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The map label for this gene is purR [H]

Identifier: 57652531

GI number: 57652531

Start: 296295

End: 297293

Strand: Reverse

Name: purR [H]

Synonym: SACOL0257

Alternate gene names: 57652531

Gene position: 297293-296295 (Counterclockwise)

Preceding gene: 57652535

Following gene: 57652530

Centisome position: 10.58

GC content: 37.34

Gene sequence:

>999_bases
ATGAAAAAAGTGTCAATTAAAGATGTTGCTAGAGAAGCTGGTGTATCAGTTACAACTGTGTCACATATTTTAAATCATAA
TGATAGTCGTTTTTCCGCAACAACGATAAAAAACGTACATGCTGTTTCAGAACGTTTAGGCTATGCCCCTAATAAACATG
CAAAACAATTGCGCGGCAGTAAAATTCAAACTATTGGCGTCATTTTGCCTAGCTTAACAAATCCGTTTTTCTCAGCACTG
ATGCAAAGTATTCATGACCATAAACCATCTGATGTTGATTTATGCTTTTTAACATCTACAGCAACTGATTTGTATGACAA
TATTAAACATTTAATTGATCGAGGTATTGACGGATTAATTATCGCACAATACATATCATCCCCGGACGCCCTAAATAACT
ATCTAAAGAAACATCATGTACCTTATGTCGTACTGGATCAAAATGACCATCAAGGCTATACAGATTTTGTTCGGACAAAT
GAATATCAAGGTGGACAACTTGCAGCACAACATTTAATAGAACTCGGTCACAACCATATGATAATTGTTGCACCATATGA
CATGATGGCGAATATGTCGACTCGTGTCGCTGGATTTGTCGATACTTTGCGCGCGAATCAATTGCCAGAACCACAAATCG
TCCATACTGAATTATCTAAGCGCGGTGGGCTAACCATTGTTGATGACATCATGGTTCAATCTGCCACTGCAATCTTCGCT
ATTAACGATGAACTCGCTATTGGCATTTTACGAGGACTAATTGAACATGGCATCAGTATCCCGAAAGATATCTCATTAAT
AGGTTATGACGACATTGATTATGCAGCGTACGTCTCGCCACCTTTAACTACTGTGGCACAACCTATAACTGATATTGGCA
AAACATCTTTAACCTTATTACTTCAACGATTACAGCACTTAGATAAATCCATTGATATGATTGAATTACCAACGACTTTA
AAAATTCGTGCAACAACTGGCTATCATCTTTCAAACTAA

Upstream 100 bases:

>100_bases
CGTCTTCATATGTCATCAAATGTTTATCATGATATGATGAATATAATAATCGGGTATATAACTGTATGATTAATTACACA
ATAAAGAGAAGGAATATAAT

Downstream 100 bases:

>100_bases
CTACGTATCTTCCGAAATATACTCATCATTGTTAGGCCCTTAGCGTTGCTTTAATGCTGAGGGTTTTTAATCATAATTAT
TTTACTAAGAAATTAAAATA

Product: ribose operon repressor

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSERLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSAL
MQSIHDHKPSDVDLCFLTSTATDLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
EYQGGQLAAQHLIELGHNHMIIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTELSKRGGLTIVDDIMVQSATAIFA
INDELAIGILRGLIEHGISIPKDISLIGYDDIDYAAYVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTL
KIRATTGYHLSN

Sequences:

>Translated_332_residues
MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSERLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSAL
MQSIHDHKPSDVDLCFLTSTATDLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
EYQGGQLAAQHLIELGHNHMIIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTELSKRGGLTIVDDIMVQSATAIFA
INDELAIGILRGLIEHGISIPKDISLIGYDDIDYAAYVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTL
KIRATTGYHLSN
>Mature_332_residues
MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSERLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSAL
MQSIHDHKPSDVDLCFLTSTATDLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
EYQGGQLAAQHLIELGHNHMIIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTELSKRGGLTIVDDIMVQSATAIFA
INDELAIGILRGLIEHGISIPKDISLIGYDDIDYAAYVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTL
KIRATTGYHLSN

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=342, Percent_Identity=30.4093567251462, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1790194, Length=309, Percent_Identity=30.42071197411, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1786540, Length=332, Percent_Identity=30.7228915662651, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1788474, Length=307, Percent_Identity=31.2703583061889, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1787906, Length=321, Percent_Identity=26.791277258567, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1790369, Length=308, Percent_Identity=28.2467532467532, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1789202, Length=295, Percent_Identity=29.8305084745763, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789068, Length=297, Percent_Identity=28.6195286195286, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787580, Length=314, Percent_Identity=28.343949044586, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI48994940, Length=329, Percent_Identity=24.0121580547112, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1790715, Length=323, Percent_Identity=21.3622291021672, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786268, Length=319, Percent_Identity=21.6300940438871, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1789456, Length=343, Percent_Identity=25.6559766763848, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790689, Length=323, Percent_Identity=22.6006191950464, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36659; Mature: 36659

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSERLGYAPNKHAKQLRGS
CCCCCHHHHHHHHCCHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCCHHHHHHCCC
KIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTATDLYDNIKHLIDRGIDGLI
CEEEHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCEE
IAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTNEYQGGQLAAQHLIELGHNHM
HHHHHCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCE
IIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTELSKRGGLTIVDDIMVQSATAIFA
EEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHCCCCEEHHHHHHHCCEEEEE
INDELAIGILRGLIEHGISIPKDISLIGYDDIDYAAYVSPPLTTVAQPITDIGKTSLTLL
ECCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCEECCCCHHHHHHHHHHHHHHHHHHH
LQRLQHLDKSIDMIELPTTLKIRATTGYHLSN
HHHHHHHHHCCCEEECCCEEEEEEECCCCCCC
>Mature Secondary Structure
MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSERLGYAPNKHAKQLRGS
CCCCCHHHHHHHHCCHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCCHHHHHHCCC
KIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTATDLYDNIKHLIDRGIDGLI
CEEEHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCEE
IAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTNEYQGGQLAAQHLIELGHNHM
HHHHHCCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCE
IIVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTELSKRGGLTIVDDIMVQSATAIFA
EEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHCCCCEEHHHHHHHCCEEEEE
INDELAIGILRGLIEHGISIPKDISLIGYDDIDYAAYVSPPLTTVAQPITDIGKTSLTLL
ECCHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCEECCCCHHHHHHHHHHHHHHHHHHH
LQRLQHLDKSIDMIELPTTLKIRATTGYHLSN
HHHHHHHHHCCCEEECCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA