Click here to switch to the map view.

The map label for this gene is guaA [H]

Identifier: 159184223

GI number: 159184223

Start: 232091

End: 232765

Strand: Direct

Name: guaA [H]

Synonym: Atu0231

Alternate gene names: 159184223

Gene position: 232091-232765 (Clockwise)

Preceding gene: 159184221

Following gene: 15887582

Centisome position: 8.17

GC content: 60.0

Gene sequence:

>675_bases
ATGGCGGGCACGCCGCATGGACAAATTGGCGTCGCCCTGCGAGAGGCGGCGGCGGATATTCACATCATCCGCGCCTATGC
GGGCGATCCTCTTCCTGAAGATGCGGACGACCATGATGCGCTTGTTGTTCTGGGCGGCGAACAGAATGCGGTAGATGATG
TGCTTTATCCCTATCTTCCGGACTTGGCGCTTCTGATGAAGGCTTTCGGTGATGCGGGCAAGGCGGTCATGGGTGTTTGC
CTCGGCAGCCAGTTGCTGGCGCGCGCCTATGGCGGAGAGAATATTCTCTCAGGCCCGCCGGAATTCGGTTGGCAGGATGT
GTCCCTGACGCCGGAGGGCTTATCCGATCCGCTTTTGGCCGGGCAGCAGCAGAACTTCCCGATCTTTCAGTGGCATTGTG
ACACTTTTACGCTTCCGCCCGATGCCACGCGCCTTGCGACCAATGCCGCGACACAAAACCAGGCCTTTCGTATCGGACGG
GCGGCCTATGGCACCCAGTTCCATTTCGAGGCTTCCACCGAGGTTGTCGACGAATGGTGTCGGAGATTTCCGGGTTCCGT
CGAAAGAATGTCGCCCGGCTGGCTGGAAAATTACGGTGACCACCGTGGCACGCGGGCGCAGATGGCCGACATGACGGGTC
TGGAAATAGCCCGCGCCTGGATTCGGCTGATCTGA

Upstream 100 bases:

>100_bases
GTTTTTTCCCGTGGTGAAGCTGGACTCAATTGGCAATTTTTGCCGATTGAGTCCATCGTCGTCGCCAAGAGGTCTTATGC
GCGTTGCAATCATAGAAAAC

Downstream 100 bases:

>100_bases
CAGCGGTGATTTTCGGTTGCGAATTGAACAGGTTCTGCTCAAGATTGCGACGGGTTCACTTCGATTTGCAACAGAGGCGC
AAAGATCATGTATAAATTCG

Product: GMP synthase

Products: AMP; L-Glutamate; GMP; H+; Diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MAGTPHGQIGVALREAAADIHIIRAYAGDPLPEDADDHDALVVLGGEQNAVDDVLYPYLPDLALLMKAFGDAGKAVMGVC
LGSQLLARAYGGENILSGPPEFGWQDVSLTPEGLSDPLLAGQQQNFPIFQWHCDTFTLPPDATRLATNAATQNQAFRIGR
AAYGTQFHFEASTEVVDEWCRRFPGSVERMSPGWLENYGDHRGTRAQMADMTGLEIARAWIRLI

Sequences:

>Translated_224_residues
MAGTPHGQIGVALREAAADIHIIRAYAGDPLPEDADDHDALVVLGGEQNAVDDVLYPYLPDLALLMKAFGDAGKAVMGVC
LGSQLLARAYGGENILSGPPEFGWQDVSLTPEGLSDPLLAGQQQNFPIFQWHCDTFTLPPDATRLATNAATQNQAFRIGR
AAYGTQFHFEASTEVVDEWCRRFPGSVERMSPGWLENYGDHRGTRAQMADMTGLEIARAWIRLI
>Mature_223_residues
AGTPHGQIGVALREAAADIHIIRAYAGDPLPEDADDHDALVVLGGEQNAVDDVLYPYLPDLALLMKAFGDAGKAVMGVCL
GSQLLARAYGGENILSGPPEFGWQDVSLTPEGLSDPLLAGQQQNFPIFQWHCDTFTLPPDATRLATNAATQNQAFRIGRA
AYGTQFHFEASTEVVDEWCRRFPGSVERMSPGWLENYGDHRGTRAQMADMTGLEIARAWIRLI

Specific function: Catalyzes The Synthesis Of Gmp From Xmp. [C]

COG id: COG0518

COG function: function code F; GMP synthase - Glutamine amidotransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 6.3.5.2 [C]

Molecular weight: Translated: 24354; Mature: 24222

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGTPHGQIGVALREAAADIHIIRAYAGDPLPEDADDHDALVVLGGEQNAVDDVLYPYLP
CCCCCCCHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHH
DLALLMKAFGDAGKAVMGVCLGSQLLARAYGGENILSGPPEFGWQDVSLTPEGLSDPLLA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHC
GQQQNFPIFQWHCDTFTLPPDATRLATNAATQNQAFRIGRAAYGTQFHFEASTEVVDEWC
CCCCCCCEEEEECCEEECCCCHHHHHHHHHHCCHHHHHHHHHCCCEEEECCCHHHHHHHH
RRFPGSVERMSPGWLENYGDHRGTRAQMADMTGLEIARAWIRLI
HHCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AGTPHGQIGVALREAAADIHIIRAYAGDPLPEDADDHDALVVLGGEQNAVDDVLYPYLP
CCCCCCHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHH
DLALLMKAFGDAGKAVMGVCLGSQLLARAYGGENILSGPPEFGWQDVSLTPEGLSDPLLA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHC
GQQQNFPIFQWHCDTFTLPPDATRLATNAATQNQAFRIGRAAYGTQFHFEASTEVVDEWC
CCCCCCCEEEEECCEEECCCCHHHHHHHHHHCCHHHHHHHHHCCCEEEECCCHHHHHHHH
RRFPGSVERMSPGWLENYGDHRGTRAQMADMTGLEIARAWIRLI
HHCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 2 {1-ribosyl-4,6-dihydroxypyrazolo[3,4-d]-pyrimidine} 1 {NH4+}} 0.058 {XMP}} 0.029 {XMP}} 0.42 {6-thioXMP}} 0.53 {ATP}} 0.35 {8-azaXMP}} [C]

Substrates: ATP; L-Glutamine; H2O; Xanthosine 5'-phosphate [C]

Specific reaction: ATP + L-Glutamine + H2O + Xanthosine 5'-phosphate --> AMP + L-Glutamate + GMP + (2) H+ + Diphosphate [C]

General reaction: Amination [C]

Inhibitor: 1, N6-Ethenoadenosine; 1-Methyl adenosine 5'-phosphate 5'-phosphate; 2', 3'-Dihydroadenosine; 2'-Deoxyadenosine; 2, 6-Diaminopurine ribonucleoside; 2-Amino-6-methyl thiopurine ribonucleoside; 2-Fluoroadenosine; 3'-Deoxyadenosine; 5'-Azido-5'-deoxyadenosin

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7612925; 11677609 [H]