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The map label for this gene is leuA2

Identifier: 15921567

GI number: 15921567

Start: 1301429

End: 1302811

Strand: Direct

Name: leuA2

Synonym: ST1301

Alternate gene names: 15921567

Gene position: 1301429-1302811 (Clockwise)

Preceding gene: 15921566

Following gene: 15921572

Centisome position: 48.29

GC content: 34.2

Gene sequence:

>1383_bases
ATGAAAGTAGGAATCCTTGACTCAACCCTAAGAGAAGGAGAACAAACACCCGGAGTTGTATTCACAATAGATCAAAGAGT
CGAAATAGCAAAAGCATTATCAGATGTTGGTGTTCAGATGATAGAAGCAGGACATCCTGCGGTATCCTCGGATATTTATG
AGGGAATAAAAAGGATTATGAAACTTAAAAGAGAAGGACTTATTACATCAGAAATAGTAGGGCATAGTAGGGCTGTAAAA
AAAGATATCGAAGTAGCAGCAGAATTAGAAGTAGATAGAATAGCAATATTTTATGGTGTAAGTGATATCCACCTTAAAGC
AAAACATCATGTTACAAGAGAGGAGGCACTTAATATAATAGCAGAAACAATAAGTTATGCAAAAAGTCATGGTGTTAAAG
TAAGATTTACAGCAGAAGACGGTAGCAGAACCGATTTAGATTATTTAATAAAAGTGTGTAAAACAGCAAGAGATGCTGGT
GCTGACAGAGTAAGTATAGCCGATACTGTGGGTATTCTTTATCCCACAAAAACAAGAGAATTATTTAGTACCTTAGTAAG
GGAAGTACCAGGTTTAGAATTTGATATTCATGCTCATAATGATTTAGGTTTAGCCGTTGCTAATGCGTTGGCAGCAATAG
AAGGCGGAGCTACTATAATTCATACTACAGTAAATGGATTAGGAGAAAGAGTAGGAATTGTTCCTCTCCAAGTAATTGCG
GCAGCAATAAAATATCATTTTGGAATTGAAGTTGTAAAGTTAAATAAATTACAACAATTAGCCAGTCTAGTTGAAAAATA
TAGTGGTATACCGATGCCTCCAAACTATCCAATAACAGGAGATTATGCATTTATTCATAAAGCTGGTATTCATGTGGCTG
GAGTTCTTAATGATCCATCAACTTACGAGTTTATGCCACCAGAGACTTTTGGAAGAAGTAGAGACTACGTTATTGATAAA
TATACTGGTAAGCACGCACTAAAAGATAGGTTTGAAAAATTGGGCGTTAAATTAAGTGATGTAGAACTAGATCAAGTACT
AGCTAAAATAAAATCCAATCCAAATGTTAGATTCTATAGAGATGTAGACTTATTAGAAATAGCTGAGTCAGTTACTGGCA
GAGTATTAAAACCTAAACCGCCAGAAAATATTGAAGCTTTAATTTCAGTTAAATGTGAGTCAAATGTTTATACTACAGCA
GTAACTAGAAGATTATCTGTTATTCCAGGAGTAAAAGAAGTCATGGAAATCTCTGGAGATTATGATATTCTCGTTAAAGT
TGAGGCAAAAGATCCTAATGAACTTAATCAAATTATTGAAAATATCAGAGCTGTAAAGGGTGTAAGCTCTACTTTAACAT
CATTAGTTCTTAAAAAGATGTAA

Upstream 100 bases:

>100_bases
TGTAATTCAACTGATAATAAACTCTCTGAAATAGTTTTTTATTCGAACTTTCCTTTATAGCTTTATTTATAAGGGAATTA
CCAAAAGTAAAACTACTAGT

Downstream 100 bases:

>100_bases
ACTTTTACAATAGCTAAGTATAATTTTTATTTTAGTAAATCTTGAAAGTTACACTAGATTTCTTTATAATTAGTTTATTT
TCTTATTAGTAGATAAAATT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MKVGILDSTLREGEQTPGVVFTIDQRVEIAKALSDVGVQMIEAGHPAVSSDIYEGIKRIMKLKREGLITSEIVGHSRAVK
KDIEVAAELEVDRIAIFYGVSDIHLKAKHHVTREEALNIIAETISYAKSHGVKVRFTAEDGSRTDLDYLIKVCKTARDAG
ADRVSIADTVGILYPTKTRELFSTLVREVPGLEFDIHAHNDLGLAVANALAAIEGGATIIHTTVNGLGERVGIVPLQVIA
AAIKYHFGIEVVKLNKLQQLASLVEKYSGIPMPPNYPITGDYAFIHKAGIHVAGVLNDPSTYEFMPPETFGRSRDYVIDK
YTGKHALKDRFEKLGVKLSDVELDQVLAKIKSNPNVRFYRDVDLLEIAESVTGRVLKPKPPENIEALISVKCESNVYTTA
VTRRLSVIPGVKEVMEISGDYDILVKVEAKDPNELNQIIENIRAVKGVSSTLTSLVLKKM

Sequences:

>Translated_460_residues
MKVGILDSTLREGEQTPGVVFTIDQRVEIAKALSDVGVQMIEAGHPAVSSDIYEGIKRIMKLKREGLITSEIVGHSRAVK
KDIEVAAELEVDRIAIFYGVSDIHLKAKHHVTREEALNIIAETISYAKSHGVKVRFTAEDGSRTDLDYLIKVCKTARDAG
ADRVSIADTVGILYPTKTRELFSTLVREVPGLEFDIHAHNDLGLAVANALAAIEGGATIIHTTVNGLGERVGIVPLQVIA
AAIKYHFGIEVVKLNKLQQLASLVEKYSGIPMPPNYPITGDYAFIHKAGIHVAGVLNDPSTYEFMPPETFGRSRDYVIDK
YTGKHALKDRFEKLGVKLSDVELDQVLAKIKSNPNVRFYRDVDLLEIAESVTGRVLKPKPPENIEALISVKCESNVYTTA
VTRRLSVIPGVKEVMEISGDYDILVKVEAKDPNELNQIIENIRAVKGVSSTLTSLVLKKM
>Mature_460_residues
MKVGILDSTLREGEQTPGVVFTIDQRVEIAKALSDVGVQMIEAGHPAVSSDIYEGIKRIMKLKREGLITSEIVGHSRAVK
KDIEVAAELEVDRIAIFYGVSDIHLKAKHHVTREEALNIIAETISYAKSHGVKVRFTAEDGSRTDLDYLIKVCKTARDAG
ADRVSIADTVGILYPTKTRELFSTLVREVPGLEFDIHAHNDLGLAVANALAAIEGGATIIHTTVNGLGERVGIVPLQVIA
AAIKYHFGIEVVKLNKLQQLASLVEKYSGIPMPPNYPITGDYAFIHKAGIHVAGVLNDPSTYEFMPPETFGRSRDYVIDK
YTGKHALKDRFEKLGVKLSDVELDQVLAKIKSNPNVRFYRDVDLLEIAESVTGRVLKPKPPENIEALISVKCESNVYTTA
VTRRLSVIPGVKEVMEISGDYDILVKVEAKDPNELNQIIENIRAVKGVSSTLTSLVLKKM

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=357, Percent_Identity=33.3333333333333, Blast_Score=193, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6320019, Length=385, Percent_Identity=38.4415584415584, Blast_Score=228, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6320071, Length=363, Percent_Identity=39.1184573002755, Blast_Score=227, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6324682, Length=307, Percent_Identity=21.8241042345277, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6324225, Length=307, Percent_Identity=21.4983713355049, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24582381, Length=243, Percent_Identity=23.0452674897119, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU12_SULTO (Q971S5)

Other databases:

- EMBL:   BA000023
- RefSeq:   NP_377236.1
- ProteinModelPortal:   Q971S5
- GeneID:   1459303
- GenomeReviews:   BA000023_GR
- KEGG:   sto:ST1301
- NMPDR:   fig|273063.1.peg.1404
- HOGENOM:   HBG701974
- OMA:   FTAEDAS
- ProtClustDB:   PRK00915
- BioCyc:   STOK273063:ST1301-MONOMER
- BRENDA:   2.3.3.13
- GO:   GO:0005622
- HAMAP:   MF_01025_A
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011008
- InterPro:   IPR011872
- InterPro:   IPR000891
- InterPro:   IPR019887
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR02146

Pfam domain/function: PF01037 AsnC_trans_reg; PF00682 HMGL-like; SSF54909 Dimer_A_B_barrel

EC number: =2.3.3.13

Molecular weight: Translated: 50511; Mature: 50511

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVGILDSTLREGEQTPGVVFTIDQRVEIAKALSDVGVQMIEAGHPAVSSDIYEGIKRIM
CCCCCCHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
KLKREGLITSEIVGHSRAVKKDIEVAAELEVDRIAIFYGVSDIHLKAKHHVTREEALNII
HHHHHCCHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEHHHHCCHHHHHHHH
AETISYAKSHGVKVRFTAEDGSRTDLDYLIKVCKTARDAGADRVSIADTVGILYPTKTRE
HHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEHHHHHEEECCCCHHH
LFSTLVREVPGLEFDIHAHNDLGLAVANALAAIEGGATIIHTTVNGLGERVGIVPLQVIA
HHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHCCCHHHHHHH
AAIKYHFGIEVVKLNKLQQLASLVEKYSGIPMPPNYPITGDYAFIHKAGIHVAGVLNDPS
HHHHHHCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHEEECCCEEEEEECCCC
TYEFMPPETFGRSRDYVIDKYTGKHALKDRFEKLGVKLSDVELDQVLAKIKSNPNVRFYR
CCCCCCCHHCCCCCCCEEECCCCHHHHHHHHHHHCCEEECCHHHHHHHHHHCCCCEEEEE
DVDLLEIAESVTGRVLKPKPPENIEALISVKCESNVYTTAVTRRLSVIPGVKEVMEISGD
CCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCEEHHHHHHHHHHCCCHHHHHHCCCC
YDILVKVEAKDPNELNQIIENIRAVKGVSSTLTSLVLKKM
EEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVGILDSTLREGEQTPGVVFTIDQRVEIAKALSDVGVQMIEAGHPAVSSDIYEGIKRIM
CCCCCCHHHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
KLKREGLITSEIVGHSRAVKKDIEVAAELEVDRIAIFYGVSDIHLKAKHHVTREEALNII
HHHHHCCHHHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEHHHHCCHHHHHHHH
AETISYAKSHGVKVRFTAEDGSRTDLDYLIKVCKTARDAGADRVSIADTVGILYPTKTRE
HHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEHHHHHEEECCCCHHH
LFSTLVREVPGLEFDIHAHNDLGLAVANALAAIEGGATIIHTTVNGLGERVGIVPLQVIA
HHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHCCCHHHHHHH
AAIKYHFGIEVVKLNKLQQLASLVEKYSGIPMPPNYPITGDYAFIHKAGIHVAGVLNDPS
HHHHHHCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHEEECCCEEEEEECCCC
TYEFMPPETFGRSRDYVIDKYTGKHALKDRFEKLGVKLSDVELDQVLAKIKSNPNVRFYR
CCCCCCCHHCCCCCCCEEECCCCHHHHHHHHHHHCCEEECCHHHHHHHHHHCCCCEEEEE
DVDLLEIAESVTGRVLKPKPPENIEALISVKCESNVYTTAVTRRLSVIPGVKEVMEISGD
CCHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCEEHHHHHHHHHHCCCHHHHHHCCCC
YDILVKVEAKDPNELNQIIENIRAVKGVSSTLTSLVLKKM
EEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11572479