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The map label for this gene is topA

Identifier: 57651819

GI number: 57651819

Start: 1276590

End: 1278659

Strand: Direct

Name: topA

Synonym: SACOL1267

Alternate gene names: 57651819

Gene position: 1276590-1278659 (Clockwise)

Preceding gene: 57651818

Following gene: 57651820

Centisome position: 45.44

GC content: 35.07

Gene sequence:

>2070_bases
TTGGCAGATAATTTAGTCATTGTTGAATCGCCTGCAAAAGCAAAAACCATTGAAAAGTATTTAGGTAAGAAATATAAAGT
TATAGCTTCAATGGGACACGTCAGAGACTTACCAAGAAGTCAAATGGGTGTCGACACTGAAGATAATTACGAACCAAAAT
ATATAACAATACGCGGAAAAGGTCCTGTTGTAAAAGAATTGAAAAAACATGCAAAAAAAGCGAAAAACGTCTTTCTCGCA
AGTGACCCCGACCGTGAAGGTGAAGCAATTGCTTGGCATTTATCAAAAATTTTAGAGCTTGAAGATTCTAAAGAAAATCG
CGTTGTTTTCAACGAAATAACTAAAGACGCTGTTAAAGAAAGTTTTAAAAATCCTAGAGAAATTGAAATGAACTTAGTCG
ATGCACAACAAGCGCGTCGAATATTAGATAGATTAGTTGGCTATAACATCTCGCCAGTTCTTTGGAAAAAAGTAAAAAAA
GGGTTGTCAGCGGGTCGAGTTCAATCTGTTGCACTTCGTTTAGTCATTGACCGTGAAAATGAAATTCGAAACTTTAAACC
AGAAGAATATTGGACTATTGAAGGAGAATTTAGATACAAAAAATCAAAATTCAATGCTAAATTCCTTCATTATAAAAATA
AACCTTTTAAACTAAAAACGAAAAAAGATGTTGAGAAAATTACAGCTGCATTAGATGGAGATCAATTCGAAATTACAAAC
GTGACTAAAAAAGAAAAAACGCGTAATCCAGCAAACCCATTTACAACTTCTACATTACAACAAGAGGCGGCACGTAAATT
AAACTTTAAAGCAAGAAAAACAATGATGGTCGCACAACAATTATATGAAGGTATAGATTTGAAAAAACAAGGTACGATTG
GTTTAATAACATATATGAGAACCGATTCTACACGTATTTCAGATACTGCCAAAGTTGAAGCAAAACAGTATATAACTGAT
AAATACGGTGAATCTTACACTTCTAAACGTAAAGCATCAGGGAAACAAGGTGACCAAGATGCCCATGAGGCTATTAGACC
TTCAAGTACTATGCGTACGCCAGATGATATGAAGTCATTTTTGACGAAAGACCAATACCGATTATACAAATTAATTTGGG
AACGATTTGTTGCTAGTCAAATGGCTCCAGCAATACTTGATACAGTCTCATTAGACATAACACAAGGTGACATTAAATTT
AGAGCGAATGGTCAAACAATCAAGTTTAAAGGATTTATGACACTTTATGTAGAAACTAAAGATGATAGTGATAGCGAAAA
GGAAAATAAACTGCCTAAATTAGAGCAAGGTGATAAAGTCACAGCAACTCAAATTGAACCAGCTCAACACTATACACAAC
CACCTCCAAGATATACTGAGGCGAGATTAGTAAAAACACTAGAAGAATTGAAAATTGGGCGACCATCAACTTATGCACCG
ACAATAGATACGATTCAAAAGCGTAACTATGTCAAATTAGAAAGTAAGCGTTTTGTTCCTACTGAGTTGGGAGAAATAGT
TCATGAACAAGTGAAAGAATACTTCCCAGAGATTATTGATGTGGAATTCACAGTGAATATGGAAACGTTACTTGATAAGA
TTGCAGAAGGCGACATTACATGGAGGAAAGTAATCGACGGTTTCTTTAGTAGCTTTAAACAAGATGTTGAACGTGCTGAA
GAAGAGATGGAAAAGATTGAAATCAAAGATGAGCCAGCCGGTGAAGACTGTGAAATTTGTGGTTCTCCTATGGTTATAAA
AATGGGACGCTATGGTAAGTTCATGGCTTGCTCAAACTTCCCGGATTGTCGTAATACAAAAGCGATAGTTAAGTCTATTG
GTGTTAAATGTCCAAAATGTAATGATGGTGACGTCGTAGAAAGAAAATCTAAAAAGAATCGTGTCTTTTATGGATGTTCG
AAATATCCTGAATGCGACTTTATCTCTTGGGATAAGCCGATTGGAAGAGATTGTCCAAAATGTAACCAATATCTTGTTGA
AAATAAAAAAGGCAAGACAACACAAGTAATATGTTCAAATTGCGATTATAAAGAGGCAGCGCAGAAATAA

Upstream 100 bases:

>100_bases
ACAGGGAAAATGATTTGTTTAGATATAAAACGTTGACAAAAGCAAAATTAAGCGTTTATCATTTATCTTTAGTAATTAGA
TTAGCGAGGGGGAAATGACA

Downstream 100 bases:

>100_bases
TATTTTTATTTCCTAGATACATTTTAAGATTGTTAAATAGAATCATTAGTGAATCTTATTTTAAAGATAGTAAAGGATTA
ATCTAAATAAGTGCGGATAA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme

Number of amino acids: Translated: 689; Mature: 688

Protein sequence:

>689_residues
MADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLA
SDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKVKK
GLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPFKLKTKKDVEKITAALDGDQFEITN
VTKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKVEAKQYITD
KYGESYTSKRKASGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVSLDITQGDIKF
RANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPAQHYTQPPPRYTEARLVKTLEELKIGRPSTYAP
TIDTIQKRNYVKLESKRFVPTELGEIVHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAE
EEMEKIEIKDEPAGEDCEICGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCNDGDVVERKSKKNRVFYGCS
KYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSNCDYKEAAQK

Sequences:

>Translated_689_residues
MADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLA
SDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKVKK
GLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPFKLKTKKDVEKITAALDGDQFEITN
VTKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKVEAKQYITD
KYGESYTSKRKASGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVSLDITQGDIKF
RANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPAQHYTQPPPRYTEARLVKTLEELKIGRPSTYAP
TIDTIQKRNYVKLESKRFVPTELGEIVHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAE
EEMEKIEIKDEPAGEDCEICGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCNDGDVVERKSKKNRVFYGCS
KYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSNCDYKEAAQK
>Mature_688_residues
ADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLAS
DPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKVKKG
LSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPFKLKTKKDVEKITAALDGDQFEITNV
TKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKVEAKQYITDK
YGESYTSKRKASGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVSLDITQGDIKFR
ANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPAQHYTQPPPRYTEARLVKTLEELKIGRPSTYAPT
IDTIQKRNYVKLESKRFVPTELGEIVHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEE
EMEKIEIKDEPAGEDCEICGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCNDGDVVERKSKKNRVFYGCSK
YPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSNCDYKEAAQK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Homo sapiens, GI10835218, Length=625, Percent_Identity=25.76, Blast_Score=140, Evalue=5e-33,
Organism=Homo sapiens, GI4507635, Length=556, Percent_Identity=24.1007194244604, Blast_Score=121, Evalue=3e-27,
Organism=Escherichia coli, GI1787529, Length=753, Percent_Identity=38.9110225763612, Blast_Score=522, Evalue=1e-149,
Organism=Escherichia coli, GI1788061, Length=632, Percent_Identity=22.626582278481, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI48994931, Length=110, Percent_Identity=36.3636363636364, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI32563869, Length=588, Percent_Identity=25.6802721088435, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17555378, Length=504, Percent_Identity=23.6111111111111, Blast_Score=115, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6323263, Length=503, Percent_Identity=23.6580516898608, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24585251, Length=642, Percent_Identity=22.8971962616822, Blast_Score=142, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24640096, Length=519, Percent_Identity=23.121387283237, Blast_Score=104, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOP1_STAA3 (Q2FHI8)

Other databases:

- EMBL:   CP000255
- RefSeq:   YP_493840.1
- HSSP:   P46799
- ProteinModelPortal:   Q2FHI8
- SMR:   Q2FHI8
- STRING:   Q2FHI8
- EnsemblBacteria:   EBSTAT00000038620
- GeneID:   3915296
- GenomeReviews:   CP000255_GR
- KEGG:   saa:SAUSA300_1143
- eggNOG:   COG0550
- GeneTree:   EBGT00050000024420
- HOGENOM:   HBG512935
- ProtClustDB:   PRK05582
- GO:   GO:0005694
- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171
- Gene3D:   G3DSA:1.10.460.10
- Gene3D:   G3DSA:2.70.20.10
- PANTHER:   PTHR11390
- PRINTS:   PR00417
- SMART:   SM00437
- SMART:   SM00436
- SMART:   SM00493
- TIGRFAMs:   TIGR01051

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom; SSF56712 Topo_IA_core

EC number: =5.99.1.2

Molecular weight: Translated: 79114; Mature: 78982

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: ACT_SITE 298-298

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIRGK
CCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEEECC
GPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAVKE
CCHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
SFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKVKKGLSAGRVQSVALRLVIDREN
HHCCCCEEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHEEEEEEECCCH
EIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPFKLKTKKDVEKITAALDGDQFEITN
HHCCCCCCCEEEECCEEEEECCCCCEEEEEECCCCEEECCHHHHHHHHHHHCCCCEEEEC
VTKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMVAQQLYEGIDLKKQGTIGLITYMR
CHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEE
TDSTRISDTAKVEAKQYITDKYGESYTSKRKASGKQGDQDAHEAIRPSSTMRTPDDMKSF
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHHHHHH
LTKDQYRLYKLIWERFVASQMAPAILDTVSLDITQGDIKFRANGQTIKFKGFMTLYVETK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEECCCEEEEEEEEEEEEEEC
DDSDSEKENKLPKLEQGDKVTATQIEPAQHYTQPPPRYTEARLVKTLEELKIGRPSTYAP
CCCCCHHHHCCCCCCCCCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
TIDTIQKRNYVKLESKRFVPTELGEIVHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDIT
CHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHCHHHEEEEEEECHHHHHHHHHCCCCH
WRKVIDGFFSSFKQDVERAEEEMEKIEIKDEPAGEDCEICGSPMVIKMGRYGKFMACSNF
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHCCCCEEEEECCCCCEEEECCC
PDCRNTKAIVKSIGVKCPKCNDGDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPK
CCCCCHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCH
CNQYLVENKKGKTTQVICSNCDYKEAAQK
HHHHHHCCCCCCEEEEEECCCCHHHHHCC
>Mature Secondary Structure 
ADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQMGVDTEDNYEPKYITIRGK
CCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEEECC
GPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILELEDSKENRVVFNEITKDAVKE
CCHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
SFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKVKKGLSAGRVQSVALRLVIDREN
HHCCCCEEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHEEEEEEECCCH
EIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPFKLKTKKDVEKITAALDGDQFEITN
HHCCCCCCCEEEECCEEEEECCCCCEEEEEECCCCEEECCHHHHHHHHHHHCCCCEEEEC
VTKKEKTRNPANPFTTSTLQQEAARKLNFKARKTMMVAQQLYEGIDLKKQGTIGLITYMR
CHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEE
TDSTRISDTAKVEAKQYITDKYGESYTSKRKASGKQGDQDAHEAIRPSSTMRTPDDMKSF
CCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHHHHHH
LTKDQYRLYKLIWERFVASQMAPAILDTVSLDITQGDIKFRANGQTIKFKGFMTLYVETK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEECCCEEEEEEEEEEEEEEC
DDSDSEKENKLPKLEQGDKVTATQIEPAQHYTQPPPRYTEARLVKTLEELKIGRPSTYAP
CCCCCHHHHCCCCCCCCCCEEEEECCHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCC
TIDTIQKRNYVKLESKRFVPTELGEIVHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDIT
CHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHCHHHEEEEEEECHHHHHHHHHCCCCH
WRKVIDGFFSSFKQDVERAEEEMEKIEIKDEPAGEDCEICGSPMVIKMGRYGKFMACSNF
HHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHCCCCEEEEECCCCCEEEECCC
PDCRNTKAIVKSIGVKCPKCNDGDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPK
CCCCCHHHHHHHHCCCCCCCCCCCHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCCCCH
CNQYLVENKKGKTTQVICSNCDYKEAAQK
HHHHHHCCCCCCEEEEEECCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA