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The map label for this gene is asnB [H]

Identifier: 218929704

GI number: 218929704

Start: 2946948

End: 2948612

Strand: Reverse

Name: asnB [H]

Synonym: YPO2623

Alternate gene names: 218929704

Gene position: 2948612-2946948 (Counterclockwise)

Preceding gene: 218929705

Following gene: 218929701

Centisome position: 63.36

GC content: 49.19

Gene sequence:

>1665_bases
ATGTGTTCTATTTTCGGGGTCTTAGAGCTTAAAACCGACCCTATTGAATTACGCAAGAAAGCGCTAGAAATGTCACGTTT
GATGCGTCACCGTGGCCCAGACTGGTCTGGTGTCTGGGCGAATGATAAAGCCATCCTCGCCCATGAGCGCTTATCTATTG
TTGACGTCAATACCGGTGCCCAGCCGCTGTATAATGCCGCCCACACACATATCTTGGCCGTGAATGGTGAAATTTATAAT
CATCAAGCATTACGTCAGCAATATAGCGACCGTTACGCCTTCCAAACGGGTTCAGACTGTGAAGTTATTCTGGCGTTGTA
TCAAGAAAAAGGGCCCGCGTTCCTCGATGATCTGCAAGGGATGTTTGCCTTCGTCCTATACGATACCGAGAAAGACGCCT
ACCTGATCGGCCGTGACCATTTAGGTATTATCCCGCTGTATATGGGGCACGACGAACACGGCAATATGTTTGTAGCTTCG
GAAATGAAAGCCTTGGTACCCGTTTGCCGCACCATAAAAGAATTCCCTGCTGGCAGCTATTTGTGGAGTCAGGATGGCGA
GATCCGCGAATACTATCATCGCGATTGGTTTGATTACGACCACGTCAAAGACAACGTAACCGACAAAATCCAACTTGCCA
ACGCATTGGAAGATGCGGTTAAAAGCCATCTGATGTCCGATGTCCCTTATGGGGTATTGCTGTCTGGTGGCCTGGATTCA
TCCGTTATCTCTGCTATCACCAAAAAATTTGCCGCCCGCCGTGTAGAAGATAATGAACGCAGCGAAGCCTGGTGGCCACA
ACTGCACTCCTTCGCCGTCGGTTTGATTGGCTCACCCGATTTACGCGCAGCACAAGAAGTCGCTAACCACTTGGGCACGG
TACACCATGAAATTCATTTCACCGTGCAGGAAGGGCTGGATGCCATCCGCGATGTGATTTATCACATCGAAACCTATGAT
GTGACGACTATCCGCGCGTCAACGCCGATGTACCTGATGTCACGTAAAATCAAAGCCATGGGCATCAAAATGGTGCTATC
TGGTGAGGGTTCTGACGAAGTCTTCGGTGGGTATCTCTATTTCCACAAAGCGCCCAACGCCAAAGAATTACATGAAGAAA
CCGTGCGTAAACTGTTAGCTCTCCATATGTATGACTGCGCCCGTGCCAACAAAGCGATGTCAGCTTGGGGCGTTGAAGCT
CGTGTGCCCTTCCTGGATAAAAACTTCCTTGATGTCGCCATGCGTATCAATCCACAGGATAAAATGTGCGGCAATGGCAA
AATGGAGAAACATATCCTGCGAGAATGTTTCGAATCTTACCTGCCACACAGCGTCGCCTGGCGTCAGAAAGAACAATTCT
CTGATGGTGTTGGCTATAGCTGGATTGACACCTTGAAAGAGAAAGCAGCCGAGCAAGTCACTGACCAACAGATGGAAACC
GCTCACTTCCGCTTCCCGTACAACACACCTGGCTCGAAAGAAGGGTATTTGTACCGTGAAATCTTTGAAGAGCTGTTCCC
GCTACCTAGTGCGGCGGAATGTGTCCCTGGCGGCCCCTCTGTTGCCTGTTCATCAGCTAAAGCCATTGAATGGGATGAAT
CATTCAAAAATATGGATGATCCATCAGGCCGTGCGGTTGGCGTCCATCAGGATGCTTACAAATAA

Upstream 100 bases:

>100_bases
GCTTGCATCAATCGATAGCAAACGCCCAAAAAGCCCCTGGCGATAAATAACGCAATGTCAAGATCATCACCGCCAAAGCA
GAGACATTAGGGAGATTATT

Downstream 100 bases:

>100_bases
TTGAACACGATAAGCGGGGTTAATCCCCCGCGCCGACTGCTGACAAAGTTAAATGAAGGAGGCACGTGCCGCTGGGGTCA
TAGTAACCCTCTTGCTTATC

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MCSIFGVLELKTDPIELRKKALEMSRLMRHRGPDWSGVWANDKAILAHERLSIVDVNTGAQPLYNAAHTHILAVNGEIYN
HQALRQQYSDRYAFQTGSDCEVILALYQEKGPAFLDDLQGMFAFVLYDTEKDAYLIGRDHLGIIPLYMGHDEHGNMFVAS
EMKALVPVCRTIKEFPAGSYLWSQDGEIREYYHRDWFDYDHVKDNVTDKIQLANALEDAVKSHLMSDVPYGVLLSGGLDS
SVISAITKKFAARRVEDNERSEAWWPQLHSFAVGLIGSPDLRAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYD
VTTIRASTPMYLMSRKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKAMSAWGVEA
RVPFLDKNFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPHSVAWRQKEQFSDGVGYSWIDTLKEKAAEQVTDQQMET
AHFRFPYNTPGSKEGYLYREIFEELFPLPSAAECVPGGPSVACSSAKAIEWDESFKNMDDPSGRAVGVHQDAYK

Sequences:

>Translated_554_residues
MCSIFGVLELKTDPIELRKKALEMSRLMRHRGPDWSGVWANDKAILAHERLSIVDVNTGAQPLYNAAHTHILAVNGEIYN
HQALRQQYSDRYAFQTGSDCEVILALYQEKGPAFLDDLQGMFAFVLYDTEKDAYLIGRDHLGIIPLYMGHDEHGNMFVAS
EMKALVPVCRTIKEFPAGSYLWSQDGEIREYYHRDWFDYDHVKDNVTDKIQLANALEDAVKSHLMSDVPYGVLLSGGLDS
SVISAITKKFAARRVEDNERSEAWWPQLHSFAVGLIGSPDLRAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYD
VTTIRASTPMYLMSRKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKAMSAWGVEA
RVPFLDKNFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPHSVAWRQKEQFSDGVGYSWIDTLKEKAAEQVTDQQMET
AHFRFPYNTPGSKEGYLYREIFEELFPLPSAAECVPGGPSVACSSAKAIEWDESFKNMDDPSGRAVGVHQDAYK
>Mature_554_residues
MCSIFGVLELKTDPIELRKKALEMSRLMRHRGPDWSGVWANDKAILAHERLSIVDVNTGAQPLYNAAHTHILAVNGEIYN
HQALRQQYSDRYAFQTGSDCEVILALYQEKGPAFLDDLQGMFAFVLYDTEKDAYLIGRDHLGIIPLYMGHDEHGNMFVAS
EMKALVPVCRTIKEFPAGSYLWSQDGEIREYYHRDWFDYDHVKDNVTDKIQLANALEDAVKSHLMSDVPYGVLLSGGLDS
SVISAITKKFAARRVEDNERSEAWWPQLHSFAVGLIGSPDLRAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYD
VTTIRASTPMYLMSRKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKAMSAWGVEA
RVPFLDKNFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPHSVAWRQKEQFSDGVGYSWIDTLKEKAAEQVTDQQMET
AHFRFPYNTPGSKEGYLYREIFEELFPLPSAAECVPGGPSVACSSAKAIEWDESFKNMDDPSGRAVGVHQDAYK

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI296010848, Length=560, Percent_Identity=37.5, Blast_Score=314, Evalue=2e-85,
Organism=Homo sapiens, GI168229248, Length=560, Percent_Identity=37.1428571428571, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI168229252, Length=560, Percent_Identity=37.1428571428571, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI168229250, Length=560, Percent_Identity=37.1428571428571, Blast_Score=313, Evalue=2e-85,
Organism=Homo sapiens, GI296010852, Length=494, Percent_Identity=38.0566801619433, Blast_Score=283, Evalue=4e-76,
Organism=Homo sapiens, GI296010850, Length=494, Percent_Identity=38.0566801619433, Blast_Score=283, Evalue=4e-76,
Organism=Escherichia coli, GI1786889, Length=554, Percent_Identity=90.7942238267148, Blast_Score=1068, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147557, Length=568, Percent_Identity=42.2535211267606, Blast_Score=425, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71993933, Length=559, Percent_Identity=42.2182468694097, Blast_Score=417, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17560178, Length=490, Percent_Identity=34.6938775510204, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI25147560, Length=311, Percent_Identity=38.5852090032154, Blast_Score=188, Evalue=8e-48,
Organism=Saccharomyces cerevisiae, GI6321563, Length=585, Percent_Identity=47.5213675213675, Blast_Score=520, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6325403, Length=586, Percent_Identity=45.9044368600683, Blast_Score=508, Evalue=1e-144,
Organism=Drosophila melanogaster, GI45553209, Length=576, Percent_Identity=44.7916666666667, Blast_Score=469, Evalue=1e-132,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 62736; Mature: 62736

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSIFGVLELKTDPIELRKKALEMSRLMRHRGPDWSGVWANDKAILAHERLSIVDVNTGA
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEEEEECCCC
QPLYNAAHTHILAVNGEIYNHQALRQQYSDRYAFQTGSDCEVILALYQEKGPAFLDDLQG
CHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHH
MFAFVLYDTEKDAYLIGRDHLGIIPLYMGHDEHGNMFVASEMKALVPVCRTIKEFPAGSY
HEEEEEEECCCCEEEEECCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCE
LWSQDGEIREYYHRDWFDYDHVKDNVTDKIQLANALEDAVKSHLMSDVPYGVLLSGGLDS
EECCCCHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCH
SVISAITKKFAARRVEDNERSEAWWPQLHSFAVGLIGSPDLRAAQEVANHLGTVHHEIHF
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHEEE
TVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMSRKIKAMGIKMVLSGEGSDEVFGGYLY
EHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCEEEEEECCCCCCCEEEEEE
FHKAPNAKELHEETVRKLLALHMYDCARANKAMSAWGVEARVPFLDKNFLDVAMRINPQD
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCEEEEEEECCCC
KMCGNGKMEKHILRECFESYLPHSVAWRQKEQFSDGVGYSWIDTLKEKAAEQVTDQQMET
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
AHFRFPYNTPGSKEGYLYREIFEELFPLPSAAECVPGGPSVACSSAKAIEWDESFKNMDD
HEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCC
PSGRAVGVHQDAYK
CCCCEEECCCCCCC
>Mature Secondary Structure
MCSIFGVLELKTDPIELRKKALEMSRLMRHRGPDWSGVWANDKAILAHERLSIVDVNTGA
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEEEEECCCC
QPLYNAAHTHILAVNGEIYNHQALRQQYSDRYAFQTGSDCEVILALYQEKGPAFLDDLQG
CHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHH
MFAFVLYDTEKDAYLIGRDHLGIIPLYMGHDEHGNMFVASEMKALVPVCRTIKEFPAGSY
HEEEEEEECCCCEEEEECCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCE
LWSQDGEIREYYHRDWFDYDHVKDNVTDKIQLANALEDAVKSHLMSDVPYGVLLSGGLDS
EECCCCHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCH
SVISAITKKFAARRVEDNERSEAWWPQLHSFAVGLIGSPDLRAAQEVANHLGTVHHEIHF
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHEEE
TVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMSRKIKAMGIKMVLSGEGSDEVFGGYLY
EHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCEEEEEECCCCCCCEEEEEE
FHKAPNAKELHEETVRKLLALHMYDCARANKAMSAWGVEARVPFLDKNFLDVAMRINPQD
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCEEEEEEECCCC
KMCGNGKMEKHILRECFESYLPHSVAWRQKEQFSDGVGYSWIDTLKEKAAEQVTDQQMET
CCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
AHFRFPYNTPGSKEGYLYREIFEELFPLPSAAECVPGGPSVACSSAKAIEWDESFKNMDD
HEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCC
PSGRAVGVHQDAYK
CCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]