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The map label for this gene is hpcH [H]

Identifier: 15888358

GI number: 15888358

Start: 1009441

End: 1010241

Strand: Direct

Name: hpcH [H]

Synonym: Atu1014

Alternate gene names: 15888358

Gene position: 1009441-1010241 (Clockwise)

Preceding gene: 15888357

Following gene: 159184578

Centisome position: 35.52

GC content: 62.55

Gene sequence:

>801_bases
ATGCCCGCTCCCGAAAACCGTTTCAAAACCGCCATCCATGCGGGCAAGCCGCAGATTGGCCTCTGGCTCGATATGGGCGA
GGCCATCACGGCGGAAATCGCCGGTACAGCGGGTTTCGACTGGCTGGTGATCGACGGGGAGCATGGGCCGAATGATCTGC
GCAGCATCATCGATCAGCTGCGGGCGCTTGCCACATCACCCGCCGAGCCTGTCGTGCGCGTGCCCGTTGGTGAAAGCTGG
ATGATCAAGCAGTTGCTGGATGCCGGCGCACGCACGCTCCTCGTGCCAATGGTTGATTCGGCGGAGCAGGCGAGTGATCT
GGTCAGTGCCATGCATTATCCGCCGCGTGGCATTCGCGGCATGGGCGCGGCGGTGGCCCGTGCCTCCGCCTTCAACACGA
TAGCCGATTATGCCGACAGCGCCTCGGATGGCGTCTGCCTGCTGGTGCAGGCCGAAACGCGGGCGGCGATCAACGATCTC
GACAATATTCTTGCGGTAGAAGGTGTGGACGGCGTCTTCATCGGCCCGGCCGATCTCGCCGCCGACATGGGTTATCTCGG
CCGGATCGACGAGCCGGAAGTGCAGGCCGTGATCGAAGCAGCGATCGTGAAGATCGTCGCGGCGGGCAAGGCTGCCGGCA
TTCTGACCTTCAACGAGGCCTATAATCGCCGTTATCTGGAGCTTGGCGCATCCTTCGTGGCTGTTGGTGCCGATGTGACG
GAATTCGCCAATACGCTTCGCGCGCTCTCCGCCCGTTACAAGGGCGGATCGGCACCTCAACCATCCAGATCGGGCTATTG
A

Upstream 100 bases:

>100_bases
GTCGCCGGCAAGAAACTGCTCGATATCGGTGCAGAGCCGGATCGCGCCATCCTGGCGGACGCTGCCGCCGATCTGAAATT
ATTGCTTTCCTGAGGATATC

Downstream 100 bases:

>100_bases
TCAGTCGTCGTTGCTGGCGTTCAGCTTTTCCGGCGGAATGCCTTCTTCGCCGGAAGCGAGATAGCCGCCGTTGATCAGAG
CGGCGCGAATGATGCGCTGC

Product: aldolase

Products: NA

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MPAPENRFKTAIHAGKPQIGLWLDMGEAITAEIAGTAGFDWLVIDGEHGPNDLRSIIDQLRALATSPAEPVVRVPVGESW
MIKQLLDAGARTLLVPMVDSAEQASDLVSAMHYPPRGIRGMGAAVARASAFNTIADYADSASDGVCLLVQAETRAAINDL
DNILAVEGVDGVFIGPADLAADMGYLGRIDEPEVQAVIEAAIVKIVAAGKAAGILTFNEAYNRRYLELGASFVAVGADVT
EFANTLRALSARYKGGSAPQPSRSGY

Sequences:

>Translated_266_residues
MPAPENRFKTAIHAGKPQIGLWLDMGEAITAEIAGTAGFDWLVIDGEHGPNDLRSIIDQLRALATSPAEPVVRVPVGESW
MIKQLLDAGARTLLVPMVDSAEQASDLVSAMHYPPRGIRGMGAAVARASAFNTIADYADSASDGVCLLVQAETRAAINDL
DNILAVEGVDGVFIGPADLAADMGYLGRIDEPEVQAVIEAAIVKIVAAGKAAGILTFNEAYNRRYLELGASFVAVGADVT
EFANTLRALSARYKGGSAPQPSRSGY
>Mature_265_residues
PAPENRFKTAIHAGKPQIGLWLDMGEAITAEIAGTAGFDWLVIDGEHGPNDLRSIIDQLRALATSPAEPVVRVPVGESWM
IKQLLDAGARTLLVPMVDSAEQASDLVSAMHYPPRGIRGMGAAVARASAFNTIADYADSASDGVCLLVQAETRAAINDLD
NILAVEGVDGVFIGPADLAADMGYLGRIDEPEVQAVIEAAIVKIVAAGKAAGILTFNEAYNRRYLELGASFVAVGADVTE
FANTLRALSARYKGGSAPQPSRSGY

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788578, Length=252, Percent_Identity=50.3968253968254, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1789514, Length=249, Percent_Identity=46.1847389558233, Blast_Score=229, Evalue=1e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.-

Molecular weight: Translated: 27935; Mature: 27804

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAPENRFKTAIHAGKPQIGLWLDMGEAITAEIAGTAGFDWLVIDGEHGPNDLRSIIDQL
CCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHH
RALATSPAEPVVRVPVGESWMIKQLLDAGARTLLVPMVDSAEQASDLVSAMHYPPRGIRG
HHHHCCCCCCEEECCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCH
MGAAVARASAFNTIADYADSASDGVCLLVQAETRAAINDLDNILAVEGVDGVFIGPADLA
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHEEECCCCEEEECHHHHH
ADMGYLGRIDEPEVQAVIEAAIVKIVAAGKAAGILTFNEAYNRRYLELGASFVAVGADVT
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCEEECCCHH
EFANTLRALSARYKGGSAPQPSRSGY
HHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
PAPENRFKTAIHAGKPQIGLWLDMGEAITAEIAGTAGFDWLVIDGEHGPNDLRSIIDQL
CCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHH
RALATSPAEPVVRVPVGESWMIKQLLDAGARTLLVPMVDSAEQASDLVSAMHYPPRGIRG
HHHHCCCCCCEEECCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCH
MGAAVARASAFNTIADYADSASDGVCLLVQAETRAAINDLDNILAVEGVDGVFIGPADLA
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHEEECCCCEEEECHHHHH
ADMGYLGRIDEPEVQAVIEAAIVKIVAAGKAAGILTFNEAYNRRYLELGASFVAVGADVT
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCEEECCCHH
EFANTLRALSARYKGGSAPQPSRSGY
HHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA