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The map label for this gene is pheT

Identifier: 15887609

GI number: 15887609

Start: 255544

End: 257967

Strand: Direct

Name: pheT

Synonym: Atu0259

Alternate gene names: 15887609

Gene position: 255544-257967 (Clockwise)

Preceding gene: 159184234

Following gene: 15887611

Centisome position: 8.99

GC content: 61.39

Gene sequence:

>2424_bases
ATGAAATTCACTCTTTCCTGGCTGAAAGAGCATCTCGAAACGGATGCGACTCTGGACGAGATTTGCGAACGGCTGACGGC
GATCGGTCTTGAAGTCGAGGATGTCGATGACAAGGCGGCCTACAAGCCTTTCGTCATCGCTAAGGTCGTCTCCGCCGAAA
AACATCCGGAGGCGGACCGTCTCAAGGTGCTGATGGTGGATGCGGGTGACGGCAAGCCGGTGCAGATCGTCTGCGGCGCG
CCGAATGCGCGCGCCGGCCTCGTCGGCGCGCTGGCGCGTCCCGGCGTTTACGTTCCGGGCATCGATGTTACGCTTGCCGT
TGGCAAGATACGCGGCGTCGAAAGCCACGGCATGATGTGCTCCGAAAAGGAGCTTAACATTTCCGACGATCACGACGGCA
TCATCGATCTGCCGGAAGATGCGCCTGTCGGCACGTCTTTCGCCACCTATGCCGGTCTCGACGATCCGGTCATCGAGATC
AACCTGACACCCAACCGTCCGGATTGCACGTCGATCTACGGCATTGCCCGCGATCTCGCCGCTTCCGGCCTCGGCAAGCT
GAAGACGAAGGCTGCGCCCTCCTTCAAGGTGGAAGGCGCAACGCCCGTCGACGTGAAGCTGGAACTGGACGATGCGGCCC
TCTGCCCCGGCTTTTCCCTGCGTATCGTGCGCGGCGTCAAGAACGGCCCGAGTCCGAAATGGATGCAGCAGCGCCTGATG
GCGATTGGCCTGCGTCCCATCAACGCGCTTGTTGACATCACCAACTACATGACCTTCGATCAGGGCCGGCCGATGCACGT
CTTCGACGCCGCCAAGGTCAAGGGTGATCTCACCGTGCGTCGCGCAAAAGACGGCGAGACGATCCTCGCGCTCGATCAGC
GCGAATACAAGCTCGGCCCGAACAATGTCGTCATCGCCGACGAAAAGGGCCTCGAGTCCATCGGCGGCGTCATGGGCGGT
GAACATTCCGGCTGCGACGAAAACACCATCGACGTGCTGATCGAATCCGCTCTCTGGGATCCGATCAACATTGCCAAGAC
CGGCCGTTCGCTCGGCATCATCACCGATGCGCGTTATCGGTTCGAGCGCGGCGTCGATCCGGAATATATGGTTCCGGGTC
TCGAACGCACCACCGAACTGGTTCTGGAACTTTGCGGCGGCATCGCTGGCGAAGCCAAGATCGTCGGTTACAAGGGGCAT
CAGCCCAAGGTCGTGGATTTTCCGCTGTCGGAAGTGAAGCGCCTGACCGGTCTCGAAGTTTCGTCGGAAGAAAGCCTCGC
CATTCTCAAAGGCCTCGGTTTTGGCGTCGAAGGAACGGGCGAGCGCGTATCCGTTACCGTCCCGTCCTGGCGTCCTGACG
TCGACGGCAAGGCTGATCTGGTCGAAGAAGTCATGCGCATCCACGGCGTTGACAATATCAAGCCGGAACCGCTGGAAAAC
TTCGGTGCCGTCAACGGCCGTATCCTGACCACGCTGCAGATCCGCACCCGTACTGCGCGCCGTGCCTTGGCAAGCCGTGG
CATGCTGGAGGCCGTCACATGGTCCTTCATCCCGGAAGCGCAGGCGAAGCTTTTTAGCGGCGGTTCGCCCGCGCTGAAAC
TGGCCAATCCCATCGCGGCCGACATGTCGGATATGCGGCCCTCGCTGCTGCCGGGCCTTCTCACCGCCGCCCAGCGCAAT
GCCGACAAGGGCCATGGCGACGTGGCGATCTTCGAGGTTTCCGGCACCTATGAGGGCGATACGCCTGAGAAGCAGCGTCG
CGTTGCCGGCGGTATCCGCCGTGGCACGGCCTCGCTCGCTGGCAGCGGCCGCATGTGGTCCAACGCGGCCAAGGGTGGCG
GCAAGCCGGTGGATGTCTATGATGCCAAGGCCGATGCTCTCTCGGTGCTGGAAGCCTGCGGCCTGCCCATGGCCAATGTG
CAGATCGAAGCAGGCGCTCCCGCCTGGTATCACCCCGGCCGTTCCGGCACCATCAAGATGGGTCCGAAGGTCGTGCTCGG
TTATTTCGGTGAGTTCCATCCCAAGACGCTGTCCGAGCTGGATGTTTCTGGCACCTATTGCGGTTTCGAGATTTATCTCG
ACGCCATGCCAGAGCCGAAGAAGAAAGCGACCCGCACCAAGCCTGCACTCGAGCTTTCGCCGTTCCAGGCGGTCAAGCGC
GACTTCGCTTTCGTGGTCGACAAGTCGGTGGAAGCAGGAGCCATCATCAAGGCCGCAACGAGCGCTGACCGAAAGCTGAT
CACCGGCGTCAACGTATTCGACATCTTCGAAGGCGCATCGCTCGGCGAAAACCGCAAGTCGGTGGCCATCGAGGTCCAGA
TCCAGCCAGTCGACAAGACGCTGACCGACGAGGATTTCGAAGCCTTGACGGCCAAGATCGTTGGCAATGTCGAGAAATCG
ACGGGCGGCGTTCTACGCGCCTGA

Upstream 100 bases:

>100_bases
CGATGTCCGCTGGATGAACCATTACGGCTTCCGCCCGCTCGACATGCCGACGCTGTTCGGCGGTCTGAGCGTTTAATCCG
GTTAGCCAAGGAGCAACGAC

Downstream 100 bases:

>100_bases
AAGCCTGAGAGCGGATGATAGAATGAAAAAAGCCGCGGCAGTTGCCGCGGCTTTTCTTTTTAGCGGTTGGCCATCTGCGA
CATGCGTTCGGGATATCGTT

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS

Number of amino acids: Translated: 807; Mature: 807

Protein sequence:

>807_residues
MKFTLSWLKEHLETDATLDEICERLTAIGLEVEDVDDKAAYKPFVIAKVVSAEKHPEADRLKVLMVDAGDGKPVQIVCGA
PNARAGLVGALARPGVYVPGIDVTLAVGKIRGVESHGMMCSEKELNISDDHDGIIDLPEDAPVGTSFATYAGLDDPVIEI
NLTPNRPDCTSIYGIARDLAASGLGKLKTKAAPSFKVEGATPVDVKLELDDAALCPGFSLRIVRGVKNGPSPKWMQQRLM
AIGLRPINALVDITNYMTFDQGRPMHVFDAAKVKGDLTVRRAKDGETILALDQREYKLGPNNVVIADEKGLESIGGVMGG
EHSGCDENTIDVLIESALWDPINIAKTGRSLGIITDARYRFERGVDPEYMVPGLERTTELVLELCGGIAGEAKIVGYKGH
QPKVVDFPLSEVKRLTGLEVSSEESLAILKGLGFGVEGTGERVSVTVPSWRPDVDGKADLVEEVMRIHGVDNIKPEPLEN
FGAVNGRILTTLQIRTRTARRALASRGMLEAVTWSFIPEAQAKLFSGGSPALKLANPIAADMSDMRPSLLPGLLTAAQRN
ADKGHGDVAIFEVSGTYEGDTPEKQRRVAGGIRRGTASLAGSGRMWSNAAKGGGKPVDVYDAKADALSVLEACGLPMANV
QIEAGAPAWYHPGRSGTIKMGPKVVLGYFGEFHPKTLSELDVSGTYCGFEIYLDAMPEPKKKATRTKPALELSPFQAVKR
DFAFVVDKSVEAGAIIKAATSADRKLITGVNVFDIFEGASLGENRKSVAIEVQIQPVDKTLTDEDFEALTAKIVGNVEKS
TGGVLRA

Sequences:

>Translated_807_residues
MKFTLSWLKEHLETDATLDEICERLTAIGLEVEDVDDKAAYKPFVIAKVVSAEKHPEADRLKVLMVDAGDGKPVQIVCGA
PNARAGLVGALARPGVYVPGIDVTLAVGKIRGVESHGMMCSEKELNISDDHDGIIDLPEDAPVGTSFATYAGLDDPVIEI
NLTPNRPDCTSIYGIARDLAASGLGKLKTKAAPSFKVEGATPVDVKLELDDAALCPGFSLRIVRGVKNGPSPKWMQQRLM
AIGLRPINALVDITNYMTFDQGRPMHVFDAAKVKGDLTVRRAKDGETILALDQREYKLGPNNVVIADEKGLESIGGVMGG
EHSGCDENTIDVLIESALWDPINIAKTGRSLGIITDARYRFERGVDPEYMVPGLERTTELVLELCGGIAGEAKIVGYKGH
QPKVVDFPLSEVKRLTGLEVSSEESLAILKGLGFGVEGTGERVSVTVPSWRPDVDGKADLVEEVMRIHGVDNIKPEPLEN
FGAVNGRILTTLQIRTRTARRALASRGMLEAVTWSFIPEAQAKLFSGGSPALKLANPIAADMSDMRPSLLPGLLTAAQRN
ADKGHGDVAIFEVSGTYEGDTPEKQRRVAGGIRRGTASLAGSGRMWSNAAKGGGKPVDVYDAKADALSVLEACGLPMANV
QIEAGAPAWYHPGRSGTIKMGPKVVLGYFGEFHPKTLSELDVSGTYCGFEIYLDAMPEPKKKATRTKPALELSPFQAVKR
DFAFVVDKSVEAGAIIKAATSADRKLITGVNVFDIFEGASLGENRKSVAIEVQIQPVDKTLTDEDFEALTAKIVGNVEKS
TGGVLRA
>Mature_807_residues
MKFTLSWLKEHLETDATLDEICERLTAIGLEVEDVDDKAAYKPFVIAKVVSAEKHPEADRLKVLMVDAGDGKPVQIVCGA
PNARAGLVGALARPGVYVPGIDVTLAVGKIRGVESHGMMCSEKELNISDDHDGIIDLPEDAPVGTSFATYAGLDDPVIEI
NLTPNRPDCTSIYGIARDLAASGLGKLKTKAAPSFKVEGATPVDVKLELDDAALCPGFSLRIVRGVKNGPSPKWMQQRLM
AIGLRPINALVDITNYMTFDQGRPMHVFDAAKVKGDLTVRRAKDGETILALDQREYKLGPNNVVIADEKGLESIGGVMGG
EHSGCDENTIDVLIESALWDPINIAKTGRSLGIITDARYRFERGVDPEYMVPGLERTTELVLELCGGIAGEAKIVGYKGH
QPKVVDFPLSEVKRLTGLEVSSEESLAILKGLGFGVEGTGERVSVTVPSWRPDVDGKADLVEEVMRIHGVDNIKPEPLEN
FGAVNGRILTTLQIRTRTARRALASRGMLEAVTWSFIPEAQAKLFSGGSPALKLANPIAADMSDMRPSLLPGLLTAAQRN
ADKGHGDVAIFEVSGTYEGDTPEKQRRVAGGIRRGTASLAGSGRMWSNAAKGGGKPVDVYDAKADALSVLEACGLPMANV
QIEAGAPAWYHPGRSGTIKMGPKVVLGYFGEFHPKTLSELDVSGTYCGFEIYLDAMPEPKKKATRTKPALELSPFQAVKR
DFAFVVDKSVEAGAIIKAATSADRKLITGVNVFDIFEGASLGENRKSVAIEVQIQPVDKTLTDEDFEALTAKIVGNVEKS
TGGVLRA

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Escherichia coli, GI1788006, Length=817, Percent_Identity=37.2093023255814, Blast_Score=468, Evalue=1e-133,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFB_AGRT5 (Q8UIN4)

Other databases:

- EMBL:   AE007869
- PIR:   AC2608
- PIR:   B97390
- RefSeq:   NP_353290.1
- ProteinModelPortal:   Q8UIN4
- STRING:   Q8UIN4
- GeneID:   1132297
- GenomeReviews:   AE007869_GR
- KEGG:   atu:Atu0259
- eggNOG:   COG0072
- HOGENOM:   HBG399638
- OMA:   ADKLRVC
- PhylomeDB:   Q8UIN4
- ProtClustDB:   PRK00629
- BioCyc:   ATUM176299-1:ATU0259-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00283
- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547
- Gene3D:   G3DSA:3.50.40.10
- Gene3D:   G3DSA:3.30.56.20
- Gene3D:   G3DSA:3.30.70.380
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00873
- SMART:   SM00874
- SMART:   SM00896
- TIGRFAMs:   TIGR00472

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind; SSF56037 B3_4; SSF54991 Fdx_AntiC_bd; SSF50249 Nucleic_acid_OB; SSF46955 Putativ_DNA_bind

EC number: =6.1.1.20

Molecular weight: Translated: 86358; Mature: 86358

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS51483 B5; PS51447 FDX_ACB; PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTLSWLKEHLETDATLDEICERLTAIGLEVEDVDDKAAYKPFVIAKVVSAEKHPEADR
CCEEHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHCCCCCCCCC
LKVLMVDAGDGKPVQIVCGAPNARAGLVGALARPGVYVPGIDVTLAVGKIRGVESHGMMC
EEEEEEECCCCCEEEEEECCCCCCCHHHHHHCCCCEEECCCCEEEEEEEECCCHHCCCEE
SEKELNISDDHDGIIDLPEDAPVGTSFATYAGLDDPVIEINLTPNRPDCTSIYGIARDLA
CCCCCCCCCCCCCEEECCCCCCCCCCHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHH
ASGLGKLKTKAAPSFKVEGATPVDVKLELDDAALCPGFSLRIVRGVKNGPSPKWMQQRLM
HHCCCHHHHCCCCCEEECCCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHH
AIGLRPINALVDITNYMTFDQGRPMHVFDAAKVKGDLTVRRAKDGETILALDQREYKLGP
HHCCCHHHHHHHHHHHEEECCCCCEEEEEHHHCCCCEEEEECCCCCEEEEECCCCEEECC
NNVVIADEKGLESIGGVMGGEHSGCDENTIDVLIESALWDPINIAKTGRSLGIITDARYR
CCEEEECCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHCCCEEEEEECCHHH
FERGVDPEYMVPGLERTTELVLELCGGIAGEAKIVGYKGHQPKVVDFPLSEVKRLTGLEV
HHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEECCHHHHHHHHCCCC
SSEESLAILKGLGFGVEGTGERVSVTVPSWRPDVDGKADLVEEVMRIHGVDNIKPEPLEN
CCCCCEEHHHHCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHH
FGAVNGRILTTLQIRTRTARRALASRGMLEAVTWSFIPEAQAKLFSGGSPALKLANPIAA
CCCCCCEEEEEEEEHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHCCCCCCEEECCCHHH
DMSDMRPSLLPGLLTAAQRNADKGHGDVAIFEVSGTYEGDTPEKQRRVAGGIRRGTASLA
CHHHCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHC
GSGRMWSNAAKGGGKPVDVYDAKADALSVLEACGLPMANVQIEAGAPAWYHPGRSGTIKM
CCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCEEEEECCCCCEECCCCCCEEEE
GPKVVLGYFGEFHPKTLSELDVSGTYCGFEIYLDAMPEPKKKATRTKPALELSPFQAVKR
CCEEEEECCCCCCCCHHHHCCCCCEEEEEEEEEECCCCCHHHHHCCCCCCCCCHHHHHHH
DFAFVVDKSVEAGAIIKAATSADRKLITGVNVFDIFEGASLGENRKSVAIEVQIQPVDKT
HHHEEEECCCCCCEEEEECCCCCCEEEECCCHHHHHCCCCCCCCCCEEEEEEEEEECCCC
LTDEDFEALTAKIVGNVEKSTGGVLRA
CCCHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKFTLSWLKEHLETDATLDEICERLTAIGLEVEDVDDKAAYKPFVIAKVVSAEKHPEADR
CCEEHHHHHHHHCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHCCCCCCCCC
LKVLMVDAGDGKPVQIVCGAPNARAGLVGALARPGVYVPGIDVTLAVGKIRGVESHGMMC
EEEEEEECCCCCEEEEEECCCCCCCHHHHHHCCCCEEECCCCEEEEEEEECCCHHCCCEE
SEKELNISDDHDGIIDLPEDAPVGTSFATYAGLDDPVIEINLTPNRPDCTSIYGIARDLA
CCCCCCCCCCCCCEEECCCCCCCCCCHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHH
ASGLGKLKTKAAPSFKVEGATPVDVKLELDDAALCPGFSLRIVRGVKNGPSPKWMQQRLM
HHCCCHHHHCCCCCEEECCCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCHHHHHHHHH
AIGLRPINALVDITNYMTFDQGRPMHVFDAAKVKGDLTVRRAKDGETILALDQREYKLGP
HHCCCHHHHHHHHHHHEEECCCCCEEEEEHHHCCCCEEEEECCCCCEEEEECCCCEEECC
NNVVIADEKGLESIGGVMGGEHSGCDENTIDVLIESALWDPINIAKTGRSLGIITDARYR
CCEEEECCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHCCCEEEEEECCHHH
FERGVDPEYMVPGLERTTELVLELCGGIAGEAKIVGYKGHQPKVVDFPLSEVKRLTGLEV
HHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEECCHHHHHHHHCCCC
SSEESLAILKGLGFGVEGTGERVSVTVPSWRPDVDGKADLVEEVMRIHGVDNIKPEPLEN
CCCCCEEHHHHCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHH
FGAVNGRILTTLQIRTRTARRALASRGMLEAVTWSFIPEAQAKLFSGGSPALKLANPIAA
CCCCCCEEEEEEEEHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHCCCCCCEEECCCHHH
DMSDMRPSLLPGLLTAAQRNADKGHGDVAIFEVSGTYEGDTPEKQRRVAGGIRRGTASLA
CHHHCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHC
GSGRMWSNAAKGGGKPVDVYDAKADALSVLEACGLPMANVQIEAGAPAWYHPGRSGTIKM
CCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCCEEEEECCCCCEECCCCCCEEEE
GPKVVLGYFGEFHPKTLSELDVSGTYCGFEIYLDAMPEPKKKATRTKPALELSPFQAVKR
CCEEEEECCCCCCCCHHHHCCCCCEEEEEEEEEECCCCCHHHHHCCCCCCCCCHHHHHHH
DFAFVVDKSVEAGAIIKAATSADRKLITGVNVFDIFEGASLGENRKSVAIEVQIQPVDKT
HHHEEEECCCCCCEEEEECCCCCCEEEECCCHHHHHCCCCCCCCCCEEEEEEEEEECCCC
LTDEDFEALTAKIVGNVEKSTGGVLRA
CCCHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194