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The map label for this gene is fmt

Identifier: 15887718

GI number: 15887718

Start: 361403

End: 362338

Strand: Direct

Name: fmt

Synonym: Atu0367

Alternate gene names: 15887718

Gene position: 361403-362338 (Clockwise)

Preceding gene: 15887717

Following gene: 15887719

Centisome position: 12.72

GC content: 64.0

Gene sequence:

>936_bases
ATGTCTCTTCGCATCATTTTTATGGGCACGCCGGATTTCTCCGTTCCCACATTGCGTGCATTGGTGGAGGCGGGCCACGA
GATCGTTGCCGTCTACACCCAGCCGCCGCGTCCCGGCGGCCGCCGTGGTCTCGATTTGCAGAAGTCGCCGGTCCATCAGG
TGGCCGAATTGCTCGGGCTGCCTGTACTGACGCCCGTGAATTTCAAGGCGGAGGAAGATCGCCAGCAGTTCCGGGATTTC
AACGCCGATGTGGCGGTCGTCGTGGCTTACGGCCTGCTGCTGCCTGAAGCGATCCTGTCCGGCACGCGTCTTGGCTGTTA
CAATGGTCACGCCTCGCTGCTGCCGCGCTGGCGCGGGGCAGCCCCCATCCAGCGGGCGATCATGGCCGGCGATGCCGAAA
CCGGCATGATGGTCATGAAAATGGAAAAGGGGCTCGATACCGGCCCCGTCGCGCTTACCGCGAAGGTCGCGATCGACGAG
AATATGACGGCTGGCGAACTGCACGACAGCCTGATGCTGGCAGGTGCGCGGCTGATGCGGCAGGCGATGGCCAAGCTGGA
GGCCGACGACCTGCCCCTCGTCACGCAGGCGGAAGAGGGCGTGCTCTACGCCGCGAAGATCGACAAGGGTGAGACCCGTA
TCGATTTTTCAAGGCCCGCACAGGATGTCCATAACCACATCCGTGGTCTTTCGCCGTTTCCCGGCGCATGGTTGGAGATG
GATATCGGCGGCAAGCCAGAGCGCGTCAAGGTGCTGGCTTCGGAACTGGCCAGCGGCATGGGCGAAGCGGGCAGCGCTCT
CGATGACGCGCTCACCATTGCCTGCGGCAGCGATGCCGTGCGGCTCACACGCTTGCAGAAGGCGGGCGGCAAGCCCATGT
CGGCGGCCGATTTCGTGCGTGGTACGCCGGTTCCAGCCACAACGAGGCTCGGCTGA

Upstream 100 bases:

>100_bases
TGAAGCGCGACATGGTCATCAAGAAATTCACGAAAGCGGCCCGCGCAAAGATCTGATCCGTCCTTTTGGGCCGGAACGGA
CGACAGGGCAGGGGGCTACG

Downstream 100 bases:

>100_bases
TGCCACGTTTCCGCATGACCGTCGAATATGACGGCACGCCCTATTACGGCTGGCAGAGACAGGAAAACGGCCCCTCCGTT
CAGGGCGCGCTGGAGGCTGC

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MSLRIIFMGTPDFSVPTLRALVEAGHEIVAVYTQPPRPGGRRGLDLQKSPVHQVAELLGLPVLTPVNFKAEEDRQQFRDF
NADVAVVVAYGLLLPEAILSGTRLGCYNGHASLLPRWRGAAPIQRAIMAGDAETGMMVMKMEKGLDTGPVALTAKVAIDE
NMTAGELHDSLMLAGARLMRQAMAKLEADDLPLVTQAEEGVLYAAKIDKGETRIDFSRPAQDVHNHIRGLSPFPGAWLEM
DIGGKPERVKVLASELASGMGEAGSALDDALTIACGSDAVRLTRLQKAGGKPMSAADFVRGTPVPATTRLG

Sequences:

>Translated_311_residues
MSLRIIFMGTPDFSVPTLRALVEAGHEIVAVYTQPPRPGGRRGLDLQKSPVHQVAELLGLPVLTPVNFKAEEDRQQFRDF
NADVAVVVAYGLLLPEAILSGTRLGCYNGHASLLPRWRGAAPIQRAIMAGDAETGMMVMKMEKGLDTGPVALTAKVAIDE
NMTAGELHDSLMLAGARLMRQAMAKLEADDLPLVTQAEEGVLYAAKIDKGETRIDFSRPAQDVHNHIRGLSPFPGAWLEM
DIGGKPERVKVLASELASGMGEAGSALDDALTIACGSDAVRLTRLQKAGGKPMSAADFVRGTPVPATTRLG
>Mature_310_residues
SLRIIFMGTPDFSVPTLRALVEAGHEIVAVYTQPPRPGGRRGLDLQKSPVHQVAELLGLPVLTPVNFKAEEDRQQFRDFN
ADVAVVVAYGLLLPEAILSGTRLGCYNGHASLLPRWRGAAPIQRAIMAGDAETGMMVMKMEKGLDTGPVALTAKVAIDEN
MTAGELHDSLMLAGARLMRQAMAKLEADDLPLVTQAEEGVLYAAKIDKGETRIDFSRPAQDVHNHIRGLSPFPGAWLEMD
IGGKPERVKVLASELASGMGEAGSALDDALTIACGSDAVRLTRLQKAGGKPMSAADFVRGTPVPATTRLG

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI21614513, Length=319, Percent_Identity=28.2131661442006, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI238814322, Length=245, Percent_Identity=28.1632653061224, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI164663775, Length=322, Percent_Identity=29.8136645962733, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1789683, Length=302, Percent_Identity=44.3708609271523, Blast_Score=235, Evalue=3e-63,
Organism=Escherichia coli, GI1788589, Length=311, Percent_Identity=25.4019292604502, Blast_Score=106, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI133930964, Length=268, Percent_Identity=28.7313432835821, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319458, Length=193, Percent_Identity=32.1243523316062, Blast_Score=73, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45550868, Length=315, Percent_Identity=32.6984126984127, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI28571984, Length=229, Percent_Identity=35.3711790393013, Blast_Score=104, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24585660, Length=267, Percent_Identity=25.8426966292135, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_AGRT5 (Q8UID0)

Other databases:

- EMBL:   AE007869
- PIR:   AG2621
- PIR:   G97403
- RefSeq:   NP_353399.1
- ProteinModelPortal:   Q8UID0
- SMR:   Q8UID0
- STRING:   Q8UID0
- GeneID:   1132405
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0367
- eggNOG:   COG0223
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- PhylomeDB:   Q8UID0
- ProtClustDB:   PRK00005
- BioCyc:   ATUM176299-1:ATU0367-MONOMER
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 33170; Mature: 33039

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRIIFMGTPDFSVPTLRALVEAGHEIVAVYTQPPRPGGRRGLDLQKSPVHQVAELLGL
CEEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCC
PVLTPVNFKAEEDRQQFRDFNADVAVVVAYGLLLPEAILSGTRLGCYNGHASLLPRWRGA
CCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHCCCCCCC
APIQRAIMAGDAETGMMVMKMEKGLDTGPVALTAKVAIDENMTAGELHDSLMLAGARLMR
CHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHH
QAMAKLEADDLPLVTQAEEGVLYAAKIDKGETRIDFSRPAQDVHNHIRGLSPFPGAWLEM
HHHHHCCCCCCCEEEECCCCEEEEEEECCCCCEEECCCCHHHHHHHHCCCCCCCCCEEEE
DIGGKPERVKVLASELASGMGEAGSALDDALTIACGSDAVRLTRLQKAGGKPMSAADFVR
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHEEEEECCCHHHHHHHHHCCCCCCCHHHHHC
GTPVPATTRLG
CCCCCCCCCCC
>Mature Secondary Structure 
SLRIIFMGTPDFSVPTLRALVEAGHEIVAVYTQPPRPGGRRGLDLQKSPVHQVAELLGL
EEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCC
PVLTPVNFKAEEDRQQFRDFNADVAVVVAYGLLLPEAILSGTRLGCYNGHASLLPRWRGA
CCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCHHHCCCCCCC
APIQRAIMAGDAETGMMVMKMEKGLDTGPVALTAKVAIDENMTAGELHDSLMLAGARLMR
CHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHH
QAMAKLEADDLPLVTQAEEGVLYAAKIDKGETRIDFSRPAQDVHNHIRGLSPFPGAWLEM
HHHHHCCCCCCCEEEECCCCEEEEEEECCCCCEEECCCCHHHHHHHHCCCCCCCCCEEEE
DIGGKPERVKVLASELASGMGEAGSALDDALTIACGSDAVRLTRLQKAGGKPMSAADFVR
CCCCCCHHHHHHHHHHHHCCCCCCCHHHHHEEEEECCCHHHHHHHHHCCCCCCCHHHHHC
GTPVPATTRLG
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194