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The map label for this gene is fixK [H]

Identifier: 15888379

GI number: 15888379

Start: 1029328

End: 1030080

Strand: Direct

Name: fixK [H]

Synonym: Atu1037

Alternate gene names: 15888379

Gene position: 1029328-1030080 (Clockwise)

Preceding gene: 159184589

Following gene: 15888380

Centisome position: 36.22

GC content: 56.97

Gene sequence:

>753_bases
ATGTCGCCAAAAAAACCGAACGGAATTTCCACTACCCCGTGCCAGCAATGTCCCTTGCGCGACCTGCCGCATTTCCGGGA
CTTTTCCTCCTCCGAACTCGATTTTGTTTCCCGTTTCAAGACGGGGGAACTCGCAGTGGAAAGCGGCTCTGTCATTCTGA
TGGAGGGCTCACACAGCGCTCATCTTTATACTGTCCTGCATGGCTGGGCTTTCCGATACAAGACGCTGGAGGACGGGCGC
CGACAGATCCTCAATTATGTGATGCCGGGCGACCTTGTTGGCCTTCAGGGCACGGTGATGGGGGAAATGGAGCACTCCGT
CGAAGCGCTGTCGCCGGTCACGCTCTGCGTCTTCGAGCGCTCCCGCCTCAATAATCTCTTCACTGATCATCCAACGCTCG
CATTTGACCTGACATGGATTGCGGCAAGCGAAGAACGCATGCTCGACAATCACCTCCTCAGCATCGGGCGTCGCACTGCC
ATCGAACGTGCCGCCTATCTCATTGCCTTCCTTTATCGCAAGGCCAAAGCAGCCAATTTGCTGCCTGGTGAAACACCGAT
CCCCGTCACCCAGCAGCATGTCGCCGACACACTCGGTCTTTCGATCGTCCACACCAATAAGACCCTGAGAAAGCTGGTCG
ATCGCGGCCTCATTCGCTGGATTGACAAGGGTTGCCTGGTGCTGGACGAGGCAGGCTTGAGCCATGTGGCGGGCTGGGAA
AGCGACGAAAAGCGGCAAAGGCCGTTCATCTGA

Upstream 100 bases:

>100_bases
ATGAGGTGGGAATATCTTCGTCTACCACCGGGAACCGAACACGTTCGGGCCGGTTGTTCCAAAACAATTGGGCGCAACCT
TTTTTTGATGATGGATATTC

Downstream 100 bases:

>100_bases
TTGTTTTACGACATGTCTTTTTTAAATGACGGTGAGGCCGCAATTTGCGACTGCTGTGATCATATCTCCCGCGAGAATTA
TACTGGAGACTGCTTTTTTT

Product: nitrogen fixation regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MSPKKPNGISTTPCQQCPLRDLPHFRDFSSSELDFVSRFKTGELAVESGSVILMEGSHSAHLYTVLHGWAFRYKTLEDGR
RQILNYVMPGDLVGLQGTVMGEMEHSVEALSPVTLCVFERSRLNNLFTDHPTLAFDLTWIAASEERMLDNHLLSIGRRTA
IERAAYLIAFLYRKAKAANLLPGETPIPVTQQHVADTLGLSIVHTNKTLRKLVDRGLIRWIDKGCLVLDEAGLSHVAGWE
SDEKRQRPFI

Sequences:

>Translated_250_residues
MSPKKPNGISTTPCQQCPLRDLPHFRDFSSSELDFVSRFKTGELAVESGSVILMEGSHSAHLYTVLHGWAFRYKTLEDGR
RQILNYVMPGDLVGLQGTVMGEMEHSVEALSPVTLCVFERSRLNNLFTDHPTLAFDLTWIAASEERMLDNHLLSIGRRTA
IERAAYLIAFLYRKAKAANLLPGETPIPVTQQHVADTLGLSIVHTNKTLRKLVDRGLIRWIDKGCLVLDEAGLSHVAGWE
SDEKRQRPFI
>Mature_249_residues
SPKKPNGISTTPCQQCPLRDLPHFRDFSSSELDFVSRFKTGELAVESGSVILMEGSHSAHLYTVLHGWAFRYKTLEDGRR
QILNYVMPGDLVGLQGTVMGEMEHSVEALSPVTLCVFERSRLNNLFTDHPTLAFDLTWIAASEERMLDNHLLSIGRRTAI
ERAAYLIAFLYRKAKAANLLPGETPIPVTQQHVADTLGLSIVHTNKTLRKLVDRGLIRWIDKGCLVLDEAGLSHVAGWES
DEKRQRPFI

Specific function: B.japonicum has two fixLJ-dependent fixK homologs that are activators of the transcription of a group of genes involved in anaerobic processes such as denitrification and possibly nitrogen fixation. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=225, Percent_Identity=22.6666666666667, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 28067; Mature: 27935

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPKKPNGISTTPCQQCPLRDLPHFRDFSSSELDFVSRFKTGELAVESGSVILMEGSHSA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEECCCEEEEECCCCC
HLYTVLHGWAFRYKTLEDGRRQILNYVMPGDLVGLQGTVMGEMEHSVEALSPVTLCVFER
EEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHCHHHHEEHHH
SRLNNLFTDHPTLAFDLTWIAASEERMLDNHLLSIGRRTAIERAAYLIAFLYRKAKAANL
HHHHHHCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LPGETPIPVTQQHVADTLGLSIVHTNKTLRKLVDRGLIRWIDKGCLVLDEAGLSHVAGWE
CCCCCCCCCCHHHHHHHHCEEEEECCHHHHHHHHHHHHHHHHCCCEEEECCCCHHHCCCC
SDEKRQRPFI
CCHHHCCCCC
>Mature Secondary Structure 
SPKKPNGISTTPCQQCPLRDLPHFRDFSSSELDFVSRFKTGELAVESGSVILMEGSHSA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEECCCEEEEECCCCC
HLYTVLHGWAFRYKTLEDGRRQILNYVMPGDLVGLQGTVMGEMEHSVEALSPVTLCVFER
EEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHCHHHHEEHHH
SRLNNLFTDHPTLAFDLTWIAASEERMLDNHLLSIGRRTAIERAAYLIAFLYRKAKAANL
HHHHHHCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
LPGETPIPVTQQHVADTLGLSIVHTNKTLRKLVDRGLIRWIDKGCLVLDEAGLSHVAGWE
CCCCCCCCCCHHHHHHHHCEEEEECCHHHHHHHHHHHHHHHHCCCEEEECCCCHHHCCCC
SDEKRQRPFI
CCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1551834; 12597275 [H]