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The map label for this gene is dnaB [H]

Identifier: 218927525

GI number: 218927525

Start: 326497

End: 327903

Strand: Direct

Name: dnaB [H]

Synonym: YPO0320

Alternate gene names: 218927525

Gene position: 326497-327903 (Clockwise)

Preceding gene: 218927523

Following gene: 218927526

Centisome position: 7.02

GC content: 52.1

Gene sequence:

>1407_bases
ATGGCAGCGAAAAAACCAACCAACAAGATGACCGAGCCACGAGACCGCCAGATGGAAGGGCTGAAGCTTCCGCCGCATTC
GCTGGAGGCTGAGCAGTCCGTGTTGGGCGGTTTGATGCTGGATAACGAACGTTGGGATAACGTATCAGAACGTGTCGCCA
GCAAGGACTTTTTCAGCCGCCCGCATCGCCGGATCTTTACTGAAATGCAGCGCTTGTTGGAAAACAGCAAGCCGATCGAC
TTGATCACCTTGTCCGAATCGCTAGAGCAAAAAGGCGATTTAGACTCGGTGGGGGGCTTTGCCTATCTGGCCGAACTATC
TAAAAACACGCCAAGTGCGGCGAATATCGGTGCTTATGCCGATATCGTGCGTGAACGTGCCGTGGTTCGTGAAATGATCT
CTGTGGCGAATGAGATCGCCGATGCCGGCTACGATCCCCAAGGGCGCAGCAGTGAAGATCTGCTGGATTTGGCGGAGTCC
AAAGTTTTCCAAATCGCAGAAAGCCGCGCCAGTAAAGATGAAGGTCCTAAAAGCGTTGATCGCATTCTGGAAGATACCGT
CGCCCGTATTGAACAACTTTATCAGCGCCCACACGATGGCGTCACTGGTGTTTCAACGGGCTTTACTGATCTCGATAAAA
AGACCGCTGGGCTACAAAAATCCGATCTGATCATTGTGGCGGCCCGTCCATCAATGGGGAAAACCACCTTTGCGATGAAC
CTGTGTGAAAACGCGGCGATGATGCAGGACAAACCGGTGTTGATCTTCAGCCTGGAGATGCCCGGCGATCAGATCATGAT
GCGTATGCTGGCATCCTTGTCACATGTCGATCAGACCCGCATCCGTACCGGGCAGTTAGATGATGAGGATTGGGCGCGTA
TTTCCAGCACCATGGGCATATTGATGGAAAAACGCAATATGTACATTGATGACTCCTCGGGTCTGACACCAACGGAAGTC
CGCTCTCGTGCGCGGCGTATCTTCCGTGAACACGGGGGGTTGAGCCTGATCATGATCGACTACCTGCAATTAATGCGGGT
GCCGTCCTTGTCGGATAACCGTACGCTCGAAATCGCGGAAATTTCACGCTCACTGAAAGCCTTGGCGAAAGAGCTGCAAG
TGCCGGTAGTCGCGCTTTCCCAGCTTAACCGTAGTTTGGAGCAGCGTGCCGATAAGCGCCCGGTTAACTCCGACTTGCGT
GAGTCCGGATCGATTGAGCAGGATGCCGACTTAATCATGTTTATTTACCGTGATGAGGTTTATCACGAAAACAGTGATGA
GAAAGGGATCGCACAAATTATTTTGGGTAAACAGCGTAATGGCCCGATCGGTTCCGTTCGTCTGAAATTTAACGGTCAGT
GGTCACGTTTTGATAACTATGCGGGTCCGCAATATGACGACGAGTAG

Upstream 100 bases:

>100_bases
TCGGATATCATCGCGTATACTAGGCCGTTATATTTTTTAGTCGCGCTTTTTCTATTCGTCCTTTTTCTATTTGCAATAAT
AAAGGTAAAGAACACTCTTC

Downstream 100 bases:

>100_bases
TGAATAGCCTCTTGGTATTTGATACTGCCGAGGTGAGCACGGACTACCTGCAACGCCAATCATGTTGGGAATAATATCAA
TTATTAAGGATAAGCAATGA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MAAKKPTNKMTEPRDRQMEGLKLPPHSLEAEQSVLGGLMLDNERWDNVSERVASKDFFSRPHRRIFTEMQRLLENSKPID
LITLSESLEQKGDLDSVGGFAYLAELSKNTPSAANIGAYADIVRERAVVREMISVANEIADAGYDPQGRSSEDLLDLAES
KVFQIAESRASKDEGPKSVDRILEDTVARIEQLYQRPHDGVTGVSTGFTDLDKKTAGLQKSDLIIVAARPSMGKTTFAMN
LCENAAMMQDKPVLIFSLEMPGDQIMMRMLASLSHVDQTRIRTGQLDDEDWARISSTMGILMEKRNMYIDDSSGLTPTEV
RSRARRIFREHGGLSLIMIDYLQLMRVPSLSDNRTLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSDLR
ESGSIEQDADLIMFIYRDEVYHENSDEKGIAQIILGKQRNGPIGSVRLKFNGQWSRFDNYAGPQYDDE

Sequences:

>Translated_468_residues
MAAKKPTNKMTEPRDRQMEGLKLPPHSLEAEQSVLGGLMLDNERWDNVSERVASKDFFSRPHRRIFTEMQRLLENSKPID
LITLSESLEQKGDLDSVGGFAYLAELSKNTPSAANIGAYADIVRERAVVREMISVANEIADAGYDPQGRSSEDLLDLAES
KVFQIAESRASKDEGPKSVDRILEDTVARIEQLYQRPHDGVTGVSTGFTDLDKKTAGLQKSDLIIVAARPSMGKTTFAMN
LCENAAMMQDKPVLIFSLEMPGDQIMMRMLASLSHVDQTRIRTGQLDDEDWARISSTMGILMEKRNMYIDDSSGLTPTEV
RSRARRIFREHGGLSLIMIDYLQLMRVPSLSDNRTLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSDLR
ESGSIEQDADLIMFIYRDEVYHENSDEKGIAQIILGKQRNGPIGSVRLKFNGQWSRFDNYAGPQYDDE
>Mature_467_residues
AAKKPTNKMTEPRDRQMEGLKLPPHSLEAEQSVLGGLMLDNERWDNVSERVASKDFFSRPHRRIFTEMQRLLENSKPIDL
ITLSESLEQKGDLDSVGGFAYLAELSKNTPSAANIGAYADIVRERAVVREMISVANEIADAGYDPQGRSSEDLLDLAESK
VFQIAESRASKDEGPKSVDRILEDTVARIEQLYQRPHDGVTGVSTGFTDLDKKTAGLQKSDLIIVAARPSMGKTTFAMNL
CENAAMMQDKPVLIFSLEMPGDQIMMRMLASLSHVDQTRIRTGQLDDEDWARISSTMGILMEKRNMYIDDSSGLTPTEVR
SRARRIFREHGGLSLIMIDYLQLMRVPSLSDNRTLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSDLRE
SGSIEQDADLIMFIYRDEVYHENSDEKGIAQIILGKQRNGPIGSVRLKFNGQWSRFDNYAGPQYDDE

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=471, Percent_Identity=84.9256900212314, Blast_Score=822, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 52575; Mature: 52444

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKKPTNKMTEPRDRQMEGLKLPPHSLEAEQSVLGGLMLDNERWDNVSERVASKDFFSR
CCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHCCHHCCCCHHCCHHHHHHHHHHHHC
PHRRIFTEMQRLLENSKPIDLITLSESLEQKGDLDSVGGFAYLAELSKNTPSAANIGAYA
HHHHHHHHHHHHHHCCCCEEEEECCHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHH
DIVRERAVVREMISVANEIADAGYDPQGRSSEDLLDLAESKVFQIAESRASKDEGPKSVD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RILEDTVARIEQLYQRPHDGVTGVSTGFTDLDKKTAGLQKSDLIIVAARPSMGKTTFAMN
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCCHHHHHH
LCENAAMMQDKPVLIFSLEMPGDQIMMRMLASLSHVDQTRIRTGQLDDEDWARISSTMGI
HHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LMEKRNMYIDDSSGLTPTEVRSRARRIFREHGGLSLIMIDYLQLMRVPSLSDNRTLEIAE
HHHHCCCEEECCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCEEHHHH
ISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEV
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEEHHH
YHENSDEKGIAQIILGKQRNGPIGSVRLKFNGQWSRFDNYAGPQYDDE
HCCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCHHHHCCCCCCCCCCC
>Mature Secondary Structure 
AAKKPTNKMTEPRDRQMEGLKLPPHSLEAEQSVLGGLMLDNERWDNVSERVASKDFFSR
CCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHCCHHCCCCHHCCHHHHHHHHHHHHC
PHRRIFTEMQRLLENSKPIDLITLSESLEQKGDLDSVGGFAYLAELSKNTPSAANIGAYA
HHHHHHHHHHHHHHCCCCEEEEECCHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHH
DIVRERAVVREMISVANEIADAGYDPQGRSSEDLLDLAESKVFQIAESRASKDEGPKSVD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RILEDTVARIEQLYQRPHDGVTGVSTGFTDLDKKTAGLQKSDLIIVAARPSMGKTTFAMN
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCCHHHHHH
LCENAAMMQDKPVLIFSLEMPGDQIMMRMLASLSHVDQTRIRTGQLDDEDWARISSTMGI
HHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LMEKRNMYIDDSSGLTPTEVRSRARRIFREHGGLSLIMIDYLQLMRVPSLSDNRTLEIAE
HHHHCCCEEECCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCEEHHHH
ISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEV
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCEEEEEEEHHH
YHENSDEKGIAQIILGKQRNGPIGSVRLKFNGQWSRFDNYAGPQYDDE
HCCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]