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The map label for this gene is gutA [H]

Identifier: 218928165

GI number: 218928165

Start: 1105824

End: 1107257

Strand: Reverse

Name: gutA [H]

Synonym: YPO0995

Alternate gene names: 218928165

Gene position: 1107257-1105824 (Counterclockwise)

Preceding gene: 218928168

Following gene: 218928160

Centisome position: 23.79

GC content: 44.49

Gene sequence:

>1434_bases
GTGACTGGGATGAGCCTGGACAGCCAGCACCTCTGTAGCTTGAAGGACGAAGGGGATAAAAGGCTAAAAATAAATATGAA
CAATTCAGGCATGAAAATCCCAATGAAAGAGCTGGCGTCTTATTTTGGGTATGGGATGGGGCAGTGTTTCAGTTTTGGGT
TGGTTGGGACTTTTATTCTCTTTTTCTATACTGACATTATGGGGATCTCGCCGGTAGCAGCCAGTATGATCTTTTTAATT
GCCAGGGTATGGGATGCTATACATGACCCATTAATTGCCGGTGTAATGGATACTATTAATATGCGGCGCGGGAAATTCCG
CCCATATTTGCTGTTTACTCCCTTCTTGATTTTCCTTGTTACGGTCGCTGCGTTTTATAATATCGAAGCCAGCTTGATGA
CTAAAACGATCTACGCAGGTGTGACTTATATCCTGTGGGGGACGCTCTACGCTCTCTCTGATATCCCGTTTTGGTCAATG
AGCACCGTGATGACGGATGAACCGCAAGAGCGAGCTAAAACAGCGACCTGCGCGATGTTGGGCGTGAATGCGGGTATCGG
CGCTACGATGATTTTATTCCCTTATATCAGCGGGTTATTTGCTGAGAACAGTGCTGATCGTGGTTATTTTGCGGGTGTTG
TTATCCTCATGGTTTTGGGGGTGATACTCATGTTGAATGGGTTCTTTAATACAAAAGAACGCGTCAACGTCACGGTGACG
GAAAAGGTCACGCTAAAGCAGACATTTATTGTGGTCTGGCAAAATAAGCCGCTATTTTTTATTCTTAGCGCCTTTTTCAT
GAATGTGTTTTCTAACATCGTGAATACTTTTTATATTTTCTTTTTCACTTATAACATGGGGGATGCTGAGCTGGTTTCTG
TTATCGGCTTAATTACATTCACCTGTGCTTTAGCTTGTCTGGGAACGCCATTCTTAACCCGCCATTTTAAGAAACGAGAT
TTGTTCATTACATTATGTGTGTTGGAGATCATCGCTCGCGTTGGTTTCTGGTTCACCGGTTATAATAATGTCGTGTCAGT
CATGGTATGGCTAACCGTGATCACTGCCATCTTCATGATGACGAATCCACTTATTTCCGCGATGATTGCCGATACTGTGG
AATATTCCTACTATCACACCGGTAAGCGCTGCGCGGCCATCACGTTCTCCGGACAGACTTTTGTCGGTAAATTGTCGGTC
GCTGTTGCTGGCGGTGTCTCTGGCCTGATCCTGTCAATATTGGGATATATGCCTAATGTGGCCCAATCGACATGGACATT
GAATGGCCTATTTTTCTGTATTTCTCTGTTGCCCGCCGTGGGTGCCGTGGTGCGTATCCTCATTATGCGTAAATATAAAT
TTACCGAAGATGAACATGCAATTCTTCGTGAAGAACTGAAACAAGGGAGATTCCACTCTTCAGTAGGCAAATAA

Upstream 100 bases:

>100_bases
CATTAAAAATTTATCTGTACCTTACATAATATATACCCAAGTGACTTCGAGATGCAGGTAGGCGGCAACCGAGTAAATTC
CCAGGAGCTTAGAGAACTAA

Downstream 100 bases:

>100_bases
AGTGATTCTCAGTAGGGCGTGAATGGATTAAAGCCGCCAGAATAAACCGCTATGGTGTGAGAGGGTCTTAATTAACGCGT
TTGTAGTAATAAACGATAGG

Product: Sodium:galactoside symporter family protein

Products: Li (I) [Cytoplasm]; melibiose [Cytoplasm]; Na (I) [Cytoplasm]; melibiose [Cytoplasm]; Proton [Cytoplasm]; melibiose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MTGMSLDSQHLCSLKDEGDKRLKINMNNSGMKIPMKELASYFGYGMGQCFSFGLVGTFILFFYTDIMGISPVAASMIFLI
ARVWDAIHDPLIAGVMDTINMRRGKFRPYLLFTPFLIFLVTVAAFYNIEASLMTKTIYAGVTYILWGTLYALSDIPFWSM
STVMTDEPQERAKTATCAMLGVNAGIGATMILFPYISGLFAENSADRGYFAGVVILMVLGVILMLNGFFNTKERVNVTVT
EKVTLKQTFIVVWQNKPLFFILSAFFMNVFSNIVNTFYIFFFTYNMGDAELVSVIGLITFTCALACLGTPFLTRHFKKRD
LFITLCVLEIIARVGFWFTGYNNVVSVMVWLTVITAIFMMTNPLISAMIADTVEYSYYHTGKRCAAITFSGQTFVGKLSV
AVAGGVSGLILSILGYMPNVAQSTWTLNGLFFCISLLPAVGAVVRILIMRKYKFTEDEHAILREELKQGRFHSSVGK

Sequences:

>Translated_477_residues
MTGMSLDSQHLCSLKDEGDKRLKINMNNSGMKIPMKELASYFGYGMGQCFSFGLVGTFILFFYTDIMGISPVAASMIFLI
ARVWDAIHDPLIAGVMDTINMRRGKFRPYLLFTPFLIFLVTVAAFYNIEASLMTKTIYAGVTYILWGTLYALSDIPFWSM
STVMTDEPQERAKTATCAMLGVNAGIGATMILFPYISGLFAENSADRGYFAGVVILMVLGVILMLNGFFNTKERVNVTVT
EKVTLKQTFIVVWQNKPLFFILSAFFMNVFSNIVNTFYIFFFTYNMGDAELVSVIGLITFTCALACLGTPFLTRHFKKRD
LFITLCVLEIIARVGFWFTGYNNVVSVMVWLTVITAIFMMTNPLISAMIADTVEYSYYHTGKRCAAITFSGQTFVGKLSV
AVAGGVSGLILSILGYMPNVAQSTWTLNGLFFCISLLPAVGAVVRILIMRKYKFTEDEHAILREELKQGRFHSSVGK
>Mature_476_residues
TGMSLDSQHLCSLKDEGDKRLKINMNNSGMKIPMKELASYFGYGMGQCFSFGLVGTFILFFYTDIMGISPVAASMIFLIA
RVWDAIHDPLIAGVMDTINMRRGKFRPYLLFTPFLIFLVTVAAFYNIEASLMTKTIYAGVTYILWGTLYALSDIPFWSMS
TVMTDEPQERAKTATCAMLGVNAGIGATMILFPYISGLFAENSADRGYFAGVVILMVLGVILMLNGFFNTKERVNVTVTE
KVTLKQTFIVVWQNKPLFFILSAFFMNVFSNIVNTFYIFFFTYNMGDAELVSVIGLITFTCALACLGTPFLTRHFKKRDL
FITLCVLEIIARVGFWFTGYNNVVSVMVWLTVITAIFMMTNPLISAMIADTVEYSYYHTGKRCAAITFSGQTFVGKLSVA
VAGGVSGLILSILGYMPNVAQSTWTLNGLFFCISLLPAVGAVVRILIMRKYKFTEDEHAILREELKQGRFHSSVGK

Specific function: Probably involved in glucitol uptake [H]

COG id: COG2211

COG function: function code G; Na+/melibiose symporter and related transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:galactoside symporter (TC 2.A.2) family [H]

Homologues:

Organism=Escherichia coli, GI1790561, Length=449, Percent_Identity=30.7349665924276, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI48994989, Length=458, Percent_Identity=28.6026200873362, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI87082306, Length=451, Percent_Identity=28.159645232816, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI145693206, Length=453, Percent_Identity=28.9183222958057, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1787902, Length=444, Percent_Identity=29.2792792792793, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1786466, Length=434, Percent_Identity=27.6497695852535, Blast_Score=144, Evalue=9e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001927
- InterPro:   IPR018043 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 53204; Mature: 53073

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGMSLDSQHLCSLKDEGDKRLKINMNNSGMKIPMKELASYFGYGMGQCFSFGLVGTFIL
CCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
FFYTDIMGISPVAASMIFLIARVWDAIHDPLIAGVMDTINMRRGKFRPYLLFTPFLIFLV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
TVAAFYNIEASLMTKTIYAGVTYILWGTLYALSDIPFWSMSTVMTDEPQERAKTATCAML
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHE
GVNAGIGATMILFPYISGLFAENSADRGYFAGVVILMVLGVILMLNGFFNTKERVNVTVT
ECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
EKVTLKQTFIVVWQNKPLFFILSAFFMNVFSNIVNTFYIFFFTYNMGDAELVSVIGLITF
CEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEHHEEECCCHHHHHHHHHHHHH
TCALACLGTPFLTRHFKKRDLFITLCVLEIIARVGFWFTGYNNVVSVMVWLTVITAIFMM
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHH
TNPLISAMIADTVEYSYYHTGKRCAAITFSGQTFVGKLSVAVAGGVSGLILSILGYMPNV
HHHHHHHHHHHHHHHHHEECCCEEEEEEECCCEEEHHHHHHHHCCHHHHHHHHHHHCCCH
AQSTWTLNGLFFCISLLPAVGAVVRILIMRKYKFTEDEHAILREELKQGRFHSSVGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure 
TGMSLDSQHLCSLKDEGDKRLKINMNNSGMKIPMKELASYFGYGMGQCFSFGLVGTFIL
CCCCCCHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHH
FFYTDIMGISPVAASMIFLIARVWDAIHDPLIAGVMDTINMRRGKFRPYLLFTPFLIFLV
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
TVAAFYNIEASLMTKTIYAGVTYILWGTLYALSDIPFWSMSTVMTDEPQERAKTATCAML
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHE
GVNAGIGATMILFPYISGLFAENSADRGYFAGVVILMVLGVILMLNGFFNTKERVNVTVT
ECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
EKVTLKQTFIVVWQNKPLFFILSAFFMNVFSNIVNTFYIFFFTYNMGDAELVSVIGLITF
CEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEHHEEECCCHHHHHHHHHHHHH
TCALACLGTPFLTRHFKKRDLFITLCVLEIIARVGFWFTGYNNVVSVMVWLTVITAIFMM
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHH
TNPLISAMIADTVEYSYYHTGKRCAAITFSGQTFVGKLSVAVAGGVSGLILSILGYMPNV
HHHHHHHHHHHHHHHHHEECCCEEEEEEECCCEEEHHHHHHHHCCHHHHHHHHHHHCCCH
AQSTWTLNGLFFCISLLPAVGAVVRILIMRKYKFTEDEHAILREELKQGRFHSSVGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Li (I) [Periplasm]; melibiose [Periplasm]; Na (I) [Periplasm]; melibiose [Periplasm]; Proton [Periplasm]; melibiose [Periplasm] [C]

Specific reaction: Li (I) [Periplasm] + melibiose [Periplasm] = Li (I) [Cytoplasm] + melibiose [Cytoplasm] Na (I) [Periplasm] + melibiose [Periplasm] = Na (I) [Cytoplasm] + melibiose [Cytoplasm] Proton [Periplasm] + melibiose [Periplasm] = Proton [Cytoplasm] + melibiose [Cy

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9455482; 9384377 [H]