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The map label for this gene is lpdA [H]

Identifier: 15888759

GI number: 15888759

Start: 1428507

End: 1429952

Strand: Direct

Name: lpdA [H]

Synonym: Atu1434

Alternate gene names: 15888759

Gene position: 1428507-1429952 (Clockwise)

Preceding gene: 15888758

Following gene: 159184759

Centisome position: 50.27

GC content: 59.41

Gene sequence:

>1446_bases
ATGGCTCAATCTTATGACGTCATCATCATCGGTTCGGGACCGGGCGGTTATATTGCCGCGATCCGCGCAGCCCAGCTCGG
CATGAAGGTTGCCGTCGTCGAGCGCGAACATCTGGCGGGCATCTGCTCCAACTGGGGCTGCATTCCTACCAAGGCCCTGC
TGCGCACCGCCGATGTCATGCACACCGCCACCCACGCCAAGGATTACGGCCTGACGCTGGAAGGTTCGATCAAGCCTGAC
GTGAAGGCAATCGTGGCGCGCTCGCGCGGCATTGCTGCGCGCATGAACAACGGCGTCGGTTTCCTGTTCAAGAAGAACAA
GGTCGATATCATCTGGGGTGAAGCGAAAATCACCAAGCCCGGCGAAATCGTTGTCGGCAAGTCCACCAAGCCGGTGGTTC
AGCCGCAGGGTCCGGTTCCGAAGAACACGCTGGGCGAGGGCACTTACACCGCCAAGCACATCATCGTCGCAACAGGCGCC
CGCCCGCGCGCGCTTCCGGGCATCGAGCCGGACGGCAAGCTGATCTGGACCTATTTCGAGGCGATGAAGCCGGAAGAGCT
GCCGAAGTCGCTGCTCGTCATGGGTTCGGGCGCCATCGGCATCGAATTCGCCAGCTTCTACCGCACCATGGGCGTCGATG
TTACCGTCGTCGAGATCATGAGCCAGGTCATGCCGGTCGAAGATGCGGAAATCTCTGCCTTTGCCAAGAAGCAGCTGGAA
AAGCAGGGTATCAAGATTCATCTGGAGACCAAGGTTTCCAAGGTCGAGAAGGCCGCGAATTCCGTTACCGCGACGTTGGA
GAAGAAGGACGGTTCGTCCGAGAAGATCATCGCTGACCGGATGATTTCGGCTGTCGGCGTCGTGGCGAATGTCGAAGGCA
TCGGCCTTGAGGCCGCCGGCGTGAAGACCGATCGCGGTTTCATCATCATTGACGGTTACGGCAAAACCAACGTGCCTGGT
ATTTACGCCATCGGCGATGTCGCCGGCCCGCCGCTGCTCGCCCATAAGGCCGAGCACGAGGCCGTCATCTGCGTTGAAAA
GATCGCTGGTCTGCCGAATGTCCATCCGATGGACAAGCTCAAGATCCCCGGCTGCACCTATTGCAACCCGCAGGTCGCCT
CGGTTGGTTTGACCGAAGCCAAGGCCAAGGAACAGGGCCGCGACATCCGCGTCGGCCGTTTCTCCTTTGCGGCGAACGGC
AAGGCGGTTGCGCTCGGTGAAGACCAGGGCATGGTCAAGACCATCTTCGACAAGAAGACCGGCGAACTGCTCGGTGCCCA
TATGGTCGGTGCGGAAGTGACCGAACTCATTCAGGGCTTCGTCGTTGCCATGAACCTCGAGACGACGGAAGAAGATTTGA
TGCACACGATCTTCCCGCATCCGACCATTTCGGAATCAATGAAGGAAAGCGTGCTCGACGCTTACGGACGTGTGCTGAAC
GCATAA

Upstream 100 bases:

>100_bases
CGGGCTGGAGCCCGTCGTCCGCATGATGCTCGGACTTTGACACTCATTCTCGTTTCGGGACGGTTTACCATCATCCGAAC
AAGACAAGGCAGGATTTCCA

Downstream 100 bases:

>100_bases
GTGTCCCGTGATGCCCGGCATTGTCTAACCTTGCCGGGTGTCCCATATGTCTCAACGCCGGTTCCTCAAGGGAACTGTTC
TTTCAGGGAGCGTGGGAATG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MAQSYDVIIIGSGPGGYIAAIRAAQLGMKVAVVEREHLAGICSNWGCIPTKALLRTADVMHTATHAKDYGLTLEGSIKPD
VKAIVARSRGIAARMNNGVGFLFKKNKVDIIWGEAKITKPGEIVVGKSTKPVVQPQGPVPKNTLGEGTYTAKHIIVATGA
RPRALPGIEPDGKLIWTYFEAMKPEELPKSLLVMGSGAIGIEFASFYRTMGVDVTVVEIMSQVMPVEDAEISAFAKKQLE
KQGIKIHLETKVSKVEKAANSVTATLEKKDGSSEKIIADRMISAVGVVANVEGIGLEAAGVKTDRGFIIIDGYGKTNVPG
IYAIGDVAGPPLLAHKAEHEAVICVEKIAGLPNVHPMDKLKIPGCTYCNPQVASVGLTEAKAKEQGRDIRVGRFSFAANG
KAVALGEDQGMVKTIFDKKTGELLGAHMVGAEVTELIQGFVVAMNLETTEEDLMHTIFPHPTISESMKESVLDAYGRVLN
A

Sequences:

>Translated_481_residues
MAQSYDVIIIGSGPGGYIAAIRAAQLGMKVAVVEREHLAGICSNWGCIPTKALLRTADVMHTATHAKDYGLTLEGSIKPD
VKAIVARSRGIAARMNNGVGFLFKKNKVDIIWGEAKITKPGEIVVGKSTKPVVQPQGPVPKNTLGEGTYTAKHIIVATGA
RPRALPGIEPDGKLIWTYFEAMKPEELPKSLLVMGSGAIGIEFASFYRTMGVDVTVVEIMSQVMPVEDAEISAFAKKQLE
KQGIKIHLETKVSKVEKAANSVTATLEKKDGSSEKIIADRMISAVGVVANVEGIGLEAAGVKTDRGFIIIDGYGKTNVPG
IYAIGDVAGPPLLAHKAEHEAVICVEKIAGLPNVHPMDKLKIPGCTYCNPQVASVGLTEAKAKEQGRDIRVGRFSFAANG
KAVALGEDQGMVKTIFDKKTGELLGAHMVGAEVTELIQGFVVAMNLETTEEDLMHTIFPHPTISESMKESVLDAYGRVLN
A
>Mature_480_residues
AQSYDVIIIGSGPGGYIAAIRAAQLGMKVAVVEREHLAGICSNWGCIPTKALLRTADVMHTATHAKDYGLTLEGSIKPDV
KAIVARSRGIAARMNNGVGFLFKKNKVDIIWGEAKITKPGEIVVGKSTKPVVQPQGPVPKNTLGEGTYTAKHIIVATGAR
PRALPGIEPDGKLIWTYFEAMKPEELPKSLLVMGSGAIGIEFASFYRTMGVDVTVVEIMSQVMPVEDAEISAFAKKQLEK
QGIKIHLETKVSKVEKAANSVTATLEKKDGSSEKIIADRMISAVGVVANVEGIGLEAAGVKTDRGFIIIDGYGKTNVPGI
YAIGDVAGPPLLAHKAEHEAVICVEKIAGLPNVHPMDKLKIPGCTYCNPQVASVGLTEAKAKEQGRDIRVGRFSFAANGK
AVALGEDQGMVKTIFDKKTGELLGAHMVGAEVTELIQGFVVAMNLETTEEDLMHTIFPHPTISESMKESVLDAYGRVLNA

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=484, Percent_Identity=36.5702479338843, Blast_Score=282, Evalue=4e-76,
Organism=Homo sapiens, GI50301238, Length=485, Percent_Identity=30.3092783505155, Blast_Score=178, Evalue=8e-45,
Organism=Homo sapiens, GI22035672, Length=471, Percent_Identity=27.8131634819533, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI291045266, Length=487, Percent_Identity=27.3100616016427, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI148277065, Length=486, Percent_Identity=27.7777777777778, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI33519430, Length=486, Percent_Identity=27.7777777777778, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI33519428, Length=486, Percent_Identity=27.7777777777778, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI33519426, Length=486, Percent_Identity=27.7777777777778, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI148277071, Length=486, Percent_Identity=27.7777777777778, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI291045268, Length=483, Percent_Identity=26.5010351966874, Blast_Score=102, Evalue=1e-21,
Organism=Escherichia coli, GI1786307, Length=464, Percent_Identity=36.8534482758621, Blast_Score=274, Evalue=1e-74,
Organism=Escherichia coli, GI87082354, Length=488, Percent_Identity=28.2786885245902, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1789915, Length=478, Percent_Identity=30.9623430962343, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI87081717, Length=478, Percent_Identity=28.0334728033473, Blast_Score=152, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI32565766, Length=487, Percent_Identity=37.1663244353183, Blast_Score=292, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI17557007, Length=489, Percent_Identity=27.19836400818, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71983429, Length=457, Percent_Identity=26.4770240700219, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI71983419, Length=457, Percent_Identity=26.4770240700219, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI71982272, Length=502, Percent_Identity=25.0996015936255, Blast_Score=99, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6321091, Length=486, Percent_Identity=37.4485596707819, Blast_Score=271, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6325166, Length=490, Percent_Identity=30, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6325240, Length=487, Percent_Identity=27.9260780287474, Blast_Score=169, Evalue=6e-43,
Organism=Drosophila melanogaster, GI21358499, Length=483, Percent_Identity=39.3374741200828, Blast_Score=307, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24640549, Length=488, Percent_Identity=27.2540983606557, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24640553, Length=488, Percent_Identity=27.2540983606557, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24640551, Length=488, Percent_Identity=27.2540983606557, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI17737741, Length=500, Percent_Identity=27.4, Blast_Score=125, Evalue=8e-29,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 51203; Mature: 51072

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQSYDVIIIGSGPGGYIAAIRAAQLGMKVAVVEREHLAGICSNWGCIPTKALLRTADVM
CCCCEEEEEEECCCCCHHHHHHHHHCCEEEEEEEHHHHHHHHCCCCCCCHHHHHHHHHHH
HTATHAKDYGLTLEGSIKPDVKAIVARSRGIAARMNNGVGFLFKKNKVDIIWGEAKITKP
HHHHCCHHCCEEEECCCCCCHHHHHHHCCCCEEEECCCEEEEEECCCEEEEECCEEECCC
GEIVVGKSTKPVVQPQGPVPKNTLGEGTYTAKHIIVATGARPRALPGIEPDGKLIWTYFE
CCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEHH
AMKPEELPKSLLVMGSGAIGIEFASFYRTMGVDVTVVEIMSQVMPVEDAEISAFAKKQLE
HCCHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
KQGIKIHLETKVSKVEKAANSVTATLEKKDGSSEKIIADRMISAVGVVANVEGIGLEAAG
HCCCEEEEEHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHEECCCCCEEECC
VKTDRGFIIIDGYGKTNVPGIYAIGDVAGPPLLAHKAEHEAVICVEKIAGLPNVHPMDKL
CCCCCCEEEEECCCCCCCCCEEEECCCCCCCCEEECCCCCEEEEEHHHCCCCCCCCCHHC
KIPGCTYCNPQVASVGLTEAKAKEQGRDIRVGRFSFAANGKAVALGEDQGMVKTIFDKKT
CCCCCCCCCCCHHCCCCHHHHHHHCCCCEEEEEEEEECCCCEEEEECCCCCHHHHHCCHH
GELLGAHMVGAEVTELIQGFVVAMNLETTEEDLMHTIFPHPTISESMKESVLDAYGRVLN
HHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
A
C
>Mature Secondary Structure 
AQSYDVIIIGSGPGGYIAAIRAAQLGMKVAVVEREHLAGICSNWGCIPTKALLRTADVM
CCCEEEEEEECCCCCHHHHHHHHHCCEEEEEEEHHHHHHHHCCCCCCCHHHHHHHHHHH
HTATHAKDYGLTLEGSIKPDVKAIVARSRGIAARMNNGVGFLFKKNKVDIIWGEAKITKP
HHHHCCHHCCEEEECCCCCCHHHHHHHCCCCEEEECCCEEEEEECCCEEEEECCEEECCC
GEIVVGKSTKPVVQPQGPVPKNTLGEGTYTAKHIIVATGARPRALPGIEPDGKLIWTYFE
CCEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEEHH
AMKPEELPKSLLVMGSGAIGIEFASFYRTMGVDVTVVEIMSQVMPVEDAEISAFAKKQLE
HCCHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
KQGIKIHLETKVSKVEKAANSVTATLEKKDGSSEKIIADRMISAVGVVANVEGIGLEAAG
HCCCEEEEEHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHEECCCCCEEECC
VKTDRGFIIIDGYGKTNVPGIYAIGDVAGPPLLAHKAEHEAVICVEKIAGLPNVHPMDKL
CCCCCCEEEEECCCCCCCCCEEEECCCCCCCCEEECCCCCEEEEEHHHCCCCCCCCCHHC
KIPGCTYCNPQVASVGLTEAKAKEQGRDIRVGRFSFAANGKAVALGEDQGMVKTIFDKKT
CCCCCCCCCCCHHCCCCHHHHHHHCCCCEEEEEEEEECCCCEEEEECCCCCHHHHHCCHH
GELLGAHMVGAEVTELIQGFVVAMNLETTEEDLMHTIFPHPTISESMKESVLDAYGRVLN
HHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]