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The map label for this gene is ilvI [H]

Identifier: 15920382

GI number: 15920382

Start: 224601

End: 226349

Strand: Reverse

Name: ilvI [H]

Synonym: ST0202

Alternate gene names: 15920382

Gene position: 226349-224601 (Counterclockwise)

Preceding gene: 15920384

Following gene: 15920380

Centisome position: 8.4

GC content: 34.65

Gene sequence:

>1749_bases
GTGGCCGAGAGGTTAAGGCGGCGGGCCGCAGAGTTTATTGCAGATACCCGTTATTTCGCGGGTTCAAGTCCCGCCCCCGG
CTTATTTAAAAAAATTTATACTTTACTTTGGTTGTATAATCTTGTGAGTCAACCCAAAAGAAAAGAGGAGACAATAGGAA
GAGAAACAAAAGGAGACGAAGCGATTGCTTATACTTTTAAAGAGTTAGGAATCAAAGAAATATTTTCACCTTATAATCTT
CCATCATTTTTGGCTGAAAGACTTAAACAATACGAAATTAATATAAATTATGCGTCAAGCGCCAGAGAGGCTGTAATGTT
AGCCGATTCTTATGCGAGGGAATATAATACGCTTGGAGTAGTTTTGCAATCCCCTGGTGTTTATTTAACTGAGGCTATTG
ATATAATAGCCCAAGCATTTATGGACTCTGTACCACTTCTGCTTATTTCAACTCTAAGATCTTATAGGGATACTGGTAGA
AGTAGAATTGCTGAGTTGAAGACCCAAGATGATTTAATGAATATATTAGCTCCTATAACAAAGATGCGTGAAAGAGTAGT
TAGCATAGAAGAAATAATAATAACTATAGAGAAAGGTTATAAGGAAGCATTAAGTAATAGAAATAGGCCAGTTTATATAG
AAATAGCAGAAGATTTATTTAGGTTGAAAGCATATCCTTTATCTCCTGCTGAGCAGAAACCGGAAAAGAAAACACCAGAT
AAGACTACAGTAGCAAAAGTCGCGGAAGTATTATCAAATTCAAAATCACCAATAATTATTGCTGGTTATGGTGTTTTAGC
TTCTAACTCTTTTGATGAGTTAAAAGAACTTGCTGAGTTATTAGATGCACCAGTTATAGCTACTTTTAGGAGCAAAGGAG
TAATACCAGCATCACATCCGCTTTATGCTGGTGAAGGCCTAGGTTTATTTGCTACCGAAGAAGGAGGCAGACTGCTTGAT
GAAGCAGATGTGATATTAGCTTTAGGAACAAGGTTTACACAACTCTCTACAGCCGGCTGGTCAATGAAATTCAAAGGCTA
TCTTATTCATAATAATGTTGATGGTGAAGATATAGGCAAAGTTTTTATTCCACAAATTCCAGTAGTAGCAGATACAGGCT
TATTCTTAAAAGAACTATTAACACAGCTTAAGGCTAAAATTAAGGAGCCAATAAAGAGGGGTTCTGCTGATAAAATAAAG
GTATATAGAAAAATACACATTTTAGATTCTCATGGAGGTTTATGGCCTTATGATGTAGTAAGGACTATTCAGCAATTCAA
GTTTGATAAGATTTTCATAGATCTAACTGCTCCAACAATAGATTTTGTAAGATTGCATATAGAGAAGCCTTTTAGTTGGA
TAGCTAGTGAATCCTTACTAGAAAGAAATATAGCTATTAGTGGTATCGTAAGGGCATCAAACAATAAGATTATAGGAGTT
ACAGACCTACAAGGGGTAATACAAAATCTATCAATCATTCAAAATAGGTTGAACAAGAGCCATGGAATTCTACTAATATT
AAATGATAATGGAAGTACAACAATAGACGCAATCTCATCTGATATTCCAACTATAAGTAGAACTACTTCAAACCTTGATA
TTAAAGACGAAATTCTAGAGAAAAGTCTGGATGCTAAAACAATACAGAGTATTAGCGAGCTAAAAATGGAATTAGAAAAC
TTAGATGAAAATAAATTAAACGTACTAAATATAAAGATTGAACCCGATTACAAATCTGTAATGTTTTAA

Upstream 100 bases:

>100_bases
TTTTTGAAAAAGGAATCACTTTCTCACGCTCTAAAATGCATAAGTTAACATTAAAATATTTTAGCTCGATAGTGTAAAAC
ATGTTTGAATACATGCCGGG

Downstream 100 bases:

>100_bases
TTTTTACTTTTTTGTTCGCTTAATTAGATTATAAGAAACTATATAAGGATAAAAGGAAAATATGGTATGGGCCCGTCGTC
TAGTCTGGTTAGGACGCCAC

Product: acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 582; Mature: 581

Protein sequence:

>582_residues
MAERLRRRAAEFIADTRYFAGSSPAPGLFKKIYTLLWLYNLVSQPKRKEETIGRETKGDEAIAYTFKELGIKEIFSPYNL
PSFLAERLKQYEININYASSAREAVMLADSYAREYNTLGVVLQSPGVYLTEAIDIIAQAFMDSVPLLLISTLRSYRDTGR
SRIAELKTQDDLMNILAPITKMRERVVSIEEIIITIEKGYKEALSNRNRPVYIEIAEDLFRLKAYPLSPAEQKPEKKTPD
KTTVAKVAEVLSNSKSPIIIAGYGVLASNSFDELKELAELLDAPVIATFRSKGVIPASHPLYAGEGLGLFATEEGGRLLD
EADVILALGTRFTQLSTAGWSMKFKGYLIHNNVDGEDIGKVFIPQIPVVADTGLFLKELLTQLKAKIKEPIKRGSADKIK
VYRKIHILDSHGGLWPYDVVRTIQQFKFDKIFIDLTAPTIDFVRLHIEKPFSWIASESLLERNIAISGIVRASNNKIIGV
TDLQGVIQNLSIIQNRLNKSHGILLILNDNGSTTIDAISSDIPTISRTTSNLDIKDEILEKSLDAKTIQSISELKMELEN
LDENKLNVLNIKIEPDYKSVMF

Sequences:

>Translated_582_residues
MAERLRRRAAEFIADTRYFAGSSPAPGLFKKIYTLLWLYNLVSQPKRKEETIGRETKGDEAIAYTFKELGIKEIFSPYNL
PSFLAERLKQYEININYASSAREAVMLADSYAREYNTLGVVLQSPGVYLTEAIDIIAQAFMDSVPLLLISTLRSYRDTGR
SRIAELKTQDDLMNILAPITKMRERVVSIEEIIITIEKGYKEALSNRNRPVYIEIAEDLFRLKAYPLSPAEQKPEKKTPD
KTTVAKVAEVLSNSKSPIIIAGYGVLASNSFDELKELAELLDAPVIATFRSKGVIPASHPLYAGEGLGLFATEEGGRLLD
EADVILALGTRFTQLSTAGWSMKFKGYLIHNNVDGEDIGKVFIPQIPVVADTGLFLKELLTQLKAKIKEPIKRGSADKIK
VYRKIHILDSHGGLWPYDVVRTIQQFKFDKIFIDLTAPTIDFVRLHIEKPFSWIASESLLERNIAISGIVRASNNKIIGV
TDLQGVIQNLSIIQNRLNKSHGILLILNDNGSTTIDAISSDIPTISRTTSNLDIKDEILEKSLDAKTIQSISELKMELEN
LDENKLNVLNIKIEPDYKSVMF
>Mature_581_residues
AERLRRRAAEFIADTRYFAGSSPAPGLFKKIYTLLWLYNLVSQPKRKEETIGRETKGDEAIAYTFKELGIKEIFSPYNLP
SFLAERLKQYEININYASSAREAVMLADSYAREYNTLGVVLQSPGVYLTEAIDIIAQAFMDSVPLLLISTLRSYRDTGRS
RIAELKTQDDLMNILAPITKMRERVVSIEEIIITIEKGYKEALSNRNRPVYIEIAEDLFRLKAYPLSPAEQKPEKKTPDK
TTVAKVAEVLSNSKSPIIIAGYGVLASNSFDELKELAELLDAPVIATFRSKGVIPASHPLYAGEGLGLFATEEGGRLLDE
ADVILALGTRFTQLSTAGWSMKFKGYLIHNNVDGEDIGKVFIPQIPVVADTGLFLKELLTQLKAKIKEPIKRGSADKIKV
YRKIHILDSHGGLWPYDVVRTIQQFKFDKIFIDLTAPTIDFVRLHIEKPFSWIASESLLERNIAISGIVRASNNKIIGVT
DLQGVIQNLSIIQNRLNKSHGILLILNDNGSTTIDAISSDIPTISRTTSNLDIKDEILEKSLDAKTIQSISELKMELENL
DENKLNVLNIKIEPDYKSVMF

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=353, Percent_Identity=23.7960339943343, Blast_Score=83, Evalue=6e-16,
Organism=Escherichia coli, GI87081685, Length=381, Percent_Identity=27.8215223097113, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1790104, Length=338, Percent_Identity=26.9230769230769, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1786717, Length=347, Percent_Identity=26.8011527377522, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788716, Length=354, Percent_Identity=23.728813559322, Blast_Score=62, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6323755, Length=305, Percent_Identity=26.2295081967213, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320123, Length=308, Percent_Identity=25, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323163, Length=297, Percent_Identity=23.9057239057239, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321524, Length=297, Percent_Identity=24.5791245791246, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 65188; Mature: 65057

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERLRRRAAEFIADTRYFAGSSPAPGLFKKIYTLLWLYNLVSQPKRKEETIGRETKGDE
CHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCC
AIAYTFKELGIKEIFSPYNLPSFLAERLKQYEININYASSAREAVMLADSYAREYNTLGV
HHHHHHHHCCHHHHCCCCCCHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHCCEEE
VLQSPGVYLTEAIDIIAQAFMDSVPLLLISTLRSYRDTGRSRIAELKTQDDLMNILAPIT
EEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMRERVVSIEEIIITIEKGYKEALSNRNRPVYIEIAEDLFRLKAYPLSPAEQKPEKKTPD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHCCCCCCCCCCCCCCCCC
KTTVAKVAEVLSNSKSPIIIAGYGVLASNSFDELKELAELLDAPVIATFRSKGVIPASHP
HHHHHHHHHHHCCCCCCEEEEECCHHCCCCHHHHHHHHHHHCCCEEEHHHCCCCCCCCCC
LYAGEGLGLFATEEGGRLLDEADVILALGTRFTQLSTAGWSMKFKGYLIHNNVDGEDIGK
CCCCCCCEEEEECCCCCCHHHCCEEEEECCHHHEEECCCCEEEEEEEEEECCCCCHHHCC
VFIPQIPVVADTGLFLKELLTQLKAKIKEPIKRGSADKIKVYRKIHILDSHGGLWPYDVV
EECCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCHHHHH
RTIQQFKFDKIFIDLTAPTIDFVRLHIEKPFSWIASESLLERNIAISGIVRASNNKIIGV
HHHHHCCCCEEEEEECCCCEEEHEEECCCCHHHHHHHHHHHHCCEEEEEEEECCCEEEEE
TDLQGVIQNLSIIQNRLNKSHGILLILNDNGSTTIDAISSDIPTISRTTSNLDIKDEILE
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHH
KSLDAKTIQSISELKMELENLDENKLNVLNIKIEPDYKSVMF
HCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHCC
>Mature Secondary Structure 
AERLRRRAAEFIADTRYFAGSSPAPGLFKKIYTLLWLYNLVSQPKRKEETIGRETKGDE
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCC
AIAYTFKELGIKEIFSPYNLPSFLAERLKQYEININYASSAREAVMLADSYAREYNTLGV
HHHHHHHHCCHHHHCCCCCCHHHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHHCCEEE
VLQSPGVYLTEAIDIIAQAFMDSVPLLLISTLRSYRDTGRSRIAELKTQDDLMNILAPIT
EEECCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMRERVVSIEEIIITIEKGYKEALSNRNRPVYIEIAEDLFRLKAYPLSPAEQKPEKKTPD
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHCCCCCCCCCCCCCCCCC
KTTVAKVAEVLSNSKSPIIIAGYGVLASNSFDELKELAELLDAPVIATFRSKGVIPASHP
HHHHHHHHHHHCCCCCCEEEEECCHHCCCCHHHHHHHHHHHCCCEEEHHHCCCCCCCCCC
LYAGEGLGLFATEEGGRLLDEADVILALGTRFTQLSTAGWSMKFKGYLIHNNVDGEDIGK
CCCCCCCEEEEECCCCCCHHHCCEEEEECCHHHEEECCCCEEEEEEEEEECCCCCHHHCC
VFIPQIPVVADTGLFLKELLTQLKAKIKEPIKRGSADKIKVYRKIHILDSHGGLWPYDVV
EECCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCHHHHH
RTIQQFKFDKIFIDLTAPTIDFVRLHIEKPFSWIASESLLERNIAISGIVRASNNKIIGV
HHHHHCCCCEEEEEECCCCEEEHEEECCCCHHHHHHHHHHHHCCEEEEEEEECCCEEEEE
TDLQGVIQNLSIIQNRLNKSHGILLILNDNGSTTIDAISSDIPTISRTTSNLDIKDEILE
HHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHH
KSLDAKTIQSISELKMELENLDENKLNVLNIKIEPDYKSVMF
HCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10555290 [H]