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The map label for this gene is leuS [H]

Identifier: 218929691

GI number: 218929691

Start: 2930913

End: 2933495

Strand: Reverse

Name: leuS [H]

Synonym: YPO2610

Alternate gene names: 218929691

Gene position: 2933495-2930913 (Counterclockwise)

Preceding gene: 218929693

Following gene: 218929690

Centisome position: 63.04

GC content: 51.06

Gene sequence:

>2583_bases
ATGCAAGAGCAATACCGCCCAGAAGATATCGAAACGCAAGTACAGCTTCACTGGCAAGAGAAGCAAACCTTTAAAGTCAC
TGAAGACGCCAGCAAAGAAAAATATTATTGCCTATCTATGCTGCCATACCCTTCAGGACGCCTGCATATGGGGCACGTTC
GTAATTACACCATTGGCGACGTTATCTCTCGTTACCAGCGTATGTTGGGCAAAAACGTCTTGCAGCCAATTGGCTGGGAT
GCGTTTGGCCTGCCCGCTGAAGGGGCTGCGGTAAAAAACAATACCGCCCCCGCACCTTGGACTTACGACAACATCGAATA
CATGAAGAACCAGTTGAAATTACTGGGCTTCGGCTATGACTGGGATCGAGAAATTGCCACCTGCAAACCTGATTATTATC
GTTGGGAGCAGTGGTTCTTCACCAAGTTGTATGAAAAAGGAATGGTCTACAAGAAGACCTCTGCGGTTAACTGGTGTCCA
CACGATCTCACTGTGCTGGCTAACGAGCAGGTCATTGACGGCTGCTGCTGGCGTTGTGACACCAAAGTTGAACGTAAAGA
GATCCCGCAGTGGTTTATCAAAATCACCGATTACGCTGAGCAGTTGCTCAACGATCTGGACACGCTGGAAAGCTGGCCAG
AGCAGGTGAAAACCATGCAACGTAACTGGATTGGCCGCTCTGAAGGGGTCGATATCGTTTTCGACGTCGTGGATAGCGAA
GAGAAACTGTCTGTTTATACCACCCGGCCAGACACCTTTATGGGCGTGACCTACGTAGCGGTTGCTGCTGGTCATCCTTT
GTCATTGCAAGCGGCGGCGACTAATCCGGCCCTGGCTGATTTCGTTGCTGAATGCCGTAATACCAAGGTTGCCGAAGCTG
AAATGGCAACCATGGAGAAAAAAGGCATGGCGACGGGTCTGTATGCCATCCATCCATTAACTGGCGAGAAACTGCCTATA
TGGGCCGCTAACTTTGTCTTGATGGATTACGGTACTGGCGCAGTGATGGCCGTTCCGGGCCACGATGCACGTGACTGGGA
ATTTGCCACCAAATATAACCTGCCAATCAAACCGGTTATTTTGGCCGCGGATGGCAGTGAGCCAGACTTGAGTCAGGAAG
CCATGACCGAGAAAGGCACGCTGTTTAACTCTGGTGAGTTTGATGGCCTGAATCATGAAGATGGTTTTAATGCCATTGCC
GATAAACTGGTCGCACTCGGTGTTGGGCAGCGCAAAGTAAACTATCGTCTGCGTGACTGGGGTGTTTCTCGCCAGCGTTA
TTGGGGTGCCCCGATCCCAATGGTTACGTTGGAAGATGGGACTGTCGTTCCTACCCCAGAAGATCAACTGCCGGTTATCC
TGCCAGAAGATGTGGTAATGGACGGTATTTCCAGCCCGATCAAGGCCGATCCTGAGTGGGCTAAAACCACCGTTAACGGT
ATTCCGGGTCTGCGTGAAACCGATACCTTCGATACCTTTATGGAATCATCTTGGTATTACGCGCGCTACACCTGCCCGCA
ATATGATGACGGTATGCTGGACCCCGCAGCCGCTAACTACTGGTTACCTGTCGATCAGTATGTTGGGGGCATTGAACATG
CCATTATGCATCTGATGTATTTCCGCTTCTTCCACAAATTACTGCGTGACGCCGGTCTGGTGGACTCCGATGAACCCGCC
AAACGCCTGTTATGTCAGGGGATGGTACTGGCTGACGCCTTCTATTACACCGGTAATAACGGTGAGCGTATTTGGGTTTC
ACCGGTCGATGCGATTGTCGAACGTGATGACAAAGGCCGTATCGTTAAAGCTGTAGATGCGGAAGGCCATGAGCTGGTGT
ACGCCGGCATGAGTAAAATGTCGAAATCGAAAAACAACGGTATCGACCCGCAAGTCATGGTAGAAAAATACGGCGCGGAT
ACCGTACGTCTGTTTATGATGTTTGCCTCACCAGCAGAAATGACGCTGGAATGGCAGGAGTCGGGCGTTGAAGGGGCTAA
CCGCTTCCTGAAGCGTGTTTGGCGTCTGGCCTTTGATCACACCGCGAAAGGTGCAGTGAAGCCACTGGATATTGCCAGCC
TGACTGAAGAACAGAAATCCCTGCGTCGCGACCTGCATAAAACCATTGCGAAAGTGACCGATGATGTTGGCCGCCGTCAA
ACCTTCAACACGGCAATTGCTGCTGTGATGGAGTTGATGAACAAGCTGGGCCGTGCACCTCAGGAAACGGAGCAAGACCG
CGCACTGATGCAAGAAGCTTTACTGGCGGTAGTCCGGATGCTTTATCCATTCACCCCTCACGTCTGCTTCAGTTTGTGGC
AAGCTTTGGGTGGAGAAGGCGATATCGATACTGCGCCGTGGCCAATTGCTGATGAACAAGCGATGGTTGAAGACTCTAAA
TTAGTGGTCGTACAGGTCAATGGCAAAGTGCGTGGCAGAATTACAGTGCCCGCCGATGCCACTGAACAGCAAGTCCGTGA
ACGCGCTGGTCAGGAGCACTTGGTGGCTAAATATCTGGATGGCGTTACTGTACGTAAAGTCATCTATGTCCCTGGCAAAT
TGCTTAATCTGGTTGTGGGTTAA

Upstream 100 bases:

>100_bases
CCTGCCGCTTATCAGCTATGCTATGCGGATCTCTCTTTATGATATGAAACAGATGATGCTACAGGCGTAGCTGTTTTTCA
CTATAGGACCTCCGGCCGCC

Downstream 100 bases:

>100_bases
GTCAAGGAGGCGTTGTGCGACATCGTATTCTGACGTTGTTGCTGGGGTTGGCGGTGCTGGTCACCGCTGGCTGCGGTTTT
AATCTGCGGGGCACCACTCA

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 860; Mature: 860

Protein sequence:

>860_residues
MQEQYRPEDIETQVQLHWQEKQTFKVTEDASKEKYYCLSMLPYPSGRLHMGHVRNYTIGDVISRYQRMLGKNVLQPIGWD
AFGLPAEGAAVKNNTAPAPWTYDNIEYMKNQLKLLGFGYDWDREIATCKPDYYRWEQWFFTKLYEKGMVYKKTSAVNWCP
HDLTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITDYAEQLLNDLDTLESWPEQVKTMQRNWIGRSEGVDIVFDVVDSE
EKLSVYTTRPDTFMGVTYVAVAAGHPLSLQAAATNPALADFVAECRNTKVAEAEMATMEKKGMATGLYAIHPLTGEKLPI
WAANFVLMDYGTGAVMAVPGHDARDWEFATKYNLPIKPVILAADGSEPDLSQEAMTEKGTLFNSGEFDGLNHEDGFNAIA
DKLVALGVGQRKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVVPTPEDQLPVILPEDVVMDGISSPIKADPEWAKTTVNG
IPGLRETDTFDTFMESSWYYARYTCPQYDDGMLDPAAANYWLPVDQYVGGIEHAIMHLMYFRFFHKLLRDAGLVDSDEPA
KRLLCQGMVLADAFYYTGNNGERIWVSPVDAIVERDDKGRIVKAVDAEGHELVYAGMSKMSKSKNNGIDPQVMVEKYGAD
TVRLFMMFASPAEMTLEWQESGVEGANRFLKRVWRLAFDHTAKGAVKPLDIASLTEEQKSLRRDLHKTIAKVTDDVGRRQ
TFNTAIAAVMELMNKLGRAPQETEQDRALMQEALLAVVRMLYPFTPHVCFSLWQALGGEGDIDTAPWPIADEQAMVEDSK
LVVVQVNGKVRGRITVPADATEQQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLNLVVG

Sequences:

>Translated_860_residues
MQEQYRPEDIETQVQLHWQEKQTFKVTEDASKEKYYCLSMLPYPSGRLHMGHVRNYTIGDVISRYQRMLGKNVLQPIGWD
AFGLPAEGAAVKNNTAPAPWTYDNIEYMKNQLKLLGFGYDWDREIATCKPDYYRWEQWFFTKLYEKGMVYKKTSAVNWCP
HDLTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITDYAEQLLNDLDTLESWPEQVKTMQRNWIGRSEGVDIVFDVVDSE
EKLSVYTTRPDTFMGVTYVAVAAGHPLSLQAAATNPALADFVAECRNTKVAEAEMATMEKKGMATGLYAIHPLTGEKLPI
WAANFVLMDYGTGAVMAVPGHDARDWEFATKYNLPIKPVILAADGSEPDLSQEAMTEKGTLFNSGEFDGLNHEDGFNAIA
DKLVALGVGQRKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVVPTPEDQLPVILPEDVVMDGISSPIKADPEWAKTTVNG
IPGLRETDTFDTFMESSWYYARYTCPQYDDGMLDPAAANYWLPVDQYVGGIEHAIMHLMYFRFFHKLLRDAGLVDSDEPA
KRLLCQGMVLADAFYYTGNNGERIWVSPVDAIVERDDKGRIVKAVDAEGHELVYAGMSKMSKSKNNGIDPQVMVEKYGAD
TVRLFMMFASPAEMTLEWQESGVEGANRFLKRVWRLAFDHTAKGAVKPLDIASLTEEQKSLRRDLHKTIAKVTDDVGRRQ
TFNTAIAAVMELMNKLGRAPQETEQDRALMQEALLAVVRMLYPFTPHVCFSLWQALGGEGDIDTAPWPIADEQAMVEDSK
LVVVQVNGKVRGRITVPADATEQQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLNLVVG
>Mature_860_residues
MQEQYRPEDIETQVQLHWQEKQTFKVTEDASKEKYYCLSMLPYPSGRLHMGHVRNYTIGDVISRYQRMLGKNVLQPIGWD
AFGLPAEGAAVKNNTAPAPWTYDNIEYMKNQLKLLGFGYDWDREIATCKPDYYRWEQWFFTKLYEKGMVYKKTSAVNWCP
HDLTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITDYAEQLLNDLDTLESWPEQVKTMQRNWIGRSEGVDIVFDVVDSE
EKLSVYTTRPDTFMGVTYVAVAAGHPLSLQAAATNPALADFVAECRNTKVAEAEMATMEKKGMATGLYAIHPLTGEKLPI
WAANFVLMDYGTGAVMAVPGHDARDWEFATKYNLPIKPVILAADGSEPDLSQEAMTEKGTLFNSGEFDGLNHEDGFNAIA
DKLVALGVGQRKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVVPTPEDQLPVILPEDVVMDGISSPIKADPEWAKTTVNG
IPGLRETDTFDTFMESSWYYARYTCPQYDDGMLDPAAANYWLPVDQYVGGIEHAIMHLMYFRFFHKLLRDAGLVDSDEPA
KRLLCQGMVLADAFYYTGNNGERIWVSPVDAIVERDDKGRIVKAVDAEGHELVYAGMSKMSKSKNNGIDPQVMVEKYGAD
TVRLFMMFASPAEMTLEWQESGVEGANRFLKRVWRLAFDHTAKGAVKPLDIASLTEEQKSLRRDLHKTIAKVTDDVGRRQ
TFNTAIAAVMELMNKLGRAPQETEQDRALMQEALLAVVRMLYPFTPHVCFSLWQALGGEGDIDTAPWPIADEQAMVEDSK
LVVVQVNGKVRGRITVPADATEQQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLNLVVG

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=873, Percent_Identity=36.31156930126, Blast_Score=518, Evalue=1e-147,
Organism=Homo sapiens, GI5454158, Length=379, Percent_Identity=25.065963060686, Blast_Score=96, Evalue=1e-19,
Organism=Escherichia coli, GI1786861, Length=860, Percent_Identity=84.7674418604651, Blast_Score=1563, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=386, Percent_Identity=25.9067357512953, Blast_Score=116, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71997510, Length=846, Percent_Identity=29.3144208037825, Blast_Score=345, Evalue=8e-95,
Organism=Caenorhabditis elegans, GI71997517, Length=846, Percent_Identity=29.6690307328605, Blast_Score=344, Evalue=9e-95,
Organism=Caenorhabditis elegans, GI212645227, Length=436, Percent_Identity=27.5229357798165, Blast_Score=133, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17510661, Length=393, Percent_Identity=24.4274809160305, Blast_Score=102, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6323414, Length=812, Percent_Identity=35.9605911330049, Blast_Score=503, Evalue=1e-143,
Organism=Saccharomyces cerevisiae, GI6321531, Length=385, Percent_Identity=25.7142857142857, Blast_Score=101, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6325217, Length=151, Percent_Identity=29.8013245033113, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21355409, Length=884, Percent_Identity=33.5972850678733, Blast_Score=437, Evalue=1e-122,
Organism=Drosophila melanogaster, GI281366294, Length=477, Percent_Identity=24.3186582809224, Blast_Score=84, Evalue=6e-16,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 97052; Mature: 97052

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEQYRPEDIETQVQLHWQEKQTFKVTEDASKEKYYCLSMLPYPSGRLHMGHVRNYTIGD
CCCCCCCCCCCEEEEEEEECCCEEEECCCCCCCCEEEEEECCCCCCCEEECCCCCCCHHH
VISRYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTYDNIEYMKNQLKLLGFGYD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHEEEECCCC
WDREIATCKPDYYRWEQWFFTKLYEKGMVYKKTSAVNWCPHDLTVLANEQVIDGCCWRCD
CCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCHHHHHHHHCCC
TKVERKEIPQWFIKITDYAEQLLNDLDTLESWPEQVKTMQRNWIGRSEGVDIVFDVVDSE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCC
EKLSVYTTRPDTFMGVTYVAVAAGHPLSLQAAATNPALADFVAECRNTKVAEAEMATMEK
CCEEEEEECCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHH
KGMATGLYAIHPLTGEKLPIWAANFVLMDYGTGAVMAVPGHDARDWEFATKYNLPIKPVI
CCCCCCEEEEECCCCCCCCEEEEEEEEEECCCCEEEEECCCCCCCCCEEEEECCCCCEEE
LAADGSEPDLSQEAMTEKGTLFNSGEFDGLNHEDGFNAIADKLVALGVGQRKVNYRLRDW
EEECCCCCCCCHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHCEEEHHC
GVSRQRYWGAPIPMVTLEDGTVVPTPEDQLPVILPEDVVMDGISSPIKADPEWAKTTVNG
CCCCHHCCCCCCCEEEECCCEECCCCCCCCCEECCHHHHHHCCCCCCCCCCHHHHHHHCC
IPGLRETDTFDTFMESSWYYARYTCPQYDDGMLDPAAANYWLPVDQYVGGIEHAIMHLMY
CCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCEEHHHHHCHHHHHHHHHHH
FRFFHKLLRDAGLVDSDEPAKRLLCQGMVLADAFYYTGNNGERIWVSPVDAIVERDDKGR
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCEEEECHHHHHHCCCCCCC
IVKAVDAEGHELVYAGMSKMSKSKNNGIDPQVMVEKYGADTVRLFMMFASPAEMTLEWQE
EEEEECCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHCCCCCEEHHHHHCCCCCEEEEHHH
SGVEGANRFLKRVWRLAFDHTAKGAVKPLDIASLTEEQKSLRRDLHKTIAKVTDDVGRRQ
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFNTAIAAVMELMNKLGRAPQETEQDRALMQEALLAVVRMLYPFTPHVCFSLWQALGGEG
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
DIDTAPWPIADEQAMVEDSKLVVVQVNGKVRGRITVPADATEQQVRERAGQEHLVAKYLD
CCCCCCCCCCCCHHHCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHCCHHHHHHHHHC
GVTVRKVIYVPGKLLNLVVG
CCEEEEEEECCHHHHHHHCC
>Mature Secondary Structure
MQEQYRPEDIETQVQLHWQEKQTFKVTEDASKEKYYCLSMLPYPSGRLHMGHVRNYTIGD
CCCCCCCCCCCEEEEEEEECCCEEEECCCCCCCCEEEEEECCCCCCCEEECCCCCCCHHH
VISRYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTYDNIEYMKNQLKLLGFGYD
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHEEEECCCC
WDREIATCKPDYYRWEQWFFTKLYEKGMVYKKTSAVNWCPHDLTVLANEQVIDGCCWRCD
CCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCHHHHHHHHCCC
TKVERKEIPQWFIKITDYAEQLLNDLDTLESWPEQVKTMQRNWIGRSEGVDIVFDVVDSE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCC
EKLSVYTTRPDTFMGVTYVAVAAGHPLSLQAAATNPALADFVAECRNTKVAEAEMATMEK
CCEEEEEECCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHH
KGMATGLYAIHPLTGEKLPIWAANFVLMDYGTGAVMAVPGHDARDWEFATKYNLPIKPVI
CCCCCCEEEEECCCCCCCCEEEEEEEEEECCCCEEEEECCCCCCCCCEEEEECCCCCEEE
LAADGSEPDLSQEAMTEKGTLFNSGEFDGLNHEDGFNAIADKLVALGVGQRKVNYRLRDW
EEECCCCCCCCHHHHHHCCCEECCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHCEEEHHC
GVSRQRYWGAPIPMVTLEDGTVVPTPEDQLPVILPEDVVMDGISSPIKADPEWAKTTVNG
CCCCHHCCCCCCCEEEECCCEECCCCCCCCCEECCHHHHHHCCCCCCCCCCHHHHHHHCC
IPGLRETDTFDTFMESSWYYARYTCPQYDDGMLDPAAANYWLPVDQYVGGIEHAIMHLMY
CCCCCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCEEHHHHHCHHHHHHHHHHH
FRFFHKLLRDAGLVDSDEPAKRLLCQGMVLADAFYYTGNNGERIWVSPVDAIVERDDKGR
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCEEEECHHHHHHCCCCCCC
IVKAVDAEGHELVYAGMSKMSKSKNNGIDPQVMVEKYGADTVRLFMMFASPAEMTLEWQE
EEEEECCCCCEEEEHHHHHHHHCCCCCCCHHHHHHHCCCCCEEHHHHHCCCCCEEEEHHH
SGVEGANRFLKRVWRLAFDHTAKGAVKPLDIASLTEEQKSLRRDLHKTIAKVTDDVGRRQ
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TFNTAIAAVMELMNKLGRAPQETEQDRALMQEALLAVVRMLYPFTPHVCFSLWQALGGEG
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCC
DIDTAPWPIADEQAMVEDSKLVVVQVNGKVRGRITVPADATEQQVRERAGQEHLVAKYLD
CCCCCCCCCCCCHHHCCCCCEEEEEECCCEEEEEECCCCCHHHHHHHHCCHHHHHHHHHC
GVTVRKVIYVPGKLLNLVVG
CCEEEEEEECCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA