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The map label for this gene is bioD

Identifier: 218928318

GI number: 218928318

Start: 1300041

End: 1300763

Strand: Direct

Name: bioD

Synonym: YPO1154

Alternate gene names: 218928318

Gene position: 1300041-1300763 (Clockwise)

Preceding gene: 218928317

Following gene: 218928320

Centisome position: 27.94

GC content: 54.22

Gene sequence:

>723_bases
GTGACTAAACGTTGGTTTATCACCGGTACCGATACTGATGTCGGTAAAACCGTCGCCAGTTGTGCATTACTGCAAGCGGC
CACCGCTCAGGGGTACCGTACTGCTGGCTATAAACCGGTAGCATCCGGTAGCCAGATGACCGCTGACGGCTTGCGCAATA
GTGATGCCCTGGCGTTGCAGGCGAATAGCAGCCAGAGGCTCGGTTACTCACAAGTGAATCCGTTCACTTTTTTGGAAGCG
ACATCACCGCATATTGCCAGTGAAAGTGAAGGGAGGGCGATCCCTTTGACGGCGTTATCACAGGGTTTACGTCAACTGGA
ACCCTCCGCTGACTGGATATTGATAGAAGGGGCCGGCGGCTGGTTTACCCCGCTTTCTCCGCAGGCCACATTTGCCGATT
GGGTACAACAGGAACAATTGCCGGTCATTATGGTGGTTGGGGTGAAACTGGGGTGTATCAACCATGCGTTGCTCACGGCA
CAGGCGATTCAGCACGCCGGGCTGACACTGGCCGGTTGGGTTGCCAATGAGGTGACACCGGCAGGGAGACGGCAGGCCGA
ATATCAGGCCACATTGACCCGGATGATAACCGCGCCATTGCTGGGAATTATTCCCTATCTTTCTGATATAGAAGAAAATC
CGGTGACAACACGCCGTGATCTTGGGCACTACTTGGATTTGACGGTATTACGTGCAGCAGAACGGGAGGCTGTCAACATG
TGA

Upstream 100 bases:

>100_bases
TTACAGGGGCGTCAACGTCTGGCGGCGCTACAACGGGCCTATGTCATGCAATCTGGCGGCTATCCACTGAGTTATCATAT
GGTTTATGGGGTTATCTATC

Downstream 100 bases:

>100_bases
TACATATATGGACGCCGCTACAAGCATGTGATGTGGCTTGCAATCAGAGGCCATCAGGTGCGATTAACCTTATGTAGTTA
ATCTACCCGAGTATGGTTAA

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MTKRWFITGTDTDVGKTVASCALLQAATAQGYRTAGYKPVASGSQMTADGLRNSDALALQANSSQRLGYSQVNPFTFLEA
TSPHIASESEGRAIPLTALSQGLRQLEPSADWILIEGAGGWFTPLSPQATFADWVQQEQLPVIMVVGVKLGCINHALLTA
QAIQHAGLTLAGWVANEVTPAGRRQAEYQATLTRMITAPLLGIIPYLSDIEENPVTTRRDLGHYLDLTVLRAAEREAVNM

Sequences:

>Translated_240_residues
MTKRWFITGTDTDVGKTVASCALLQAATAQGYRTAGYKPVASGSQMTADGLRNSDALALQANSSQRLGYSQVNPFTFLEA
TSPHIASESEGRAIPLTALSQGLRQLEPSADWILIEGAGGWFTPLSPQATFADWVQQEQLPVIMVVGVKLGCINHALLTA
QAIQHAGLTLAGWVANEVTPAGRRQAEYQATLTRMITAPLLGIIPYLSDIEENPVTTRRDLGHYLDLTVLRAAEREAVNM
>Mature_239_residues
TKRWFITGTDTDVGKTVASCALLQAATAQGYRTAGYKPVASGSQMTADGLRNSDALALQANSSQRLGYSQVNPFTFLEAT
SPHIASESEGRAIPLTALSQGLRQLEPSADWILIEGAGGWFTPLSPQATFADWVQQEQLPVIMVVGVKLGCINHALLTAQ
AIQHAGLTLAGWVANEVTPAGRRQAEYQATLTRMITAPLLGIIPYLSDIEENPVTTRRDLGHYLDLTVLRAAEREAVNM

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI1786995, Length=230, Percent_Identity=71.304347826087, Blast_Score=332, Evalue=2e-92,
Organism=Escherichia coli, GI87081944, Length=232, Percent_Identity=49.5689655172414, Blast_Score=211, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD_YERPE (Q8ZGW9)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AH0141
- RefSeq:   NP_670328.1
- RefSeq:   NP_992380.1
- RefSeq:   YP_002346193.1
- ProteinModelPortal:   Q8ZGW9
- SMR:   Q8ZGW9
- IntAct:   Q8ZGW9
- GeneID:   1147975
- GeneID:   1174003
- GeneID:   2764809
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO1154
- KEGG:   ypk:y3028
- KEGG:   ypm:YP_1006
- HOGENOM:   HBG650065
- OMA:   PQMERIE
- ProtClustDB:   PRK00090
- BioCyc:   YPES187410:Y3028-MONOMER
- BioCyc:   YPES214092:YPO1154-MONOMER
- BRENDA:   6.3.3.3
- HAMAP:   MF_00336
- InterPro:   IPR004472
- InterPro:   IPR002586
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: PF01656 CbiA

EC number: =6.3.3.3

Molecular weight: Translated: 25821; Mature: 25689

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRWFITGTDTDVGKTVASCALLQAATAQGYRTAGYKPVASGSQMTADGLRNSDALALQ
CCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
ANSSQRLGYSQVNPFTFLEATSPHIASESEGRAIPLTALSQGLRQLEPSADWILIEGAGG
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCEEEEECCCC
WFTPLSPQATFADWVQQEQLPVIMVVGVKLGCINHALLTAQAIQHAGLTLAGWVANEVTP
CCCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEHHHHCCCC
AGRRQAEYQATLTRMITAPLLGIIPYLSDIEENPVTTRRDLGHYLDLTVLRAAEREAVNM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKRWFITGTDTDVGKTVASCALLQAATAQGYRTAGYKPVASGSQMTADGLRNSDALALQ
CCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
ANSSQRLGYSQVNPFTFLEATSPHIASESEGRAIPLTALSQGLRQLEPSADWILIEGAGG
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCEEEEECCCC
WFTPLSPQATFADWVQQEQLPVIMVVGVKLGCINHALLTAQAIQHAGLTLAGWVANEVTP
CCCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEHHHHCCCC
AGRRQAEYQATLTRMITAPLLGIIPYLSDIEENPVTTRRDLGHYLDLTVLRAAEREAVNM
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430