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The map label for this gene is ddl

Identifier: 218927753

GI number: 218927753

Start: 602769

End: 603689

Strand: Direct

Name: ddl

Synonym: YPO0557

Alternate gene names: 218927753

Gene position: 602769-603689 (Clockwise)

Preceding gene: 218927752

Following gene: 218927754

Centisome position: 12.95

GC content: 50.38

Gene sequence:

>921_bases
ATGGCTGAGAAAGTCGCGGTATTGCTGGGTGGGACCTCTGCGGAGCGTGAAGTGTCATTACTGTCAGGTCAGGCGGTATT
GGCAGGCTTGAAAGAAGCCGGTATCGATGCTTATGGGGTGGATACCAAAGATTTTCCGGTAACTCAGCTTAAAGAACAGG
GCTTTGATAAAGTGTTTATCGCCCTGCATGGTCGTGGTGGCGAAGACGGTACGTTACAAGGCGTATTGGAATTTCTACAA
CTGCCATATACCGGCAGTGGTGTAATGGCATCCGCCCTGACAATGGACAAATTGCGCACCAAATTAGTCTGGCAAGCCTT
GGGGTTGCCTATTTCGCCTTATGTTGCGTTGAATCGCCAACAGTTTGAAACACTTTCCCCAGAGGAATTGGTGGCGTGTG
TCGCTAAGCTTGGCCTGCCACTTATTGTCAAGCCAAGCCATGAAGGCTCTAGCGTTGGGATGAGTAAAGTTGATCACGCC
AGCGAATTGCAGAAGGCATTGGTTGAAGCTTTCCAACATGACAGCGATGTACTGATTGAGAAATGGCTGAGCGGGCCAGA
GTTTACTGTCGCGATCCTTGGGGATGAAGTTTTGCCTTCTATTCGTATTCAGCCTCCGGGCGTGTTTTACGATTATGACG
CTAAGTATTTGTCAGATAAAACACAATACTTCTGCCCAAGCGGTTTAAGTGATGAGTCGGAGCAACAATTGGCAGCGCTG
GCATTGCAGGCTTACCACGCGCTGGATTGCAGCGGTTGGGGGCGGGTCGATGTGATGCAAGACCGCGATGGTCATTTTTA
CCTGCTTGAAGTGAACACTTCACCGGGTATGACCAGCCATAGTTTGGTTCCAATGGCCGCCCGCCAGTATGGGTTGAGTT
TCTCCCAATTGGTGGCGCGCATTCTGATGTTGGCTGACTGA

Upstream 100 bases:

>100_bases
GAAGACCTGATCTTGGTTCAGGGCGCAGGTAACATTGGTAAAATTGCCCGTAAATTGGCTGAGCATAAATTGCAGCCACA
GTTAAAAGACGAGGAACATC

Downstream 100 bases:

>100_bases
TATGTCGCAAGCTGCCTTGAATGCGCGAGAGCGTGCGGCGGAGAAAAATGCAGCCCGGCGCAGTAACGGGAGCCAATTGG
CAGGGGTGATTTTTCTGCTG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MAEKVAVLLGGTSAEREVSLLSGQAVLAGLKEAGIDAYGVDTKDFPVTQLKEQGFDKVFIALHGRGGEDGTLQGVLEFLQ
LPYTGSGVMASALTMDKLRTKLVWQALGLPISPYVALNRQQFETLSPEELVACVAKLGLPLIVKPSHEGSSVGMSKVDHA
SELQKALVEAFQHDSDVLIEKWLSGPEFTVAILGDEVLPSIRIQPPGVFYDYDAKYLSDKTQYFCPSGLSDESEQQLAAL
ALQAYHALDCSGWGRVDVMQDRDGHFYLLEVNTSPGMTSHSLVPMAARQYGLSFSQLVARILMLAD

Sequences:

>Translated_306_residues
MAEKVAVLLGGTSAEREVSLLSGQAVLAGLKEAGIDAYGVDTKDFPVTQLKEQGFDKVFIALHGRGGEDGTLQGVLEFLQ
LPYTGSGVMASALTMDKLRTKLVWQALGLPISPYVALNRQQFETLSPEELVACVAKLGLPLIVKPSHEGSSVGMSKVDHA
SELQKALVEAFQHDSDVLIEKWLSGPEFTVAILGDEVLPSIRIQPPGVFYDYDAKYLSDKTQYFCPSGLSDESEQQLAAL
ALQAYHALDCSGWGRVDVMQDRDGHFYLLEVNTSPGMTSHSLVPMAARQYGLSFSQLVARILMLAD
>Mature_305_residues
AEKVAVLLGGTSAEREVSLLSGQAVLAGLKEAGIDAYGVDTKDFPVTQLKEQGFDKVFIALHGRGGEDGTLQGVLEFLQL
PYTGSGVMASALTMDKLRTKLVWQALGLPISPYVALNRQQFETLSPEELVACVAKLGLPLIVKPSHEGSSVGMSKVDHAS
ELQKALVEAFQHDSDVLIEKWLSGPEFTVAILGDEVLPSIRIQPPGVFYDYDAKYLSDKTQYFCPSGLSDESEQQLAALA
LQAYHALDCSGWGRVDVMQDRDGHFYLLEVNTSPGMTSHSLVPMAARQYGLSFSQLVARILMLAD

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=306, Percent_Identity=72.5490196078431, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1786579, Length=349, Percent_Identity=32.9512893982808, Blast_Score=173, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_YERP3 (A7FM64)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001402342.1
- ProteinModelPortal:   A7FM64
- SMR:   A7FM64
- STRING:   A7FM64
- GeneID:   5384314
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_3385
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   EVLVEKW
- ProtClustDB:   PRK01372
- BioCyc:   YPSE349747:YPSIP31758_3385-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 33150; Mature: 33019

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKVAVLLGGTSAEREVSLLSGQAVLAGLKEAGIDAYGVDTKDFPVTQLKEQGFDKVFI
CCCEEEEEEECCCCHHHHHHHCCHHHHHHHHHCCCCEECCCCCCCCHHHHHHCCCCEEEE
ALHGRGGEDGTLQGVLEFLQLPYTGSGVMASALTMDKLRTKLVWQALGLPISPYVALNRQ
EEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECHH
QFETLSPEELVACVAKLGLPLIVKPSHEGSSVGMSKVDHASELQKALVEAFQHDSDVLIE
HHHCCCHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
KWLSGPEFTVAILGDEVLPSIRIQPPGVFYDYDAKYLSDKTQYFCPSGLSDESEQQLAAL
HHCCCCCEEEEEECCCCCCCEEECCCCEEEECCCHHHCCCCCEECCCCCCCHHHHHHHHH
ALQAYHALDCSGWGRVDVMQDRDGHFYLLEVNTSPGMTSHSLVPMAARQYGLSFSQLVAR
HHHHHHHCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHH
ILMLAD
HHHHCC
>Mature Secondary Structure 
AEKVAVLLGGTSAEREVSLLSGQAVLAGLKEAGIDAYGVDTKDFPVTQLKEQGFDKVFI
CCEEEEEEECCCCHHHHHHHCCHHHHHHHHHCCCCEECCCCCCCCHHHHHHCCCCEEEE
ALHGRGGEDGTLQGVLEFLQLPYTGSGVMASALTMDKLRTKLVWQALGLPISPYVALNRQ
EEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECHH
QFETLSPEELVACVAKLGLPLIVKPSHEGSSVGMSKVDHASELQKALVEAFQHDSDVLIE
HHHCCCHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
KWLSGPEFTVAILGDEVLPSIRIQPPGVFYDYDAKYLSDKTQYFCPSGLSDESEQQLAAL
HHCCCCCEEEEEECCCCCCCEEECCCCEEEECCCHHHCCCCCEECCCCCCCHHHHHHHHH
ALQAYHALDCSGWGRVDVMQDRDGHFYLLEVNTSPGMTSHSLVPMAARQYGLSFSQLVAR
HHHHHHHCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHH
ILMLAD
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA