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The map label for this gene is metE [H]

Identifier: 57652616

GI number: 57652616

Start: 431989

End: 434217

Strand: Reverse

Name: metE [H]

Synonym: SACOL0428

Alternate gene names: 57652616

Gene position: 434217-431989 (Counterclockwise)

Preceding gene: 57652617

Following gene: 57652615

Centisome position: 15.46

GC content: 34.1

Gene sequence:

>2229_bases
ATGACAACAATTAAAACATCAAACTTAGGATTCCCAAGATTAGGTAGAAAAAGAGAATGGAAAAAAGCCATCGAAAGTTA
TTGGGCAAAGAAGATTTCTAAAGAAGAATTAGATCAAACATTAACTGATCTACATAAAGAAAATTTATTATTACAAAAGT
ACTATCACTTAGATAGCATCCCAGTTGGTGATTTCTCTTTATATGACCATATATTAGATACGTCATTATTATTCAACATC
ATCCCTGAACGTTTCCAAGGAAGAACTATTGATGATGATTTATTATTTGATATTGCTCGCGGTAATAAAGACCATGTTGC
AAGTGCACTTATAAAATGGTTTAACACTAACTATCATTACATCGTACCTGAATGGGACAATGTTGAGCCTAAAGTGAGTC
GCAATGTATTATTAGATCGCTTTAAATATGCCCAATCTTTAAACGTTAACGCTCACCCTGTCATTGTTGGTCCGATTACT
TTTGTTAAATTATCAAAAGGCGGTCACCAAACTTTTGAAGAAAAAGTTAAAACGTTATTACCACTTTATAAAGAAGTGTT
TGAATCATTAATTGATGCAGGTGCTGAGTACATTCAAGTTGATGAGCCAATCTTAGTTACAGACGACAGCGAAAGCTATG
AAAATATTACACGTGAAGCTTATGACTATTTCGAAAAAGCTGGTGTTGCTAAAAAATTAGTCATTCAAACATACTTTGAA
CGTGCACATCTTAAATTCTTAAGTTCTTTACCTGTTGGTGGTATAGGTTTAGACTTTGTCCATGATAATGGCTATAACCT
TAAACAAATTGAAGCTGGAGATTTTGATAAATCAAAAACATTATACGCTGGAATTATTGATGGTCGTAATGTATGGGCAA
GTGACATTGAAGCTAAAAAAGTCTTAATCGATAAATTGTTAGCACACACTAATGAACTTGTCATTCAACCATCATCTTCA
TTATTACATGTTCCAGTATCTTTAGATGATGAAACATTAGATACAAGTGTTGGAGAAGGCTTAAGCTTTGCAACTGAAAA
ATTAGACGAATTAGATGCATTGCGTCGCCTATTTAATCAAAACGATAGTGTTAAATATGATAAATTAAAAGCACGTTATG
AGCGTTTCCAAAATCAATCATTCAAAAACTTAGATTATGATTTCGAAAGCGTGCGTACTTCAAGACAATCACCATTCGCG
CAACGTATTGAACAACAACAAAAACGTTTGAACTTACCGGATTTACCAACAACAACTATTGGATCATTCCCACAAAGCCG
AGAAGTTCGAAAATACCGTGCAGATTGGAAGAACAAACGCATTACAGACGAAGCATATGAAACATTCTTAAAAAATGAAA
TTGCTCGATGGATTAAAATTCAAGAAGACATTGGCTTAGATGTATTAGTTCACGGTGAATTTGAACGTAATGACATGGTT
GAATTCTTCGGAGAAAAATTACAAGGTTTCTTAGTAACTAAATTCGGTTGGGTGCAATCATATGGTTCACGTGCCGTAAA
ACCACCAATCATTTATGGTGATGTAAAATGGACAGCGCCTTTAACTGTTGATGAAACAGTTTATGCACAAAGCTTAACAG
ATAAACCAGTTAAAGGTATGTTAACTGGACCTGTAACAATTCTAAACTGGTCATTTGAACGTGTTGATTTACCACGTAAA
GTCGTTCAAGATCAAATTGCTTTAGCAATCAACGAAGAAGTATTAGCACTTGAAGCTGCAGGAATCAAAGTTATCCAAGT
TGACGAACCTGCATTACGTGAAGGCTTACCATTACGCTCTGAATATCACGAACAATATCTTAAAGATGCTGTTTTATCAT
TTAAACTTGCAACGTCTTCAGTTCGTGATGAAACTCAAATCCATACACATATGTGTTATTCTCAATTCGGTCAAATCATT
CATGCTATTCATGACTTAGATGCTGATGTTATTTCAATTGAAACATCTCGTAGCCATGGTGATTTAATTAAAGACTTTGA
AGATATTAATTATGATTTAGGTATTGGTCTAGGTGTATATGATATTCATAGTCCACGTATTCCAACAAAAGAAGAAATTA
CTACAGCAATCAATCGTTCACTACAACAAATCGACCGCTCATTATTCTGGGTAAACCCTGACTGTGGTTTAAAAACGCGA
AAAGAAGAAGAAGTTAAAGATGCATTGACTGTGCTTGTGAATGCTGTTAAAGCTAAACGCCAAGAATAA

Upstream 100 bases:

>100_bases
TTAATGGTTTATACATTATCACACCGTTTCAAAATGTCGAAGATTCATTAGAACTTGCAGCATACTCAAAATCTATTACT
GCTCACAAGGAGGCAATATT

Downstream 100 bases:

>100_bases
TCGCAGCATTTATCATATAATAAACTAAAAACGGGTGATTTTATGACACAATATCCTTTATGGCAACAATTAAATACTTT
AAAACAAGCAACTTGGGTTG

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 742; Mature: 741

Protein sequence:

>742_residues
MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDLHKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNI
IPERFQGRTIDDDLLFDIARGNKDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITREAYDYFEKAGVAKKLVIQTYFE
RAHLKFLSSLPVGGIGLDFVHDNGYNLKQIEAGDFDKSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSS
LLHVPVSLDDETLDTSVGEGLSFATEKLDELDALRRLFNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVRTSRQSPFA
QRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFLKNEIARWIKIQEDIGLDVLVHGEFERNDMV
EFFGEKLQGFLVTKFGWVQSYGSRAVKPPIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRK
VVQDQIALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDETQIHTHMCYSQFGQII
HAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDIHSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTR
KEEEVKDALTVLVNAVKAKRQE

Sequences:

>Translated_742_residues
MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDLHKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNI
IPERFQGRTIDDDLLFDIARGNKDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITREAYDYFEKAGVAKKLVIQTYFE
RAHLKFLSSLPVGGIGLDFVHDNGYNLKQIEAGDFDKSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSS
LLHVPVSLDDETLDTSVGEGLSFATEKLDELDALRRLFNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVRTSRQSPFA
QRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFLKNEIARWIKIQEDIGLDVLVHGEFERNDMV
EFFGEKLQGFLVTKFGWVQSYGSRAVKPPIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRK
VVQDQIALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDETQIHTHMCYSQFGQII
HAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDIHSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTR
KEEEVKDALTVLVNAVKAKRQE
>Mature_741_residues
TTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDLHKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNII
PERFQGRTIDDDLLFDIARGNKDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPITF
VKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITREAYDYFEKAGVAKKLVIQTYFER
AHLKFLSSLPVGGIGLDFVHDNGYNLKQIEAGDFDKSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSL
LHVPVSLDDETLDTSVGEGLSFATEKLDELDALRRLFNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVRTSRQSPFAQ
RIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFLKNEIARWIKIQEDIGLDVLVHGEFERNDMVE
FFGEKLQGFLVTKFGWVQSYGSRAVKPPIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKV
VQDQIALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDETQIHTHMCYSQFGQIIH
AIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDIHSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRK
EEEVKDALTVLVNAVKAKRQE

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=759, Percent_Identity=44.0052700922266, Blast_Score=597, Evalue=1e-172,
Organism=Saccharomyces cerevisiae, GI6320936, Length=769, Percent_Identity=42.3927178153446, Blast_Score=577, Evalue=1e-165,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 85076; Mature: 84945

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDLHKENLLLQKYYHLDSI
CCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
PVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGNKDHVASALIKWFNTNYHY
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCEE
IVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPITFVKLSKGGHQTFEEKVKTLL
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCEEEEEECCCCHHHHHHHHHHHH
PLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITREAYDYFEKAGVAKKLVIQTYFE
HHHHHHHHHHHHCCCCEEEECCCEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RAHLKFLSSLPVGGIGLDFVHDNGYNLKQIEAGDFDKSKTLYAGIIDGRNVWASDIEAKK
HHHHHHHHCCCCCCCEEEEEECCCCCEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHH
VLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLDTSVGEGLSFATEKLDELDALRRLFNQ
HHHHHHHHCCCCEEEECCCCEEEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
NDSVKYDKLKARYERFQNQSFKNLDYDFESVRTSRQSPFAQRIEQQQKRLNLPDLPTTTI
CCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCC
GSFPQSREVRKYRADWKNKRITDEAYETFLKNEIARWIKIQEDIGLDVLVHGEFERNDMV
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHH
EFFGEKLQGFLVTKFGWVQSYGSRAVKPPIIYGDVKWTAPLTVDETVYAQSLTDKPVKGM
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEECCCCCCHHHHHHHCCCCCCCCE
LTGPVTILNWSFERVDLPRKVVQDQIALAINEEVLALEAAGIKVIQVDEPALREGLPLRS
ECCCEEEEECCHHHHCCCHHHHHHHHHHHCCCHHEEEEECCEEEEEECCHHHHCCCCCHH
EYHEQYLKDAVLSFKLATSSVRDETQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
DLIKDFEDINYDLGIGLGVYDIHSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTR
HHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCC
KEEEVKDALTVLVNAVKAKRQE
CHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDLHKENLLLQKYYHLDSI
CEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
PVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGNKDHVASALIKWFNTNYHY
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCEE
IVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPITFVKLSKGGHQTFEEKVKTLL
ECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCEEEEEECCCCHHHHHHHHHHHH
PLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITREAYDYFEKAGVAKKLVIQTYFE
HHHHHHHHHHHHCCCCEEEECCCEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RAHLKFLSSLPVGGIGLDFVHDNGYNLKQIEAGDFDKSKTLYAGIIDGRNVWASDIEAKK
HHHHHHHHCCCCCCCEEEEEECCCCCEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHHH
VLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLDTSVGEGLSFATEKLDELDALRRLFNQ
HHHHHHHHCCCCEEEECCCCEEEEEECCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
NDSVKYDKLKARYERFQNQSFKNLDYDFESVRTSRQSPFAQRIEQQQKRLNLPDLPTTTI
CCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCC
GSFPQSREVRKYRADWKNKRITDEAYETFLKNEIARWIKIQEDIGLDVLVHGEFERNDMV
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHH
EFFGEKLQGFLVTKFGWVQSYGSRAVKPPIIYGDVKWTAPLTVDETVYAQSLTDKPVKGM
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCEEECCCCCCHHHHHHHCCCCCCCCE
LTGPVTILNWSFERVDLPRKVVQDQIALAINEEVLALEAAGIKVIQVDEPALREGLPLRS
ECCCEEEEECCHHHHCCCHHHHHHHHHHHCCCHHEEEEECCEEEEEECCHHHHCCCCCHH
EYHEQYLKDAVLSFKLATSSVRDETQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
DLIKDFEDINYDLGIGLGVYDIHSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTR
HHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCCC
KEEEVKDALTVLVNAVKAKRQE
CHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA