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The map label for this gene is grpE [H]

Identifier: 57651974

GI number: 57651974

Start: 1668475

End: 1669101

Strand: Reverse

Name: grpE [H]

Synonym: SACOL1638

Alternate gene names: 57651974

Gene position: 1669101-1668475 (Counterclockwise)

Preceding gene: 57651975

Following gene: 57651973

Centisome position: 59.41

GC content: 31.26

Gene sequence:

>627_bases
ATGACAAATAAAGACGAATCAGTTAAAAAAAACACTGAATCAACAGTTGAAGAAACAAACGTCAAACAAAATATTGATGA
TTCAGTTGAACAAGCTGAAGAAAGTAAAGGTCATTTACAAGATGAAGCAATAGAAGAAACGTCTGACGAGAATGTTATTG
AAGAAATAGATCCAAAAGATCAAAAAATTAATGAACTTCAACAATTAGCAGATGAAAACGAAGAGAAATATTTAAGGCTC
TACGCTGAGTTTGAAAATTATAAGCGTAGAATTCAAAAAGAAAATGAAATAAACAAAACATATCAAGCACAACGTGTGTT
AACAGATATTTTACCAGCAATAGACAATATAGAACGTGCACTTCAAATTGAAGGTGATGATGAGACTTTTAAATCTCTTC
AAAAAGGTGTACAAATGGTGCATGAAAGTTTGATTAACGCACTAAAAGATAATGGTCTTGAAGTTATTAAAACTGAAGGT
GAAGCATTTGATCCAAATATTCACCAAGCTGTAGTTCAAGATGATAACCCTGATTTTGAATCTGGCGAAATCACTCAAGA
ACTACAAAAAGGATACAAGCTTAAAGATAGAGTATTAAGACCATCAATGGTCAAAGTAAACCAATAA

Upstream 100 bases:

>100_bases
ATCGCTGTGATTGGACCTACAGCTATGCATTATCAAAATGTCATTCAATTATTAAATAGAATTTGGTAATGATGGATTAG
AAAGTATTGGAGGACAGAAA

Downstream 100 bases:

>100_bases
CTTAAATTTGGCGAAAAGACATTGTTTAAAATTAAATTAATTTAATGATTAAATGGAGGAATTTTATTATGAGTAAAATT
ATTGGTATAGACTTAGGTAC

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MTNKDESVKKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAIEETSDENVIEEIDPKDQKINELQQLADENEEKYLRL
YAEFENYKRRIQKENEINKTYQAQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ

Sequences:

>Translated_208_residues
MTNKDESVKKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAIEETSDENVIEEIDPKDQKINELQQLADENEEKYLRL
YAEFENYKRRIQKENEINKTYQAQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ
>Mature_207_residues
TNKDESVKKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAIEETSDENVIEEIDPKDQKINELQQLADENEEKYLRLY
AEFENYKRRIQKENEINKTYQAQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEGE
AFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=199, Percent_Identity=29.1457286432161, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1788967, Length=172, Percent_Identity=31.3953488372093, Blast_Score=99, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17552458, Length=151, Percent_Identity=31.1258278145695, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324806, Length=142, Percent_Identity=33.0985915492958, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24653432, Length=172, Percent_Identity=29.0697674418605, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 24008; Mature: 23876

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKDESVKKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAIEETSDENVIEEIDPKD
CCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHCCCCH
QKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQAQRVLTDILPAIDNIERA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEGEAFDPNIHQAVVQDDNPDFE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHCCCCCCCC
SGEITQELQKGYKLKDRVLRPSMVKVNQ
CCHHHHHHHHCCHHHHHHCCCCHHCCCC
>Mature Secondary Structure 
TNKDESVKKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAIEETSDENVIEEIDPKD
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHCCCCH
QKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQAQRVLTDILPAIDNIERA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEGEAFDPNIHQAVVQDDNPDFE
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHCCCCCCCC
SGEITQELQKGYKLKDRVLRPSMVKVNQ
CCHHHHHHHHCCHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA