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The map label for this gene is purU [H]

Identifier: 15889735

GI number: 15889735

Start: 2433262

End: 2434146

Strand: Direct

Name: purU [H]

Synonym: Atu2464

Alternate gene names: 15889735

Gene position: 2433262-2434146 (Clockwise)

Preceding gene: 15889734

Following gene: 159185228

Centisome position: 85.63

GC content: 57.63

Gene sequence:

>885_bases
ATGACATCCTATGTTCTGACCGTAGCCTGCAAATCGACCCGCGGTATCGTCGCCGCGATTTCCGGTTATCTGGCGGAAAA
AGGCTGCAACATCGTCGACAGTTCGCAGTTTGACGACCTCGAGACCGGCAAATTCTTCATGCGCGTCTCCTTTATTTCGG
AAGAAGGTGCCTCCCTTCAGGCCATCACCGAAGGCTTTCAGCCGGTATCGGAAAAATTCGGCATGGCATCGGATATCTAC
CCGGACGGCCAGCGCATGAAGGCCATGCTGATGGTGTCGCGTTTCGGCCATTGCCTCAATGACCTGCTCTACCGCTGGAA
GATCGGCGCGTTGCCGATCGATATCGTCGGCGTCGTTTCCAACCATTTCGATTACCAGAAAGTGGTGGTCAATCACGACA
TCCCCTTCCACCACATCAAGGTGACGAAGGAGAACAAGCCGAAGGCCGAAGCTCAGCTGATGGACCTGATTGAGACCAGC
GGCACGGAGCTTGTCGTTCTGGCGCGCTATATGCAGGTGCTTTCCGACGAGATGTGCCGCAAGATGTCTGGCAAGATCAT
CAATATCCACCACTCCTTCCTGCCGAGCTTCAAGGGCGCAAACCCCTACAAGCAGGCCTATGAGCGTGGCGTGAAGCTGA
TCGGTGCAACGGCGCATTACGTCACCGGCGATCTCGACGAAGGCCCGATCATCGAACAGGACACGGTGCGTGTGACCCAC
GCACAATCGGCAGAAGACTATGTCTCGCTCGGCCGCGACGTGGAAAGCCAGGTGCTGGCCCGCGCCATCCACGCCCATAT
CCACCATCGCACCTTCATCAACGGTAACCGCACCGTGGTCTTCCCGCCGAGCCCCGGCAGCTATGCTTCCGAGCGGATGG
GGTGA

Upstream 100 bases:

>100_bases
GCCTTGGCGCTTTGTTCTTTGCTCCGTCACCAAGCTGCGCTATGCAACACGTCGAACTGCGCCGGCTTGATGCCGTCGCG
ACGTCTTTGGGGGAAAACGC

Downstream 100 bases:

>100_bases
TAAGGTGGCAGCAGACAAGGCTGCCTGATAGGCCGCCCTGCTATGCCGCTCTTTCCGGCGCGGCGAACACCACCCGCGCC
TTCAATCCGCGCCCGTCCGC

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTSYVLTVACKSTRGIVAAISGYLAEKGCNIVDSSQFDDLETGKFFMRVSFISEEGASLQAITEGFQPVSEKFGMASDIY
PDGQRMKAMLMVSRFGHCLNDLLYRWKIGALPIDIVGVVSNHFDYQKVVVNHDIPFHHIKVTKENKPKAEAQLMDLIETS
GTELVVLARYMQVLSDEMCRKMSGKIINIHHSFLPSFKGANPYKQAYERGVKLIGATAHYVTGDLDEGPIIEQDTVRVTH
AQSAEDYVSLGRDVESQVLARAIHAHIHHRTFINGNRTVVFPPSPGSYASERMG

Sequences:

>Translated_294_residues
MTSYVLTVACKSTRGIVAAISGYLAEKGCNIVDSSQFDDLETGKFFMRVSFISEEGASLQAITEGFQPVSEKFGMASDIY
PDGQRMKAMLMVSRFGHCLNDLLYRWKIGALPIDIVGVVSNHFDYQKVVVNHDIPFHHIKVTKENKPKAEAQLMDLIETS
GTELVVLARYMQVLSDEMCRKMSGKIINIHHSFLPSFKGANPYKQAYERGVKLIGATAHYVTGDLDEGPIIEQDTVRVTH
AQSAEDYVSLGRDVESQVLARAIHAHIHHRTFINGNRTVVFPPSPGSYASERMG
>Mature_293_residues
TSYVLTVACKSTRGIVAAISGYLAEKGCNIVDSSQFDDLETGKFFMRVSFISEEGASLQAITEGFQPVSEKFGMASDIYP
DGQRMKAMLMVSRFGHCLNDLLYRWKIGALPIDIVGVVSNHFDYQKVVVNHDIPFHHIKVTKENKPKAEAQLMDLIETSG
TELVVLARYMQVLSDEMCRKMSGKIINIHHSFLPSFKGANPYKQAYERGVKLIGATAHYVTGDLDEGPIIEQDTVRVTHA
QSAEDYVSLGRDVESQVLARAIHAHIHHRTFINGNRTVVFPPSPGSYASERMG

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=115, Percent_Identity=36.5217391304348, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI209869995, Length=115, Percent_Identity=36.5217391304348, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI209869993, Length=115, Percent_Identity=36.5217391304348, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1787483, Length=281, Percent_Identity=41.2811387900356, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI1788846, Length=168, Percent_Identity=27.9761904761905, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17567511, Length=164, Percent_Identity=32.9268292682927, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24582400, Length=89, Percent_Identity=38.2022471910112, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32671; Mature: 32540

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSYVLTVACKSTRGIVAAISGYLAEKGCNIVDSSQFDDLETGKFFMRVSFISEEGASLQ
CCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHH
AITEGFQPVSEKFGMASDIYPDGQRMKAMLMVSRFGHCLNDLLYRWKIGALPIDIVGVVS
HHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
NHFDYQKVVVNHDIPFHHIKVTKENKPKAEAQLMDLIETSGTELVVLARYMQVLSDEMCR
CCCCCEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KMSGKIINIHHSFLPSFKGANPYKQAYERGVKLIGATAHYVTGDLDEGPIIEQDTVRVTH
HHCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEECCHHEEECCCCCCCCCCCCCEEEEE
AQSAEDYVSLGRDVESQVLARAIHAHIHHRTFINGNRTVVFPPSPGSYASERMG
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHEEECCCEEEEECCCCCCCHHHCCC
>Mature Secondary Structure 
TSYVLTVACKSTRGIVAAISGYLAEKGCNIVDSSQFDDLETGKFFMRVSFISEEGASLQ
CCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHH
AITEGFQPVSEKFGMASDIYPDGQRMKAMLMVSRFGHCLNDLLYRWKIGALPIDIVGVVS
HHHHCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
NHFDYQKVVVNHDIPFHHIKVTKENKPKAEAQLMDLIETSGTELVVLARYMQVLSDEMCR
CCCCCEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KMSGKIINIHHSFLPSFKGANPYKQAYERGVKLIGATAHYVTGDLDEGPIIEQDTVRVTH
HHCCCEEEEEHHHCCCCCCCCHHHHHHHCCCEEEECCHHEEECCCCCCCCCCCCCEEEEE
AQSAEDYVSLGRDVESQVLARAIHAHIHHRTFINGNRTVVFPPSPGSYASERMG
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHEEECCCEEEEECCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]