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The map label for this gene is rsmF [H]

Identifier: 15920491

GI number: 15920491

Start: 317915

End: 318976

Strand: Reverse

Name: rsmF [H]

Synonym: ST0299

Alternate gene names: 15920491

Gene position: 318976-317915 (Counterclockwise)

Preceding gene: 15920496

Following gene: 15920490

Centisome position: 11.84

GC content: 29.57

Gene sequence:

>1062_bases
ATGTTTGGAGAGATTAATTTTATTTTTATTTCTCTCTCAATATGTTTCATGGTTATGACAATTGAAAATTATGTTAAAAA
GTATAATAATTTATTTACAATCTCTCCTAGTTTAAAGGCTATTTCCCTCGCGTCGAAATATAAATTTCTATCTTATATGG
TAGAACGATATATAAAAATTATGGGAGAAGAAGAAACAGAAGATTTTTTATATAGTTGTTCCTTTCCTTTAAAGAAAAGT
GTAAGATGTAATGATCTTCTAATAGAATGCAATAAACTAGAAAAAATAATGGAAAATAAAGGATTTAAACTAGAAAAAGT
TTCATGGTTAAAACATGGATATATAGTAAAACAGTATCCATCTAAGCCTAGTTTAGGTGCGACAATAGAATATTTATCTG
GCTACTATTATATTCAAGGACTAGCATCGATGGTTCCTCCATACGTATTAGATCCAAATGAAAATGATCTTGTCCTTGAT
ATGGCTGCTGCACCGGGGAGCAAAACTACACAACTAGCACAATTAATGAAAAATAAAGGATTAATAGTTGCCGTAGAAAA
ATCTAGGGAAAGAATTAAATCTCTTTACTCAAATGTAAATAGAATGAAAATTAAAAATGTTGTTTTACTTAGGGCAGACG
CAAGAATATTGCGTAAATTAAATATACCATTTAATAAAATTCTGTTAGATGTTCCGTGCAGTGGTGAAGGTCTTATACCA
GAAGATCCTTCAAGAAAAACTAAAACTACTATAGAGGATTTAAAAATATTTTTTCAAAATCAACTAGAACTTATAGATAT
TGCTTATAATATACTAGAACCAGGAGGCATATTAGTGTATTCTAGCTGTAGTATAGCCCCAGAGGAGAACGAAGCTGTTG
TAAATTATATAATAGAAAATTATGGGGCAAAAACAGATAAAATTTATGGTTACCCTGCTACTAGCGGAATTACTGAATTT
AATGGGATTAAATTTGACGAAAGTTTAAGAAATTGCATAAGGTTTTATCCTCATAAAAGTGGTACGGAGGGATTCTTTGT
TTGCAAAATAATAAAGGAGTAA

Upstream 100 bases:

>100_bases
TTTTCTAAAATCTCTTACTGGACTATTATAATATATATCTATTTGATGTTCTTCATTAAATAGTTTGTACTTAATATTTA
ATATTTTTTCCAATTCTTCA

Downstream 100 bases:

>100_bases
TCGTAGAAATAATTTCCATAGATGAAGCAATAAAATATATTAATAGGATAATAAAAATGTATAATTGTATAGAAAATTAT
GATTTTCTTATAAATAAAGA

Product: Sun family protein

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MFGEINFIFISLSICFMVMTIENYVKKYNNLFTISPSLKAISLASKYKFLSYMVERYIKIMGEEETEDFLYSCSFPLKKS
VRCNDLLIECNKLEKIMENKGFKLEKVSWLKHGYIVKQYPSKPSLGATIEYLSGYYYIQGLASMVPPYVLDPNENDLVLD
MAAAPGSKTTQLAQLMKNKGLIVAVEKSRERIKSLYSNVNRMKIKNVVLLRADARILRKLNIPFNKILLDVPCSGEGLIP
EDPSRKTKTTIEDLKIFFQNQLELIDIAYNILEPGGILVYSSCSIAPEENEAVVNYIIENYGAKTDKIYGYPATSGITEF
NGIKFDESLRNCIRFYPHKSGTEGFFVCKIIKE

Sequences:

>Translated_353_residues
MFGEINFIFISLSICFMVMTIENYVKKYNNLFTISPSLKAISLASKYKFLSYMVERYIKIMGEEETEDFLYSCSFPLKKS
VRCNDLLIECNKLEKIMENKGFKLEKVSWLKHGYIVKQYPSKPSLGATIEYLSGYYYIQGLASMVPPYVLDPNENDLVLD
MAAAPGSKTTQLAQLMKNKGLIVAVEKSRERIKSLYSNVNRMKIKNVVLLRADARILRKLNIPFNKILLDVPCSGEGLIP
EDPSRKTKTTIEDLKIFFQNQLELIDIAYNILEPGGILVYSSCSIAPEENEAVVNYIIENYGAKTDKIYGYPATSGITEF
NGIKFDESLRNCIRFYPHKSGTEGFFVCKIIKE
>Mature_353_residues
MFGEINFIFISLSICFMVMTIENYVKKYNNLFTISPSLKAISLASKYKFLSYMVERYIKIMGEEETEDFLYSCSFPLKKS
VRCNDLLIECNKLEKIMENKGFKLEKVSWLKHGYIVKQYPSKPSLGATIEYLSGYYYIQGLASMVPPYVLDPNENDLVLD
MAAAPGSKTTQLAQLMKNKGLIVAVEKSRERIKSLYSNVNRMKIKNVVLLRADARILRKLNIPFNKILLDVPCSGEGLIP
EDPSRKTKTTIEDLKIFFQNQLELIDIAYNILEPGGILVYSSCSIAPEENEAVVNYIIENYGAKTDKIYGYPATSGITEF
NGIKFDESLRNCIRFYPHKSGTEGFFVCKIIKE

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=318, Percent_Identity=36.7924528301887, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI76150623, Length=318, Percent_Identity=36.7924528301887, Blast_Score=196, Evalue=2e-50,
Organism=Homo sapiens, GI301336155, Length=359, Percent_Identity=27.0194986072423, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI39995082, Length=379, Percent_Identity=25.5936675461741, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI270288818, Length=319, Percent_Identity=27.8996865203762, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI8922322, Length=319, Percent_Identity=27.8996865203762, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI23199998, Length=242, Percent_Identity=30.1652892561983, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI270288816, Length=242, Percent_Identity=30.1652892561983, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI32698918, Length=183, Percent_Identity=29.5081967213115, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI11545785, Length=224, Percent_Identity=31.25, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI40316918, Length=196, Percent_Identity=31.1224489795918, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI87081985, Length=291, Percent_Identity=38.1443298969072, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI2367212, Length=223, Percent_Identity=28.2511210762332, Blast_Score=95, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17536757, Length=319, Percent_Identity=36.3636363636364, Blast_Score=186, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI25143471, Length=174, Percent_Identity=32.183908045977, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI71998419, Length=228, Percent_Identity=28.0701754385965, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI115534021, Length=228, Percent_Identity=28.0701754385965, Blast_Score=88, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6324268, Length=315, Percent_Identity=35.8730158730159, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6319447, Length=271, Percent_Identity=29.8892988929889, Blast_Score=116, Evalue=6e-27,
Organism=Drosophila melanogaster, GI22024126, Length=314, Percent_Identity=34.0764331210191, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI21356513, Length=238, Percent_Identity=31.5126050420168, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24668781, Length=238, Percent_Identity=31.5126050420168, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21355201, Length=281, Percent_Identity=27.7580071174377, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI19920494, Length=173, Percent_Identity=28.9017341040462, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR009072
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 40255; Mature: 40255

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGEINFIFISLSICFMVMTIENYVKKYNNLFTISPSLKAISLASKYKFLSYMVERYIKI
CCCCEEEEEHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
MGEEETEDFLYSCSFPLKKSVRCNDLLIECNKLEKIMENKGFKLEKVSWLKHGYIVKQYP
HCCCCCHHHEEECCCCCCCCCCCCHHHEEHHHHHHHHHCCCCEEEHHHHHHCCEEEEECC
SKPSLGATIEYLSGYYYIQGLASMVPPYVLDPNENDLVLDMAAAPGSKTTQLAQLMKNKG
CCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEECCCCCCHHHHHHHHHCCC
LIVAVEKSRERIKSLYSNVNRMKIKNVVLLRADARILRKLNIPFNKILLDVPCSGEGLIP
EEEEEHHHHHHHHHHHCCHHHEEEEEEEEEECCHHHHHHCCCCHHHEEEECCCCCCCCCC
EDPSRKTKTTIEDLKIFFQNQLELIDIAYNILEPGGILVYSSCSIAPEENEAVVNYIIEN
CCCCCHHHHHHHHHHHHHHCCHHHEEHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHH
YGAKTDKIYGYPATSGITEFNGIKFDESLRNCIRFYPHKSGTEGFFVCKIIKE
CCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MFGEINFIFISLSICFMVMTIENYVKKYNNLFTISPSLKAISLASKYKFLSYMVERYIKI
CCCCEEEEEHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
MGEEETEDFLYSCSFPLKKSVRCNDLLIECNKLEKIMENKGFKLEKVSWLKHGYIVKQYP
HCCCCCHHHEEECCCCCCCCCCCCHHHEEHHHHHHHHHCCCCEEEHHHHHHCCEEEEECC
SKPSLGATIEYLSGYYYIQGLASMVPPYVLDPNENDLVLDMAAAPGSKTTQLAQLMKNKG
CCCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEECCCCCCHHHHHHHHHCCC
LIVAVEKSRERIKSLYSNVNRMKIKNVVLLRADARILRKLNIPFNKILLDVPCSGEGLIP
EEEEEHHHHHHHHHHHCCHHHEEEEEEEEEECCHHHHHHCCCCHHHEEEECCCCCCCCCC
EDPSRKTKTTIEDLKIFFQNQLELIDIAYNILEPGGILVYSSCSIAPEENEAVVNYIIEN
CCCCCHHHHHHHHHHHHHHCCHHHEEHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHH
YGAKTDKIYGYPATSGITEFNGIKFDESLRNCIRFYPHKSGTEGFFVCKIIKE
CCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA