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The map label for this gene is thrA [C]

Identifier: 15921504

GI number: 15921504

Start: 1242964

End: 1244307

Strand: Direct

Name: thrA [C]

Synonym: ST1241

Alternate gene names: 15921504

Gene position: 1242964-1244307 (Clockwise)

Preceding gene: 15921503

Following gene: 15921505

Centisome position: 46.13

GC content: 30.36

Gene sequence:

>1344_bases
ATGAAAGTAATTAAAATAGGTGGATCTATTCAAAAAGATGAAAAAGACTATGAATTAATATTAGATAAATTAAAGAAAGA
AATTGATTATAATGATAAAAACATTATTGTAACCTCAGCAATTAAGAACATTACAAATCAGCTGTTAGATATTATAAAAA
ATACAGATAAAGCGATGGATATTGTTACTGATATTTATGATAGACATGTAAGATTACTTTCTAAGTTAACCAATGGAATA
GAGTTTGAGCGTTCTTTTGGAGAAATTTCAAAACTTGCTGATGAGCTTTTTAAAATCGCATGGTCAGTTAGAGTATTAGA
TGAGGTTACTCCTAGGGTAAAAGATTATATATTATCCTTTGGAGAAAGATTTGCAGTAATTTTACTATCCGCATTTTTAA
GATCTAACAATATACAGTCTAGCTATATATTAGATCCTCCCCTTATAACTGATGAAAATTTTGGAGAAGCTAATGTATTA
CTTCAACAATCTAAAGAAAATTTAACTAAAAAAATCGATGGTATTAATGGAAATGTTATCGTAATCCCAGGATTTATTGG
CAAAAGTACACAAAATAAATTTACTACAATAGGACGTGGAGGAAGTGACTATACTGCAACAATAGTAGGAAAAATACTAG
GAGCAAAAGAGGTCAAGTTAATAACTGAAGTCCCTGGAATAATGACTGCTGATCCTAAGAAAATACCACAAGCTAAAACT
ATTCCAAGATTAGCTCTTGAGGAAGCTATTGAACTTTCTCAACTTGGAGCTAAAAAATTACATCCAAGAACATTCGACCC
TATATTTAATACAAATCTAAAAGTAGTAATCGAAGGACTTTATGAGGAAGGCAGTACTGTAATAGAAGGAAACTGTACAA
AAGATGACATTTTAAAAGGAATCGCCACAAGAAATAACCTAGAACTTATAACAATAGAAAGTACTAATATAGTAGGAAAA
ATTGGTTCTGCCGCTACAATAATGTCAGAAGCTAGAAATGCTAATGTAAACCTTATCGCAATTTCTCAACCAGTCTCAGA
AACAGTCATTCAATTAGTAATTGAGTCTCAAGATAAAGATAGATTAATAGAAAGACTAAATAATTTAAATAATTTAATAG
AAGATATACAATCAAGAAAAGTAAGTGCAATTAGTATAGTTGGTTGCGGATTAAGAAACAAGGAAATTTCGGCTAAAGTC
ATTAATAAGGCTTCATCACTAGACCCTATTTTCATATCTAGAGGTCTGAAAGGCGTTAGCTTAACATTTATAGTTGATGA
AAAAGATGAAAATAGTTTAGCCAAAGAACTTCATGAGGTGATCCTGGAGTGGCAGACAAAATAA

Upstream 100 bases:

>100_bases
GGCATGCGCTAAAGGACCCAGATGCGATGATAAAAGCTGAATCACGAAGAATTTTAGTTAACCCAGAACATATAAATGAA
ATAATCTTGAGTGATCTAAA

Downstream 100 bases:

>100_bases
AAGTTTCACTTCTAGGCTCTACTGGGATGGTAGGACAAAAAATGGTAAAAATGTTAGCAAAGCATCCCTATTTAGAGTTA
GTAAAAGTTAGTGCTTCCCC

Product: aspartokinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MKVIKIGGSIQKDEKDYELILDKLKKEIDYNDKNIIVTSAIKNITNQLLDIIKNTDKAMDIVTDIYDRHVRLLSKLTNGI
EFERSFGEISKLADELFKIAWSVRVLDEVTPRVKDYILSFGERFAVILLSAFLRSNNIQSSYILDPPLITDENFGEANVL
LQQSKENLTKKIDGINGNVIVIPGFIGKSTQNKFTTIGRGGSDYTATIVGKILGAKEVKLITEVPGIMTADPKKIPQAKT
IPRLALEEAIELSQLGAKKLHPRTFDPIFNTNLKVVIEGLYEEGSTVIEGNCTKDDILKGIATRNNLELITIESTNIVGK
IGSAATIMSEARNANVNLIAISQPVSETVIQLVIESQDKDRLIERLNNLNNLIEDIQSRKVSAISIVGCGLRNKEISAKV
INKASSLDPIFISRGLKGVSLTFIVDEKDENSLAKELHEVILEWQTK

Sequences:

>Translated_447_residues
MKVIKIGGSIQKDEKDYELILDKLKKEIDYNDKNIIVTSAIKNITNQLLDIIKNTDKAMDIVTDIYDRHVRLLSKLTNGI
EFERSFGEISKLADELFKIAWSVRVLDEVTPRVKDYILSFGERFAVILLSAFLRSNNIQSSYILDPPLITDENFGEANVL
LQQSKENLTKKIDGINGNVIVIPGFIGKSTQNKFTTIGRGGSDYTATIVGKILGAKEVKLITEVPGIMTADPKKIPQAKT
IPRLALEEAIELSQLGAKKLHPRTFDPIFNTNLKVVIEGLYEEGSTVIEGNCTKDDILKGIATRNNLELITIESTNIVGK
IGSAATIMSEARNANVNLIAISQPVSETVIQLVIESQDKDRLIERLNNLNNLIEDIQSRKVSAISIVGCGLRNKEISAKV
INKASSLDPIFISRGLKGVSLTFIVDEKDENSLAKELHEVILEWQTK
>Mature_447_residues
MKVIKIGGSIQKDEKDYELILDKLKKEIDYNDKNIIVTSAIKNITNQLLDIIKNTDKAMDIVTDIYDRHVRLLSKLTNGI
EFERSFGEISKLADELFKIAWSVRVLDEVTPRVKDYILSFGERFAVILLSAFLRSNNIQSSYILDPPLITDENFGEANVL
LQQSKENLTKKIDGINGNVIVIPGFIGKSTQNKFTTIGRGGSDYTATIVGKILGAKEVKLITEVPGIMTADPKKIPQAKT
IPRLALEEAIELSQLGAKKLHPRTFDPIFNTNLKVVIEGLYEEGSTVIEGNCTKDDILKGIATRNNLELITIESTNIVGK
IGSAATIMSEARNANVNLIAISQPVSETVIQLVIESQDKDRLIERLNNLNNLIEDIQSRKVSAISIVGCGLRNKEISAKV
INKASSLDPIFISRGLKGVSLTFIVDEKDENSLAKELHEVILEWQTK

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=470, Percent_Identity=24.468085106383, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1790455, Length=471, Percent_Identity=26.3269639065817, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI1790376, Length=450, Percent_Identity=24.2222222222222, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6320893, Length=389, Percent_Identity=24.1645244215938, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 49570; Mature: 49570

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIKIGGSIQKDEKDYELILDKLKKEIDYNDKNIIVTSAIKNITNQLLDIIKNTDKAMD
CEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHH
IVTDIYDRHVRLLSKLTNGIEFERSFGEISKLADELFKIAWSVRVLDEVTPRVKDYILSF
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
GERFAVILLSAFLRSNNIQSSYILDPPLITDENFGEANVLLQQSKENLTKKIDGINGNVI
HHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEE
VIPGFIGKSTQNKFTTIGRGGSDYTATIVGKILGAKEVKLITEVPGIMTADPKKIPQAKT
EECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHCCCCCC
IPRLALEEAIELSQLGAKKLHPRTFDPIFNTNLKVVIEGLYEEGSTVIEGNCTKDDILKG
CHHHHHHHHHHHHHCCHHHCCCCCCCHHHCCCHHHHHEEHHHCCCEEEECCCCHHHHHHH
IATRNNLELITIESTNIVGKIGSAATIMSEARNANVNLIAISQPVSETVIQLVIESQDKD
HHCCCCEEEEEEECCCEEECCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCHH
RLIERLNNLNNLIEDIQSRKVSAISIVGCGLRNKEISAKVINKASSLDPIFISRGLKGVS
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHCCCCCEE
LTFIVDEKDENSLAKELHEVILEWQTK
EEEEEECCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKVIKIGGSIQKDEKDYELILDKLKKEIDYNDKNIIVTSAIKNITNQLLDIIKNTDKAMD
CEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHH
IVTDIYDRHVRLLSKLTNGIEFERSFGEISKLADELFKIAWSVRVLDEVTPRVKDYILSF
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
GERFAVILLSAFLRSNNIQSSYILDPPLITDENFGEANVLLQQSKENLTKKIDGINGNVI
HHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEE
VIPGFIGKSTQNKFTTIGRGGSDYTATIVGKILGAKEVKLITEVPGIMTADPKKIPQAKT
EECCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHCCCCCC
IPRLALEEAIELSQLGAKKLHPRTFDPIFNTNLKVVIEGLYEEGSTVIEGNCTKDDILKG
CHHHHHHHHHHHHHCCHHHCCCCCCCHHHCCCHHHHHEEHHHCCCEEEECCCCHHHHHHH
IATRNNLELITIESTNIVGKIGSAATIMSEARNANVNLIAISQPVSETVIQLVIESQDKD
HHCCCCEEEEEEECCCEEECCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCHH
RLIERLNNLNNLIEDIQSRKVSAISIVGCGLRNKEISAKVINKASSLDPIFISRGLKGVS
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHCCCCCHHHHHCCCCCEE
LTFIVDEKDENSLAKELHEVILEWQTK
EEEEEECCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]