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The map label for this gene is trpS

Identifier: 159184271

GI number: 159184271

Start: 344570

End: 345634

Strand: Direct

Name: trpS

Synonym: Atu0349

Alternate gene names: 159184271

Gene position: 344570-345634 (Clockwise)

Preceding gene: 15887699

Following gene: 15887701

Centisome position: 12.13

GC content: 59.91

Gene sequence:

>1065_bases
ATGAACGCATTCAAGCCGCTGGTTTTCTCGGGCGTCCAGCCGACCGGCAATCTGCATCTCGGCAATTATCTCGGCGCGAT
CCGCAAATTCGTGGCGCTGCAGGAAGATAACGACTGCATCTATTGCGTCGTCGACATGCACGCCATCACCGCCCAGCTCG
TTCACTCGGACCTGAAGGCGCAGACCCGCTCGATTGCGGCCGCCTTCATCGCAGCCGGCATCGATCCCGTGAAGCATATC
GTCTTCAACCAGTCCGCGGTGCCGCAGCATGCGGAACTGGCATGGGTCTTCAACTGTGTGGCGCGCATCGGCTGGATGGA
GCGCATGACCCAGTTCAAGGACAAGTCCGGCAAGAATGCCGAACAGGTCTCGCTCGGCCTGCTCGCCTATCCGAGCCTGA
TGGCTGCCGACATTCTCGTCTATCGCGCCACGCATGTTCCCGTCGGCGATGACCAGAAACAGCACCTCGAACTCGCCCGC
GATATCGCCCAGAAATTCAACATCGATTTCGGCGGCCATATTCGCAACGCCGGTCTGGGCGTTAATATCACGGTTGGCGA
TGAGCCGGTGCACGCTTATTTCCCGATGGTGGAGCCGCTGATCGGTGGCCCGGCGCCGCGCGTCATGTCGCTGAAGGACG
GCACCAAGAAGATGTCGAAGTCCGACCCTTCCGATCTGTCGCGCATCAATCTGATGGACGATGTCGACGCGATCTCTAAG
AAGATCAAGAAGGCGAAGACCGATCCGGACGCGCTGCCGAGCGAAGTGGAAGGCCTGAAGGGCCGCCCCGAGGCCGAAAA
CCTCGTCGGTATCTATGCTGCGCTCTCCGACAAGACCAAGGCGGATGTTCTTGCCGAATTCGGCGGTCAGCAGTTCTCCA
CCTTCAAGCCGGCCCTCGTGGAGCTTGCCGTCAATGTGCTGGCGCCGGTAAACAACGAGATGCGCCGTCTTCTCGATGAT
CCGACCCATATTGACGCCATCCTCAGCCAGGGCGGCGAGCGGGCACGGACTATCGCTGAAAAGACGATGAACGAGGTGCG
CGATATCATCGGTTTCTTGCGCTGA

Upstream 100 bases:

>100_bases
CCCTTGAAACCGCTTGATTGCGAGGGGCTGTGGGTGCATAAGCGCGCGTGCAATTGCAAACACGGGGCGGGGCCCTCCAC
AAGCCTTTTCGAGGACGACA

Downstream 100 bases:

>100_bases
CAGGCCGTTGCCACGCGAATGCGGCGGGCGGTTTGCGCCTGCCGTGATTTGATATAATCTCCGCCCGAACCGTCGGGCGG
TTTTTCGGATCACGGCGGAT

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MNAFKPLVFSGVQPTGNLHLGNYLGAIRKFVALQEDNDCIYCVVDMHAITAQLVHSDLKAQTRSIAAAFIAAGIDPVKHI
VFNQSAVPQHAELAWVFNCVARIGWMERMTQFKDKSGKNAEQVSLGLLAYPSLMAADILVYRATHVPVGDDQKQHLELAR
DIAQKFNIDFGGHIRNAGLGVNITVGDEPVHAYFPMVEPLIGGPAPRVMSLKDGTKKMSKSDPSDLSRINLMDDVDAISK
KIKKAKTDPDALPSEVEGLKGRPEAENLVGIYAALSDKTKADVLAEFGGQQFSTFKPALVELAVNVLAPVNNEMRRLLDD
PTHIDAILSQGGERARTIAEKTMNEVRDIIGFLR

Sequences:

>Translated_354_residues
MNAFKPLVFSGVQPTGNLHLGNYLGAIRKFVALQEDNDCIYCVVDMHAITAQLVHSDLKAQTRSIAAAFIAAGIDPVKHI
VFNQSAVPQHAELAWVFNCVARIGWMERMTQFKDKSGKNAEQVSLGLLAYPSLMAADILVYRATHVPVGDDQKQHLELAR
DIAQKFNIDFGGHIRNAGLGVNITVGDEPVHAYFPMVEPLIGGPAPRVMSLKDGTKKMSKSDPSDLSRINLMDDVDAISK
KIKKAKTDPDALPSEVEGLKGRPEAENLVGIYAALSDKTKADVLAEFGGQQFSTFKPALVELAVNVLAPVNNEMRRLLDD
PTHIDAILSQGGERARTIAEKTMNEVRDIIGFLR
>Mature_354_residues
MNAFKPLVFSGVQPTGNLHLGNYLGAIRKFVALQEDNDCIYCVVDMHAITAQLVHSDLKAQTRSIAAAFIAAGIDPVKHI
VFNQSAVPQHAELAWVFNCVARIGWMERMTQFKDKSGKNAEQVSLGLLAYPSLMAADILVYRATHVPVGDDQKQHLELAR
DIAQKFNIDFGGHIRNAGLGVNITVGDEPVHAYFPMVEPLIGGPAPRVMSLKDGTKKMSKSDPSDLSRINLMDDVDAISK
KIKKAKTDPDALPSEVEGLKGRPEAENLVGIYAALSDKTKADVLAEFGGQQFSTFKPALVELAVNVLAPVNNEMRRLLDD
PTHIDAILSQGGERARTIAEKTMNEVRDIIGFLR

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7710154, Length=351, Percent_Identity=37.037037037037, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI41352700, Length=168, Percent_Identity=47.6190476190476, Blast_Score=172, Evalue=4e-43,
Organism=Escherichia coli, GI1789786, Length=349, Percent_Identity=40.4011461318052, Blast_Score=275, Evalue=4e-75,
Organism=Caenorhabditis elegans, GI71982800, Length=353, Percent_Identity=33.4277620396601, Blast_Score=193, Evalue=9e-50,
Organism=Caenorhabditis elegans, GI71982793, Length=353, Percent_Identity=33.4277620396601, Blast_Score=192, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6320474, Length=364, Percent_Identity=36.5384615384615, Blast_Score=215, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24666151, Length=348, Percent_Identity=37.9310344827586, Blast_Score=224, Evalue=6e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYW_AGRT5 (Q8UIE8)

Other databases:

- EMBL:   AE007869
- PIR:   AE2619
- PIR:   E97401
- RefSeq:   NP_353381.2
- ProteinModelPortal:   Q8UIE8
- SMR:   Q8UIE8
- STRING:   Q8UIE8
- GeneID:   1132387
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0349
- eggNOG:   COG0180
- HOGENOM:   HBG293263
- OMA:   NKPGVSN
- PhylomeDB:   Q8UIE8
- ProtClustDB:   PRK00927
- BioCyc:   ATUM176299-1:ATU0349-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00140_B
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10055
- PRINTS:   PR01039
- TIGRFAMs:   TIGR00233

Pfam domain/function: PF00579 tRNA-synt_1b

EC number: =6.1.1.2

Molecular weight: Translated: 38648; Mature: 38648

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 220-220

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAFKPLVFSGVQPTGNLHLGNYLGAIRKFVALQEDNDCIYCVVDMHAITAQLVHSDLKA
CCCCCCHHHCCCCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHH
QTRSIAAAFIAAGIDPVKHIVFNQSAVPQHAELAWVFNCVARIGWMERMTQFKDKSGKNA
HHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
EQVSLGLLAYPSLMAADILVYRATHVPVGDDQKQHLELARDIAQKFNIDFGGHIRNAGLG
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCE
VNITVGDEPVHAYFPMVEPLIGGPAPRVMSLKDGTKKMSKSDPSDLSRINLMDDVDAISK
EEEEECCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHH
KIKKAKTDPDALPSEVEGLKGRPEAENLVGIYAALSDKTKADVLAEFGGQQFSTFKPALV
HHHHCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHH
ELAVNVLAPVNNEMRRLLDDPTHIDAILSQGGERARTIAEKTMNEVRDIIGFLR
HHHHHHHHCCCHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNAFKPLVFSGVQPTGNLHLGNYLGAIRKFVALQEDNDCIYCVVDMHAITAQLVHSDLKA
CCCCCCHHHCCCCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHH
QTRSIAAAFIAAGIDPVKHIVFNQSAVPQHAELAWVFNCVARIGWMERMTQFKDKSGKNA
HHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
EQVSLGLLAYPSLMAADILVYRATHVPVGDDQKQHLELARDIAQKFNIDFGGHIRNAGLG
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCE
VNITVGDEPVHAYFPMVEPLIGGPAPRVMSLKDGTKKMSKSDPSDLSRINLMDDVDAISK
EEEEECCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHH
KIKKAKTDPDALPSEVEGLKGRPEAENLVGIYAALSDKTKADVLAEFGGQQFSTFKPALV
HHHHCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHH
ELAVNVLAPVNNEMRRLLDDPTHIDAILSQGGERARTIAEKTMNEVRDIIGFLR
HHHHHHHHCCCHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194