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The map label for this gene is thcD [H]

Identifier: 15888357

GI number: 15888357

Start: 1008216

End: 1009433

Strand: Direct

Name: thcD [H]

Synonym: Atu1013

Alternate gene names: 15888357

Gene position: 1008216-1009433 (Clockwise)

Preceding gene: 15888354

Following gene: 15888358

Centisome position: 35.48

GC content: 61.82

Gene sequence:

>1218_bases
GTGACGGGAAGGCTGGTAATCGTCGGGGCAGGGCAGGCCGCTTTCGCGCTGGCGTCGAAGCTTCGGGCGCTGAAGGACGA
GCGTCCCATCACCATCATCGGCTCCGAGGATGCCTATCCCTACCAGCGGCCGCCGCTCTCCAAAAAATACCTTCTCGGCG
AGATGAGCTTTGACCGGCTGATGTTCCGCCCCGAGGAATGGTACGCGGAAAACAACGTCGATATCCGGCTCTCTACCTGG
GTGGAGGAGATTGACCGCGCGGCGAAAACCGTGCGCATGCAGGACGGCAGCACACTTTCCTATGACAAGCTGGCGCTGGC
GACGGGTGCGGCACCCCGTCTGCTGCCCGCCAGCATTGGCGGCGATCTCGAAGGCGTATTGACGGTTCGCGACAAGCGCG
ATGCCGACCGGCTGGTGGAGGAGATGAAGCCGGGGCGCAGGCTGCTGGTGATCGGCGGCGGTTATATCGGCCTCGAAGCG
GCGGCGGTTGCCCGCAAGCTCGGCCTTGAAGTGACGCTGATCGAGATGGCCGACCGTATTCTCCAGCGCGTGGCAGCGAA
GGAAACCGCCGATATCATGCGCGGCATCCATCAGGCCCATGGCGTCTCTATCCGCGAAAAAACCGGGCTCGTGCGCCTTG
TCGGCATGGATGGCCGGGTGGCCGCTGCCGAACTTTCCGACGGTTCGATGCTCGATGTCGATTTTGTCATCGTCGGCATC
GGCGTGACTCCGAACGACCGGCTTGCCCGCGAATCGGGCCTTGATGTCGGCAACGGCATTGTCGTTGACGCTCTTACCCG
CACATCGGATGCAGATATTCATGCGGTCGGCGATTGCAACATGTTGCCGTGGCGTGGTCAGCATGTTCGTCTCGAATCGG
TACAGAACGCCGTTGATCAGGCTGAGGCCGCCGCTGAGGTGCTGGCCGGCACCGAAGCCGCCTATGAGGCGAAACCCTGG
TTCTGGTCGGATCAATATGAGGTGAAGCTGCAGATTGCCGGCTTCAATCTCGGTTACGACGAAACGATGCTGAGGCCCGG
TGCGCGCGAAGGCAGCTGGTCTGTCTGGTATTTCCGCGACGGTCGATTCGTGGCCGTCGATGCCGTCAATGATGCCAAGG
CCTATGTCGCCGGCAAGAAACTGCTCGATATCGGTGCAGAGCCGGATCGCGCCATCCTGGCGGACGCTGCCGCCGATCTG
AAATTATTGCTTTCCTGA

Upstream 100 bases:

>100_bases
CGGTTGACTGGGGCCGTACTTGGCGTTACGCCTGATCCTGCAATATGACTTCGCGAGGTGCGGCACTATCGGCGCTTCGT
GATTCTTCTGGGAGTGGACT

Downstream 100 bases:

>100_bases
GGATATCATGCCCGCTCCCGAAAACCGTTTCAAAACCGCCATCCATGCGGGCAAGCCGCAGATTGGCCTCTGGCTCGATA
TGGGCGAGGCCATCACGGCG

Product: ferredoxin reductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MTGRLVIVGAGQAAFALASKLRALKDERPITIIGSEDAYPYQRPPLSKKYLLGEMSFDRLMFRPEEWYAENNVDIRLSTW
VEEIDRAAKTVRMQDGSTLSYDKLALATGAAPRLLPASIGGDLEGVLTVRDKRDADRLVEEMKPGRRLLVIGGGYIGLEA
AAVARKLGLEVTLIEMADRILQRVAAKETADIMRGIHQAHGVSIREKTGLVRLVGMDGRVAAAELSDGSMLDVDFVIVGI
GVTPNDRLARESGLDVGNGIVVDALTRTSDADIHAVGDCNMLPWRGQHVRLESVQNAVDQAEAAAEVLAGTEAAYEAKPW
FWSDQYEVKLQIAGFNLGYDETMLRPGAREGSWSVWYFRDGRFVAVDAVNDAKAYVAGKKLLDIGAEPDRAILADAAADL
KLLLS

Sequences:

>Translated_405_residues
MTGRLVIVGAGQAAFALASKLRALKDERPITIIGSEDAYPYQRPPLSKKYLLGEMSFDRLMFRPEEWYAENNVDIRLSTW
VEEIDRAAKTVRMQDGSTLSYDKLALATGAAPRLLPASIGGDLEGVLTVRDKRDADRLVEEMKPGRRLLVIGGGYIGLEA
AAVARKLGLEVTLIEMADRILQRVAAKETADIMRGIHQAHGVSIREKTGLVRLVGMDGRVAAAELSDGSMLDVDFVIVGI
GVTPNDRLARESGLDVGNGIVVDALTRTSDADIHAVGDCNMLPWRGQHVRLESVQNAVDQAEAAAEVLAGTEAAYEAKPW
FWSDQYEVKLQIAGFNLGYDETMLRPGAREGSWSVWYFRDGRFVAVDAVNDAKAYVAGKKLLDIGAEPDRAILADAAADL
KLLLS
>Mature_404_residues
TGRLVIVGAGQAAFALASKLRALKDERPITIIGSEDAYPYQRPPLSKKYLLGEMSFDRLMFRPEEWYAENNVDIRLSTWV
EEIDRAAKTVRMQDGSTLSYDKLALATGAAPRLLPASIGGDLEGVLTVRDKRDADRLVEEMKPGRRLLVIGGGYIGLEAA
AVARKLGLEVTLIEMADRILQRVAAKETADIMRGIHQAHGVSIREKTGLVRLVGMDGRVAAAELSDGSMLDVDFVIVGIG
VTPNDRLARESGLDVGNGIVVDALTRTSDADIHAVGDCNMLPWRGQHVRLESVQNAVDQAEAAAEVLAGTEAAYEAKPWF
WSDQYEVKLQIAGFNLGYDETMLRPGAREGSWSVWYFRDGRFVAVDAVNDAKAYVAGKKLLDIGAEPDRAILADAAADLK
LLLS

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=371, Percent_Identity=27.7628032345013, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI226437568, Length=371, Percent_Identity=27.7628032345013, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI65787454, Length=371, Percent_Identity=27.7628032345013, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI4757732, Length=337, Percent_Identity=27.0029673590504, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI22202629, Length=337, Percent_Identity=27.0029673590504, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1788892, Length=403, Percent_Identity=35.2357320099256, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1789065, Length=279, Percent_Identity=32.6164874551971, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1789765, Length=354, Percent_Identity=28.8135593220339, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786307, Length=244, Percent_Identity=28.2786885245902, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=377, Percent_Identity=29.1777188328912, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24639257, Length=360, Percent_Identity=26.9444444444444, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI281359715, Length=360, Percent_Identity=26.9444444444444, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI281359713, Length=360, Percent_Identity=26.9444444444444, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24639250, Length=360, Percent_Identity=26.9444444444444, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI18543267, Length=360, Percent_Identity=26.9444444444444, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24639252, Length=360, Percent_Identity=26.9444444444444, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI28573993, Length=357, Percent_Identity=27.1708683473389, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24581020, Length=357, Percent_Identity=27.1708683473389, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585130, Length=363, Percent_Identity=23.9669421487603, Blast_Score=99, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 44066; Mature: 43935

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGRLVIVGAGQAAFALASKLRALKDERPITIIGSEDAYPYQRPPLSKKYLLGEMSFDRL
CCCCEEEEECCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHEECCCCHHHH
MFRPEEWYAENNVDIRLSTWVEEIDRAAKTVRMQDGSTLSYDKLALATGAAPRLLPASIG
EECCHHHCCCCCCEEEEHHHHHHHHHHHHEEEECCCCCCCCCCEEEECCCCCCEEEHHCC
GDLEGVLTVRDKRDADRLVEEMKPGRRLLVIGGGYIGLEAAAVARKLGLEVTLIEMADRI
CCCEEEEEEECCCCHHHHHHHCCCCCEEEEEECCEEHHHHHHHHHHHCCEEEHHHHHHHH
LQRVAAKETADIMRGIHQAHGVSIREKTGLVRLVGMDGRVAAAELSDGSMLDVDFVIVGI
HHHHHHHHHHHHHHHHHHHHCCEEECCCCEEEEEECCCCEEEEECCCCCEEEEEEEEEEE
GVTPNDRLARESGLDVGNGIVVDALTRTSDADIHAVGDCNMLPWRGQHVRLESVQNAVDQ
CCCCCHHHHHHCCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCCEEEHHHHHHHHHH
AEAAAEVLAGTEAAYEAKPWFWSDQYEVKLQIAGFNLGYDETMLRPGAREGSWSVWYFRD
HHHHHHHHHCCCCCCCCCCCEECCCEEEEEEEEEEECCCCHHHCCCCCCCCCEEEEEEEC
GRFVAVDAVNDAKAYVAGKKLLDIGAEPDRAILADAAADLKLLLS
CEEEEEEECCCCHHHHCCCHHEECCCCCCHHEEHHCCCCEEEECC
>Mature Secondary Structure 
TGRLVIVGAGQAAFALASKLRALKDERPITIIGSEDAYPYQRPPLSKKYLLGEMSFDRL
CCCEEEEECCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHEECCCCHHHH
MFRPEEWYAENNVDIRLSTWVEEIDRAAKTVRMQDGSTLSYDKLALATGAAPRLLPASIG
EECCHHHCCCCCCEEEEHHHHHHHHHHHHEEEECCCCCCCCCCEEEECCCCCCEEEHHCC
GDLEGVLTVRDKRDADRLVEEMKPGRRLLVIGGGYIGLEAAAVARKLGLEVTLIEMADRI
CCCEEEEEEECCCCHHHHHHHCCCCCEEEEEECCEEHHHHHHHHHHHCCEEEHHHHHHHH
LQRVAAKETADIMRGIHQAHGVSIREKTGLVRLVGMDGRVAAAELSDGSMLDVDFVIVGI
HHHHHHHHHHHHHHHHHHHHCCEEECCCCEEEEEECCCCEEEEECCCCCEEEEEEEEEEE
GVTPNDRLARESGLDVGNGIVVDALTRTSDADIHAVGDCNMLPWRGQHVRLESVQNAVDQ
CCCCCHHHHHHCCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCCEEEHHHHHHHHHH
AEAAAEVLAGTEAAYEAKPWFWSDQYEVKLQIAGFNLGYDETMLRPGAREGSWSVWYFRD
HHHHHHHHHCCCCCCCCCCCEECCCEEEEEEEEEEECCCCHHHCCCCCCCCCEEEEEEEC
GRFVAVDAVNDAKAYVAGKKLLDIGAEPDRAILADAAADLKLLLS
CEEEEEEECCCCHHHHCCCHHEECCCCCCHHEEHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]