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The map label for this gene is pheS [H]

Identifier: 159184234

GI number: 159184234

Start: 254470

End: 255519

Strand: Direct

Name: pheS [H]

Synonym: Atu0258

Alternate gene names: 159184234

Gene position: 254470-255519 (Clockwise)

Preceding gene: 159184233

Following gene: 15887609

Centisome position: 8.96

GC content: 61.05

Gene sequence:

>1050_bases
ATGTCGGAGATCGCCGCCGCTGCCGATGAGCCGGCCATCGAGGCCGTGCGCGTCTCCGCGCTCGGCAAGAAGGGCTCGGT
TTCGGAGCTTCTGAAGACGCTTGGCAGCATGACGCCGGAGGAGCGCCAGACCCGTGGCGCTGCCATCAACCAGCTGAAGA
CCGAAATCACCGACCTTATCGGCGAGCGCAAGAACGCGCTGAAGGATGCGGCCATCGCCGCCCGGCTGAAGGCGGAAACG
GTGGATGTCAGCCTGCCGGTGCGCCAGTCGCCTACCGAACGTGGCCGTATCCATCCGATCAGCCAGATCGTCGATGAAAT
CACCGCCATCTTCGCGGATATGGGTTTCTCGATCGCCGAAGGTCCTGACATCGAGACCGACTATTACAACTTCACGGCAC
TGAATTTCCCTGAAGGCCATCCGGCACGCGAGATGCACGACACCTTCTTCTTCCAGCCGGACGAAAAAGGCGAGCGCAAG
GTGCTGCGCACCCACACCTCGCCGGTGCAGATCCGCACCATGGAAAGCCAGAAGCCGCCGATCCGCATCGTCATTCCTGG
CAAGACCTATCGTCAGGATTCCGATGCCACCCATTCGCCGATGTTCCATCAGGTTGAGGGTCTTGTCATCGACAAGAAGG
CGCATGTCGGCAATCTGCGCTGGGTGCTGGAAGAGTTCTGCAAGACCTTCTTCGAGGTCGATAGCGTCGTCATGCGCTTC
CGCCCGTCTTTCTTCCCCTTCACGGAACCGAGCTTCGAGGTGGACATCCAGTGCGACCGTTCCGGCCCCATCGTCAAGTT
CGGCGAAGGCAAGGACTGGATGGAAATCCTCGGCTGCGGCATGGTGCACCCCAATGTGCTGCGCGCCGGCGGACTGGATC
CGGATGAATATCAGGGCTTTGCCTGGGGCATGGGCCTCGACCGCATCGCCATGCTGAAATACGGCATGCCGGACCTGCGC
GATTTCTTCAACGCCGATGTCCGCTGGATGAACCATTACGGCTTCCGCCCGCTCGACATGCCGACGCTGTTCGGCGGTCT
GAGCGTTTAA

Upstream 100 bases:

>100_bases
ACCGACTATCTGATGAATGCTGTGAACACCGACGCCGCTGTTAAAAAGTCCGGCAAGGCAGGAAAAGATGACTGAACTTG
ATACCTTGAAATCGCAACTG

Downstream 100 bases:

>100_bases
TCCGGTTAGCCAAGGAGCAACGACATGAAATTCACTCTTTCCTGGCTGAAAGAGCATCTCGAAACGGATGCGACTCTGGA
CGAGATTTGCGAACGGCTGA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MSEIAAAADEPAIEAVRVSALGKKGSVSELLKTLGSMTPEERQTRGAAINQLKTEITDLIGERKNALKDAAIAARLKAET
VDVSLPVRQSPTERGRIHPISQIVDEITAIFADMGFSIAEGPDIETDYYNFTALNFPEGHPAREMHDTFFFQPDEKGERK
VLRTHTSPVQIRTMESQKPPIRIVIPGKTYRQDSDATHSPMFHQVEGLVIDKKAHVGNLRWVLEEFCKTFFEVDSVVMRF
RPSFFPFTEPSFEVDIQCDRSGPIVKFGEGKDWMEILGCGMVHPNVLRAGGLDPDEYQGFAWGMGLDRIAMLKYGMPDLR
DFFNADVRWMNHYGFRPLDMPTLFGGLSV

Sequences:

>Translated_349_residues
MSEIAAAADEPAIEAVRVSALGKKGSVSELLKTLGSMTPEERQTRGAAINQLKTEITDLIGERKNALKDAAIAARLKAET
VDVSLPVRQSPTERGRIHPISQIVDEITAIFADMGFSIAEGPDIETDYYNFTALNFPEGHPAREMHDTFFFQPDEKGERK
VLRTHTSPVQIRTMESQKPPIRIVIPGKTYRQDSDATHSPMFHQVEGLVIDKKAHVGNLRWVLEEFCKTFFEVDSVVMRF
RPSFFPFTEPSFEVDIQCDRSGPIVKFGEGKDWMEILGCGMVHPNVLRAGGLDPDEYQGFAWGMGLDRIAMLKYGMPDLR
DFFNADVRWMNHYGFRPLDMPTLFGGLSV
>Mature_348_residues
SEIAAAADEPAIEAVRVSALGKKGSVSELLKTLGSMTPEERQTRGAAINQLKTEITDLIGERKNALKDAAIAARLKAETV
DVSLPVRQSPTERGRIHPISQIVDEITAIFADMGFSIAEGPDIETDYYNFTALNFPEGHPAREMHDTFFFQPDEKGERKV
LRTHTSPVQIRTMESQKPPIRIVIPGKTYRQDSDATHSPMFHQVEGLVIDKKAHVGNLRWVLEEFCKTFFEVDSVVMRFR
PSFFPFTEPSFEVDIQCDRSGPIVKFGEGKDWMEILGCGMVHPNVLRAGGLDPDEYQGFAWGMGLDRIAMLKYGMPDLRD
FFNADVRWMNHYGFRPLDMPTLFGGLSV

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758340, Length=277, Percent_Identity=31.4079422382672, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI5729820, Length=261, Percent_Identity=26.8199233716475, Blast_Score=96, Evalue=7e-20,
Organism=Escherichia coli, GI1788007, Length=332, Percent_Identity=49.6987951807229, Blast_Score=345, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI32563657, Length=274, Percent_Identity=31.021897810219, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17508957, Length=274, Percent_Identity=31.021897810219, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32566635, Length=281, Percent_Identity=29.5373665480427, Blast_Score=112, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6321087, Length=322, Percent_Identity=29.1925465838509, Blast_Score=131, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6325304, Length=97, Percent_Identity=35.0515463917526, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI18858079, Length=280, Percent_Identity=31.4285714285714, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI17137424, Length=298, Percent_Identity=24.1610738255034, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 39185; Mature: 39054

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIAAAADEPAIEAVRVSALGKKGSVSELLKTLGSMTPEERQTRGAAINQLKTEITDLI
CCCHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
GERKNALKDAAIAARLKAETVDVSLPVRQSPTERGRIHPISQIVDEITAIFADMGFSIAE
HHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
GPDIETDYYNFTALNFPEGHPAREMHDTFFFQPDEKGERKVLRTHTSPVQIRTMESQKPP
CCCCCCCCEEEEEEECCCCCCHHHHHHHHEECCCCCCCHHHHHCCCCCEEEEEECCCCCC
IRIVIPGKTYRQDSDATHSPMFHQVEGLVIDKKAHVGNLRWVLEEFCKTFFEVDSVVMRF
EEEEECCCCCCCCCCCCCCCHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RPSFFPFTEPSFEVDIQCDRSGPIVKFGEGKDWMEILGCGMVHPNVLRAGGLDPDEYQGF
CCCCCCCCCCCEEEEEEECCCCCEEEECCCCHHHHHHHCCCCCCCHHHCCCCCHHHCCCE
AWGMGLDRIAMLKYGMPDLRDFFNADVRWMNHYGFRPLDMPTLFGGLSV
EECCCHHHHHHHHCCCHHHHHHHCCCHHHHHHCCCCCCCCHHHHCCCCC
>Mature Secondary Structure 
SEIAAAADEPAIEAVRVSALGKKGSVSELLKTLGSMTPEERQTRGAAINQLKTEITDLI
CCHHCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
GERKNALKDAAIAARLKAETVDVSLPVRQSPTERGRIHPISQIVDEITAIFADMGFSIAE
HHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
GPDIETDYYNFTALNFPEGHPAREMHDTFFFQPDEKGERKVLRTHTSPVQIRTMESQKPP
CCCCCCCCEEEEEEECCCCCCHHHHHHHHEECCCCCCCHHHHHCCCCCEEEEEECCCCCC
IRIVIPGKTYRQDSDATHSPMFHQVEGLVIDKKAHVGNLRWVLEEFCKTFFEVDSVVMRF
EEEEECCCCCCCCCCCCCCCHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RPSFFPFTEPSFEVDIQCDRSGPIVKFGEGKDWMEILGCGMVHPNVLRAGGLDPDEYQGF
CCCCCCCCCCCEEEEEEECCCCCEEEECCCCHHHHHHHCCCCCCCHHHCCCCCHHHCCCE
AWGMGLDRIAMLKYGMPDLRDFFNADVRWMNHYGFRPLDMPTLFGGLSV
EECCCHHHHHHHHCCCHHHHHHHCCCHHHHHHCCCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA