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The map label for this gene is lpxB

Identifier: 218928225

GI number: 218928225

Start: 1198939

End: 1200123

Strand: Direct

Name: lpxB

Synonym: YPO1057

Alternate gene names: 218928225

Gene position: 1198939-1200123 (Clockwise)

Preceding gene: 218928224

Following gene: 218928226

Centisome position: 25.76

GC content: 49.03

Gene sequence:

>1185_bases
ATGCAAAATAGCCCATTGACTGCTGATTGTTCATTAAACGCTGGACGTCCCCTAACGATTGGTTTAGTTGCCGGAGAAAC
TTCCGGCGATATCTTAGGCGCTGGATTAATTCGGGCGCTGAAAGTTCAGGTACCCAATGCCCGATTTGTCGGTGTCGCTG
GGCCTCTGATGCAAGCCGAAGGGTGTGAAGCCTGGTATGAGATGGAAGAGTTAGCCGTCATGGGCGTTGTTGAAGTATTA
GAGCGCCTGCCACGGTTATTGAAAATCAGGAAGGATCTGACTCAGCGTTTTAGTGAGCTTTCTCCCGATGTCTTTGTTGG
CATTGACGCCCCCGATTTCAACATCACCCTTGAAGGGCGCTTAAAACAACGGGGTATTCGGACTATTCATTATGTCAGCC
CATCAGTCTGGGCCTGGCGACAAAAACGTGTTTTCAAAATTGGAAAAGCGACCGACATGGTGTTGGCTTTTCTCCCTTTT
GAAAAAGCGTTTTATGATCGTTTCAATGTCCCTTGCCGTTTTATAGGCCATACCATGGCCGATGCCATGCCACTAGTACC
CGATCAGCAGGCAGCGAGAGCAGAGCTTGGCATTGCGCCTAATGCCACTTGTCTGGCGCTGTTACCGGGTAGCCGCCACT
CTGAAGTTGAAATGCTCAGCGCTGATTTTTTACGTACAGCGGTAATTCTGCGTGATAAATTGCCCAATTTGGAAGTAGTG
GTTCCCTTGGTCAACAGCAAGCGACGTGAACAATTCGAGCGAATTAAAGCGGAAATTGCGCCTGATTTATCCGTACATTT
ACTGGATGGTAAAGCGCGTGTCGCGATGATCGCCAGTGATGCAGCATTGTTAGCCTCAGGCACGGCTGCACTCGAATGTA
TGCTGGCCAAATGCCCGATGGTTGTGGGGTATCGTATGAAGCCATTTACTTTCTGGTTGGCAGAGCGATTAGTCAAAACA
CCTTATGTTTCATTACCGAATCTGTTAGCAGGGGAAGAGTTGGTGACAGAATTGTTGCAACAAGAGTGCCAGCCGCAAAA
ACTCGCTGGTGCACTTTTGCCGTTGCTACAAGGTGGTAGCGAAATAGCAGCCTTAAAAGAACGTTTTTTGGTTCTGCATC
AGAGCATCCGCTGTGGTGCGGATGAACAGGCAGCACAAGCTGTATTGGAGCTGGCAGATCGATGA

Upstream 100 bases:

>100_bases
AGTGAAACCAGAAATTGCTGAATTAGCGGAGCAATATCCAGTCGTTAAAGCGTTCAATGATTTCTTTGCTCGTTCGACCC
GCGGTATTATTCGCTGACTT

Downstream 100 bases:

>100_bases
GCGAGACTTTTATTTACCCACAGGCTAATTTAATCGCAGGCGTGGATGAAGTGGGTCGTGGCCCTTTGGTGGGTGCCGTA
GTCACTGCAGCGGTGATCCT

Product: lipid-A-disaccharide synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MQNSPLTADCSLNAGRPLTIGLVAGETSGDILGAGLIRALKVQVPNARFVGVAGPLMQAEGCEAWYEMEELAVMGVVEVL
ERLPRLLKIRKDLTQRFSELSPDVFVGIDAPDFNITLEGRLKQRGIRTIHYVSPSVWAWRQKRVFKIGKATDMVLAFLPF
EKAFYDRFNVPCRFIGHTMADAMPLVPDQQAARAELGIAPNATCLALLPGSRHSEVEMLSADFLRTAVILRDKLPNLEVV
VPLVNSKRREQFERIKAEIAPDLSVHLLDGKARVAMIASDAALLASGTAALECMLAKCPMVVGYRMKPFTFWLAERLVKT
PYVSLPNLLAGEELVTELLQQECQPQKLAGALLPLLQGGSEIAALKERFLVLHQSIRCGADEQAAQAVLELADR

Sequences:

>Translated_394_residues
MQNSPLTADCSLNAGRPLTIGLVAGETSGDILGAGLIRALKVQVPNARFVGVAGPLMQAEGCEAWYEMEELAVMGVVEVL
ERLPRLLKIRKDLTQRFSELSPDVFVGIDAPDFNITLEGRLKQRGIRTIHYVSPSVWAWRQKRVFKIGKATDMVLAFLPF
EKAFYDRFNVPCRFIGHTMADAMPLVPDQQAARAELGIAPNATCLALLPGSRHSEVEMLSADFLRTAVILRDKLPNLEVV
VPLVNSKRREQFERIKAEIAPDLSVHLLDGKARVAMIASDAALLASGTAALECMLAKCPMVVGYRMKPFTFWLAERLVKT
PYVSLPNLLAGEELVTELLQQECQPQKLAGALLPLLQGGSEIAALKERFLVLHQSIRCGADEQAAQAVLELADR
>Mature_394_residues
MQNSPLTADCSLNAGRPLTIGLVAGETSGDILGAGLIRALKVQVPNARFVGVAGPLMQAEGCEAWYEMEELAVMGVVEVL
ERLPRLLKIRKDLTQRFSELSPDVFVGIDAPDFNITLEGRLKQRGIRTIHYVSPSVWAWRQKRVFKIGKATDMVLAFLPF
EKAFYDRFNVPCRFIGHTMADAMPLVPDQQAARAELGIAPNATCLALLPGSRHSEVEMLSADFLRTAVILRDKLPNLEVV
VPLVNSKRREQFERIKAEIAPDLSVHLLDGKARVAMIASDAALLASGTAALECMLAKCPMVVGYRMKPFTFWLAERLVKT
PYVSLPNLLAGEELVTELLQQECQPQKLAGALLPLLQGGSEIAALKERFLVLHQSIRCGADEQAAQAVLELADR

Specific function: Condensation of UDP-2,3-diacylglucosamine and 2,3- diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell

COG id: COG0763

COG function: function code M; Lipid A disaccharide synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lpxB family

Homologues:

Organism=Escherichia coli, GI1786379, Length=377, Percent_Identity=82.4933687002653, Blast_Score=617, Evalue=1e-178,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LPXB_YERPE (Q8ZH55)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AH0129
- RefSeq:   NP_670421.1
- RefSeq:   NP_994100.1
- RefSeq:   YP_002346100.1
- ProteinModelPortal:   Q8ZH55
- GeneID:   1148069
- GeneID:   1173897
- GeneID:   2765716
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO1057
- KEGG:   ypk:y3122
- KEGG:   ypm:YP_2793
- HOGENOM:   HBG584837
- OMA:   DFTHRVA
- ProtClustDB:   PRK00025
- BioCyc:   YPES187410:Y3122-MONOMER
- BioCyc:   YPES214092:YPO1057-MONOMER
- BRENDA:   2.4.1.182
- HAMAP:   MF_00392
- InterPro:   IPR003835
- TIGRFAMs:   TIGR00215

Pfam domain/function: PF02684 LpxB

EC number: =2.4.1.182

Molecular weight: Translated: 43261; Mature: 43261

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNSPLTADCSLNAGRPLTIGLVAGETSGDILGAGLIRALKVQVPNARFVGVAGPLMQAE
CCCCCCCEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHEECCCCEEEECCCCCCCCC
GCEAWYEMEELAVMGVVEVLERLPRLLKIRKDLTQRFSELSPDVFVGIDAPDFNITLEGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEECC
LKQRGIRTIHYVSPSVWAWRQKRVFKIGKATDMVLAFLPFEKAFYDRFNVPCRFIGHTMA
HHHCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHH
DAMPLVPDQQAARAELGIAPNATCLALLPGSRHSEVEMLSADFLRTAVILRDKLPNLEVV
HCCCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEE
VPLVNSKRREQFERIKAEIAPDLSVHLLDGKARVAMIASDAALLASGTAALECMLAKCPM
EECCCCHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCHHHHHCCHHHHHHHHHHCCH
VVGYRMKPFTFWLAERLVKTPYVSLPNLLAGEELVTELLQQECQPQKLAGALLPLLQGGS
HHCCCCCHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCH
EIAALKERFLVLHQSIRCGADEQAAQAVLELADR
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MQNSPLTADCSLNAGRPLTIGLVAGETSGDILGAGLIRALKVQVPNARFVGVAGPLMQAE
CCCCCCCEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHEECCCCEEEECCCCCCCCC
GCEAWYEMEELAVMGVVEVLERLPRLLKIRKDLTQRFSELSPDVFVGIDAPDFNITLEGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEECC
LKQRGIRTIHYVSPSVWAWRQKRVFKIGKATDMVLAFLPFEKAFYDRFNVPCRFIGHTMA
HHHCCCCEEEECCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHH
DAMPLVPDQQAARAELGIAPNATCLALLPGSRHSEVEMLSADFLRTAVILRDKLPNLEVV
HCCCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEE
VPLVNSKRREQFERIKAEIAPDLSVHLLDGKARVAMIASDAALLASGTAALECMLAKCPM
EECCCCHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCHHHHHCCHHHHHHHHHHCCH
VVGYRMKPFTFWLAERLVKTPYVSLPNLLAGEELVTELLQQECQPQKLAGALLPLLQGGS
HHCCCCCHHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCH
EIAALKERFLVLHQSIRCGADEQAAQAVLELADR
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430