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The map label for this gene is ttcA

Identifier: 159184950

GI number: 159184950

Start: 1837691

End: 1838626

Strand: Reverse

Name: ttcA

Synonym: Atu1858

Alternate gene names: 159184950

Gene position: 1838626-1837691 (Counterclockwise)

Preceding gene: 15889160

Following gene: 159184949

Centisome position: 64.7

GC content: 59.29

Gene sequence:

>936_bases
ATGAACATTCTCACCAAGATCGATGACTTTGTAGATCAGGCAGGGGCGGACAAGGTCGGGGCAGATCACGGCCCGTCCGA
AGAAAATGGCAGTTCGCATCCCCTGTTCGACAATGCTCCGCGTTCGGTTTCCTTCAACAAGCTGCGCAAGCGACTGTTGC
GCAATGTGCGGCAGGCGTTTGAAGATTTCGGCATGCTGAACGGCCAGAAGCGCTGGCTGGTAGGCCTTTCCGGCGGCAAG
GACAGCTATGGGCTGCTGGCGCTGCTGCTCGATCTGAAATGGCGCGGCCTCTTGCCGGTGGAGCTGGTGGCCTGCAATCT
GGATCAGGGCCAGCCGAATTTTCCCAAACACGTATTGCCGGAGTATCTGGCGAAGATCGGTGTCGCCCATCGCATCGAAT
ATCGCGACACCTATTCAATCGTGAAGGAAAAGGTGCCTTCCGGCGGCACCTATTGTTCGCTCTGTTCGCGGCTTCGTCGC
GGCAATCTCTACCGCATCGCGCGGGAGGAGGGCTGCGACGCGCTGCTGCTCGGCCATCACCGCGAGGATATTCTCGAGAC
CTTCTTCATGAATTTCTTCCATGGTGGCCGGCTGGCCGGCATGCCGGCGAAGCTGTTGAATGACGAAGGCGACCTGATGG
TCATGCGCCCGCTTGCCTATTGCGCCGAAGAGGATATGGCAAAATTTGCAGCCGCTATGGAATTCCCGATCATTCCCTGC
GACCTCTGCGGCTCGCAGGACGGGCTTCAGCGCAATGCCATGAAGGATATGCTGGCCGATATCGAACGGCGCATGCCGGG
CCGCAAGGACGTGATGCTGCGGGCGCTTGCGCATGTGAACCCGTCGCATCTGCTCGACCCGAAGCTTTTTGATTTTTCCG
CGCTTGCTGTCACCGGCGCGTCACCTGAAGAGCGTAGGGAGGCCAGCCCTCCCTGA

Upstream 100 bases:

>100_bases
GCGACCAAAACCGGCTGGTCGGTATTCGGGGCTTGATATTGCCGGCGGATGGGGGCATTGCCTGCATTAAGAACGGCAGT
CGTGCCGGAGCGAGTTGATG

Downstream 100 bases:

>100_bases
TGAAGAAGTGCCCCGCGCCATGAGCCTTTCCGATAAAGATATCGAATTCCTCGTTTCCACCGTCGCCGCTGCCGGCGCAC
AGGAAATCCTGCCCCGCTTC

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MNILTKIDDFVDQAGADKVGADHGPSEENGSSHPLFDNAPRSVSFNKLRKRLLRNVRQAFEDFGMLNGQKRWLVGLSGGK
DSYGLLALLLDLKWRGLLPVELVACNLDQGQPNFPKHVLPEYLAKIGVAHRIEYRDTYSIVKEKVPSGGTYCSLCSRLRR
GNLYRIAREEGCDALLLGHHREDILETFFMNFFHGGRLAGMPAKLLNDEGDLMVMRPLAYCAEEDMAKFAAAMEFPIIPC
DLCGSQDGLQRNAMKDMLADIERRMPGRKDVMLRALAHVNPSHLLDPKLFDFSALAVTGASPEERREASPP

Sequences:

>Translated_311_residues
MNILTKIDDFVDQAGADKVGADHGPSEENGSSHPLFDNAPRSVSFNKLRKRLLRNVRQAFEDFGMLNGQKRWLVGLSGGK
DSYGLLALLLDLKWRGLLPVELVACNLDQGQPNFPKHVLPEYLAKIGVAHRIEYRDTYSIVKEKVPSGGTYCSLCSRLRR
GNLYRIAREEGCDALLLGHHREDILETFFMNFFHGGRLAGMPAKLLNDEGDLMVMRPLAYCAEEDMAKFAAAMEFPIIPC
DLCGSQDGLQRNAMKDMLADIERRMPGRKDVMLRALAHVNPSHLLDPKLFDFSALAVTGASPEERREASPP
>Mature_311_residues
MNILTKIDDFVDQAGADKVGADHGPSEENGSSHPLFDNAPRSVSFNKLRKRLLRNVRQAFEDFGMLNGQKRWLVGLSGGK
DSYGLLALLLDLKWRGLLPVELVACNLDQGQPNFPKHVLPEYLAKIGVAHRIEYRDTYSIVKEKVPSGGTYCSLCSRLRR
GNLYRIAREEGCDALLLGHHREDILETFFMNFFHGGRLAGMPAKLLNDEGDLMVMRPLAYCAEEDMAKFAAAMEFPIIPC
DLCGSQDGLQRNAMKDMLADIERRMPGRKDVMLRALAHVNPSHLLDPKLFDFSALAVTGASPEERREASPP

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32)

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family

Homologues:

Organism=Escherichia coli, GI1787606, Length=253, Percent_Identity=55.7312252964427, Blast_Score=297, Evalue=6e-82,
Organism=Caenorhabditis elegans, GI193211090, Length=221, Percent_Identity=30.316742081448, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17540090, Length=200, Percent_Identity=27.5, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTCA_AGRT5 (Q7CYD2)

Other databases:

- EMBL:   AE007869
- RefSeq:   NP_354840.2
- ProteinModelPortal:   Q7CYD2
- SMR:   Q7CYD2
- STRING:   Q7CYD2
- GeneID:   1133896
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu1858
- eggNOG:   COG0037
- HOGENOM:   HBG347038
- OMA:   FPHAREE
- PhylomeDB:   Q7CYD2
- ProtClustDB:   PRK10696
- GO:   GO:0005737
- HAMAP:   MF_01850
- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF004976

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 34711; Mature: 34711

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILTKIDDFVDQAGADKVGADHGPSEENGSSHPLFDNAPRSVSFNKLRKRLLRNVRQAF
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EDFGMLNGQKRWLVGLSGGKDSYGLLALLLDLKWRGLLPVELVACNLDQGQPNFPKHVLP
HHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHEEECCCCCCCCCCHHHHH
EYLAKIGVAHRIEYRDTYSIVKEKVPSGGTYCSLCSRLRRGNLYRIAREEGCDALLLGHH
HHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEHHHCCCCEEEECCC
REDILETFFMNFFHGGRLAGMPAKLLNDEGDLMVMRPLAYCAEEDMAKFAAAMEFPIIPC
HHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCH
DLCGSQDGLQRNAMKDMLADIERRMPGRKDVMLRALAHVNPSHLLDPKLFDFSALAVTGA
HHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHCCCCCCCCCHHEEEECC
SPEERREASPP
CHHHHCCCCCC
>Mature Secondary Structure
MNILTKIDDFVDQAGADKVGADHGPSEENGSSHPLFDNAPRSVSFNKLRKRLLRNVRQAF
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
EDFGMLNGQKRWLVGLSGGKDSYGLLALLLDLKWRGLLPVELVACNLDQGQPNFPKHVLP
HHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCHHHEEECCCCCCCCCCHHHHH
EYLAKIGVAHRIEYRDTYSIVKEKVPSGGTYCSLCSRLRRGNLYRIAREEGCDALLLGHH
HHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEHHHCCCCEEEECCC
REDILETFFMNFFHGGRLAGMPAKLLNDEGDLMVMRPLAYCAEEDMAKFAAAMEFPIIPC
HHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCH
DLCGSQDGLQRNAMKDMLADIERRMPGRKDVMLRALAHVNPSHLLDPKLFDFSALAVTGA
HHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHCCCCCCCCCHHEEEECC
SPEERREASPP
CHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194