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The map label for this gene is hisH

Identifier: 218928686

GI number: 218928686

Start: 1759994

End: 1760584

Strand: Reverse

Name: hisH

Synonym: YPO1545

Alternate gene names: 218928686

Gene position: 1760584-1759994 (Counterclockwise)

Preceding gene: 218928687

Following gene: 218928685

Centisome position: 37.83

GC content: 52.28

Gene sequence:

>591_bases
ATGGATGTGGTGATCCTGGATACCGGTTGTGCCAATCTCTCCTCTGTCACCTATGCGGTACAGCGTTTGGGTTATACACC
GGTTATCAGCCGCGACCCGGCAGTCGTCTTGCGAGCAGACAAACTGTTTATGCCGGGTGTGGGCACCGCCCATGCCGCCA
TGGAACAATTGAGGCAACGCGAGCTTATCGAACTGATCAAAAGTTGTACTCAACCCGTGCTGGGGATCTGCCTTGGTATG
CAGTTGCTGGCAACCAGCAGTGAGGAAAGTGGCGGGATCACCACCCTCGGGATGATTGATGCACCAGTAAAAAAGATGAC
TGATTTTGGTCTGCCGCTGCCGCATATGGGGTGGAATCAGATTACCGCACAAGCCGGTAACCATCTGTTCCGTGGTATCC
CTGACGGAACCTATTTCTACTTCGTGCATGGATATGCAATGCCAATATGCCCAAATACCATCGCCCAAACCAACTACGGC
GAGCCATTTACCGCCGCAGTAGAAAAAGACAACTTCTTTGGCGTGCAGTTTCATCCGGAACGTTCCGGAGCGGCTGGGGC
CCAATTGATGAAAAACTTTCTGGAGATGTAA

Upstream 100 bases:

>100_bases
ATCACCGAGTAGAAAGCCTGTTTAAAGTATTTGGTCGTACCTTGCGTCAAGCCATTCGCGTTGAAGGCAATACCCTGCCA
AGTTCAAAAGGAGTGCTGTA

Downstream 100 bases:

>100_bases
GCACCATGATTATTCCCGCTTTGGATTTGATCGAAGGCAAGGTGGTGCGTTTGCATCAGGGCGATTACGGCCAGCAACGT
GATTACGGCAATCACCCGCT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MDVVILDTGCANLSSVTYAVQRLGYTPVISRDPAVVLRADKLFMPGVGTAHAAMEQLRQRELIELIKSCTQPVLGICLGM
QLLATSSEESGGITTLGMIDAPVKKMTDFGLPLPHMGWNQITAQAGNHLFRGIPDGTYFYFVHGYAMPICPNTIAQTNYG
EPFTAAVEKDNFFGVQFHPERSGAAGAQLMKNFLEM

Sequences:

>Translated_196_residues
MDVVILDTGCANLSSVTYAVQRLGYTPVISRDPAVVLRADKLFMPGVGTAHAAMEQLRQRELIELIKSCTQPVLGICLGM
QLLATSSEESGGITTLGMIDAPVKKMTDFGLPLPHMGWNQITAQAGNHLFRGIPDGTYFYFVHGYAMPICPNTIAQTNYG
EPFTAAVEKDNFFGVQFHPERSGAAGAQLMKNFLEM
>Mature_196_residues
MDVVILDTGCANLSSVTYAVQRLGYTPVISRDPAVVLRADKLFMPGVGTAHAAMEQLRQRELIELIKSCTQPVLGICLGM
QLLATSSEESGGITTLGMIDAPVKKMTDFGLPLPHMGWNQITAQAGNHLFRGIPDGTYFYFVHGYAMPICPNTIAQTNYG
EPFTAAVEKDNFFGVQFHPERSGAAGAQLMKNFLEM

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=196, Percent_Identity=75.5102040816327, Blast_Score=317, Evalue=5e-88,
Organism=Saccharomyces cerevisiae, GI6319725, Length=209, Percent_Identity=33.9712918660287, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_YERPE (Q8ZFX8)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AE0188
- RefSeq:   NP_669928.1
- RefSeq:   NP_992798.1
- RefSeq:   YP_002346561.1
- ProteinModelPortal:   Q8ZFX8
- SMR:   Q8ZFX8
- IntAct:   Q8ZFX8
- GeneID:   1147572
- GeneID:   1174384
- GeneID:   2766320
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO1545
- KEGG:   ypk:y2625
- KEGG:   ypm:YP_1434
- HOGENOM:   HBG292341
- OMA:   SVRFAFE
- ProtClustDB:   PRK13170
- BioCyc:   YPES187410:Y2625-MONOMER
- BioCyc:   YPES214092:YPO1545-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 21302; Mature: 21302

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 77-77 ACT_SITE 178-178 ACT_SITE 180-180

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVVILDTGCANLSSVTYAVQRLGYTPVISRDPAVVLRADKLFMPGVGTAHAAMEQLRQR
CEEEEEECCCCCHHHHHHHHHHCCCCCEECCCCEEEEEECEEECCCCCHHHHHHHHHHHH
ELIELIKSCTQPVLGICLGMQLLATSSEESGGITTLGMIDAPVKKMTDFGLPLPHMGWNQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCCHHH
ITAQAGNHLFRGIPDGTYFYFVHGYAMPICPNTIAQTNYGEPFTAAVEKDNFFGVQFHPE
HHHHHCCHHCCCCCCCEEEEEEECEECCCCCCHHHCCCCCCCEEEEECCCCEEEEEECCC
RSGAAGAQLMKNFLEM
CCCCHHHHHHHHHHCC
>Mature Secondary Structure
MDVVILDTGCANLSSVTYAVQRLGYTPVISRDPAVVLRADKLFMPGVGTAHAAMEQLRQR
CEEEEEECCCCCHHHHHHHHHHCCCCCEECCCCEEEEEECEEECCCCCHHHHHHHHHHHH
ELIELIKSCTQPVLGICLGMQLLATSSEESGGITTLGMIDAPVKKMTDFGLPLPHMGWNQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCCHHH
ITAQAGNHLFRGIPDGTYFYFVHGYAMPICPNTIAQTNYGEPFTAAVEKDNFFGVQFHPE
HHHHHCCHHCCCCCCCEEEEEEECEECCCCCCHHHCCCCCCCEEEEECCCCEEEEEECCC
RSGAAGAQLMKNFLEM
CCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430