Click here to switch to the map view.

The map label for this gene is mtbA [H]

Identifier: 159185241

GI number: 159185241

Start: 2460562

End: 2462406

Strand: Direct

Name: mtbA [H]

Synonym: Atu2492

Alternate gene names: 159185241

Gene position: 2460562-2462406 (Clockwise)

Preceding gene: 15889755

Following gene: 15889759

Centisome position: 86.59

GC content: 60.81

Gene sequence:

>1845_bases
ATGACGGGCGAGGAGAAGAAGGTGATTTTCGCCTCTTCTCTCGGAACAGTCTTCGAATGGTACGATTTTTATCTATACGG
CTCGCTCGCGATCTATATCGGCGCGAACTTCTTCAGCCAATATCCGGAAACAACGCGTAACATCTTCGCGCTTCTGGCTT
TTGCCGCCGGCTTCCTCGTGCGCCCCTTCGGCGCGCTGGTGTTCGGGCGTCTCGGCGACATTGTCGGCCGTAAATACACC
TTCCTGATCACCATTCTCATCATGGGTGTTTCGACCTTCCTCGTCGGTGTCCTGCCCGGTGCATCGCAGATCGGCATCGC
AGCCCCGATCATCCTGATCATCCTGCGCATGCTGCAGGGTCTGGCGCTTGGCGGTGAATATGGCGGTGCTGCAACCTACG
TGGCAGAACACGCGCCCAACGGTCGCAGGGGCTATTACACCTCATGGATCCAGACAACGGCGACACTCGGCCTGTTCCTG
TCGCTGATGGTTATTCTCGGCGTGCAGTTCGCACTCGGCAAAGAAGCCTTCGCAGCCTGGGGCTGGCGCATTCCCTTCCT
CGTTTCCGTTCTGCTGCTTGGCGTTTCGGTCTGGATTCGTCTGAAAATGAACGAATCCCCTGCCTTCAAGAAGATGAAGG
AAGAGGGCAAGACCTCCAAGGCCCCGCTCAGCGAAGCTTTCGGCCAGTGGAAAAACGCCAAGATCGCCCTGCTCGCCCTC
GTCGGCGCCGTCATCGGCCAGGCCGTCGTCTGGTACACCGGCCAGTTCTACGCGCTGTTCTTCCTGCAGAGCATCTTGAA
GGTGGACGGCCAGTCCGCCAACATCATGGTGGCGGCAGCGCTCATCCTCGGCACCGGCTTCTTCGTGCTCTTCGGCTGGC
TTTCCGACAAGATCGGCCGCAAGCCCATCATCATGGCCGGCCTTATCCTCGCCATGCTGACCTACTTCCCGCTGTTCAAG
GCACTGACATGGGCGGGCAACCCGGCACTTGCGCAGGCACAATCCACCGTTCGCGCCACCGTGACTGCGGCTCCGGGCGA
CTGCAAGTTCCAGTTCAACCCGACTGGGACTGCGAAATTCACCACATCCTGCGATATCGCCACCTCGTTCCTGACCCGCA
ACTCCGTCCCCTATGACGTGGTTGCCGGAGCAGCCGGTGAGCCGGCAAGCGTCAAGCTCGGCGACGCCACCATCGCCAGC
TACGACGCCATCGCGGCCGGCGCGGATGCTTCCGCGAAGGACAAGGCATTCCAGAAGCAGATCAACATTGCACTGCATGA
CAGCGGTTACCCGCTGGTACGCGGTGCGGCACAGGTGCCGGACGCGAAACTGGACGCCTTCATTGCCGCAAACCCGGAAC
TCAACCTCAATGCCGATGCCGTTCGCGCCACCGACAAGAAGATGGTTGCGACCGACAAGCTGGTGGCCGACAAGCTGCTG
ACGCCTGCCGAAACGGCCGGTGCGGCGGAAATGGCCGTCTACACCATCGCCGGCGGCGGTGCCTTCACCATGGTGGCCGA
TCCGGCAGCCGTGAACTGGATCGTCATCATCGCGGTCCTGACCGTCCTCGTGATCTATGTGACGATGGTCTATGGCCCGA
TCGCGGCACTGCTGGTCGAACTGTTCCCGACCCGCATCCGCTACTCCGGCATGTCGCTGCCCTATCACATCGGCAACGGC
TGGTTCGGCGGTCTTCTGCCCGCCACCGCCTTTGCGATGAGCGCGGCGAAAGGCGATATCTATTACGGCCTCTGGTATCC
GATCGTCTTTGCAGGCATCACGCTGGTCATCGGCCTGCTGTTCCTGCCGGAAACCAAGGATCGCGACATCCACACCATGG
AGTGA

Upstream 100 bases:

>100_bases
GTCCGAAGGAGAGAGACGGGCTCCGCAATGCGTCGATTGGGACATCTGTGGAGGACAGAGAATGGCAAATATTGCAGCCG
CAAGCGGCGCTGCCCGTCCA

Downstream 100 bases:

>100_bases
GCGAAAGCACCCCCAAACAACCACCCGGCGCCATGGCGCCGGGTTTTTTATTGCCGCCGTACCGCCACCGCCCGAAATGG
CCTTTTCCCGCCTGTCAATC

Product: MFS permease

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 614; Mature: 613

Protein sequence:

>614_residues
MTGEEKKVIFASSLGTVFEWYDFYLYGSLAIYIGANFFSQYPETTRNIFALLAFAAGFLVRPFGALVFGRLGDIVGRKYT
FLITILIMGVSTFLVGVLPGASQIGIAAPIILIILRMLQGLALGGEYGGAATYVAEHAPNGRRGYYTSWIQTTATLGLFL
SLMVILGVQFALGKEAFAAWGWRIPFLVSVLLLGVSVWIRLKMNESPAFKKMKEEGKTSKAPLSEAFGQWKNAKIALLAL
VGAVIGQAVVWYTGQFYALFFLQSILKVDGQSANIMVAAALILGTGFFVLFGWLSDKIGRKPIIMAGLILAMLTYFPLFK
ALTWAGNPALAQAQSTVRATVTAAPGDCKFQFNPTGTAKFTTSCDIATSFLTRNSVPYDVVAGAAGEPASVKLGDATIAS
YDAIAAGADASAKDKAFQKQINIALHDSGYPLVRGAAQVPDAKLDAFIAANPELNLNADAVRATDKKMVATDKLVADKLL
TPAETAGAAEMAVYTIAGGGAFTMVADPAAVNWIVIIAVLTVLVIYVTMVYGPIAALLVELFPTRIRYSGMSLPYHIGNG
WFGGLLPATAFAMSAAKGDIYYGLWYPIVFAGITLVIGLLFLPETKDRDIHTME

Sequences:

>Translated_614_residues
MTGEEKKVIFASSLGTVFEWYDFYLYGSLAIYIGANFFSQYPETTRNIFALLAFAAGFLVRPFGALVFGRLGDIVGRKYT
FLITILIMGVSTFLVGVLPGASQIGIAAPIILIILRMLQGLALGGEYGGAATYVAEHAPNGRRGYYTSWIQTTATLGLFL
SLMVILGVQFALGKEAFAAWGWRIPFLVSVLLLGVSVWIRLKMNESPAFKKMKEEGKTSKAPLSEAFGQWKNAKIALLAL
VGAVIGQAVVWYTGQFYALFFLQSILKVDGQSANIMVAAALILGTGFFVLFGWLSDKIGRKPIIMAGLILAMLTYFPLFK
ALTWAGNPALAQAQSTVRATVTAAPGDCKFQFNPTGTAKFTTSCDIATSFLTRNSVPYDVVAGAAGEPASVKLGDATIAS
YDAIAAGADASAKDKAFQKQINIALHDSGYPLVRGAAQVPDAKLDAFIAANPELNLNADAVRATDKKMVATDKLVADKLL
TPAETAGAAEMAVYTIAGGGAFTMVADPAAVNWIVIIAVLTVLVIYVTMVYGPIAALLVELFPTRIRYSGMSLPYHIGNG
WFGGLLPATAFAMSAAKGDIYYGLWYPIVFAGITLVIGLLFLPETKDRDIHTME
>Mature_613_residues
TGEEKKVIFASSLGTVFEWYDFYLYGSLAIYIGANFFSQYPETTRNIFALLAFAAGFLVRPFGALVFGRLGDIVGRKYTF
LITILIMGVSTFLVGVLPGASQIGIAAPIILIILRMLQGLALGGEYGGAATYVAEHAPNGRRGYYTSWIQTTATLGLFLS
LMVILGVQFALGKEAFAAWGWRIPFLVSVLLLGVSVWIRLKMNESPAFKKMKEEGKTSKAPLSEAFGQWKNAKIALLALV
GAVIGQAVVWYTGQFYALFFLQSILKVDGQSANIMVAAALILGTGFFVLFGWLSDKIGRKPIIMAGLILAMLTYFPLFKA
LTWAGNPALAQAQSTVRATVTAAPGDCKFQFNPTGTAKFTTSCDIATSFLTRNSVPYDVVAGAAGEPASVKLGDATIASY
DAIAAGADASAKDKAFQKQINIALHDSGYPLVRGAAQVPDAKLDAFIAANPELNLNADAVRATDKKMVATDKLVADKLLT
PAETAGAAEMAVYTIAGGGAFTMVADPAAVNWIVIIAVLTVLVIYVTMVYGPIAALLVELFPTRIRYSGMSLPYHIGNGW
FGGLLPATAFAMSAAKGDIYYGLWYPIVFAGITLVIGLLFLPETKDRDIHTME

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=328, Percent_Identity=35.0609756097561, Blast_Score=195, Evalue=6e-51,
Organism=Escherichia coli, GI1788292, Length=331, Percent_Identity=33.8368580060423, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1788942, Length=322, Percent_Identity=33.8509316770186, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1789941, Length=345, Percent_Identity=34.4927536231884, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI87082404, Length=318, Percent_Identity=25.4716981132075, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI87082231, Length=207, Percent_Identity=27.0531400966184, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 65651; Mature: 65519

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS00107 PROTEIN_KINASE_ATP ; PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGEEKKVIFASSLGTVFEWYDFYLYGSLAIYIGANFFSQYPETTRNIFALLAFAAGFLV
CCCCCCCEEEECHHHHHHHHHHHHHHHHHHEEEEHHHHHHCCHHHHHHHHHHHHHHHHHH
RPFGALVFGRLGDIVGRKYTFLITILIMGVSTFLVGVLPGASQIGIAAPIILIILRMLQG
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LALGGEYGGAATYVAEHAPNGRRGYYTSWIQTTATLGLFLSLMVILGVQFALGKEAFAAW
HCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHC
GWRIPFLVSVLLLGVSVWIRLKMNESPAFKKMKEEGKTSKAPLSEAFGQWKNAKIALLAL
CCCHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHH
VGAVIGQAVVWYTGQFYALFFLQSILKVDGQSANIMVAAALILGTGFFVLFGWLSDKIGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
KPIIMAGLILAMLTYFPLFKALTWAGNPALAQAQSTVRATVTAAPGDCKFQFNPTGTAKF
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEEEEECCCCEEEEECCCCCEEE
TTSCDIATSFLTRNSVPYDVVAGAAGEPASVKLGDATIASYDAIAAGADASAKDKAFQKQ
ECCHHHHHHHHHCCCCCHHHEECCCCCCCEEEECCCHHHHHHHHHCCCCCCHHHHHHHHH
INIALHDSGYPLVRGAAQVPDAKLDAFIAANPELNLNADAVRATDKKMVATDKLVADKLL
EEEEEECCCCCCCCCCCCCCCCHHCEEEECCCCCCCCCHHHHCCCCHHHHHHHHHHHHHC
TPAETAGAAEMAVYTIAGGGAFTMVADPAAVNWIVIIAVLTVLVIYVTMVYGPIAALLVE
CCCCCCCCCCEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFPTRIRYSGMSLPYHIGNGWFGGLLPATAFAMSAAKGDIYYGLWYPIVFAGITLVIGLL
HHHHHHEECCCCCCEECCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHH
FLPETKDRDIHTME
HCCCCCCCCCCCCC
>Mature Secondary Structure 
TGEEKKVIFASSLGTVFEWYDFYLYGSLAIYIGANFFSQYPETTRNIFALLAFAAGFLV
CCCCCCEEEECHHHHHHHHHHHHHHHHHHEEEEHHHHHHCCHHHHHHHHHHHHHHHHHH
RPFGALVFGRLGDIVGRKYTFLITILIMGVSTFLVGVLPGASQIGIAAPIILIILRMLQG
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LALGGEYGGAATYVAEHAPNGRRGYYTSWIQTTATLGLFLSLMVILGVQFALGKEAFAAW
HCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHC
GWRIPFLVSVLLLGVSVWIRLKMNESPAFKKMKEEGKTSKAPLSEAFGQWKNAKIALLAL
CCCHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHCCCCCCCHHHHHCCCCCCHHHHHHH
VGAVIGQAVVWYTGQFYALFFLQSILKVDGQSANIMVAAALILGTGFFVLFGWLSDKIGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
KPIIMAGLILAMLTYFPLFKALTWAGNPALAQAQSTVRATVTAAPGDCKFQFNPTGTAKF
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEEEEECCCCEEEEECCCCCEEE
TTSCDIATSFLTRNSVPYDVVAGAAGEPASVKLGDATIASYDAIAAGADASAKDKAFQKQ
ECCHHHHHHHHHCCCCCHHHEECCCCCCCEEEECCCHHHHHHHHHCCCCCCHHHHHHHHH
INIALHDSGYPLVRGAAQVPDAKLDAFIAANPELNLNADAVRATDKKMVATDKLVADKLL
EEEEEECCCCCCCCCCCCCCCCHHCEEEECCCCCCCCCHHHHCCCCHHHHHHHHHHHHHC
TPAETAGAAEMAVYTIAGGGAFTMVADPAAVNWIVIIAVLTVLVIYVTMVYGPIAALLVE
CCCCCCCCCCEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFPTRIRYSGMSLPYHIGNGWFGGLLPATAFAMSAAKGDIYYGLWYPIVFAGITLVIGLL
HHHHHHEECCCCCCEECCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHH
FLPETKDRDIHTME
HCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]