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The map label for this gene is argF

Identifier: 15887776

GI number: 15887776

Start: 425285

End: 426202

Strand: Direct

Name: argF

Synonym: Atu0429

Alternate gene names: 15887776

Gene position: 425285-426202 (Clockwise)

Preceding gene: 15887775

Following gene: 159184304

Centisome position: 14.97

GC content: 61.0

Gene sequence:

>918_bases
ATGGCTTCACCTAAACATTTTCTCGACCTGTCGGCCGTTGGCCCGCAGGACCTGCGGACAATTCTTGACGATGCGCGCGC
GCGCAAGGTCGCCACGAAGGCGGGAACGGCGGAAAAGCCGCTGGCCGGCAAGATGCTGGCCATGATCTTCGAAAAGCCGT
CCACCCGCACCCGCGTTTCCTTCGATGTGGGCATGCGCCAGCTCGGCGGCGAGACCCTTTTCCTGTCGGGCACTGAAATG
CAGCTCGGTCGTGCGGAAACGATCGGCGATACCGCCAAGGTGCTGTCGCGTTATGTGGATGCCATCATGATCCGTACCAC
GGATCATTCGCGCCTGCTCGAGCTTGCCGAACACGCGACGGTGCCTGTTATCAACGGCCTGACCGATGATACCCATCCCT
GCCAGATCATGGCCGACATCATGACGTTCGAGGAACATCGTGGTCCGGTCAAAGGCAAGACCATTGCCTGGACGGGCGAC
GGCAACAACGTGCTGCACTCTTTCGTCGAGGGCTCCGCACGTTTCGGTTACCGCATGACGATGGCGGTGCCGATGGGTTC
CGAACCGCATGACAAGTTCATGAACTGGGCCCGCAACAATGGCGGCGAGATCGCGCTGTATCATGATGCGGACAAGGCGG
TCGCGGGTGCGGATTGTGTCGTCACCGATACCTGGGTGTCCATGAACCAGGAGCACAAGGCGCGCGGCCACAACATCTTC
CAGCCCTATCAGGTCAATGAGGCCCTGATGGCCAAGGCGCAGAAGGACGCGCTGTTCATGCACTGCCTGCCGGCGCATCG
CGGTGAAGAAGTGACGGACGCCGTGATCGACGGCCCGCAATCGGTGGTCTTCGATGAGGCGGAGAACCGTCTCCACGCGC
AGAAATCCGTCATCGCATGGTGCATGGGCGTGATCTGA

Upstream 100 bases:

>100_bases
CGGCAGCCGCATCCCTGACCGCCAAGCAGGCGAAAATCGCCTGAGGAAACAATAAAAGCGGGCCTCTTCCTGGAGGCTCG
CGAGCATGGGATAGTTTGAA

Downstream 100 bases:

>100_bases
GAAATTCACCCGTCCTGATCGTCTTCGGGCCGGGTGCTTGAACTGACACGCATCGATTACCATCTGTGGGGCTTCGAAGA
GACGCGTCGGTTTGCTGGCG

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MASPKHFLDLSAVGPQDLRTILDDARARKVATKAGTAEKPLAGKMLAMIFEKPSTRTRVSFDVGMRQLGGETLFLSGTEM
QLGRAETIGDTAKVLSRYVDAIMIRTTDHSRLLELAEHATVPVINGLTDDTHPCQIMADIMTFEEHRGPVKGKTIAWTGD
GNNVLHSFVEGSARFGYRMTMAVPMGSEPHDKFMNWARNNGGEIALYHDADKAVAGADCVVTDTWVSMNQEHKARGHNIF
QPYQVNEALMAKAQKDALFMHCLPAHRGEEVTDAVIDGPQSVVFDEAENRLHAQKSVIAWCMGVI

Sequences:

>Translated_305_residues
MASPKHFLDLSAVGPQDLRTILDDARARKVATKAGTAEKPLAGKMLAMIFEKPSTRTRVSFDVGMRQLGGETLFLSGTEM
QLGRAETIGDTAKVLSRYVDAIMIRTTDHSRLLELAEHATVPVINGLTDDTHPCQIMADIMTFEEHRGPVKGKTIAWTGD
GNNVLHSFVEGSARFGYRMTMAVPMGSEPHDKFMNWARNNGGEIALYHDADKAVAGADCVVTDTWVSMNQEHKARGHNIF
QPYQVNEALMAKAQKDALFMHCLPAHRGEEVTDAVIDGPQSVVFDEAENRLHAQKSVIAWCMGVI
>Mature_304_residues
ASPKHFLDLSAVGPQDLRTILDDARARKVATKAGTAEKPLAGKMLAMIFEKPSTRTRVSFDVGMRQLGGETLFLSGTEMQ
LGRAETIGDTAKVLSRYVDAIMIRTTDHSRLLELAEHATVPVINGLTDDTHPCQIMADIMTFEEHRGPVKGKTIAWTGDG
NNVLHSFVEGSARFGYRMTMAVPMGSEPHDKFMNWARNNGGEIALYHDADKAVAGADCVVTDTWVSMNQEHKARGHNIFQ
PYQVNEALMAKAQKDALFMHCLPAHRGEEVTDAVIDGPQSVVFDEAENRLHAQKSVIAWCMGVI

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=255, Percent_Identity=45.0980392156863, Blast_Score=222, Evalue=4e-58,
Organism=Homo sapiens, GI18105007, Length=307, Percent_Identity=24.4299674267101, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1790703, Length=325, Percent_Identity=38.4615384615385, Blast_Score=204, Evalue=6e-54,
Organism=Escherichia coli, GI1786469, Length=330, Percent_Identity=37.2727272727273, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI2367364, Length=313, Percent_Identity=27.1565495207668, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI48994908, Length=341, Percent_Identity=26.099706744868, Blast_Score=78, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI193204318, Length=284, Percent_Identity=25.3521126760563, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322373, Length=317, Percent_Identity=34.384858044164, Blast_Score=189, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6322331, Length=308, Percent_Identity=24.3506493506494, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24642586, Length=308, Percent_Identity=25, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_AGRT5 (Q8UI70)

Other databases:

- EMBL:   AE007869
- PIR:   A97411
- PIR:   AC2629
- RefSeq:   NP_353457.1
- ProteinModelPortal:   Q8UI70
- SMR:   Q8UI70
- STRING:   Q8UI70
- GeneID:   1132467
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0429
- eggNOG:   COG0078
- HOGENOM:   HBG579429
- OMA:   CVVTDTW
- PhylomeDB:   Q8UI70
- ProtClustDB:   PRK00779
- BioCyc:   ATUM176299-1:ATU0429-MONOMER
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 33518; Mature: 33386

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 105-105 BINDING 132-132

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASPKHFLDLSAVGPQDLRTILDDARARKVATKAGTAEKPLAGKMLAMIFEKPSTRTRVS
CCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEE
FDVGMRQLGGETLFLSGTEMQLGRAETIGDTAKVLSRYVDAIMIRTTDHSRLLELAEHAT
HHHHHHHHCCCEEEEECCCCCCCCCHHCCHHHHHHHHHHHEEEEEECCHHHHHHHHHHCC
VPVINGLTDDTHPCQIMADIMTFEEHRGPVKGKTIAWTGDGNNVLHSFVEGSARFGYRMT
CCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHCCCCCCCEEEE
MAVPMGSEPHDKFMNWARNNGGEIALYHDADKAVAGADCVVTDTWVSMNQEHKARGHNIF
EEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEEHHHHCCHHHHHCCCCCC
QPYQVNEALMAKAQKDALFMHCLPAHRGEEVTDAVIDGPQSVVFDEAENRLHAQKSVIAW
CCCCCCHHHHHHHHHCEEEEEECCCCCCCHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHH
CMGVI
HHHCC
>Mature Secondary Structure 
ASPKHFLDLSAVGPQDLRTILDDARARKVATKAGTAEKPLAGKMLAMIFEKPSTRTRVS
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEE
FDVGMRQLGGETLFLSGTEMQLGRAETIGDTAKVLSRYVDAIMIRTTDHSRLLELAEHAT
HHHHHHHHCCCEEEEECCCCCCCCCHHCCHHHHHHHHHHHEEEEEECCHHHHHHHHHHCC
VPVINGLTDDTHPCQIMADIMTFEEHRGPVKGKTIAWTGDGNNVLHSFVEGSARFGYRMT
CCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHCCCCCCCEEEE
MAVPMGSEPHDKFMNWARNNGGEIALYHDADKAVAGADCVVTDTWVSMNQEHKARGHNIF
EEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEEEHHHHCCHHHHHCCCCCC
QPYQVNEALMAKAQKDALFMHCLPAHRGEEVTDAVIDGPQSVVFDEAENRLHAQKSVIAW
CCCCCCHHHHHHHHHCEEEEEECCCCCCCHHHHHHHCCCHHEEHHHHHHHHHHHHHHHHH
CMGVI
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194