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The map label for this gene is ahpF

Identifier: 57652638

GI number: 57652638

Start: 451793

End: 453316

Strand: Reverse

Name: ahpF

Synonym: SACOL0451

Alternate gene names: 57652638

Gene position: 453316-451793 (Counterclockwise)

Preceding gene: 57652639

Following gene: 57652637

Centisome position: 16.14

GC content: 37.93

Gene sequence:

>1524_bases
ATGCTTAATGCTGATTTAAAACAACAACTTAAACAACTATTAGAACTAATGGAGGGCAACGTTGAATTCGTTGCCAGCCT
TGGTTCAGATGATAAATCCAAAGAACTTAAAGATTTGTTGACAGAAATTACTGATATGTCACCTAGACTATCTCTTTCTG
AAAAATCTTTAAAACGTACACCAAGTTTCTCAGTCAATCGTCCTGGCGAAGAAACAGGTGTAACATTTGCAGGTATTCCA
TTAGGTCACGAGTTTAACTCACTTGTTTTAGCAATTTTACAGGTTAGTGGTCGTGCACCTAAAGAAAAACAGTCAATCAT
TGACCAAATTAAAAAATTAGAAGGTTCATTCCATTTTGAAACATTCATTAGTTTAACGTGTCAAAAATGTCCTGATGTCG
TTCAAGCACTTAACTTAATGAGTGTGATCAACCCTAACATCACGCATTCTATGATTGATGGTGCAGTGTTCCGTGAAGAA
TCTGAAAACATCATGGCAGTCCCTGCTGTCTTTTTAAATGGCGAAGAATTTGGCAATGGTCGTATGACAATCCAAGATAT
TCTTTCGAAACTAGGCAGTACGGCAGATGCATCTGAGTTTGAAAATAAAGAACCTTATGATGTCTTAATCGTTGGTGGTG
GTCCTGCTAGTGGTAGTGCAGCGATTTACACAGCACGTAAAGGTTTACGTACTGGTATAGTTGCTGATCGTATCGGTGGC
CAAGTTAATGATACTGCTGGTATTGAGAACTTCATTACTGTTAAAGAAACAACTGGTTCTGAATTTTCTTCTAACTTAGC
AGCGCACATTGATCAATATGACATTGATGCAATGACAGGTATACGTGCTACAGATATCGAAAAGACTGACGAAGCAATTA
AAGTTACGTTAGAAAACGGTGCTGTCTTAGAAAGTAAAACAGTCATTATTGCTACTGGTGCAGGTTGGCGTAAGCTAAAC
ATTCCAGGTGAAGAGCAATTGATTAATAAAGGTGTTGCATTCTGCCCTCACTGTGACGGACCTCTATTTGAAAATAAAGA
CGTAGCAGTTATCGGTGGCGGTAACTCTGGGGTTGAAGCAGCAATTGACCTTGCTGGTATCGTTAATCATGTTACATTAT
TCGAATTCGCTAGCGAATTAAAAGCAGACAACGTGTTACAAGATCGTTTACGTTCTTTATCAAATGTTGATATCAAAACA
AATGCCAAAACTACTGAAGTTGTCGGAGAAGACCATGTTACAGGTATACGTTACGAAGACATGAACACCGGCGAAGAACA
TCTACTTAACTTAGATGGTATCTTTGTTCAAATTGGTTTACTTCCAAACACATCATGGTTAAACGATGCTGTTGAATTAA
ACGAACGTGGTGAAATTGTGATTGATCGTAACAATAATACGAATGTTCCTGGAATATTTGCTGCTGGCGATGTCACAGAT
CAGAAGAACAAACAAATTATCATTTCAATGGGCGCTGGTGCAAATGCAGCATTAAATGCCTTTGACTATATTATCAGAAA
CTAA

Upstream 100 bases:

>100_bases
AAACCCTGGCGAAGTATGCCCAGCTAAATGGGAAGAAGGCGCTAAAACATTGCAACCTGGTTTAGATTTAGTAGGTAAAA
TCTAAGGAGGCATTACAACC

Downstream 100 bases:

>100_bases
AGTTTCATTCATTTCACTGACAGTATCTTACATTTGTTAAGATGCTGTTATTTTTTTATCCCGACATAAAATCATGTTAT
GATAAACTTAATATATACAA

Product: alkyl hydroperoxide reductase subunit F

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MLNADLKQQLKQLLELMEGNVEFVASLGSDDKSKELKDLLTEITDMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIP
LGHEFNSLVLAILQVSGRAPKEKQSIIDQIKKLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASGSAAIYTARKGLRTGIVADRIGG
QVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDIDAMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLN
IPGEEQLINKGVAFCPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRSLSNVDIKT
NAKTTEVVGEDHVTGIRYEDMNTGEEHLLNLDGIFVQIGLLPNTSWLNDAVELNERGEIVIDRNNNTNVPGIFAAGDVTD
QKNKQIIISMGAGANAALNAFDYIIRN

Sequences:

>Translated_507_residues
MLNADLKQQLKQLLELMEGNVEFVASLGSDDKSKELKDLLTEITDMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIP
LGHEFNSLVLAILQVSGRAPKEKQSIIDQIKKLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASGSAAIYTARKGLRTGIVADRIGG
QVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDIDAMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLN
IPGEEQLINKGVAFCPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRSLSNVDIKT
NAKTTEVVGEDHVTGIRYEDMNTGEEHLLNLDGIFVQIGLLPNTSWLNDAVELNERGEIVIDRNNNTNVPGIFAAGDVTD
QKNKQIIISMGAGANAALNAFDYIIRN
>Mature_507_residues
MLNADLKQQLKQLLELMEGNVEFVASLGSDDKSKELKDLLTEITDMSPRLSLSEKSLKRTPSFSVNRPGEETGVTFAGIP
LGHEFNSLVLAILQVSGRAPKEKQSIIDQIKKLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASGSAAIYTARKGLRTGIVADRIGG
QVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDIDAMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLN
IPGEEQLINKGVAFCPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRSLSNVDIKT
NAKTTEVVGEDHVTGIRYEDMNTGEEHLLNLDGIFVQIGLLPNTSWLNDAVELNERGEIVIDRNNNTNVPGIFAAGDVTD
QKNKQIIISMGAGANAALNAFDYIIRN

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family

Homologues:

Organism=Escherichia coli, GI87081763, Length=519, Percent_Identity=54.3352601156069, Blast_Score=548, Evalue=1e-157,
Organism=Escherichia coli, GI1787114, Length=310, Percent_Identity=34.8387096774194, Blast_Score=150, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6320560, Length=311, Percent_Identity=33.4405144694534, Blast_Score=153, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6321898, Length=312, Percent_Identity=31.7307692307692, Blast_Score=147, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AHPF_STAA8 (O05204)

Other databases:

- EMBL:   U92441
- EMBL:   CP000253
- RefSeq:   YP_498953.1
- ProteinModelPortal:   O05204
- STRING:   O05204
- EnsemblBacteria:   EBSTAT00000030290
- GeneID:   3919783
- GenomeReviews:   CP000253_GR
- KEGG:   sao:SAOUHSC_00364
- eggNOG:   COG3634
- GeneTree:   EBGT00050000023768
- HOGENOM:   HBG289531
- OMA:   MMAAINP
- ProtClustDB:   PRK15317
- BioCyc:   SAUR93061:SAOUHSC_00364-MONOMER
- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335
- Gene3D:   G3DSA:3.40.30.10
- PIRSF:   PIRSF000238
- PRINTS:   PR00368
- PRINTS:   PR00469
- TIGRFAMs:   TIGR03140

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; SSF52833 Thiordxn-like_fd

EC number: 1.6.4.- [C]

Molecular weight: Translated: 54721; Mature: 54721

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS51354 GLUTAREDOXIN_2; PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNADLKQQLKQLLELMEGNVEFVASLGSDDKSKELKDLLTEITDMSPRLSLSEKSLKRT
CCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHCC
PSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKEKQSIIDQIKKLEGSFHFE
CCCCCCCCCHHHCEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEHH
TFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREESENIMAVPAVFLNGEEFGNG
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHCCCCCEEEEEEEEECCCCCCCC
RMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASGSAAIYTARKGLRTGIVADRIGG
CEEHHHHHHHHCCCCCHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCCHHCHHHHHCCC
QVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDIDAMTGIRATDIEKTDEAIKVTLENG
CCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCHHHCCCEECCCCCCCCEEEEEECCC
AVLESKTVIIATGAGWRKLNIPGEEQLINKGVAFCPHCDGPLFENKDVAVIGGGNSGVEA
EEEECCEEEEEECCCEEEECCCCHHHHHHCCCEECCCCCCCCCCCCCEEEEECCCCCHHH
AIDLAGIVNHVTLFEFASELKADNVLQDRLRSLSNVDIKTNAKTTEVVGEDHVTGIRYED
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEECCCCHHEECCCCCCCCEEECC
MNTGEEHLLNLDGIFVQIGLLPNTSWLNDAVELNERGEIVIDRNNNTNVPGIFAAGDVTD
CCCCHHHEEECCCEEEEEEECCCCHHHHHHHEECCCCCEEEECCCCCCCCEEEEECCCCC
QKNKQIIISMGAGANAALNAFDYIIRN
CCCCEEEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MLNADLKQQLKQLLELMEGNVEFVASLGSDDKSKELKDLLTEITDMSPRLSLSEKSLKRT
CCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHCC
PSFSVNRPGEETGVTFAGIPLGHEFNSLVLAILQVSGRAPKEKQSIIDQIKKLEGSFHFE
CCCCCCCCCHHHCEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEHH
TFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREESENIMAVPAVFLNGEEFGNG
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHCCCCCEEEEEEEEECCCCCCCC
RMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASGSAAIYTARKGLRTGIVADRIGG
CEEHHHHHHHHCCCCCHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCCHHCHHHHHCCC
QVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDIDAMTGIRATDIEKTDEAIKVTLENG
CCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCHHHCCCEECCCCCCCCEEEEEECCC
AVLESKTVIIATGAGWRKLNIPGEEQLINKGVAFCPHCDGPLFENKDVAVIGGGNSGVEA
EEEECCEEEEEECCCEEEECCCCHHHHHHCCCEECCCCCCCCCCCCCEEEEECCCCCHHH
AIDLAGIVNHVTLFEFASELKADNVLQDRLRSLSNVDIKTNAKTTEVVGEDHVTGIRYED
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEECCCCHHEECCCCCCCCEEECC
MNTGEEHLLNLDGIFVQIGLLPNTSWLNDAVELNERGEIVIDRNNNTNVPGIFAAGDVTD
CCCCHHHEEECCCEEEEEEECCCCHHHHHHHEECCCCCEEEECCCCCCCCEEEEECCCCC
QKNKQIIISMGAGANAALNAFDYIIRN
CCCCEEEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA