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The map label for this gene is miaB [H]

Identifier: 218929701

GI number: 218929701

Start: 2942788

End: 2944212

Strand: Reverse

Name: miaB [H]

Synonym: YPO2620

Alternate gene names: 218929701

Gene position: 2944212-2942788 (Counterclockwise)

Preceding gene: 218929704

Following gene: 218929700

Centisome position: 63.27

GC content: 48.21

Gene sequence:

>1425_bases
ATGACTAAAAAACTGCATATCAAAACCTGGGGTTGCCAGATGAATGAATACGATTCATCTAAAATGGCAGATCTCTTGGC
CAGTACCCATGGCTACCAGTTAACCACCATCCCGGAAGAAGCGGATTTACTGCTGCTAAATACCTGTTCTATCCGGGAAA
AAGCACAGGAAAAAGTATTCAGCCTGCTAGGGCAATGGAAATTATTGAAAGAGAAAAACCCGCAGTTGATTATTGGGGTC
GGTGGTTGCGTGGCGTCACAGGAAGGTGAACAATTGCGCCAGCGCGCCCCCTGTGTCGATGTCATCTTCGGGCCACAAAC
GCTGCATCGTTTACCGGAAATGATTAACCACGTGCAAGGAACGAATAGCCCTGTCGTGGATATCAGTTTTCCCGAAATTG
AAAAATTTGACCGTTTGCCAGAACCACGGGCTGAAGGCCCAACTGCGTTTGTTTCGATCATGGAAGGCTGCAATAAATAT
TGTACATTCTGTGTCGTACCCTACACCCGAGGCGAGGAAGTCAGCCGTCCAAGCGATGACATTCTTTTTGAAATCGCCCA
GTTAGCCGCTCAAGGCGTACGTGAAGTCAACTTACTCGGCCAGAATGTTAACGCCTACCGCGGGGCAACCTACGATGGCG
ATATCTGTAGTTTTGCCGAGCTGTTGCGTTTGGTTGCGGCGATTGATGGTATCGATAGGGTACGATTTACCACCAGCCAC
CCCATTGAGTTTACTGACGATATCATTGATGTTTATCGCGATACGCCTGAATTGGTGAGCTTCCTGCATTTGCCGGTACA
AAGCGGTTCTGACCGTATTTTGACCATGATGAAGCGTGCCCATACCGCCTTGGAATACAAAGCCATCATCCGCAAACTGC
GTCAAGCACGCCCTGATATCCAGATAAGTTCTGATTTTATTGTCGGCTTCCCTGGTGAAACCCAGCAGGATTTTGAACAG
ACCATGAAACTGGTCGCAGATATTCATTTTGATACCAGCTACAGCTTTATTTACTCCCCGCGTCCCGGCACACCAGCGGC
TGATTTACCCAACAATGTGTCCGAAGAGGAGAAAAAACAGCGGCTACATATCCTGCAACAACGCATTAGCCAACAGGCGA
TGGAAATTAGCCGTAAGATGGTAGGGACAGTACAGCGTGTTTTGGTGGAGGGTACCTCTCGCAAAAATGTCATGGAACTG
GCCGGGCGTACGGAAAACAACCGCGTCGTGAACTTTGAGGGTTCACCCGACATGATTGGTAAATTCGTTGATGTCGAAAT
TGTTAACGTTTATGCCAGTTCACTGCGCGGCATTTTACTGCGTACAGAAGATCAGATGGACCTGCGAACCCACGAGTCAC
CGCAATCGGTGATTGCTCGCACCCGTAAAGAAAATGAAATTGGTGTAGGTATTTATCAGCCTTAA

Upstream 100 bases:

>100_bases
CCCTAACGACGAAGCCTTTGCGACTCTCTACTACAACGGAGATTATCTACCACAACGGAGTACCGCACGGAACGAGGTGA
TTTTGATGATGAATAACCCG

Downstream 100 bases:

>100_bases
CCAACATCCATTTTGGTAACGCCCGGCCTAACATCATTGATCTGACAGGTTTAGTTGATCCCGCCCGCGTAACGAGGCAC
TGCATTGTCAGTGCCTGTTT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTKKLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLLLNTCSIREKAQEKVFSLLGQWKLLKEKNPQLIIGV
GGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQGTNSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
CTFCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGIDRVRFTTSH
PIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDIQISSDFIVGFPGETQQDFEQ
TMKLVADIHFDTSYSFIYSPRPGTPAADLPNNVSEEEKKQRLHILQQRISQQAMEISRKMVGTVQRVLVEGTSRKNVMEL
AGRTENNRVVNFEGSPDMIGKFVDVEIVNVYASSLRGILLRTEDQMDLRTHESPQSVIARTRKENEIGVGIYQP

Sequences:

>Translated_474_residues
MTKKLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLLLNTCSIREKAQEKVFSLLGQWKLLKEKNPQLIIGV
GGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQGTNSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
CTFCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGIDRVRFTTSH
PIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDIQISSDFIVGFPGETQQDFEQ
TMKLVADIHFDTSYSFIYSPRPGTPAADLPNNVSEEEKKQRLHILQQRISQQAMEISRKMVGTVQRVLVEGTSRKNVMEL
AGRTENNRVVNFEGSPDMIGKFVDVEIVNVYASSLRGILLRTEDQMDLRTHESPQSVIARTRKENEIGVGIYQP
>Mature_473_residues
TKKLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLLLNTCSIREKAQEKVFSLLGQWKLLKEKNPQLIIGVG
GCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQGTNSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYC
TFCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGIDRVRFTTSHP
IEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDIQISSDFIVGFPGETQQDFEQT
MKLVADIHFDTSYSFIYSPRPGTPAADLPNNVSEEEKKQRLHILQQRISQQAMEISRKMVGTVQRVLVEGTSRKNVMELA
GRTENNRVVNFEGSPDMIGKFVDVEIVNVYASSLRGILLRTEDQMDLRTHESPQSVIARTRKENEIGVGIYQP

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=482, Percent_Identity=35.6846473029046, Blast_Score=282, Evalue=4e-76,
Organism=Homo sapiens, GI28872782, Length=482, Percent_Identity=35.6846473029046, Blast_Score=281, Evalue=7e-76,
Organism=Homo sapiens, GI93277076, Length=443, Percent_Identity=29.7968397291196, Blast_Score=177, Evalue=1e-44,
Organism=Escherichia coli, GI1786882, Length=474, Percent_Identity=83.7552742616034, Blast_Score=840, Evalue=0.0,
Organism=Escherichia coli, GI1787057, Length=404, Percent_Identity=30.1980198019802, Blast_Score=172, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17553146, Length=463, Percent_Identity=32.3974082073434, Blast_Score=236, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI71996771, Length=256, Percent_Identity=31.25, Blast_Score=114, Evalue=8e-26,
Organism=Drosophila melanogaster, GI21356207, Length=437, Percent_Identity=36.3844393592677, Blast_Score=284, Evalue=1e-76,
Organism=Drosophila melanogaster, GI19922432, Length=470, Percent_Identity=29.7872340425532, Blast_Score=169, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 53406; Mature: 53275

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLLLNTCSIREKAQEKVF
CCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCEEEEECHHHHHHHHHHHH
SLLGQWKLLKEKNPQLIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQG
HHHHHHHHHHCCCCCEEEECCCHHCCCHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHCC
TNSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDD
CCCCEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCHH
ILFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGIDRVRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCHHEEEECCC
PIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDI
CCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QISSDFIVGFPGETQQDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPNNVSEEEKKQ
EECCCEEEECCCCCHHHHHHHHHHHHHHEECCCCEEEECCCCCCCCCCCCCCCCHHHHHH
RLHILQQRISQQAMEISRKMVGTVQRVLVEGTSRKNVMELAGRTENNRVVNFEGSPDMIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEECCCHHHHH
KFVDVEIVNVYASSLRGILLRTEDQMDLRTHESPQSVIARTRKENEIGVGIYQP
HHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TKKLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLLLNTCSIREKAQEKVF
CCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCEEEEECHHHHHHHHHHHH
SLLGQWKLLKEKNPQLIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQG
HHHHHHHHHHCCCCCEEEECCCHHCCCHHHHHHHHCCEEEEEECHHHHHHHHHHHHHHCC
TNSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDD
CCCCEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCHH
ILFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGIDRVRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHHHHCCCHHEEEECCC
PIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDI
CCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
QISSDFIVGFPGETQQDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPNNVSEEEKKQ
EECCCEEEECCCCCHHHHHHHHHHHHHHEECCCCEEEECCCCCCCCCCCCCCCCHHHHHH
RLHILQQRISQQAMEISRKMVGTVQRVLVEGTSRKNVMELAGRTENNRVVNFEGSPDMIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEECCCHHHHH
KFVDVEIVNVYASSLRGILLRTEDQMDLRTHESPQSVIARTRKENEIGVGIYQP
HHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA