Click here to switch to the map view.

The map label for this gene is pemK [H]

Identifier: 159184541

GI number: 159184541

Start: 929233

End: 929592

Strand: Direct

Name: pemK [H]

Synonym: Atu0940

Alternate gene names: 159184541

Gene position: 929233-929592 (Clockwise)

Preceding gene: 159184540

Following gene: 159184542

Centisome position: 32.7

GC content: 55.28

Gene sequence:

>360_bases
ATGGTCCGCAACCAGATCCCCAAGCGCGGCGACGTTTATCTGGTTGATCTAAACCCTGTCGTAGGCAGCGAAATCAAGGA
CGAACATCGCTGTGTCGTCATCACGCCCAGAGAAATTAACGCGGTCGGACTCTGTCTCGTCGTCCCGGTGACCACCGGCG
GCATGTTTACGCGCAAGGCAGGGCTTGCCGTAAATATATCCGGCCACAAGACAACGGGCGTCGCTTTGTGCAATCAGGTG
AGAAGCATGGATATCGTCGCCCGGGTTGCCCAGAAGAAAGCGAAATATATCGAAACCCTCGATGATGCGACGATCGATGA
AATCGCCGGGCGCGTCATCAGCATGATCGATCCAGCTTGA

Upstream 100 bases:

>100_bases
CGCTGGCCGAATTACTGGATGGTGCAGAGGAAGTGGCAGCTCTAAACGCCAGGGAGCGGGCGTGGGACACAGCGCCTCCT
GTCGGCAAAGAAGCGTTGTG

Downstream 100 bases:

>100_bases
GCGAGGTTTAAACCTCGTCAAACGCTCCGCCTTCACGCGAAGGATTGCGGTCTATCACCCGTTCCTCGGCGTCGTCGGGC
AGGGCCTCGTCGCTTTCCTG

Product: PemK protein

Products: NA

Alternate protein names: Endoribonuclease PemK; Kid toxin protein [H]

Number of amino acids: Translated: 119; Mature: 119

Protein sequence:

>119_residues
MVRNQIPKRGDVYLVDLNPVVGSEIKDEHRCVVITPREINAVGLCLVVPVTTGGMFTRKAGLAVNISGHKTTGVALCNQV
RSMDIVARVAQKKAKYIETLDDATIDEIAGRVISMIDPA

Sequences:

>Translated_119_residues
MVRNQIPKRGDVYLVDLNPVVGSEIKDEHRCVVITPREINAVGLCLVVPVTTGGMFTRKAGLAVNISGHKTTGVALCNQV
RSMDIVARVAQKKAKYIETLDDATIDEIAGRVISMIDPA
>Mature_119_residues
MVRNQIPKRGDVYLVDLNPVVGSEIKDEHRCVVITPREINAVGLCLVVPVTTGGMFTRKAGLAVNISGHKTTGVALCNQV
RSMDIVARVAQKKAKYIETLDDATIDEIAGRVISMIDPA

Specific function: Part of a toxin-antitoxin (TA) module. Probably functions as an mRNA interferase. Responsible for the stable maintenance of the plasmid during cell division by postsegregational killing of plasmid-less daughter cells. Both pemI and PemK proteins bind to t

COG id: COG2337

COG function: function code T; Growth inhibitor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PemK/MazF family [H]

Homologues:

Organism=Escherichia coli, GI1790672, Length=95, Percent_Identity=38.9473684210526, Blast_Score=67, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003477
- InterPro:   IPR011067 [H]

Pfam domain/function: PF02452 PemK [H]

EC number: NA

Molecular weight: Translated: 12857; Mature: 12857

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRNQIPKRGDVYLVDLNPVVGSEIKDEHRCVVITPREINAVGLCLVVPVTTGGMFTRKA
CCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCEEEEEEEEEECCCCCEEECC
GLAVNISGHKTTGVALCNQVRSMDIVARVAQKKAKYIETLDDATIDEIAGRVISMIDPA
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVRNQIPKRGDVYLVDLNPVVGSEIKDEHRCVVITPREINAVGLCLVVPVTTGGMFTRKA
CCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCEEEEEEEEEECCCCCEEECC
GLAVNISGHKTTGVALCNQVRSMDIVARVAQKKAKYIETLDDATIDEIAGRVISMIDPA
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3019092; 3323833; 2832364; 8455570 [H]