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The map label for this gene is def

Identifier: 15887717

GI number: 15887717

Start: 360846

End: 361358

Strand: Direct

Name: def

Synonym: Atu0366

Alternate gene names: 15887717

Gene position: 360846-361358 (Clockwise)

Preceding gene: 15887714

Following gene: 15887718

Centisome position: 12.7

GC content: 57.5

Gene sequence:

>513_bases
ATGACCATCAAACCGCTTATCATTTTGCCCGATCCCGTGCTGCGCCAGCAATCGAAGCTCATCGAACAGGTGGACGCCGA
AGTGCTGCGCCTTGCCGACGACATGCTCGAAACCATGTATGATGCGCCCGGTATCGGCCTCGCCGCTATTCAGATCGGCG
TGCCGCGCCGCATGCTGGTGATCGACGTGGCACGCGAAGGTGAAGAAAAGACCCCGGTCGTTTTCATCAATCCGGAAATC
CTCAAAGTGTCGGACGATATCTCGACCTATGAGGAAGGCTGCCTCTCCATTCCCGACTATTATGCCGAAGTGGAGCGTCC
CGCGTCGCTCACGGTGCAATATGTCGGGCGTGACGGCAAGCAGCAGACGGTCGAAGCGGATGGTCTGCTCGCGACCTGCC
TGCAGCATGAGATCGACCACCTCAACGGCGTTCTCTTCATCGACCATATTTCGCGGCTGAAGCGCGACATGGTCATCAAG
AAATTCACGAAAGCGGCCCGCGCAAAGATCTGA

Upstream 100 bases:

>100_bases
CTTCTGCGGCGGAAATGAGTCGGGCAGGGTCGATGCAGGAGGCGGTTTTCTCCTTCGTGTTTTTTATTTCCTCCGCGCGA
GAGATACGTTATGGGAAGCC

Downstream 100 bases:

>100_bases
TCCGTCCTTTTGGGCCGGAACGGACGACAGGGCAGGGGGCTACGATGTCTCTTCGCATCATTTTTATGGGCACGCCGGAT
TTCTCCGTTCCCACATTGCG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MTIKPLIILPDPVLRQQSKLIEQVDAEVLRLADDMLETMYDAPGIGLAAIQIGVPRRMLVIDVAREGEEKTPVVFINPEI
LKVSDDISTYEEGCLSIPDYYAEVERPASLTVQYVGRDGKQQTVEADGLLATCLQHEIDHLNGVLFIDHISRLKRDMVIK
KFTKAARAKI

Sequences:

>Translated_170_residues
MTIKPLIILPDPVLRQQSKLIEQVDAEVLRLADDMLETMYDAPGIGLAAIQIGVPRRMLVIDVAREGEEKTPVVFINPEI
LKVSDDISTYEEGCLSIPDYYAEVERPASLTVQYVGRDGKQQTVEADGLLATCLQHEIDHLNGVLFIDHISRLKRDMVIK
KFTKAARAKI
>Mature_169_residues
TIKPLIILPDPVLRQQSKLIEQVDAEVLRLADDMLETMYDAPGIGLAAIQIGVPRRMLVIDVAREGEEKTPVVFINPEIL
KVSDDISTYEEGCLSIPDYYAEVERPASLTVQYVGRDGKQQTVEADGLLATCLQHEIDHLNGVLFIDHISRLKRDMVIKK
FTKAARAKI

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=157, Percent_Identity=32.484076433121, Blast_Score=82, Evalue=3e-16,
Organism=Escherichia coli, GI1789682, Length=169, Percent_Identity=46.1538461538462, Blast_Score=146, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24645728, Length=177, Percent_Identity=32.2033898305085, Blast_Score=96, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24645726, Length=159, Percent_Identity=31.4465408805031, Blast_Score=80, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_AGRT5 (Q8UID1)

Other databases:

- EMBL:   AE007869
- PIR:   AF2621
- PIR:   F97403
- RefSeq:   NP_353398.1
- ProteinModelPortal:   Q8UID1
- SMR:   Q8UID1
- STRING:   Q8UID1
- GeneID:   1132404
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0366
- eggNOG:   COG0242
- HOGENOM:   HBG665227
- OMA:   MVIKKFT
- PhylomeDB:   Q8UID1
- ProtClustDB:   PRK00150
- BioCyc:   ATUM176299-1:ATU0366-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 19026; Mature: 18895

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: ACT_SITE 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKPLIILPDPVLRQQSKLIEQVDAEVLRLADDMLETMYDAPGIGLAAIQIGVPRRMLV
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEE
IDVAREGEEKTPVVFINPEILKVSDDISTYEEGCLSIPDYYAEVERPASLTVQYVGRDGK
EEECCCCCCCCCEEEECCCEEEECCCHHHHHHCCCCCCHHHHHCCCCHHEEEEEECCCCC
QQTVEADGLLATCLQHEIDHLNGVLFIDHISRLKRDMVIKKFTKAARAKI
CHHEHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIKPLIILPDPVLRQQSKLIEQVDAEVLRLADDMLETMYDAPGIGLAAIQIGVPRRMLV
CCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEE
IDVAREGEEKTPVVFINPEILKVSDDISTYEEGCLSIPDYYAEVERPASLTVQYVGRDGK
EEECCCCCCCCCEEEECCCEEEECCCHHHHHHCCCCCCHHHHHCCCCHHEEEEEECCCCC
QQTVEADGLLATCLQHEIDHLNGVLFIDHISRLKRDMVIKKFTKAARAKI
CHHEHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194