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The map label for this gene is yitJ [H]

Identifier: 57652617

GI number: 57652617

Start: 434214

End: 436055

Strand: Reverse

Name: yitJ [H]

Synonym: SACOL0429

Alternate gene names: 57652617

Gene position: 436055-434214 (Counterclockwise)

Preceding gene: 57652618

Following gene: 57652616

Centisome position: 15.52

GC content: 36.1

Gene sequence:

>1842_bases
ATGAGTCAATTCCTCACACAATTGAAAGATAATGTTTTAGTAGCTGATGGCGCTATAGGAACCATTTTATACTCTGAAGG
ATTAGACACCTGTCCAGAAGCATATAATCTTAGCCATCCAGATAAAGTTGAACGCATCCATCGTTCATATATTGAAGCCG
GTGCTGATGTCATTCAAACCAATACTTATGGTGCAAATTTTGAAAAGTTAAAACGATTCGGTCTTGAAGATAAAGTTAAA
GCAATACATCAAGCCGCCGTTCGCATCGCAAAAAAAGCAGCAAATAAAGATACGTATATATTAGGCACAGTTGGTGGGTT
TAGAGGTATCAAACAAGAGGATATCAGCTTACAAACTATTCTTTATCATACTGAAATTCAAATAGACACCTTAATTGAAG
AAGGCGTTGACGCGCTACTTTTCGAAACGTATTACGACCTAGAAGAGTTAACAAATGTCATTTCACGAACGAGAAAGAAA
TACGACATTCCAATCATTGCTCAATTAACCGCTTCAAACACAAATTACTTAGTTAATGGTCAGGCAATCAATGAAGGATT
AAAACAACTCGTTCAATGTGGTGCAAACATCGTGGGACTCAATTGTCATCATGGCCCGCACCATATGCAAGAGTCTTTCA
CACATATTGAATTACCAGAGCACGCATTCTTATCTTGTTATCCAAATGCCAGCTTATTAGATATTGAAAATAGTGAATTT
AAGTATAGTGACAATGCACAATATTTCGGTCAAGTTGCTCAAAATCTAATTCGCGAAGGTGTTCGTTTAATTGGTGGTTG
CTGTGGTACAACGCCAGAGCACATCAAATTTATTAAAGAATCTATTCAGACACTTAAACCTGTTAATGACAAAAAAGTGA
TTCCGATACCAACGAAAGCACTTTTCAATCCATCTCAAAATAAAGTTAGACAATCATTAACATCTAAGGTTCAAGAACGT
CCAACCGTTATTATCGAATTGGATACACCGAAACATTTAGACACGGATAGATTTTTTGAAAATATCGCTAAACTTGATAA
AGCTAATGTAGATGCGGTAACACTCGCAGATAATTCATTGGCAACTGTCAGAATTAGCAATATTGCTGCTGCTAGCTTAA
TTAAGCAATATTACAATATTGAACCACTCGTACATATTACATGTCGAGACCGAAACTTAATCGGCTTGCAGTCCCATTTA
CTTGGATTATCGCTCATTGGCGTTAACGAAATATTAGCCATAACTGGTGATCCTTCAAAAGTTGGTCACTTACCAGGTGC
AACCAATGTCTATGATGTTAATTCTAAAGGATTAACTGAACTCGCTCTAAGATTTAATCAAGGTATTAACACTGACGGTG
ATGCGCTGAAGAAACGTACACACTTCAACATCGCTGGCGCCTTTAACCCTAATGTTCGTAAATTATATGGTGCCGTCAAA
AGATTAGAGAAAAAGATAGAAAGCGGAATGTCTTATTTTATAACACAACCCGTGTACAGCAAAGAGAAAATCATTGAAAT
TTACCATGCCACTAAGCACTTGAACAAACCATTTTTCATAGGCATTATGCCTATCGCAAGTTACAAAAACGCACTCTTTT
TGCATAATGAAGTGCCAGGAATCAAGATGTCAGATGAAATTTTACAACAATTTGAAGCAGTTAAAGATGATAAAGCCAAA
ACACGAGAACTAAGTCTTAAGCTTTCAAAGGATTTAATCGATACTGTTCATGAATATTTTAATGGTTTATACATTATCAC
ACCGTTTCAAAATGTCGAAGATTCATTAGAACTTGCAGCATACTCAAAATCTATTACTGCTCACAAGGAGGCAATATTAT
GA

Upstream 100 bases:

>100_bases
GAACGTGGTATCACTTTCGGTCTATTCCGATTAAGTGTTGGTCTCGAAGATCCTGATGAACTCATTGCAGACATCAAATA
CGCATTAAAGGAGGCATTCA

Downstream 100 bases:

>100_bases
CAACAATTAAAACATCAAACTTAGGATTCCCAAGATTAGGTAGAAAAAGAGAATGGAAAAAAGCCATCGAAAGTTATTGG
GCAAAGAAGATTTCTAAAGA

Product: bifunctional homocysteine S-methyltransferase/5,10-methylenetetrahydrofolate reductase protein

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 613; Mature: 612

Protein sequence:

>613_residues
MSQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVERIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVK
AIHQAAVRIAKKAANKDTYILGTVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKK
YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIELPEHAFLSCYPNASLLDIENSEF
KYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIKFIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQER
PTVIIELDTPKHLDTDRFFENIAKLDKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRNLIGLQSHL
LGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGINTDGDALKKRTHFNIAGAFNPNVRKLYGAVK
RLEKKIESGMSYFITQPVYSKEKIIEIYHATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDKAK
TRELSLKLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL

Sequences:

>Translated_613_residues
MSQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVERIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVK
AIHQAAVRIAKKAANKDTYILGTVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKK
YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIELPEHAFLSCYPNASLLDIENSEF
KYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIKFIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQER
PTVIIELDTPKHLDTDRFFENIAKLDKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRNLIGLQSHL
LGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGINTDGDALKKRTHFNIAGAFNPNVRKLYGAVK
RLEKKIESGMSYFITQPVYSKEKIIEIYHATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDKAK
TRELSLKLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL
>Mature_612_residues
SQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVERIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKA
IHQAAVRIAKKAANKDTYILGTVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKKY
DIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIELPEHAFLSCYPNASLLDIENSEFK
YSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIKFIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQERP
TVIIELDTPKHLDTDRFFENIAKLDKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRNLIGLQSHLL
GLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGINTDGDALKKRTHFNIAGAFNPNVRKLYGAVKR
LEKKIESGMSYFITQPVYSKEKIIEIYHATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDKAKT
RELSLKLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL

Specific function: Terminal step in the de novo biosynthesis of methionine. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=323, Percent_Identity=26.625386996904, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI157266337, Length=331, Percent_Identity=26.5861027190332, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI13162290, Length=311, Percent_Identity=26.6881028938907, Blast_Score=83, Evalue=1e-15,
Organism=Escherichia coli, GI1790450, Length=430, Percent_Identity=24.6511627906977, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1786456, Length=309, Percent_Identity=25.5663430420712, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17535405, Length=321, Percent_Identity=25.5451713395639, Blast_Score=112, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6324982, Length=323, Percent_Identity=22.6006191950464, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 68479; Mature: 68348

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVERIHRSYIEAGADVIQT
CCHHHHHHCCCEEEECCCCEEEEECCCCCCCCHHCCCCCCHHHHHHHHHHHHCCCCEEEC
NTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAANKDTYILGTVGGFRGIKQEDISLQTI
CCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEE
LYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKKYDIPIIAQLTASNTNYLVNG
EEEEEEHHHHHHHHCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEC
QAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIELPEHAFLSCYPNASLLDIENSEF
HHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCEEECCCCCEEEECCCCEEEECCCCCC
KYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIKFIKESIQTLKPVNDKKVIPIPTKA
EECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEECCCHHH
LFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLDTDRFFENIAKLDKANVDAVTLADNSL
HCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCE
ATVRISNIAAASLIKQYYNIEPLVHITCRDRNLIGLQSHLLGLSLIGVNEILAITGDPSK
EEEEEHHHHHHHHHHHHHCCCCEEEEEECCCCEEHHHHHHHHEEEECCCEEEEEECCCHH
VGHLPGATNVYDVNSKGLTELALRFNQGINTDGDALKKRTHFNIAGAFNPNVRKLYGAVK
CCCCCCCCCEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHCEEEEECCCCHHHHHHHHHH
RLEKKIESGMSYFITQPVYSKEKIIEIYHATKHLNKPFFIGIMPIASYKNALFLHNEVPG
HHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHCCCCEEEEECCCCC
IKMSDEILQQFEAVKDDKAKTRELSLKLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAA
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHH
YSKSITAHKEAIL
HHHHHHHHHHHCC
>Mature Secondary Structure 
SQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVERIHRSYIEAGADVIQT
CHHHHHHCCCEEEECCCCEEEEECCCCCCCCHHCCCCCCHHHHHHHHHHHHCCCCEEEC
NTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAANKDTYILGTVGGFRGIKQEDISLQTI
CCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEEE
LYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKKYDIPIIAQLTASNTNYLVNG
EEEEEEHHHHHHHHCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEC
QAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIELPEHAFLSCYPNASLLDIENSEF
HHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCEEECCCCCEEEECCCCEEEECCCCCC
KYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIKFIKESIQTLKPVNDKKVIPIPTKA
EECCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEECCCHHH
LFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLDTDRFFENIAKLDKANVDAVTLADNSL
HCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCE
ATVRISNIAAASLIKQYYNIEPLVHITCRDRNLIGLQSHLLGLSLIGVNEILAITGDPSK
EEEEEHHHHHHHHHHHHHCCCCEEEEEECCCCEEHHHHHHHHEEEECCCEEEEEECCCHH
VGHLPGATNVYDVNSKGLTELALRFNQGINTDGDALKKRTHFNIAGAFNPNVRKLYGAVK
CCCCCCCCCEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHCEEEEECCCCHHHHHHHHHH
RLEKKIESGMSYFITQPVYSKEKIIEIYHATKHLNKPFFIGIMPIASYKNALFLHNEVPG
HHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHCCCCEEEEECCCCC
IKMSDEILQQFEAVKDDKAKTRELSLKLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAA
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHH
YSKSITAHKEAIL
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]