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The map label for this gene is norB [H]

Identifier: 57652535

GI number: 57652535

Start: 298738

End: 300114

Strand: Reverse

Name: norB [H]

Synonym: SACOL0261

Alternate gene names: 57652535

Gene position: 300114-298738 (Counterclockwise)

Preceding gene: 57652538

Following gene: 57652531

Centisome position: 10.68

GC content: 37.47

Gene sequence:

>1377_bases
GTGAATAACACACAATCTTCACCACGCAGTAATATTATTATTGCGATTATGTTGTCTGCATTAACATATTGGTTGTTTGC
ACAATCATTTATTAATATAGGACCTCTCGTTGGTCAAACGTATCAAACCTCTCCTGCCGTGTTAAATTTATCTATTAGTT
TAACTTCCTTCGCCACAGGTATCTTCATGGTGGCTGCAGGTGATATTGCTGATAAAATAGGACAACTGAGAATGACATAC
ATGGGTCTCATAATCAGTATGTTTGCATCTCTTCTATTAATTATATCGGACATCACTGCACTGCTCATCATCGGTAGAAT
TTTACAAGGTCTATCAGCAGCTATCTTGTTACCTTCAACAGTTGGCGTGTTAAATAATCAATTTAAAGGAGAACATTTAA
GACGAGCGATTAGTTATCTAATGATTAGTACTGTTGGTGGCATCGGCCTAGCTGGTGTTATCGGCGGTTTAATTGCCTCA
AATTTCGGATGGCAAACGAATTTCATCATTAGTATAGTCATTGCTTTCATTGCCATATTGCTTCTAAAAGGCACACCTGA
AAAAGTAAGTCAACATAGCCACCGTCATCCATTCGATTACAAAGGTATGTCGATTTTCGCTGTTATGATTGGTAGCTTTA
CATTATTGTTAACACAAGGATTCGAACAAGGTTGGTTTAGTACATTTTCAATCATTTGTCTGAGCATTTTTATCATCACT
ACGTTGATATTCATCATCATCGAACGTCGACATGAAGTACCTTTTATTGATTTCTCAGTATTACGCAACCGTCCGTTCAT
TGGTGCATTTTTAAATAACTTTGTTTTAAATAGCGGTCTAGGCGTAACAGTGGTCTTTTTCATATATGCTCAAACACACC
TTGGTTTATCAGCTGCGCAATCTGGACTTGTTACATTGCCATATGCCATTGTGGCAGTTGCGATGATTCGTTTAGGTGAA
AAAGCAACATTACGTTTCGGTGGCAAATTGATGCTCATCATTGGTCCGTTGTTCCCTGTCATCGGCATCACTATTATTAG
CATGACTCAATTATTGGCATCACAATATGTCATTGCAGTTATCATTGGTTTCGTCATATGTGCGATAGGTAATGGTTTAG
TCGCAACACCTGGACTTACGATTGCAATTTTCAGTATGCCTAATGAAAAAGTTGGTTTAGCTACAGGATTATATAAAATG
AGTGGTACATTAGGTGGCGCCTTTGGTATAGCACTAAGTACTACAGTTTTCAGTATGTTACAACTAAACTATGCACCAAG
TGTAGCTGCAACCGTAACATTTATAGTCAGCATTGTATTGATGATCCTTGGCTCATTGTCTGCATACATGATCATTCCAA
AAACAGTTAAATCTTAA

Upstream 100 bases:

>100_bases
TAAGGAAATCATGACTTATGTGTGAGTGAACGATCATCTATACATCCGTTCACTTCATCTCATGACTTTCTATATTTAAT
TTTTACAAGGAGTGACATCT

Downstream 100 bases:

>100_bases
ATATAATAGAAGAATTATGTTTCGAAATATCTTTATCACTTTAAAATGATATACAAGAAATCCAAGAAAAATAAGCGAAC
TGAATAAATAAAGATTCAAT

Product: drug transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTSPAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTY
MGLIISMFASLLLIISDITALLIIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAS
NFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLTQGFEQGWFSTFSIICLSIFIIT
TLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVLNSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGE
KATLRFGGKLMLIIGPLFPVIGITIISMTQLLASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVGLATGLYKM
SGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYMIIPKTVKS

Sequences:

>Translated_458_residues
MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTSPAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTY
MGLIISMFASLLLIISDITALLIIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAS
NFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLTQGFEQGWFSTFSIICLSIFIIT
TLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVLNSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGE
KATLRFGGKLMLIIGPLFPVIGITIISMTQLLASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVGLATGLYKM
SGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYMIIPKTVKS
>Mature_458_residues
MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTSPAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTY
MGLIISMFASLLLIISDITALLIIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAS
NFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLTQGFEQGWFSTFSIICLSIFIIT
TLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVLNSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGE
KATLRFGGKLMLIIGPLFPVIGITIISMTQLLASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVGLATGLYKM
SGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYMIIPKTVKS

Specific function: Multidrug efflux pump that acts independently of norA and is one of the factors that confers resistance against diverse quinolones and chemical compounds [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=374, Percent_Identity=24.5989304812834, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788710, Length=407, Percent_Identity=24.0786240786241, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87081983, Length=354, Percent_Identity=27.683615819209, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790195, Length=302, Percent_Identity=22.8476821192053, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49042; Mature: 49042

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS00962 RIBOSOMAL_S2_1 ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTSPAVLNLSISLTSFATG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEHHHHHHH
IFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALLIIGRILQGLSAAILLPST
HHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIASNFGWQTNFIISIVIAFIAIL
HHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
LLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLTQGFEQGWFSTFSIICLSIFIIT
HHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
TLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVLNSGLGVTVVFFIYAQTHLGLSAAQ
HHHHHHHHCCCCCCEEEHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHC
SGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLIIGPLFPVIGITIISMTQLLASQYVIAV
CCCCHHHHHHHHHHHHHCCCCCEEEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIGFVICAIGNGLVATPGLTIAIFSMPNEKVGLATGLYKMSGTLGGAFGIALSTTVFSML
HHHHHHHHHCCCEEECCCCEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
QLNYAPSVAATVTFIVSIVLMILGSLSAYMIIPKTVKS
HHCCCCHHHHHHHHHHHHHHHHHHCCHHEEEECHHHCC
>Mature Secondary Structure
MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTSPAVLNLSISLTSFATG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEHHHHHHH
IFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALLIIGRILQGLSAAILLPST
HHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIASNFGWQTNFIISIVIAFIAIL
HHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
LLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLTQGFEQGWFSTFSIICLSIFIIT
HHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
TLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVLNSGLGVTVVFFIYAQTHLGLSAAQ
HHHHHHHHCCCCCCEEEHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHC
SGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLIIGPLFPVIGITIISMTQLLASQYVIAV
CCCCHHHHHHHHHHHHHCCCCCEEEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IIGFVICAIGNGLVATPGLTIAIFSMPNEKVGLATGLYKMSGTLGGAFGIALSTTVFSML
HHHHHHHHHCCCEEECCCCEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
QLNYAPSVAATVTFIVSIVLMILGSLSAYMIIPKTVKS
HHCCCCHHHHHHHHHHHHHHHHHHCCHHEEEECHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA