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The map label for this gene is ptsP [H]

Identifier: 218927965

GI number: 218927965

Start: 861441

End: 863687

Strand: Reverse

Name: ptsP [H]

Synonym: YPO0785

Alternate gene names: 218927965

Gene position: 863687-861441 (Counterclockwise)

Preceding gene: 218927966

Following gene: 218927964

Centisome position: 18.56

GC content: 53.4

Gene sequence:

>2247_bases
ATGCTCATGCGTTTGCGAGAAGTAGTTGAGAAAGTGGCGATGGCAACCGGCCTGACGGAGGCGTTAGAGCTGTTGGTCAA
TGAAACCTGTCTGGCGATGGACACGGAAGTCTGTTCGATTTATCTGGCAGACAATGACCGCCGTTGTTACTACCTAATGG
CGACCAGGGGGCTGAAAAAGCCCCGTGGGCGTACTATCACGCTGGCTTTTGACGAGGGGATCGTTGGGCTGGTGGGGCGT
TTGGCCGAGCCAATTAACCTGGCTGATGCTCAAAGCCATCCCAGTTTTAAATATGTCCCGCAAGTCAAAGAGGAACGCTA
TCGGGCGTTTCTCGGGGTGCCCATTATCTACCGCCGCCAGTTGCTTGGTGTGCTGGTGGTTCAACAGCGTGAGCACCGTC
AGTTTGATGAGAGCGAAGAGTCGTTCATGGTCACGCTGGCAACACAGTTGGCGGGCATCCTTTCTCAATCTCAGCTAAAT
GCCATTTTTGGTCAGTATCGCCAAACACGGATCCGTGCATTGGCGGCGGCTCCTGGTGTCGCGGTGGCAGAAGGCTGGCA
AGATATCTCACAGCCTTCGCTTGATCTGGTCTATGAGGCCTCAACGCTTGATAGCACCCTTGAACGTGAACGTCTGACTC
AGGCCTTGGAAGAGGCTGCGGCTGAGTTTCGTCGCTTCAGTAAGCGCTTTGCCGCCAGTTCACAAAAAGAGAGTGCGGCA
ATTTTCGATCTCTACTCCCACTTGCTAAATGATGCTCGCTTAAAGCGTGAGCTATTTGCGCAAATTGATGCAGGCGCGGT
GGCTGAGTGGGCGGTTAAACAGGTGGTTGAGGCGTTTGCTGCCCAGTTTGCCAGTCTGCAAGATACCTATATGCGCGAGC
GTGCTAGTGACCTGCGGGCATTAGGCCAACGCTTGTTGTTCCATCTTGATGACAGTACCTCTGGAGCCAGCCAGTGGCCT
GAGCGCTTTATCCTGGTTGCTGATGAACTTACTGCGACATTGCTCGCCGAGGTGCCACAGGACCGCTTGGCGGGGGTTGT
GGTGCGTGATGGTGCCGCCAACTCTCATGCGGCTATTTTGGTCCGGGCAATGGGCATCCCGACAGTGATGGGGGCGGATA
TCCAGCCTGCATTACTCAGCCAGCGGCTGCTGATTGTTGATGGTTATCGTGGCGAAGTATTAGTTGATCCTGAACCGGTA
CTGGTCAAAGAGTACCAACGGCTGGTCACCGAAGAGATTGAACTCAGTAAGCTGGCTGAAGATGATGTTGAGCAACCTGC
GGCCCTGAAAAGTGGTGAACGGATCCAGGTGATGCTAAACGCGGGCCTTAGCCCAGAGCACGAACAGTTACTGGGGGGCA
GGGTAGATGGTGTAGGCCTGTACCGCACAGAAATACCGTTTATGTTGCAAAGTGGTTTCCCGTCGGAAGAAGAGCAAGTT
GCGCAATACCAAGGCATGTTACAGCTTTATCCACAAAAGCCCGTGACCTTGCGTACCTTGGATATCGGTGCGGATAAGCA
GCTCCCCTACATGCCTATCAGCGAGGAGAACCCTTGTCTGGGCTGGCGCGGCATTCGTATCACCCTGGATCAACCCGAAA
TCTTTTTGATTCAGGTAAGAGCAATGCTCAGAGCTAATGCCGGGACCGGTAATTTAGGTATTTTATTGCCGATGATCACC
AGTATCGAAGAGGTTGATGAAGCGAAACGTTTGATTGACCGTGCGGGTCGCGAAGTAGAGGAAATGCTCGGTTATGTGCT
GCCACAACCTCGGTTGGGGGTGATGATTGAAGTTCCATCCATGATTTTCATGCTGCCTTACTTGACGTCTCGGGTGGATT
TTATCTCGGTTGGTACCAATGATCTGACGCAATATTTACTGGCCGTTGATCGCAACAATACCCGGGTGGCTTCGTTGTAC
GACAGCCTCCACCCAGCGATGTTACAAGTGCTTCGCCAAATTCTGATGCAGGCAACAGAGTCAGGCCTGCAAGTCAGTCT
CTGTGGTGAAATGGCCGGTGATCCGATGGGGGCGTTACTGCTGGTTGGGCTGGGTTACCGTAATCTCAGTATGAACGGCC
GCAGTGTGGCACGCATCAAATACCTGTTACGTAATATAGAATTGGCCGATGCACAAGTGCTGGCGGCACGGGTATTAACC
GCGCAGATGACCACCGATGTGCGTCACCTAACCGCTGCATTTATGGAGCGCCGTGGTTTAGGTGGGCTGATTCGGGGTGG
GAAGTAG

Upstream 100 bases:

>100_bases
AATGAAAGAATTTGCTGCAACCGTAATGCCTGTGCAGGAGGTCGCTCCTCCACGGGTACCGCCCGCTTATCGCCGTAAAA
GAGGTTAAGTTAAGCCGATC

Downstream 100 bases:

>100_bases
AATGCTTATAAATACCAAAAGCCATCGAGGTTGCAGTCATACGGCAAGTGAATGAATATCAATGCGCTGCCTTAGATCAG
TGATTTAAAGGGGCGAGAGC

Product: fused phosphoenolpyruvate-protein phosphotransferase PtsP/GAF domain

Products: NA

Alternate protein names: Enzyme I-Ntr; Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 748; Mature: 748

Protein sequence:

>748_residues
MLMRLREVVEKVAMATGLTEALELLVNETCLAMDTEVCSIYLADNDRRCYYLMATRGLKKPRGRTITLAFDEGIVGLVGR
LAEPINLADAQSHPSFKYVPQVKEERYRAFLGVPIIYRRQLLGVLVVQQREHRQFDESEESFMVTLATQLAGILSQSQLN
AIFGQYRQTRIRALAAAPGVAVAEGWQDISQPSLDLVYEASTLDSTLERERLTQALEEAAAEFRRFSKRFAASSQKESAA
IFDLYSHLLNDARLKRELFAQIDAGAVAEWAVKQVVEAFAAQFASLQDTYMRERASDLRALGQRLLFHLDDSTSGASQWP
ERFILVADELTATLLAEVPQDRLAGVVVRDGAANSHAAILVRAMGIPTVMGADIQPALLSQRLLIVDGYRGEVLVDPEPV
LVKEYQRLVTEEIELSKLAEDDVEQPAALKSGERIQVMLNAGLSPEHEQLLGGRVDGVGLYRTEIPFMLQSGFPSEEEQV
AQYQGMLQLYPQKPVTLRTLDIGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAGTGNLGILLPMIT
SIEEVDEAKRLIDRAGREVEEMLGYVLPQPRLGVMIEVPSMIFMLPYLTSRVDFISVGTNDLTQYLLAVDRNNTRVASLY
DSLHPAMLQVLRQILMQATESGLQVSLCGEMAGDPMGALLLVGLGYRNLSMNGRSVARIKYLLRNIELADAQVLAARVLT
AQMTTDVRHLTAAFMERRGLGGLIRGGK

Sequences:

>Translated_748_residues
MLMRLREVVEKVAMATGLTEALELLVNETCLAMDTEVCSIYLADNDRRCYYLMATRGLKKPRGRTITLAFDEGIVGLVGR
LAEPINLADAQSHPSFKYVPQVKEERYRAFLGVPIIYRRQLLGVLVVQQREHRQFDESEESFMVTLATQLAGILSQSQLN
AIFGQYRQTRIRALAAAPGVAVAEGWQDISQPSLDLVYEASTLDSTLERERLTQALEEAAAEFRRFSKRFAASSQKESAA
IFDLYSHLLNDARLKRELFAQIDAGAVAEWAVKQVVEAFAAQFASLQDTYMRERASDLRALGQRLLFHLDDSTSGASQWP
ERFILVADELTATLLAEVPQDRLAGVVVRDGAANSHAAILVRAMGIPTVMGADIQPALLSQRLLIVDGYRGEVLVDPEPV
LVKEYQRLVTEEIELSKLAEDDVEQPAALKSGERIQVMLNAGLSPEHEQLLGGRVDGVGLYRTEIPFMLQSGFPSEEEQV
AQYQGMLQLYPQKPVTLRTLDIGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAGTGNLGILLPMIT
SIEEVDEAKRLIDRAGREVEEMLGYVLPQPRLGVMIEVPSMIFMLPYLTSRVDFISVGTNDLTQYLLAVDRNNTRVASLY
DSLHPAMLQVLRQILMQATESGLQVSLCGEMAGDPMGALLLVGLGYRNLSMNGRSVARIKYLLRNIELADAQVLAARVLT
AQMTTDVRHLTAAFMERRGLGGLIRGGK
>Mature_748_residues
MLMRLREVVEKVAMATGLTEALELLVNETCLAMDTEVCSIYLADNDRRCYYLMATRGLKKPRGRTITLAFDEGIVGLVGR
LAEPINLADAQSHPSFKYVPQVKEERYRAFLGVPIIYRRQLLGVLVVQQREHRQFDESEESFMVTLATQLAGILSQSQLN
AIFGQYRQTRIRALAAAPGVAVAEGWQDISQPSLDLVYEASTLDSTLERERLTQALEEAAAEFRRFSKRFAASSQKESAA
IFDLYSHLLNDARLKRELFAQIDAGAVAEWAVKQVVEAFAAQFASLQDTYMRERASDLRALGQRLLFHLDDSTSGASQWP
ERFILVADELTATLLAEVPQDRLAGVVVRDGAANSHAAILVRAMGIPTVMGADIQPALLSQRLLIVDGYRGEVLVDPEPV
LVKEYQRLVTEEIELSKLAEDDVEQPAALKSGERIQVMLNAGLSPEHEQLLGGRVDGVGLYRTEIPFMLQSGFPSEEEQV
AQYQGMLQLYPQKPVTLRTLDIGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAGTGNLGILLPMIT
SIEEVDEAKRLIDRAGREVEEMLGYVLPQPRLGVMIEVPSMIFMLPYLTSRVDFISVGTNDLTQYLLAVDRNNTRVASLY
DSLHPAMLQVLRQILMQATESGLQVSLCGEMAGDPMGALLLVGLGYRNLSMNGRSVARIKYLLRNIELADAQVLAARVLT
AQMTTDVRHLTAAFMERRGLGGLIRGGK

Specific function: Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP)

COG id: COG3605

COG function: function code T; Signal transduction protein containing GAF and PtsI domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GAF domain [H]

Homologues:

Organism=Escherichia coli, GI1789193, Length=747, Percent_Identity=79.2503346720214, Blast_Score=1241, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=541, Percent_Identity=35.1201478743068, Blast_Score=305, Evalue=6e-84,
Organism=Escherichia coli, GI48994992, Length=509, Percent_Identity=32.2200392927308, Blast_Score=256, Evalue=4e-69,
Organism=Escherichia coli, GI1788726, Length=521, Percent_Identity=32.6295585412668, Blast_Score=233, Evalue=3e-62,
Organism=Escherichia coli, GI1787994, Length=408, Percent_Identity=25.7352941176471, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI226510935, Length=207, Percent_Identity=28.0193236714976, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003018
- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF01590 GAF; PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 83051; Mature: 83051

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMRLREVVEKVAMATGLTEALELLVNETCLAMDTEVCSIYLADNDRRCYYLMATRGLKK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEECCCEEEEEEECCCCEEEEEEEECCCCC
PRGRTITLAFDEGIVGLVGRLAEPINLADAQSHPSFKYVPQVKEERYRAFLGVPIIYRRQ
CCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHH
LLGVLVVQQREHRQFDESEESFMVTLATQLAGILSQSQLNAIFGQYRQTRIRALAAAPGV
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AVAEGWQDISQPSLDLVYEASTLDSTLERERLTQALEEAAAEFRRFSKRFAASSQKESAA
HHHHCHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
IFDLYSHLLNDARLKRELFAQIDAGAVAEWAVKQVVEAFAAQFASLQDTYMRERASDLRA
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGQRLLFHLDDSTSGASQWPERFILVADELTATLLAEVPQDRLAGVVVRDGAANSHAAIL
HHHHHEEEECCCCCCCCCCCHHEEEEHHHHHHHHHHHCCHHHHCEEEEECCCCCCCCEEE
VRAMGIPTVMGADIQPALLSQRLLIVDGYRGEVLVDPEPVLVKEYQRLVTEEIELSKLAE
EEECCCCCCCCCCCCHHHHCCCEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHH
DDVEQPAALKSGERIQVMLNAGLSPEHEQLLGGRVDGVGLYRTEIPFMLQSGFPSEEEQV
HHHCCCHHHCCCCEEEEEEECCCCCCHHHHHCCCCCCCEEEECCCCHHHHCCCCCCHHHH
AQYQGMLQLYPQKPVTLRTLDIGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVR
HHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEHHHHH
AMLRANAGTGNLGILLPMITSIEEVDEAKRLIDRAGREVEEMLGYVLPQPRLGVMIEVPS
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEEECHH
MIFMLPYLTSRVDFISVGTNDLTQYLLAVDRNNTRVASLYDSLHPAMLQVLRQILMQATE
HHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHC
SGLQVSLCGEMAGDPMGALLLVGLGYRNLSMNGRSVARIKYLLRNIELADAQVLAARVLT
CCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
AQMTTDVRHLTAAFMERRGLGGLIRGGK
HHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLMRLREVVEKVAMATGLTEALELLVNETCLAMDTEVCSIYLADNDRRCYYLMATRGLKK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEECCCEEEEEEECCCCEEEEEEEECCCCC
PRGRTITLAFDEGIVGLVGRLAEPINLADAQSHPSFKYVPQVKEERYRAFLGVPIIYRRQ
CCCCEEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHH
LLGVLVVQQREHRQFDESEESFMVTLATQLAGILSQSQLNAIFGQYRQTRIRALAAAPGV
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AVAEGWQDISQPSLDLVYEASTLDSTLERERLTQALEEAAAEFRRFSKRFAASSQKESAA
HHHHCHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
IFDLYSHLLNDARLKRELFAQIDAGAVAEWAVKQVVEAFAAQFASLQDTYMRERASDLRA
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGQRLLFHLDDSTSGASQWPERFILVADELTATLLAEVPQDRLAGVVVRDGAANSHAAIL
HHHHHEEEECCCCCCCCCCCHHEEEEHHHHHHHHHHHCCHHHHCEEEEECCCCCCCCEEE
VRAMGIPTVMGADIQPALLSQRLLIVDGYRGEVLVDPEPVLVKEYQRLVTEEIELSKLAE
EEECCCCCCCCCCCCHHHHCCCEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHH
DDVEQPAALKSGERIQVMLNAGLSPEHEQLLGGRVDGVGLYRTEIPFMLQSGFPSEEEQV
HHHCCCHHHCCCCEEEEEEECCCCCCHHHHHCCCCCCCEEEECCCCHHHHCCCCCCHHHH
AQYQGMLQLYPQKPVTLRTLDIGADKQLPYMPISEENPCLGWRGIRITLDQPEIFLIQVR
HHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEEHHHHH
AMLRANAGTGNLGILLPMITSIEEVDEAKRLIDRAGREVEEMLGYVLPQPRLGVMIEVPS
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEEECHH
MIFMLPYLTSRVDFISVGTNDLTQYLLAVDRNNTRVASLYDSLHPAMLQVLRQILMQATE
HHHHHHHHHCCCCEEECCHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHC
SGLQVSLCGEMAGDPMGALLLVGLGYRNLSMNGRSVARIKYLLRNIELADAQVLAARVLT
CCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHH
AQMTTDVRHLTAAFMERRGLGGLIRGGK
HHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 7896715; 8973315 [H]