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The map label for this gene is mepA [H]

Identifier: 218929824

GI number: 218929824

Start: 3084827

End: 3085654

Strand: Direct

Name: mepA [H]

Synonym: YPO2752

Alternate gene names: 218929824

Gene position: 3084827-3085654 (Clockwise)

Preceding gene: 218929823

Following gene: 218929825

Centisome position: 66.29

GC content: 52.9

Gene sequence:

>828_bases
ATGAAAAATTGGATAGTGGGTATGGTGGCATTGGTCACCATGGTCCCTGTCATGGCGGCCACGCCATGGCAAAAGATTAC
GCACCCGGTGGCGGGTTCTCCACAGTCAATCGGTGGCTTTGCTAATGGTTGCGTGATTGGCGCGCAGCCTTTGCCGCTCG
AGTCTGCAGACTATCAAGTGATGCGTTCGGATCAGCGCCGTTATTTTGGTCACCCTGATTTACTGAATTTTATTCACCGT
CTCAGTGCACAAACTCATCAACAACAATTGGGCACGGTGTTGATTGGTGATATGGCGATGCCTGCAGGCGGGCGTTTTAG
CAGTGGGCATGCCAGCCACCAGTCTGGTTTGGATGTGGATATTTGGCTACAACTGCCAAAGCAGCGTTGGAGCCAGCAGC
AGTTATTGAAACCGCAGCCGATAGACTTGGTCGCGGTTGATGGCAAGCGGGTGATCCCCGCACTTTGGCAGCCACAAATT
GAGAGTTTGATCAAATTGGCCGCCAAAGATAACGATGTCACGCGTATCTTCGTCAATCCGGCAATTAAAAAGCAGCTTTG
TCTGGATGCAGGTGCCGATCGTCAATGGCTGCATAAAGTCCGGCCGTGGTTTGCTCATCGTGCACACATGCATGTGCGCT
TACGTTGTCCGGCCAATAGCCTAGAGTGTGTGGACCAAGACACTCCACCGCCGGGCGATGGCTGTGGTGCTGAGCTGGAA
AGCTGGTTCCAACCGCCTCCGCCAAGTGCTAAACCAGGTAAAACCTTGCCACCGCCATTACCGCCTTCTTGTCAGGCTTT
ACTGGATAACCACTTCGCTACGGAATAA

Upstream 100 bases:

>100_bases
TTGCAGAAGCGATGATGGCTATCGTACTTATGGATCACCTACTGCGCCAGCGCGCGCAGTGCGGCGATGTGGCTTCAGAC
GTTCCGCGCTGGTAGATAGC

Downstream 100 bases:

>100_bases
GTCATGGATTGGTTTACCCTTGGTCCTGCCGTTTTGGGGATACTCTTTGCCGTTGCATTATTAGCCGGGTTCATTGATTC
CATTGCCGGCGGGGGCGGCT

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: D-alanyl-D-alanine-endopeptidase; DD-endopeptidase [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKNWIVGMVALVTMVPVMAATPWQKITHPVAGSPQSIGGFANGCVIGAQPLPLESADYQVMRSDQRRYFGHPDLLNFIHR
LSAQTHQQQLGTVLIGDMAMPAGGRFSSGHASHQSGLDVDIWLQLPKQRWSQQQLLKPQPIDLVAVDGKRVIPALWQPQI
ESLIKLAAKDNDVTRIFVNPAIKKQLCLDAGADRQWLHKVRPWFAHRAHMHVRLRCPANSLECVDQDTPPPGDGCGAELE
SWFQPPPPSAKPGKTLPPPLPPSCQALLDNHFATE

Sequences:

>Translated_275_residues
MKNWIVGMVALVTMVPVMAATPWQKITHPVAGSPQSIGGFANGCVIGAQPLPLESADYQVMRSDQRRYFGHPDLLNFIHR
LSAQTHQQQLGTVLIGDMAMPAGGRFSSGHASHQSGLDVDIWLQLPKQRWSQQQLLKPQPIDLVAVDGKRVIPALWQPQI
ESLIKLAAKDNDVTRIFVNPAIKKQLCLDAGADRQWLHKVRPWFAHRAHMHVRLRCPANSLECVDQDTPPPGDGCGAELE
SWFQPPPPSAKPGKTLPPPLPPSCQALLDNHFATE
>Mature_275_residues
MKNWIVGMVALVTMVPVMAATPWQKITHPVAGSPQSIGGFANGCVIGAQPLPLESADYQVMRSDQRRYFGHPDLLNFIHR
LSAQTHQQQLGTVLIGDMAMPAGGRFSSGHASHQSGLDVDIWLQLPKQRWSQQQLLKPQPIDLVAVDGKRVIPALWQPQI
ESLIKLAAKDNDVTRIFVNPAIKKQLCLDAGADRQWLHKVRPWFAHRAHMHVRLRCPANSLECVDQDTPPPGDGCGAELE
SWFQPPPPSAKPGKTLPPPLPPSCQALLDNHFATE

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=252, Percent_Identity=73.4126984126984, Blast_Score=392, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 30323; Mature: 30323

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNWIVGMVALVTMVPVMAATPWQKITHPVAGSPQSIGGFANGCVIGAQPLPLESADYQV
CCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHCCCCCCCEEECCCCCCCCCCCHHH
MRSDQRRYFGHPDLLNFIHRLSAQTHQQQLGTVLIGDMAMPAGGRFSSGHASHQSGLDVD
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCCCCCCEE
IWLQLPKQRWSQQQLLKPQPIDLVAVDGKRVIPALWQPQIESLIKLAAKDNDVTRIFVNP
EEEECCHHHCCHHHCCCCCCCEEEEECCCCCCHHHCCHHHHHHHHHHHCCCCEEEEEECH
AIKKQLCLDAGADRQWLHKVRPWFAHRAHMHVRLRCPANSLECVDQDTPPPGDGCGAELE
HHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCHHHCCCCCCCCCCCCCHHHH
SWFQPPPPSAKPGKTLPPPLPPSCQALLDNHFATE
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKNWIVGMVALVTMVPVMAATPWQKITHPVAGSPQSIGGFANGCVIGAQPLPLESADYQV
CCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHCCCCCCCEEECCCCCCCCCCCHHH
MRSDQRRYFGHPDLLNFIHRLSAQTHQQQLGTVLIGDMAMPAGGRFSSGHASHQSGLDVD
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCCCCCCCCEE
IWLQLPKQRWSQQQLLKPQPIDLVAVDGKRVIPALWQPQIESLIKLAAKDNDVTRIFVNP
EEEECCHHHCCHHHCCCCCCCEEEEECCCCCCHHHCCHHHHHHHHHHHCCCCEEEEEECH
AIKKQLCLDAGADRQWLHKVRPWFAHRAHMHVRLRCPANSLECVDQDTPPPGDGCGAELE
HHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCHHHCCCCCCCCCCCCCHHHH
SWFQPPPPSAKPGKTLPPPLPPSCQALLDNHFATE
HHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA