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The map label for this gene is folD [H]

Identifier: 218929889

GI number: 218929889

Start: 3151967

End: 3152833

Strand: Direct

Name: folD [H]

Synonym: YPO2824

Alternate gene names: 218929889

Gene position: 3151967-3152833 (Clockwise)

Preceding gene: 218929888

Following gene: 218929890

Centisome position: 67.73

GC content: 51.44

Gene sequence:

>867_bases
ATGTCAGCAAAAATTATTGATGGTAAAACGATTGCGCAGCAGGTAAGAAACGAAGTTGCTGCGGTGGTTCAACAACGTCT
GGCAGCAGGTAAGCGTGCCCCCGGTCTGGCGGTTGTCTTGGTCGGTGAAAACCCGGCGTCACAGATCTATGTGGCAAGTA
AGCGTAAGGCTTGTGAAGAGGTGGGCTTTGTCTCACGCTCTTATGATCTGCCAATGGCGACCAGTGAAGCAGAGCTACTG
GCCCTGATTGATTCATTGAATGAAGATACCGAAATCGACGGCATTCTAATACAACTGCCACTGCCTAACGGGATTGATAA
CGTTAAAGTTCTGGAGCGTATTCACCCTGATAAGGATGTGGATGGTTTCCACCCATATAACGTGGGCCGCCTGTGCCAGC
GTGCGCCAAAACTGCGCGCTTGTACCCCCCGTGGCATCATGACGCTGTTAGAACGTTATGACATCCCAACTTACGGCCTG
AACGCGGTGGTCGTCGGCGCATCCAATATTGTTGGTCGCCCGATGAGTCTTGAATTGCTGCTGGCCGGTTGCACCACCAC
GGTTACCCATCGCTTTACTAAGAATCTGCGTCACCATATTGAGAATGCCGACCTGCTGGTGGTCGCTGTCGGTAAACCGG
GCTTTATTCCCGGAGAATGGATTAAACCGGGCGCTATCGTGATTGATGTGGGTATTAACCGTCTGGAAAGTGGTAAAGTA
GTCGGCGATGTAGCATTTGATGTCGCTGCTGAACGTGCGGGTTGGATTACACCGGTTCCAGGTGGCGTCGGGCCAATGAC
CGTTGCAACACTGATACAAAATACCTTGCAAGCCTGCGAGGAATATCACGACATCAGCCAAAATTGA

Upstream 100 bases:

>100_bases
TTAATCGTTAATTAATCGTCATTATGCGCCTGACAGCGCGGCCCGCTTCTGACAAAATAGGCCGATTCCCGTTTTTTTCA
ATTGATGGACCCCTCCACTG

Downstream 100 bases:

>100_bases
GTTAAAGGACAGTAATGGACATTTTTCATTTGGAAAAACACCCCCATGTCGAGCTATGTGACCTACTAAAGTTACAAGGT
TGGAATGACAGCGGTGCATC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MSAKIIDGKTIAQQVRNEVAAVVQQRLAAGKRAPGLAVVLVGENPASQIYVASKRKACEEVGFVSRSYDLPMATSEAELL
ALIDSLNEDTEIDGILIQLPLPNGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPKLRACTPRGIMTLLERYDIPTYGL
NAVVVGASNIVGRPMSLELLLAGCTTTVTHRFTKNLRHHIENADLLVVAVGKPGFIPGEWIKPGAIVIDVGINRLESGKV
VGDVAFDVAAERAGWITPVPGGVGPMTVATLIQNTLQACEEYHDISQN

Sequences:

>Translated_288_residues
MSAKIIDGKTIAQQVRNEVAAVVQQRLAAGKRAPGLAVVLVGENPASQIYVASKRKACEEVGFVSRSYDLPMATSEAELL
ALIDSLNEDTEIDGILIQLPLPNGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPKLRACTPRGIMTLLERYDIPTYGL
NAVVVGASNIVGRPMSLELLLAGCTTTVTHRFTKNLRHHIENADLLVVAVGKPGFIPGEWIKPGAIVIDVGINRLESGKV
VGDVAFDVAAERAGWITPVPGGVGPMTVATLIQNTLQACEEYHDISQN
>Mature_287_residues
SAKIIDGKTIAQQVRNEVAAVVQQRLAAGKRAPGLAVVLVGENPASQIYVASKRKACEEVGFVSRSYDLPMATSEAELLA
LIDSLNEDTEIDGILIQLPLPNGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPKLRACTPRGIMTLLERYDIPTYGLN
AVVVGASNIVGRPMSLELLLAGCTTTVTHRFTKNLRHHIENADLLVVAVGKPGFIPGEWIKPGAIVIDVGINRLESGKVV
GDVAFDVAAERAGWITPVPGGVGPMTVATLIQNTLQACEEYHDISQN

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=45.2380952380952, Blast_Score=231, Evalue=7e-61,
Organism=Homo sapiens, GI222136639, Length=291, Percent_Identity=43.9862542955326, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=42.1768707482993, Blast_Score=221, Evalue=5e-58,
Organism=Homo sapiens, GI36796743, Length=229, Percent_Identity=31.0043668122271, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1786741, Length=284, Percent_Identity=86.6197183098592, Blast_Score=508, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI17568735, Length=290, Percent_Identity=40.6896551724138, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=42.9530201342282, Blast_Score=227, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6319558, Length=285, Percent_Identity=42.4561403508772, Blast_Score=214, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6322933, Length=319, Percent_Identity=25.3918495297806, Blast_Score=79, Evalue=6e-16,
Organism=Drosophila melanogaster, GI17136816, Length=297, Percent_Identity=48.1481481481481, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI17136818, Length=297, Percent_Identity=48.1481481481481, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24645718, Length=293, Percent_Identity=41.9795221843003, Blast_Score=206, Evalue=1e-53,
Organism=Drosophila melanogaster, GI17137370, Length=293, Percent_Identity=41.9795221843003, Blast_Score=206, Evalue=1e-53,
Organism=Drosophila melanogaster, GI62472483, Length=293, Percent_Identity=41.9795221843003, Blast_Score=206, Evalue=1e-53,
Organism=Drosophila melanogaster, GI45551871, Length=293, Percent_Identity=41.9795221843003, Blast_Score=206, Evalue=1e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30983; Mature: 30852

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKIIDGKTIAQQVRNEVAAVVQQRLAAGKRAPGLAVVLVGENPASQIYVASKRKACEE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEHHHHHHHHH
VGFVSRSYDLPMATSEAELLALIDSLNEDTEIDGILIQLPLPNGIDNVKVLERIHPDKDV
HCCHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCC
DGFHPYNVGRLCQRAPKLRACTPRGIMTLLERYDIPTYGLNAVVVGASNIVGRPMSLELL
CCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECHHHHCCCCEEEEE
LAGCTTTVTHRFTKNLRHHIENADLLVVAVGKPGFIPGEWIKPGAIVIDVGINRLESGKV
EECHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCE
VGDVAFDVAAERAGWITPVPGGVGPMTVATLIQNTLQACEEYHDISQN
EEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SAKIIDGKTIAQQVRNEVAAVVQQRLAAGKRAPGLAVVLVGENPASQIYVASKRKACEE
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEHHHHHHHHH
VGFVSRSYDLPMATSEAELLALIDSLNEDTEIDGILIQLPLPNGIDNVKVLERIHPDKDV
HCCHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCC
DGFHPYNVGRLCQRAPKLRACTPRGIMTLLERYDIPTYGLNAVVVGASNIVGRPMSLELL
CCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECHHHHCCCCEEEEE
LAGCTTTVTHRFTKNLRHHIENADLLVVAVGKPGFIPGEWIKPGAIVIDVGINRLESGKV
EECHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCE
VGDVAFDVAAERAGWITPVPGGVGPMTVATLIQNTLQACEEYHDISQN
EEHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA