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The map label for this gene is hisB

Identifier: 218928687

GI number: 218928687

Start: 1760584

End: 1761651

Strand: Reverse

Name: hisB

Synonym: YPO1546

Alternate gene names: 218928687

Gene position: 1761651-1760584 (Counterclockwise)

Preceding gene: 218928688

Following gene: 218928686

Centisome position: 37.85

GC content: 51.03

Gene sequence:

>1068_bases
ATGAGCCAGAAATTTCTTTTTATTGACCGCGACGGCACCATCATTGCCGAGCCACCAACTGATTATCAGGTTGACCGGTT
GGATAAACTGGCGCTGGAGCCTGATGTCATTCCCGCATTACTGGCGTTGCAAAAAGCAGACTACAAACTGGTGATGATCA
CTAATCAGGATGGCCTCGGCACCAGCAGTTTCCCGCAGGAAACCTTCGATCCGCCACATAACCTGATGATGCAAATCCTG
ACGTCTCAGGGGATCAATTTTGAACAGATACTGATTTGCCCACATCTGCCAGAGGATAACTGCACCTGTCGCAAACCGAA
AACCGCGCTGGTAGAAAGCTATCTGGCAGATGGCGTGATGAACAGCACCAATAGCTATGTCATCGGTGACCGTGAAACTG
ACCTACAACTGGCCGAGAACATGGGCATCAGCGGGTTACGTTATCAGCGTGATGGCTTGAACTGGACGCAAATTGCCAAA
CAACTGACCCAGCGCGACCGCCACGCCTATGTTAATCGCGTGACCAAAGAAACCGCCATTGACGTTAATGTTTGGCTGGA
TCGCGAAGGGGGAAGCAAAATTAAAACCGGCGTGGGCTTCTTCGACCATATGCTGGATCAAATCGCCACCCACGGCGGTT
TTCGCATGGATATTCAGGTCAGCGGCGATCTGTATATCGATGATCACCACACAGTGGAAGATACCGCGCTGGCACTGGGC
GAAGCGATCAACATCGCACTGGGTGACAAACGGGGTATTGGCCGCTTTGGTTTTGTATTGCCGATGGATGAGTGCCTGGC
ACGCTGTGCCTTGGATATTTCTGGTCGCCCGCATTTGGAATACAAAGCTGAATTTAACTACCAGCGTGTCGGCGATCTAA
GCACCGAGATGGTCGAGCACTTCTTCCGCTCCCTTTCGTATGCCATGGCCTGTACCTTGCACCTGAAAACCAAAGGTCGC
AACGATCATCACCGAGTAGAAAGCCTGTTTAAAGTATTTGGTCGTACCTTGCGTCAAGCCATTCGCGTTGAAGGCAATAC
CCTGCCAAGTTCAAAAGGAGTGCTGTAA

Upstream 100 bases:

>100_bases
TTGCCGCCCTTGCCCCCCTGCCCGGCATTGATAACTCAAATAACATTGATAACCAGAATAAAACCTATTCTCAGACCTCC
AGCATCCGTAAGGGAACGAT

Downstream 100 bases:

>100_bases
TGGATGTGGTGATCCTGGATACCGGTTGTGCCAATCTCTCCTCTGTCACCTATGCGGTACAGCGTTTGGGTTATACACCG
GTTATCAGCCGCGACCCGGC

Product: imidazole glycerol-phosphate dehydratase/histidinol phosphatase

Products: NA

Alternate protein names: Histidinol-phosphatase; Imidazoleglycerol-phosphate dehydratase; IGPD

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MSQKFLFIDRDGTIIAEPPTDYQVDRLDKLALEPDVIPALLALQKADYKLVMITNQDGLGTSSFPQETFDPPHNLMMQIL
TSQGINFEQILICPHLPEDNCTCRKPKTALVESYLADGVMNSTNSYVIGDRETDLQLAENMGISGLRYQRDGLNWTQIAK
QLTQRDRHAYVNRVTKETAIDVNVWLDREGGSKIKTGVGFFDHMLDQIATHGGFRMDIQVSGDLYIDDHHTVEDTALALG
EAINIALGDKRGIGRFGFVLPMDECLARCALDISGRPHLEYKAEFNYQRVGDLSTEMVEHFFRSLSYAMACTLHLKTKGR
NDHHRVESLFKVFGRTLRQAIRVEGNTLPSSKGVL

Sequences:

>Translated_355_residues
MSQKFLFIDRDGTIIAEPPTDYQVDRLDKLALEPDVIPALLALQKADYKLVMITNQDGLGTSSFPQETFDPPHNLMMQIL
TSQGINFEQILICPHLPEDNCTCRKPKTALVESYLADGVMNSTNSYVIGDRETDLQLAENMGISGLRYQRDGLNWTQIAK
QLTQRDRHAYVNRVTKETAIDVNVWLDREGGSKIKTGVGFFDHMLDQIATHGGFRMDIQVSGDLYIDDHHTVEDTALALG
EAINIALGDKRGIGRFGFVLPMDECLARCALDISGRPHLEYKAEFNYQRVGDLSTEMVEHFFRSLSYAMACTLHLKTKGR
NDHHRVESLFKVFGRTLRQAIRVEGNTLPSSKGVL
>Mature_354_residues
SQKFLFIDRDGTIIAEPPTDYQVDRLDKLALEPDVIPALLALQKADYKLVMITNQDGLGTSSFPQETFDPPHNLMMQILT
SQGINFEQILICPHLPEDNCTCRKPKTALVESYLADGVMNSTNSYVIGDRETDLQLAENMGISGLRYQRDGLNWTQIAKQ
LTQRDRHAYVNRVTKETAIDVNVWLDREGGSKIKTGVGFFDHMLDQIATHGGFRMDIQVSGDLYIDDHHTVEDTALALGE
AINIALGDKRGIGRFGFVLPMDECLARCALDISGRPHLEYKAEFNYQRVGDLSTEMVEHFFRSLSYAMACTLHLKTKGRN
DHHRVESLFKVFGRTLRQAIRVEGNTLPSSKGVL

Specific function: Histidine biosynthesis; sixth step. Histidine biosynthesis; eighth step. [C]

COG id: COG0131

COG function: function code E; Imidazoleglycerol-phosphate dehydratase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the imidazoleglycerol-phosphate dehydratase family

Homologues:

Organism=Escherichia coli, GI87082027, Length=355, Percent_Identity=81.4084507042254, Blast_Score=613, Evalue=1e-177,
Organism=Escherichia coli, GI1786399, Length=150, Percent_Identity=29.3333333333333, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324776, Length=217, Percent_Identity=39.1705069124424, Blast_Score=156, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS7_YERPE (Q8ZFX7)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AF0188
- RefSeq:   NP_669926.1
- RefSeq:   NP_992799.1
- RefSeq:   YP_002346562.1
- ProteinModelPortal:   Q8ZFX7
- GeneID:   1147570
- GeneID:   1174385
- GeneID:   2766394
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO1546
- KEGG:   ypk:y2623
- KEGG:   ypm:YP_1435
- HOGENOM:   HBG289010
- OMA:   QDGLGTP
- ProtClustDB:   PRK05446
- BioCyc:   YPES187410:Y2623-MONOMER
- BioCyc:   YPES214092:YPO1546-MONOMER
- BRENDA:   3.1.3.15
- BRENDA:   4.2.1.19
- GO:   GO:0005737
- HAMAP:   MF_01022
- InterPro:   IPR023214
- InterPro:   IPR006549
- InterPro:   IPR020566
- InterPro:   IPR005954
- InterPro:   IPR006543
- InterPro:   IPR000807
- InterPro:   IPR020565
- InterPro:   IPR013954
- InterPro:   IPR020568
- Gene3D:   G3DSA:3.40.50.1000
- TIGRFAMs:   TIGR01662
- TIGRFAMs:   TIGR01261
- TIGRFAMs:   TIGR01656

Pfam domain/function: PF00475 IGPD; PF08645 PNK3P; SSF54211 Ribosomal_S5_D2-typ_fold; SSF56784 SSF56784

EC number: =3.1.3.15; =4.2.1.19

Molecular weight: Translated: 39936; Mature: 39805

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00954 IGP_DEHYDRATASE_1; PS00955 IGP_DEHYDRATASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKFLFIDRDGTIIAEPPTDYQVDRLDKLALEPDVIPALLALQKADYKLVMITNQDGLG
CCCCEEEEECCCEEEECCCCCCCHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCCC
TSSFPQETFDPPHNLMMQILTSQGINFEQILICPHLPEDNCTCRKPKTALVESYLADGVM
CCCCCHHHCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
NSTNSYVIGDRETDLQLAENMGISGLRYQRDGLNWTQIAKQLTQRDRHAYVNRVTKETAI
CCCCCEEECCCCCHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEE
DVNVWLDREGGSKIKTGVGFFDHMLDQIATHGGFRMDIQVSGDLYIDDHHTVEDTALALG
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEECCCCCHHHHHHHHH
EAINIALGDKRGIGRFGFVLPMDECLARCALDISGRPHLEYKAEFNYQRVGDLSTEMVEH
HHEEEEECCCCCCCCCEEECCHHHHHHHHHHCCCCCCCEEEEECCCHHHHCCHHHHHHHH
FFRSLSYAMACTLHLKTKGRNDHHRVESLFKVFGRTLRQAIRVEGNTLPSSKGVL
HHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SQKFLFIDRDGTIIAEPPTDYQVDRLDKLALEPDVIPALLALQKADYKLVMITNQDGLG
CCCEEEEECCCEEEECCCCCCCHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCCC
TSSFPQETFDPPHNLMMQILTSQGINFEQILICPHLPEDNCTCRKPKTALVESYLADGVM
CCCCCHHHCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
NSTNSYVIGDRETDLQLAENMGISGLRYQRDGLNWTQIAKQLTQRDRHAYVNRVTKETAI
CCCCCEEECCCCCHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHEE
DVNVWLDREGGSKIKTGVGFFDHMLDQIATHGGFRMDIQVSGDLYIDDHHTVEDTALALG
EEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEECCCCCHHHHHHHHH
EAINIALGDKRGIGRFGFVLPMDECLARCALDISGRPHLEYKAEFNYQRVGDLSTEMVEH
HHEEEEECCCCCCCCCEEECCHHHHHHHHHHCCCCCCCEEEEECCCHHHHCCHHHHHHHH
FFRSLSYAMACTLHLKTKGRNDHHRVESLFKVFGRTLRQAIRVEGNTLPSSKGVL
HHHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430