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The map label for this gene is gsiB [H]

Identifier: 57652495

GI number: 57652495

Start: 258147

End: 259622

Strand: Reverse

Name: gsiB [H]

Synonym: SACOL0217

Alternate gene names: 57652495

Gene position: 259622-258147 (Counterclockwise)

Preceding gene: 57652496

Following gene: 57652493

Centisome position: 9.24

GC content: 31.03

Gene sequence:

>1476_bases
ATGAAGTTTAAAAGACTAGCAACTATATTTTCAGCAGTATTAGTGTTATCAGGATGTGGCTCAATGCATTCATCTGGCAA
AGACTTAAATATTTCGTTACCGTTAAAAACAAAGTCTATTGCACCTTATGAAACAGATGTACCAGTTAAGATTGGTGCTG
CAGAATCATTATTTAAAACAAATGATCAAGGAAAAATAGAAAAAGCATTAGTGAAATCGTATCATCAACCAAATGATACA
ACGTTAGATATAGAATTAAAAGATAATATTAAATTTCAAAACGGTCAAAAATTGACTGCAGAAAAAGTGAAATCTAGCCT
TGAAAATAGCATGAAAAAAAGCGACTTGGTCAAATATTCATTACCAATATCATCAATTACCGCTAAAGGTCAAAAACTGA
CAATTAAAACCAACTCCGCTTACCCTGAACTTGTATCTGAATTAGCTAATCCTTTTATGGCAATTTATGATACAGATGCT
AAATCAGATGTTAATCAAACTCCTGTTGGTACAGGCCCTTACCAAATAAAAGATTATAAGCAATCTCGAAAAATATCATT
GTCGAATTTTAAGGACTATTGGCAAGGTAAACCGAAACTTGATCATATTACTGTGACGTACCAAGAAGACGGCAATAATC
GCGTCAGAAATTTAGAATCTCAAAAAGATGATTTAATAACTGATGTCCCAGTTAATAAAGTTCAAGACATAGAAAATAAT
CAAAATTTAAAAGTGTCAAAAGAATCTGGATTTAGAACTTCTTTACTTATGTATAATCATACTAATAAAAAAATGACTAA
ATCCGTTCGTGAAGCATTAGATCATATCATTGATAGACAAGGTATTGCAGATCATATTTATCAAGGTTATGCGAAACCTG
CAACGAGTCCATTTAATGATAAAATTCCATATATTAAAGAACCTAAGTTAACCAAACAAAATATCGAACAAGCAAAAATG
TTATTAGCTAAAGATGGTTATACAAAAGAACACCCTTTAAAAATTAAGTTAATTACGTATGACGGTCGTCCAGAGCTATC
GAAAATTGCGCAAGTATTACAATCAGATGCTAAAAAAGCAAATATTGAAATCGACATTAAAAGTGTTGATGACATAGAAG
GTTACTTAAAAGACCGTTCTGCATGGGATGCAACGATGTATAGTTTCGGAACAATTCCTCGTGGAGATACAGGTTATTTC
TTTAATCAAGCATATAAAAAAGATGGTGCTATTAATAAAGGAGACTACAATAATAGCAACGTCGATGATTTAATTAATCA
ATTGAATCATACTGTAGATGTTAAGGAACGTCACAATATTTCTAATGATATTATTAAATTATCAAGTCGAGATGTACCAA
ACAGTTATATTGCTTACAACGATCAAATAGTCGCTGCTAACTCTAAGGTTAAAAATTATAAAGTAACACCTGAAGGTATC
TATTTAATCGATTATCGAACAACGATTGAACGATAA

Upstream 100 bases:

>100_bases
TAATTACTAAACTTGAACAACATTTTACTTTAACAAAATAAAGTTTTAAAATATTAATTGTTGGGTTTTACATAGTAATT
AGAAAGAGAGTGTTAATGAA

Downstream 100 bases:

>100_bases
GGGAGTGTGGGACATTGATTTATGTCTCAGCCCTGCTTCAACTATAAAAACCACGCCGAAGTCATCCAATATGATTATGG
CGTGGTTTAATTTGCTTATA

Product: ABC transporter substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYETDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDT
TLDIELKDNIKFQNGQKLTAEKVKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDA
KSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNLESQKDDLITDVPVNKVQDIENN
QNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHIIDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKM
LLAKDGYTKEHPLKIKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTIPRGDTGYF
FNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSRDVPNSYIAYNDQIVAANSKVKNYKVTPEGI
YLIDYRTTIER

Sequences:

>Translated_491_residues
MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYETDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDT
TLDIELKDNIKFQNGQKLTAEKVKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDA
KSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNLESQKDDLITDVPVNKVQDIENN
QNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHIIDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKM
LLAKDGYTKEHPLKIKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTIPRGDTGYF
FNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSRDVPNSYIAYNDQIVAANSKVKNYKVTPEGI
YLIDYRTTIER
>Mature_491_residues
MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYETDVPVKIGAAESLFKTNDQGKIEKALVKSYHQPNDT
TLDIELKDNIKFQNGQKLTAEKVKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDA
KSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNLESQKDDLITDVPVNKVQDIENN
QNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHIIDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKM
LLAKDGYTKEHPLKIKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTIPRGDTGYF
FNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDVKERHNISNDIIKLSSRDVPNSYIAYNDQIVAANSKVKNYKVTPEGI
YLIDYRTTIER

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=507, Percent_Identity=25.2465483234714, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1789887, Length=519, Percent_Identity=22.9287090558767, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1787762, Length=431, Percent_Identity=22.2737819025522, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789966, Length=350, Percent_Identity=22.5714285714286, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787551, Length=204, Percent_Identity=25.9803921568627, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 55431; Mature: 55431

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYETDVPVKIGAAESLFKT
CCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEECCHHHHHHC
NDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEKVKSSLENSMKKSDLVKYS
CCCCHHHHHHHHHCCCCCCCEEEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHCEEEE
LPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDAKSDVNQTPVGTGPYQIKDYK
CCHHHHHCCCCEEEEEECCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEECCHH
QSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNLESQKDDLITDVPVNKVQDIENN
HCCCEEHHHHHHHHCCCCCCEEEEEEEEECCCCHHHCCCCHHCCCEECCCCHHHHHCCCC
QNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHIIDRQGIADHIYQGYAKPATSPFND
CCEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCC
KIPYIKEPKLTKQNIEQAKMLLAKDGYTKEHPLKIKLITYDGRPELSKIAQVLQSDAKKA
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCHHHC
NIEIDIKSVDDIEGYLKDRSAWDATMYSFGTIPRGDTGYFFNQAYKKDGAINKGDYNNSN
EEEEEECCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCC
VDDLINQLNHTVDVKERHNISNDIIKLSSRDVPNSYIAYNDQIVAANSKVKNYKVTPEGI
HHHHHHHHCCEECCHHHCCCCHHEEEECCCCCCCCEEEECCEEEEECCCCCEEEECCCCE
YLIDYRTTIER
EEEEEHHCCCC
>Mature Secondary Structure
MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYETDVPVKIGAAESLFKT
CCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEECCHHHHHHC
NDQGKIEKALVKSYHQPNDTTLDIELKDNIKFQNGQKLTAEKVKSSLENSMKKSDLVKYS
CCCCHHHHHHHHHCCCCCCCEEEEEEECCEEECCCCCHHHHHHHHHHHHHHHHHHCEEEE
LPISSITAKGQKLTIKTNSAYPELVSELANPFMAIYDTDAKSDVNQTPVGTGPYQIKDYK
CCHHHHHCCCCEEEEEECCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEECCHH
QSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNLESQKDDLITDVPVNKVQDIENN
HCCCEEHHHHHHHHCCCCCCEEEEEEEEECCCCHHHCCCCHHCCCEECCCCHHHHHCCCC
QNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHIIDRQGIADHIYQGYAKPATSPFND
CCEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCC
KIPYIKEPKLTKQNIEQAKMLLAKDGYTKEHPLKIKLITYDGRPELSKIAQVLQSDAKKA
CCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCHHHC
NIEIDIKSVDDIEGYLKDRSAWDATMYSFGTIPRGDTGYFFNQAYKKDGAINKGDYNNSN
EEEEEECCCHHHHHHHHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCC
VDDLINQLNHTVDVKERHNISNDIIKLSSRDVPNSYIAYNDQIVAANSKVKNYKVTPEGI
HHHHHHHHCCEECCHHHCCCCHHEEEECCCCCCCCEEEECCEEEEECCCCCEEEECCCCE
YLIDYRTTIER
EEEEEHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA