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The map label for this gene is proB

Identifier: 15890029

GI number: 15890029

Start: 2784811

End: 2785980

Strand: Reverse

Name: proB

Synonym: Atu2780

Alternate gene names: 15890029

Gene position: 2785980-2784811 (Counterclockwise)

Preceding gene: 159185379

Following gene: 159185378

Centisome position: 98.04

GC content: 60.94

Gene sequence:

>1170_bases
ATGAGCCTGACGCGCAAGCCGCTGGCGAGCCACCACCGGATCGTCATCAAGATCGGTTCGGCGCTTCTGGTGGACCGGAA
AAGCGGCCTGAAGAAAGACTGGCTGGATGCCATCTGCGAGGATATCGCGGCGCTGAAAAAAAACGGCGCGGATGTCCAGG
TCGTGTCTTCTGGCGCCATTGCGCTTGGCCGCACCGTGCTCGGCCTGCCTTCCGGTGCACTGAAGCTGGAAGAAAGCCAG
GCGGCGGCGGCAGTCGGCCAGATTGCGCTGGCGCGGGCATGGTCGGAAAGCCTGTCGCGGCATGAGATCGTCGCCGGTCA
AATTCTATTGACGCTATCCGATACCGAGGAGCGTCGGCGTTATCTCAACGCGCGTGCCACCATCAATCAGCTTTTGAAGA
TCGGCGCCATTCCGATCATCAATGAAAACGACACGGTGGCGACCACTGAAATCCGTTATGGGGACAATGACCGTCTGGCC
GCGCGCGTGGCCACGATGACGGGTGCCGATCTCCTGGTGCTGTTGTCGGATATTGACGGGCTCTACACCGCCCCACCGCA
TCTCGATCCCGATGCGAAATTTCTTGAGACGATTGCCGATATCACGCCCGAAATCGAAGCCATGGCGGGTGGGGCGGCCT
CCGAGCTTTCGCGCGGCGGTATGCGCACCAAGATCGATGCGGGCAAGATCGCGACCGCTGCCGGTTGTGGTATGATCATC
GCTTCCGGCAAGACGCTCAATCCGCTGAAGGCAATCGAAAACGGCGCACGGTCTTCATGGTTTGCACCCTCCGGCACGCC
GGTGACGGCCCGCAAGACCTGGATTGCCGGGCAATTGCAGCCGGCCGGCGAAATCCATGTGGATGCCGGGGCCGAAAAGG
CGCTTTATGCTGGGAAAAGCCTGCTTCCCGCCGGTGTGCGGCAGGTGAAGGGCAATTTCGGACGCGGCGATGCCATTGCC
ATCATCGGCGTCGAAGGCCGTGAAGTGGCGCGCGGTCTTGCGGGTTACGATGCAGAGGAAGCGCGCCTGATCATCGGCCA
CAAGTCCAACGAGATCGAGACGATCCTCGGTTATGTCGGACGGGCGGCGATGATCCATCGCGACGACCTTGTGATGACTG
GCGCTGCCGTCAAAGTGAAACATGCAAAGAAGGATGAGGTCCATGCCTGA

Upstream 100 bases:

>100_bases
TTCGCGACATCATCGTTTCGGCAAGCAATGGCGGCGACACCGCCCTTCCAGACCGGTCGATGCCGCATGAGAGCGAGGTC
GAGGAAGAGGACGACCGCCT

Downstream 100 bases:

>100_bases
ACAGGCCGTGAAGCAGAGCCATGATATCGATGCGCTGATGATGACCATCGGCGCTCAGGCGAAAGCTGCTTCGCGACCGT
TGTCCATCGCCGGCACGGAC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MSLTRKPLASHHRIVIKIGSALLVDRKSGLKKDWLDAICEDIAALKKNGADVQVVSSGAIALGRTVLGLPSGALKLEESQ
AAAAVGQIALARAWSESLSRHEIVAGQILLTLSDTEERRRYLNARATINQLLKIGAIPIINENDTVATTEIRYGDNDRLA
ARVATMTGADLLVLLSDIDGLYTAPPHLDPDAKFLETIADITPEIEAMAGGAASELSRGGMRTKIDAGKIATAAGCGMII
ASGKTLNPLKAIENGARSSWFAPSGTPVTARKTWIAGQLQPAGEIHVDAGAEKALYAGKSLLPAGVRQVKGNFGRGDAIA
IIGVEGREVARGLAGYDAEEARLIIGHKSNEIETILGYVGRAAMIHRDDLVMTGAAVKVKHAKKDEVHA

Sequences:

>Translated_389_residues
MSLTRKPLASHHRIVIKIGSALLVDRKSGLKKDWLDAICEDIAALKKNGADVQVVSSGAIALGRTVLGLPSGALKLEESQ
AAAAVGQIALARAWSESLSRHEIVAGQILLTLSDTEERRRYLNARATINQLLKIGAIPIINENDTVATTEIRYGDNDRLA
ARVATMTGADLLVLLSDIDGLYTAPPHLDPDAKFLETIADITPEIEAMAGGAASELSRGGMRTKIDAGKIATAAGCGMII
ASGKTLNPLKAIENGARSSWFAPSGTPVTARKTWIAGQLQPAGEIHVDAGAEKALYAGKSLLPAGVRQVKGNFGRGDAIA
IIGVEGREVARGLAGYDAEEARLIIGHKSNEIETILGYVGRAAMIHRDDLVMTGAAVKVKHAKKDEVHA
>Mature_388_residues
SLTRKPLASHHRIVIKIGSALLVDRKSGLKKDWLDAICEDIAALKKNGADVQVVSSGAIALGRTVLGLPSGALKLEESQA
AAAVGQIALARAWSESLSRHEIVAGQILLTLSDTEERRRYLNARATINQLLKIGAIPIINENDTVATTEIRYGDNDRLAA
RVATMTGADLLVLLSDIDGLYTAPPHLDPDAKFLETIADITPEIEAMAGGAASELSRGGMRTKIDAGKIATAAGCGMIIA
SGKTLNPLKAIENGARSSWFAPSGTPVTARKTWIAGQLQPAGEIHVDAGAEKALYAGKSLLPAGVRQVKGNFGRGDAIAI
IGVEGREVARGLAGYDAEEARLIIGHKSNEIETILGYVGRAAMIHRDDLVMTGAAVKVKHAKKDEVHA

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI62912457, Length=268, Percent_Identity=32.089552238806, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI21361368, Length=270, Percent_Identity=31.8518518518519, Blast_Score=122, Evalue=8e-28,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=41.5300546448087, Blast_Score=263, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI17569967, Length=258, Percent_Identity=33.7209302325581, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17569969, Length=258, Percent_Identity=33.7209302325581, Blast_Score=127, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6321822, Length=415, Percent_Identity=32.289156626506, Blast_Score=183, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6320506, Length=416, Percent_Identity=33.6538461538462, Blast_Score=181, Evalue=1e-46,
Organism=Drosophila melanogaster, GI21357643, Length=295, Percent_Identity=30.5084745762712, Blast_Score=111, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_AGRT5 (Q8UBS0)

Other databases:

- EMBL:   AE007869
- PIR:   AC2918
- PIR:   F97692
- RefSeq:   NP_355710.1
- ProteinModelPortal:   Q8UBS0
- SMR:   Q8UBS0
- STRING:   Q8UBS0
- GeneID:   1134818
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu2780
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- PhylomeDB:   Q8UBS0
- ProtClustDB:   PRK05429
- BioCyc:   ATUM176299-1:ATU2780-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40956; Mature: 40824

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTRKPLASHHRIVIKIGSALLVDRKSGLKKDWLDAICEDIAALKKNGADVQVVSSGAI
CCCCCCCCCCCCEEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCH
ALGRTVLGLPSGALKLEESQAAAAVGQIALARAWSESLSRHEIVAGQILLTLSDTEERRR
HHCHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCHHHHH
YLNARATINQLLKIGAIPIINENDTVATTEIRYGDNDRLAARVATMTGADLLVLLSDIDG
HHHHHHHHHHHHHHCCCEEECCCCCEEEEEEEECCCCCCEEEEEHHCCCHHEEEEECCCC
LYTAPPHLDPDAKFLETIADITPEIEAMAGGAASELSRGGMRTKIDAGKIATAAGCGMII
CCCCCCCCCCCHHHHHHHHHCCCCHHHHCCCCHHHHHCCCCEEEECCCCEEHHCCCCEEE
ASGKTLNPLKAIENGARSSWFAPSGTPVTARKTWIAGQLQPAGEIHVDAGAEKALYAGKS
ECCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEECCCCCHHHHCCCC
LLPAGVRQVKGNFGRGDAIAIIGVEGREVARGLAGYDAEEARLIIGHKSNEIETILGYVG
CCHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHC
RAAMIHRDDLVMTGAAVKVKHAKKDEVHA
CHHEEECCCEEEECCEEEEECCCCCCCCC
>Mature Secondary Structure 
SLTRKPLASHHRIVIKIGSALLVDRKSGLKKDWLDAICEDIAALKKNGADVQVVSSGAI
CCCCCCCCCCCEEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCH
ALGRTVLGLPSGALKLEESQAAAAVGQIALARAWSESLSRHEIVAGQILLTLSDTEERRR
HHCHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCHHHHH
YLNARATINQLLKIGAIPIINENDTVATTEIRYGDNDRLAARVATMTGADLLVLLSDIDG
HHHHHHHHHHHHHHCCCEEECCCCCEEEEEEEECCCCCCEEEEEHHCCCHHEEEEECCCC
LYTAPPHLDPDAKFLETIADITPEIEAMAGGAASELSRGGMRTKIDAGKIATAAGCGMII
CCCCCCCCCCCHHHHHHHHHCCCCHHHHCCCCHHHHHCCCCEEEECCCCEEHHCCCCEEE
ASGKTLNPLKAIENGARSSWFAPSGTPVTARKTWIAGQLQPAGEIHVDAGAEKALYAGKS
ECCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCCCCEEEECCCCCHHHHCCCC
LLPAGVRQVKGNFGRGDAIAIIGVEGREVARGLAGYDAEEARLIIGHKSNEIETILGYVG
CCHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHC
RAAMIHRDDLVMTGAAVKVKHAKKDEVHA
CHHEEECCCEEEECCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194