Click here to switch to the map view.

The map label for this gene is pntA [H]

Identifier: 15889759

GI number: 15889759

Start: 2462596

End: 2464170

Strand: Direct

Name: pntA [H]

Synonym: Atu2493

Alternate gene names: 15889759

Gene position: 2462596-2464170 (Clockwise)

Preceding gene: 159185241

Following gene: 15889760

Centisome position: 86.66

GC content: 60.76

Gene sequence:

>1575_bases
TTGAGAATCGGAACGCCTAAGGAATTATATGAGGGCGAAGCGCGCGTCGGCATGACGCCCGACAGCGCGCAGGCCCTGCA
GAAACTCGGATATGATTGCGTGATAGAGGCCGGCGCCGGCAAGGCAGCCGGTTTTTCCGACGACGCCTATCGCGCGGCTG
GGGTCACGGTGGTCGATAGCGCCGACGCGCTCTTCGCCGGATCCGACATCATCGCCAAGGTTCGTCCGCCGGAAACGTCG
GAGATTGATCGCCTGTCTTCGGACAAGACGCTGATCTCGTTCTTTTATCCCGCCCAGAACAAGGACCTTCTGGAGCAGGC
GAAGGAAAAGGGCGCCAACGTCATCGCCATGGACATGGTGCCGCGCATTTCCCGCGCCCAGAAGATGGATGCCCTGTCCT
CCATGGCCAATATCGCCGGTTATCGCGCGGTCATCGAGGCGGGTAACAATTTCGGCCGCTTCTTCACCGGTCAGGTAACA
GCCGCCGGCAAGGTTCCGCCCGCCAAGGTTCTAGTGATCGGTGCCGGCGTCGCCGGTCTTGCCGCCATCGGCACCGCCAC
CTCGCTCGGCGCCATCACCTATGCCTTCGACGTGCGCCCTGAGGTGGCCGAACAGATCGAATCCATGGGCGCTGAATTCG
TCTATCTCGATTTTGCCGATCAGCAGCAGGATGGTGCCGCCACCGGCGGTTATGCCGCTCCCTCCTCGCCGGAATTCCGC
GAGAAGCAGCTTGAGAAATTTCGCGAACTCGCGCCGCAGATCGATATCGTCATCACCACGGCGCTGATCCCAGGCCGTGA
CGCCCCTAAACTGTGGCTCGCCGACATGGTGGCGGCAATGAAGCCGGGTTCTGTCATCATCGACCTTGCTGCCGAGCGCG
GCGGCAATTGCGATCTGACGGTGCCCGACCAGCGTATCGTTTCCGACAACGGCGTCATCGTCATCGGCTACACGGATTTT
CCGAGCCGCATGGCGGCGCAGGCCTCGACGCTCTATTCCACCAACATCCGTCACATGATGACCGATCTGACGCCCGCGAA
GGACGGCAAGCCGGTTCACAACATGGAAGACGATGTCATTCGCGGCGCGACCGTCACCTATCAGGGCGAGATCACTTTCC
CGCCGCCGCCGCCGAAGATTCAGGCCATTGCGGCGCAGAAGCCGAAGGAACGAGTCAAAGAGCTGACGCCTGAAGAAAAA
CACGCGAAAGAACGGGCCGAGTTCAAGGCGCAGACCAAAAGCCAGGTCGGGCTGCTGGTCATTGGTACGGCGGCCCTGCT
TCTCGTCGGTCTTTTTGCACCAGCAAGCTTCATGAGCCACTTCATCGTCTTCGTGCTCGCCTGCTTCATCGGTTTCCAGG
TTATCTGGAACGTCAGCCACTCGCTTCACACACCGCTGATGGCCGTGACGAACGCCATTTCCGGCATCGTCATCATCGGC
GCGCTGCTGCAGATCGGTTCTGGCAACTGGCTGGTGGTGATCCTTGCCTCGCTTTCCGTCCTGATCGCGACGATCAACAT
CGTTGGCGGTTTCCTCGTGACACGGCGCATGCTCGCCATGTTCCAGAAGTCCTGA

Upstream 100 bases:

>100_bases
GCCTGTCAATCCGCCGCAGGTAGAAAAACAGCAATAACAAGACAAAAACGCCCCAAATGGGCTATGACGGCTTATCAGGA
CGGGAAATGGGAGAGCAGTT

Downstream 100 bases:

>100_bases
GCGTGCGGGGATTTAAATAAATGACCATTGGTATCGTTTCCGCGGCCTATGTTGCCGCAGCCGTTCTTTTCATCCTTTCC
CTCGGCGGCCTTTCCGGACA

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha; Pyridine nucleotide transhydrogenase subunit alpha [H]

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MRIGTPKELYEGEARVGMTPDSAQALQKLGYDCVIEAGAGKAAGFSDDAYRAAGVTVVDSADALFAGSDIIAKVRPPETS
EIDRLSSDKTLISFFYPAQNKDLLEQAKEKGANVIAMDMVPRISRAQKMDALSSMANIAGYRAVIEAGNNFGRFFTGQVT
AAGKVPPAKVLVIGAGVAGLAAIGTATSLGAITYAFDVRPEVAEQIESMGAEFVYLDFADQQQDGAATGGYAAPSSPEFR
EKQLEKFRELAPQIDIVITTALIPGRDAPKLWLADMVAAMKPGSVIIDLAAERGGNCDLTVPDQRIVSDNGVIVIGYTDF
PSRMAAQASTLYSTNIRHMMTDLTPAKDGKPVHNMEDDVIRGATVTYQGEITFPPPPPKIQAIAAQKPKERVKELTPEEK
HAKERAEFKAQTKSQVGLLVIGTAALLLVGLFAPASFMSHFIVFVLACFIGFQVIWNVSHSLHTPLMAVTNAISGIVIIG
ALLQIGSGNWLVVILASLSVLIATINIVGGFLVTRRMLAMFQKS

Sequences:

>Translated_524_residues
MRIGTPKELYEGEARVGMTPDSAQALQKLGYDCVIEAGAGKAAGFSDDAYRAAGVTVVDSADALFAGSDIIAKVRPPETS
EIDRLSSDKTLISFFYPAQNKDLLEQAKEKGANVIAMDMVPRISRAQKMDALSSMANIAGYRAVIEAGNNFGRFFTGQVT
AAGKVPPAKVLVIGAGVAGLAAIGTATSLGAITYAFDVRPEVAEQIESMGAEFVYLDFADQQQDGAATGGYAAPSSPEFR
EKQLEKFRELAPQIDIVITTALIPGRDAPKLWLADMVAAMKPGSVIIDLAAERGGNCDLTVPDQRIVSDNGVIVIGYTDF
PSRMAAQASTLYSTNIRHMMTDLTPAKDGKPVHNMEDDVIRGATVTYQGEITFPPPPPKIQAIAAQKPKERVKELTPEEK
HAKERAEFKAQTKSQVGLLVIGTAALLLVGLFAPASFMSHFIVFVLACFIGFQVIWNVSHSLHTPLMAVTNAISGIVIIG
ALLQIGSGNWLVVILASLSVLIATINIVGGFLVTRRMLAMFQKS
>Mature_524_residues
MRIGTPKELYEGEARVGMTPDSAQALQKLGYDCVIEAGAGKAAGFSDDAYRAAGVTVVDSADALFAGSDIIAKVRPPETS
EIDRLSSDKTLISFFYPAQNKDLLEQAKEKGANVIAMDMVPRISRAQKMDALSSMANIAGYRAVIEAGNNFGRFFTGQVT
AAGKVPPAKVLVIGAGVAGLAAIGTATSLGAITYAFDVRPEVAEQIESMGAEFVYLDFADQQQDGAATGGYAAPSSPEFR
EKQLEKFRELAPQIDIVITTALIPGRDAPKLWLADMVAAMKPGSVIIDLAAERGGNCDLTVPDQRIVSDNGVIVIGYTDF
PSRMAAQASTLYSTNIRHMMTDLTPAKDGKPVHNMEDDVIRGATVTYQGEITFPPPPPKIQAIAAQKPKERVKELTPEEK
HAKERAEFKAQTKSQVGLLVIGTAALLLVGLFAPASFMSHFIVFVLACFIGFQVIWNVSHSLHTPLMAVTNAISGIVIIG
ALLQIGSGNWLVVILASLSVLIATINIVGGFLVTRRMLAMFQKS

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=541, Percent_Identity=44.731977818854, Blast_Score=437, Evalue=1e-123,
Organism=Homo sapiens, GI122939153, Length=541, Percent_Identity=44.731977818854, Blast_Score=437, Evalue=1e-123,
Organism=Escherichia coli, GI1787887, Length=524, Percent_Identity=51.9083969465649, Blast_Score=522, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI17550456, Length=532, Percent_Identity=45.8646616541353, Blast_Score=422, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR004571 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 55863; Mature: 55863

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGTPKELYEGEARVGMTPDSAQALQKLGYDCVIEAGAGKAAGFSDDAYRAAGVTVVDS
CCCCCCHHHHCCCCCCCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHCCEEEEEC
ADALFAGSDIIAKVRPPETSEIDRLSSDKTLISFFYPAQNKDLLEQAKEKGANVIAMDMV
CCCEEECCCEEEECCCCCCHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEHHH
PRISRAQKMDALSSMANIAGYRAVIEAGNNFGRFFTGQVTAAGKVPPAKVLVIGAGVAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECEEEECCCCCCCEEEEEECCHHHH
AAIGTATSLGAITYAFDVRPEVAEQIESMGAEFVYLDFADQQQDGAATGGYAAPSSPEFR
HHHHHHHHHCEEEEEEECCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHH
EKQLEKFRELAPQIDIVITTALIPGRDAPKLWLADMVAAMKPGSVIIDLAAERGGNCDLT
HHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCEE
VPDQRIVSDNGVIVIGYTDFPSRMAAQASTLYSTNIRHMMTDLTPAKDGKPVHNMEDDVI
CCCCCEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
RGATVTYQGEITFPPPPPKIQAIAAQKPKERVKELTPEEKHAKERAEFKAQTKSQVGLLV
CCCEEEEEEEEECCCCCCCEEEHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEE
IGTAALLLVGLFAPASFMSHFIVFVLACFIGFQVIWNVSHSLHTPLMAVTNAISGIVIIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ALLQIGSGNWLVVILASLSVLIATINIVGGFLVTRRMLAMFQKS
HHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRIGTPKELYEGEARVGMTPDSAQALQKLGYDCVIEAGAGKAAGFSDDAYRAAGVTVVDS
CCCCCCHHHHCCCCCCCCCCCHHHHHHHCCCEEEEECCCCCCCCCCCCCHHHCCEEEEEC
ADALFAGSDIIAKVRPPETSEIDRLSSDKTLISFFYPAQNKDLLEQAKEKGANVIAMDMV
CCCEEECCCEEEECCCCCCHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEHHH
PRISRAQKMDALSSMANIAGYRAVIEAGNNFGRFFTGQVTAAGKVPPAKVLVIGAGVAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECEEEECCCCCCCEEEEEECCHHHH
AAIGTATSLGAITYAFDVRPEVAEQIESMGAEFVYLDFADQQQDGAATGGYAAPSSPEFR
HHHHHHHHHCEEEEEEECCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHH
EKQLEKFRELAPQIDIVITTALIPGRDAPKLWLADMVAAMKPGSVIIDLAAERGGNCDLT
HHHHHHHHHHCCCEEEEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCEE
VPDQRIVSDNGVIVIGYTDFPSRMAAQASTLYSTNIRHMMTDLTPAKDGKPVHNMEDDVI
CCCCCEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
RGATVTYQGEITFPPPPPKIQAIAAQKPKERVKELTPEEKHAKERAEFKAQTKSQVGLLV
CCCEEEEEEEEECCCCCCCEEEHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEE
IGTAALLLVGLFAPASFMSHFIVFVLACFIGFQVIWNVSHSLHTPLMAVTNAISGIVIIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ALLQIGSGNWLVVILASLSVLIATINIVGGFLVTRRMLAMFQKS
HHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3525165; 1633824; 9097039; 9278503; 1932078 [H]