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The map label for this gene is rhtB [H]

Identifier: 159184505

GI number: 159184505

Start: 863160

End: 863771

Strand: Direct

Name: rhtB [H]

Synonym: Atu0866

Alternate gene names: 159184505

Gene position: 863160-863771 (Clockwise)

Preceding gene: 15888206

Following gene: 159184507

Centisome position: 30.38

GC content: 61.27

Gene sequence:

>612_bases
ATGACACCCGAATTCCTCATCACATCCTTCATCGTCGCCGCTTCTCCCGGAACTGGCGTGGTCTACACGCTGGCCGCCGG
TCTTTCGCAGGGGGCAAAGGCGAGCATCATCGCGGCTTTCGGCTGCACGCTCGGCATCGTGCCGCATCTGTTGGCCGCCA
TCACCGGCCTTGCCGCCATTCTGCACACCAGTGCGCTGGCCTTCGGCATCGTCAAATATCTGGGGGTCGCCTATCTGCTC
TACATGGCGTGGAACACGCTGCGGGAGAATGGCGCGCTGAAGATCGATGAGACGAGGCAACCGCAAAAACCCGCCCGCGT
CATCGGTGAGGCGATCCTCATCAATCTGCTGAACCCGAAACTGTCGATCTTCTTTTTTGCCTTCCTGCCGCAGTTCATCG
CGCCCGGCGAGCTATCACCGACATGGCGCATGATCGATCTCGGGCTGATCTTCATGGCCCTGACGTTTCTGGTATTTGCG
CTTTACGGCTGTTTTGCCGCCTCGGTCCGGCGGCAGGTCGTGTCGCGTCCAGCGGTTCTCGCATGGTTGAGGCGCAGCTT
CGCCGCCGCTTTCGTGGCGCTGGGGGCGAAGCTCGCCTTTACCGAGCGTTGA

Upstream 100 bases:

>100_bases
GCGATGGCCTCCGAGCACCAATGCCGCAATTTCATCCAAGACACCCACTCCACGCATCATCATGCTCTCGTCCTCAATCC
CAAGAGGACAGACCCACACC

Downstream 100 bases:

>100_bases
GACCGGACCGGATGGCCGGCCGCCTCAGTCGTCCCAATCGGGCGCGAGGTGGCCGGGGCTGACGATGCGGCCATTCGGCT
TGGCAAGCGCATGGATCGCC

Product: RhtB family transporter

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MTPEFLITSFIVAASPGTGVVYTLAAGLSQGAKASIIAAFGCTLGIVPHLLAAITGLAAILHTSALAFGIVKYLGVAYLL
YMAWNTLRENGALKIDETRQPQKPARVIGEAILINLLNPKLSIFFFAFLPQFIAPGELSPTWRMIDLGLIFMALTFLVFA
LYGCFAASVRRQVVSRPAVLAWLRRSFAAAFVALGAKLAFTER

Sequences:

>Translated_203_residues
MTPEFLITSFIVAASPGTGVVYTLAAGLSQGAKASIIAAFGCTLGIVPHLLAAITGLAAILHTSALAFGIVKYLGVAYLL
YMAWNTLRENGALKIDETRQPQKPARVIGEAILINLLNPKLSIFFFAFLPQFIAPGELSPTWRMIDLGLIFMALTFLVFA
LYGCFAASVRRQVVSRPAVLAWLRRSFAAAFVALGAKLAFTER
>Mature_202_residues
TPEFLITSFIVAASPGTGVVYTLAAGLSQGAKASIIAAFGCTLGIVPHLLAAITGLAAILHTSALAFGIVKYLGVAYLLY
MAWNTLRENGALKIDETRQPQKPARVIGEAILINLLNPKLSIFFFAFLPQFIAPGELSPTWRMIDLGLIFMALTFLVFAL
YGCFAASVRRQVVSRPAVLAWLRRSFAAAFVALGAKLAFTER

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=195, Percent_Identity=28.2051282051282, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788099, Length=134, Percent_Identity=32.8358208955224, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 21823; Mature: 21692

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPEFLITSFIVAASPGTGVVYTLAAGLSQGAKASIIAAFGCTLGIVPHLLAAITGLAAI
CCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHTSALAFGIVKYLGVAYLLYMAWNTLRENGALKIDETRQPQKPARVIGEAILINLLNPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHCHH
LSIFFFAFLPQFIAPGELSPTWRMIDLGLIFMALTFLVFALYGCFAASVRRQVVSRPAVL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
AWLRRSFAAAFVALGAKLAFTER
HHHHHHHHHHHHHHCCHHHCCCC
>Mature Secondary Structure 
TPEFLITSFIVAASPGTGVVYTLAAGLSQGAKASIIAAFGCTLGIVPHLLAAITGLAAI
CHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHTSALAFGIVKYLGVAYLLYMAWNTLRENGALKIDETRQPQKPARVIGEAILINLLNPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHCHH
LSIFFFAFLPQFIAPGELSPTWRMIDLGLIFMALTFLVFALYGCFAASVRRQVVSRPAVL
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
AWLRRSFAAAFVALGAKLAFTER
HHHHHHHHHHHHHHCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]