEGLSDEQAGE
LIMAARNICWFGDNA

Specific function: Participates in both the termination and antitermination of transcription. NusA binds directly to the core enzyme of the DNA-dependent RNA polymerase. NusA also interacts with lambda N protein, RNA, rho factor, and perhaps nusB. Binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=495, Percent_Identity=88.4848484848485, Blast_Score=899, Evalue=0.0,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 54709; Mature: 54709

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKEILAVVEAVSNEKSLPREKIFEALETALATATKKKYEQEIEVRVSIDRKTGDFDTFR
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCHHHHH
RWVAVDEVTMPTREITLDAAQFEDPSLQLGDYVEDQIESVTFDRITTQTAKQVIVQKVRE
HHHHHHCCCCCCHHEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AERAMVVEQFRQYLGQIVTGIVKKVSRDSIALDLGHNAEAVIGREDMLPRENFRPGDRIR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEECHHHCCCCCCCCCCHHHC
GVLYDVRPEARGAQLFVSRSRSEMLVELFRIEVPEIGEELIEIKAAARDPGSRAKIAVKT
EEEEECCCCCCCCHHEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEEC
NDKRIDPVGACVGMRGARVQAVSSELGGERIDIVLWDDNPAQFVINAMAPADVASIVVDE
CCCCCCHHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCCHHEEEECCCCCHHHHEEECC
DKHTMDVAVEASNLAQAIGRNGQNVRLAAQLSGWELNVMTADDLQAKHQAEAHAAIDTFT
CCCEEEEEEEHHHHHHHHCCCCCCEEEEEEECCEEEEEEEHHHHHHHHHHHHHHHHHHHH
KYLDIDEDFATVLVEEGFSSLEELAYVPMKELLEIDGLDEDTVEALRDRAKAALTTLALA
HHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
QEESFGDQKPADDLLNLAGLERSMAFKLAARGVCTLEDLAEQGIDDLADIEGLSDEQAGE
HHHCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHCCCCCHHHHH
LIMAARNICWFGDNA
HHHHHCCEEECCCCC
>Mature Secondary Structure
MNKEILAVVEAVSNEKSLPREKIFEALETALATATKKKYEQEIEVRVSIDRKTGDFDTFR
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCHHHHH
RWVAVDEVTMPTREITLDAAQFEDPSLQLGDYVEDQIESVTFDRITTQTAKQVIVQKVRE
HHHHHHCCCCCCHHEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AERAMVVEQFRQYLGQIVTGIVKKVSRDSIALDLGHNAEAVIGREDMLPRENFRPGDRIR
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEECHHHCCCCCCCCCCHHHC
GVLYDVRPEARGAQLFVSRSRSEMLVELFRIEVPEIGEELIEIKAAARDPGSRAKIAVKT
EEEEECCCCCCCCHHEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEEEEC
NDKRIDPVGACVGMRGARVQAVSSELGGERIDIVLWDDNPAQFVINAMAPADVASIVVDE
CCCCCCHHHHHHCCCCCHHHHHHHHCCCCEEEEEEECCCCHHEEEECCCCCHHHHEEECC
DKHTMDVAVEASNLAQAIGRNGQNVRLAAQLSGWELNVMTADDLQAKHQAEAHAAIDTFT
CCCEEEEEEEHHHHHHHHCCCCCCEEEEEEECCEEEEEEEHHHHHHHHHHHHHHHHHHHH
KYLDIDEDFATVLVEEGFSSLEELAYVPMKELLEIDGLDEDTVEALRDRAKAALTTLALA
HHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
QEESFGDQKPADDLLNLAGLERSMAFKLAARGVCTLEDLAEQGIDDLADIEGLSDEQAGE
HHHCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHCCCCCHHHHH
LIMAARNICWFGDNA
HHHHHCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113180; 11677609 [H]