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The map label for this gene is gcsH [H]

Identifier: 218928079

GI number: 218928079

Start: 994379

End: 994765

Strand: Reverse

Name: gcsH [H]

Synonym: YPO0906

Alternate gene names: 218928079

Gene position: 994765-994379 (Counterclockwise)

Preceding gene: 218928080

Following gene: 218928078

Centisome position: 21.38

GC content: 49.35

Gene sequence:

>387_bases
ATGAGTAATGTACCAACAGAATTGAAATATGCCTTATCTCATGAATGGGTTCGTGCTGACGGCGATGGTGTGTATAGCGT
CGGGATCACTGAACATGCGCAAGAGCTGCTGGGTGATATGGTATTCGTCGATCTGCCAGAGGTGGGCAGCGATGTTTCGG
CCGGTAGCGATTGTGCGGTTGCCGAATCGGTGAAAGCCGCTTCCGATATTTACGCGCCTATCAGCGGTGAAATCGTTGCA
GTTAATACTGAGTTGGAAAACTCGCCAGAATTGGTTAACAGCGCGCCTTATACCGATGGTTGGTTGTTCAGTATTAAAGC
CGCTGATGAGTCCGAACTGGACAATCTGTTGGATGCTGATGCTTACCTCGCGGCGATTGAAGAGTAA

Upstream 100 bases:

>100_bases
TTGTGCGAGCGGGCAAAGCCATCGTATTGTAAGTTTGTTTTCCATTATTCTGAATTATAAGAGCCTTCTCTTTTGGCTCT
ATCTCTTTTAAGGAATAGCA

Downstream 100 bases:

>100_bases
CTCAAGTGAGGGCCATTTTATTGGCCCTGACGCCGAGTAGGTTAAATGGCGCTACAGCCCGGTGCAAGCGAACGTCCCCG
ATGAGCCTATCCAATATAGG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 127

Protein sequence:

>128_residues
MSNVPTELKYALSHEWVRADGDGVYSVGITEHAQELLGDMVFVDLPEVGSDVSAGSDCAVAESVKAASDIYAPISGEIVA
VNTELENSPELVNSAPYTDGWLFSIKAADESELDNLLDADAYLAAIEE

Sequences:

>Translated_128_residues
MSNVPTELKYALSHEWVRADGDGVYSVGITEHAQELLGDMVFVDLPEVGSDVSAGSDCAVAESVKAASDIYAPISGEIVA
VNTELENSPELVNSAPYTDGWLFSIKAADESELDNLLDADAYLAAIEE
>Mature_127_residues
SNVPTELKYALSHEWVRADGDGVYSVGITEHAQELLGDMVFVDLPEVGSDVSAGSDCAVAESVKAASDIYAPISGEIVAV
NTELENSPELVNSAPYTDGWLFSIKAADESELDNLLDADAYLAAIEE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=123, Percent_Identity=43.9024390243902, Blast_Score=108, Evalue=1e-24,
Organism=Homo sapiens, GI89057342, Length=123, Percent_Identity=43.0894308943089, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1789271, Length=128, Percent_Identity=75.78125, Blast_Score=199, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI17507493, Length=113, Percent_Identity=46.9026548672566, Blast_Score=113, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17551294, Length=113, Percent_Identity=44.2477876106195, Blast_Score=109, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6319272, Length=124, Percent_Identity=38.7096774193548, Blast_Score=98, Evalue=3e-22,
Organism=Drosophila melanogaster, GI17865652, Length=118, Percent_Identity=44.0677966101695, Blast_Score=111, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13581; Mature: 13450

Theoretical pI: Translated: 3.53; Mature: 3.53

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVPTELKYALSHEWVRADGDGVYSVGITEHAQELLGDMVFVDLPEVGSDVSAGSDCAV
CCCCCCHHHHHHCCCEEEECCCCEEEECHHHHHHHHHHCEEEEECCCCCCCCCCCCCCHH
AESVKAASDIYAPISGEIVAVNTELENSPELVNSAPYTDGWLFSIKAADESELDNLLDAD
HHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHCCCCCCCCEEEEEECCCHHHHHHHHCCH
AYLAAIEE
HHEEEECC
>Mature Secondary Structure 
SNVPTELKYALSHEWVRADGDGVYSVGITEHAQELLGDMVFVDLPEVGSDVSAGSDCAV
CCCCCHHHHHHCCCEEEECCCCEEEECHHHHHHHHHHCEEEEECCCCCCCCCCCCCCHH
AESVKAASDIYAPISGEIVAVNTELENSPELVNSAPYTDGWLFSIKAADESELDNLLDAD
HHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHCCCCCCCCEEEEEECCCHHHHHHHHCCH
AYLAAIEE
HHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA