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The map label for this gene is galR [H]

Identifier: 218927975

GI number: 218927975

Start: 874348

End: 875370

Strand: Direct

Name: galR [H]

Synonym: YPO0795

Alternate gene names: 218927975

Gene position: 874348-875370 (Clockwise)

Preceding gene: 218927970

Following gene: 218927977

Centisome position: 18.79

GC content: 52.2

Gene sequence:

>1023_bases
ATGGCCACTATAAAGGATGTTGCCAAGCTGGCGGGTGTTTCCGTCGCAACGGTATCTCGTGTTATCAATCATTCTCCCAA
AGCCAGTGAAGCATCACGCGTGGCGGTGTGCAAGGCGATGGAACAACTGCAATACCACCCGAATGCCAACGCCCGAGCAC
TGGCGCAACAATCGACAGAAACGGTCGGTATGATTGTGTCTGATGTCTCGGACCCTTTCTTCGGTGCGATGGTGAAAGCC
GTCGAACAAGTCGCGTATGCCACCGGTAATTTCCTGTTAATTGGCAACGGTTACCATGATGCCGAAAAAGAACGTCAGGC
CATCGAACAACTCATTCGCCACCGCTGCGCTGCGCTGGTGGTACATGCCAAAAAATTACCCGATGACGAACTGACGTCAT
TAATGGAACAAATTCCTGGCATGGTGTTAATTAACCGCACCTTACCGGGCTTCGAACCCCGTTGTATCGCATTAGATGAC
CGCTATGGTGCCTGGCTGGCAACTCGCCATCTCATCCAGCAGGGGCATAAACGGGTCGCGTTCATTTGCTCCAATCATCA
GATTTCCGATGCGCTTGACCGGATGCAAGGCTATTTGGATGCGTTGAAAGAATTTGATATCCCGGTTGATGAGCGTTTAA
TTACCTACGGCACCCCCGACGAACTCGGCGGTGAGCAGGCAATGACCGATCTACTTGGCCGTGGTAAACACTTCACCGCG
GTAAGCTGTTATAACGACTCAATGGCGGCCGGGGCGTTATCGGTTCTCAGTGATAACAGTATTGATGTGCCACAGGAGAT
TTCACTCATCGGTTTTGATGATGTATTAATCTCCCGTTACCTGCGCCCACGCCTGACGACAATCCGTTACCCAGTCGTTG
CCATGTCTACCCAGGCCGCTGAGTTGGCACTCGCATTAGCCAACAACACACCGTTACCCGAAATTACCAATATGTTCAGC
CCAACACTGGTTCGCCGCCACTCTGTGGCCAGCCCGCCAAGCCTACGGGATGACACTGATTAA

Upstream 100 bases:

>100_bases
GTTTTTCTTACTAAGTTGCGACCATCCCCCCTTGCGGGTAATCTTATTTTCCGCAAAACTGAGTGATGTAAGCGGTTACC
CTAGGATCTCTGGAACAAAA

Downstream 100 bases:

>100_bases
ACGCGTTCCAGGTCAATCAATTCTTCAATGGTTTGACGGCGGCGGATCAAGCGCGGTTGGCCCTTTTCAAACAGTACTTC
AGGTAATAACGGGCGGCTGT

Product: DNA-binding transcriptional regulator GalR

Products: NA

Alternate protein names: Galactose operon repressor [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MATIKDVAKLAGVSVATVSRVINHSPKASEASRVAVCKAMEQLQYHPNANARALAQQSTETVGMIVSDVSDPFFGAMVKA
VEQVAYATGNFLLIGNGYHDAEKERQAIEQLIRHRCAALVVHAKKLPDDELTSLMEQIPGMVLINRTLPGFEPRCIALDD
RYGAWLATRHLIQQGHKRVAFICSNHQISDALDRMQGYLDALKEFDIPVDERLITYGTPDELGGEQAMTDLLGRGKHFTA
VSCYNDSMAAGALSVLSDNSIDVPQEISLIGFDDVLISRYLRPRLTTIRYPVVAMSTQAAELALALANNTPLPEITNMFS
PTLVRRHSVASPPSLRDDTD

Sequences:

>Translated_340_residues
MATIKDVAKLAGVSVATVSRVINHSPKASEASRVAVCKAMEQLQYHPNANARALAQQSTETVGMIVSDVSDPFFGAMVKA
VEQVAYATGNFLLIGNGYHDAEKERQAIEQLIRHRCAALVVHAKKLPDDELTSLMEQIPGMVLINRTLPGFEPRCIALDD
RYGAWLATRHLIQQGHKRVAFICSNHQISDALDRMQGYLDALKEFDIPVDERLITYGTPDELGGEQAMTDLLGRGKHFTA
VSCYNDSMAAGALSVLSDNSIDVPQEISLIGFDDVLISRYLRPRLTTIRYPVVAMSTQAAELALALANNTPLPEITNMFS
PTLVRRHSVASPPSLRDDTD
>Mature_339_residues
ATIKDVAKLAGVSVATVSRVINHSPKASEASRVAVCKAMEQLQYHPNANARALAQQSTETVGMIVSDVSDPFFGAMVKAV
EQVAYATGNFLLIGNGYHDAEKERQAIEQLIRHRCAALVVHAKKLPDDELTSLMEQIPGMVLINRTLPGFEPRCIALDDR
YGAWLATRHLIQQGHKRVAFICSNHQISDALDRMQGYLDALKEFDIPVDERLITYGTPDELGGEQAMTDLLGRGKHFTAV
SCYNDSMAAGALSVLSDNSIDVPQEISLIGFDDVLISRYLRPRLTTIRYPVVAMSTQAAELALALANNTPLPEITNMFSP
TLVRRHSVASPPSLRDDTD

Specific function: Repressor of the galactose operon. Binds galactose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789202, Length=332, Percent_Identity=80.7228915662651, Blast_Score=555, Evalue=1e-159,
Organism=Escherichia coli, GI1788474, Length=331, Percent_Identity=56.1933534743202, Blast_Score=371, Evalue=1e-104,
Organism=Escherichia coli, GI1789068, Length=337, Percent_Identity=34.7181008902077, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI1787948, Length=341, Percent_Identity=34.0175953079179, Blast_Score=181, Evalue=4e-47,
Organism=Escherichia coli, GI1790369, Length=303, Percent_Identity=34.3234323432343, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=31.5151515151515, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1787906, Length=317, Percent_Identity=29.0220820189274, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787580, Length=320, Percent_Identity=26.5625, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1786540, Length=336, Percent_Identity=26.7857142857143, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1790715, Length=323, Percent_Identity=24.7678018575851, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI48994940, Length=308, Percent_Identity=22.4025974025974, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1790689, Length=298, Percent_Identity=26.510067114094, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786268, Length=275, Percent_Identity=22.9090909090909, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37213; Mature: 37082

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVAKLAGVSVATVSRVINHSPKASEASRVAVCKAMEQLQYHPNANARALAQQSTE
CCCHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
TVGMIVSDVSDPFFGAMVKAVEQVAYATGNFLLIGNGYHDAEKERQAIEQLIRHRCAALV
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
VHAKKLPDDELTSLMEQIPGMVLINRTLPGFEPRCIALDDRYGAWLATRHLIQQGHKRVA
HHHHCCCHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEE
FICSNHQISDALDRMQGYLDALKEFDIPVDERLITYGTPDELGGEQAMTDLLGRGKHFTA
EEECCCCHHHHHHHHHHHHHHHHHCCCCHHCEEEECCCCHHCCHHHHHHHHHHCCCCEEE
VSCYNDSMAAGALSVLSDNSIDVPQEISLIGFDDVLISRYLRPRLTTIRYPVVAMSTQAA
EEECCCCHHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHEECCHHEECHHHH
ELALALANNTPLPEITNMFSPTLVRRHSVASPPSLRDDTD
HHHHHHCCCCCCHHHHHHHCHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
ATIKDVAKLAGVSVATVSRVINHSPKASEASRVAVCKAMEQLQYHPNANARALAQQSTE
CCHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
TVGMIVSDVSDPFFGAMVKAVEQVAYATGNFLLIGNGYHDAEKERQAIEQLIRHRCAALV
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
VHAKKLPDDELTSLMEQIPGMVLINRTLPGFEPRCIALDDRYGAWLATRHLIQQGHKRVA
HHHHCCCHHHHHHHHHHCCCEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHCCCCEEE
FICSNHQISDALDRMQGYLDALKEFDIPVDERLITYGTPDELGGEQAMTDLLGRGKHFTA
EEECCCCHHHHHHHHHHHHHHHHHCCCCHHCEEEECCCCHHCCHHHHHHHHHHCCCCEEE
VSCYNDSMAAGALSVLSDNSIDVPQEISLIGFDDVLISRYLRPRLTTIRYPVVAMSTQAA
EEECCCCHHHHHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHEECCHHEECHHHH
ELALALANNTPLPEITNMFSPTLVRRHSVASPPSLRDDTD
HHHHHHCCCCCCHHHHHHHCHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6283521; 9278503; 6350601; 8188660; 8982002 [H]